Angiogenic synergistic effect of basic fibroblast growth factor and vascular endothelial growth factor in an in vitro quantitative microcarrier-based three-dimensional fibrin angiogenesis system

Figure 3 Time course of HMVEC angiogenesis in fibrin. Microcarriers coated with HMVECs were embedded in fibrin. The angiogenic effects of either bFGF or VEGF were dose-dependent over the range from 10 to 40 ng/mL. At day 1, 10 ng/mL of bFGF and VEGF induced angiogenesis with an ALS of 32.13 ± 16.6 μm and 43.75 ± 27.92 mm, respectively, which were significantly higher than that of the control (5.88 ± 4.45 mm, P < 0.01). The difference became more significant, as the time increased.