Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells

Figure 3 Positive expression occurred in cell nucleoli, with brown or yellow dye, of PCNA protein of GBC-SD cells (immunohistochemistry SABC method, ×200). A: The brown dye of PCNA was shown positively in most cells of the control group. B: In the experiment group with treatment of IC₅₀ NCTD for 48 h, the positive cells of PCNA expression decreased significantly and the dye in cell nucleoli became light and shallow.