Copyright
©The Author(s) 2004.
World J Gastroenterol. Sep 15, 2004; 10(18): 2756-2758
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2756
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2756
Table 1 Purification of C. difficile toxin A
| Purification step | Vol (mL) | Protein(mg/mL) | Lethal dose(Mice, MLD/mL) | Cytotoxicity (CU/mg) |
| Crude culture supernatant | 200 | 1.86 | 6 × 104 | 1010 |
| 50% (NH4)2SO4 precipitation | 10 | ND | ND | ND |
| Acid precipitation | 10 | 6.15 | 2 × 106 | 1010 |
| DEAE-Toyopeal 650 mol/L | 6 | 0.881 | 1 × 106 | 107 |
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Citation: Fu SW, Xue J, Zhang YL, Zhou DY. Simplified purification method for
Clostridium difficile toxin A. World J Gastroenterol 2004; 10(18): 2756-2758 - URL: https://www.wjgnet.com/1007-9327/full/v10/i18/2756.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i18.2756