Distribution of Helicobacter pylori in north China

Table 1 PCR primers for amplification of cagA, vacA and iceA sequences.

Gene and DNA region	Prime	Primer sequence (5’-3’)	Size of PCR product (bp)	References
cagA	CAGAF	GGCAATGGTGGTCCTGGAGCTAGGC	324	Pan[21]
	CAGAR	GGAAATCTTTAATCTCAGTTCGG
vacAs1 and s2	VA1-F	ATGGAAATACAACAAACACAC	259/286	Atherton[22]
	VA1-R	CTGCTTGAATGCGCCAAAC
m1a	VA3-F	GGTCAAAATGCGGTCATGG	290	Atherton[22]
	VA3-R	CCATTGGTACCTGTAGAAAC
m1b	VAm-F3	GGCCCCAATGCAGTCATGGAT	291	Atherton[23]
	VAm-R3	GCTGTTAGTGCCTAAAGAAGCAT
m2	VA4-F	GGAGCCCCAGGAAACATTG	352	Atherton[22]
	VA4-R	CATAACTAGCGCCTTGCAC
iceA1	iceA1F	GTGTTTTTAACCAAAGTATC	247	Peek[10]
	iceA1R	CTATAGCCASTYTCTTTGCA
iceA2	iceA2F	GTTGGGTATATCACAATTTAT	229/334	Peek[10]
	iceA2R	TTRCCCTATTTTCTAGTAGGT

R = A/G.