High level of hepatitis B virus DNA after HBeAg-to-anti-HBe seroconversion is related to coexistence of mutations in its precore and basal core promoter

doi:10.3748/wjg.v11.i20.3131

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May 28, 2005 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2005; 11(20): 3131-3134
Published online May 28, 2005. doi: 10.3748/wjg.v11.i20.3131

Table 1 Primers and probes for detections of G1896A or A1762T/G1764A mutations.

Denomination	Sequences (5’-3’)
PCP	BIO -GAGAC TCTAA GGCTT CTCGA TACAG AGCTG AGG
PCMd	DIG -CTCAC GCTAC ATTGT GTGCC TTGGG TGGCT TCA
PCWd	FITC -GTCCG TAGTC TCGTT GTGCC TTGGG TGGCT TGG
BCP1-M	DIG -GCTGA CGATG CGATG GGGAG GAGAT TAGGT TAATG A-3’
BCP1-W	FITC -CGTCC GTAGT GCCGA GGGAG GAGAT TAGGT TAAAG G-3’
PCA	CCCAG CAGAG AATGG CTTGC CTGAG TGCAG TATG
PCSc	CCCGA ATTCC ACCGT GAACG CCCAT CAG
PCAc	CCCAA GCTTG CAGTA TGGTG AGGTG AGCAA TG

BIO: the abbreviation of biotin; DIG: the abbreviation of digoxigenin; FITC: the abbreviation of fluorescein isothiocyanate. The underlined nucleotides had no relationship with HBV.

Citation: Peng XM, Huang GM, Li JG, Huang YS, Mei YY, Gao ZL. High level of hepatitis B virus DNA after HBeAg-to-anti-HBe seroconversion is related to coexistence of mutations in its precore and basal core promoter. World J Gastroenterol 2005; 11(20): 3131-3134
URL: https://www.wjgnet.com/1007-9327/full/v11/i20/3131.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i20.3131