Differential expression of cholangiocyte and ileal bile acid transporters following bile acid supplementation and depletion

Figure 2 Effect of TCA and CY feeding on the expression of ASBT and t-ASBT protein in cholangiocytes. Bar graphs show-ing densitometric ratios of ASBT and t-ASBT standardized to β-actin in PNS fractions of freshly isolated cholangiocytes from rats fed control (C), 1% TCA or 5% CY incorporated diet. A 80 μg for ASBT and 120 μg for t-ASBT PNS protein were sub-jected to SDS-PAGE, immunoblotted for ASBT (48 kDa), t-ASBT (19 kDa) and β-Actin (41 kDa) and the bands were then visualized using enhanced chemiluminescence techniques. Densitometric values shown here are expressed as mean ± SE (^aP < 0.05) and represent 3 feeding studies for ASBT protein analyses of the TCA and CY groups. Similarly, 3 feeding studies were per-formed to reflect the data obtained on t-ASBT protein follow-ing CY feeding, but only 2 feeding studies were done for t-ASBT protein analysis of the TCA group. Ten rats per group were analyzed in each of these feeding studies, thus a total of 30 rats per feeding study were used in all of the above groups, whereas 20 rats were incorporated into the analysis of t-ASBT in the TCA group.