Simplified purification method for Clostridium difficile toxin A

Figure 1 Purification of Clostridium difficile toxin A by DEAE-Toyopearl 650 mol/L chromatography. Fractions 1-34 were eluted with 50 mmol/L Tris-HCl buffer (pH7.5) containing a linear NaCl gradient (50 mmol/L-250 mmol/L), then fractions 35-50 were eluted with 300 mmol/L NaCl and fractions 51-75 were eluted with linear gradient (300 mmol/L-600 mmol/L NaCl) in the same buffer.