Inhibition of PMA-induced endothelial cell activation and adhesion by over-expression of domain negative IκBα protein

Figure 2 Down-regulation of PMA-induced adhesion molecules expression in ECV cells transfected with pCMV-IκBαM compared with pCMV-IκBα. RT-PCR analysis was performed with primers specific for ICAM-1 (A) and VCAM-1 (B). C: The expression of ICAM-1, VCAM-1 and P-selectin were detected using specific antibodies by flow cytometry. Results are representative of at least three independent experiments.