Nimesulide inhibits proliferation via induction of apoptosis and cell cycle arrest in human gastric adenocarcinoma cell line

Figure 1 Effects of various concentration of nimesulide on the proliferation of SGC-7901 cells for different time periods analysing by cell counts (n = 3).

Figure 2 Effects of nimesulide on SGC-7901 cells analyzing the cell survival using the MTT assay.

Figure 3 (A) control group: Microvilli of SGC-7901 cells were enrichment, nucleus was large and round, chromatin was disperse, nucleolus was distinct. TEM × 6000. (B) Treated with nimesulide at the concentration of 400 μmol/L for 72 h: Disap-pearance of microvilli, margination of nuclear chromatin, the organelle swelled up and physallization. TEM × 8000.

Figure 4 The results of flow cytometry analysis of SGC-7901 cells treated with nimesulide for 72 h and their DNA content was determined by flow cytometry. A: DNA histogram: (a) control, (b) 50 μmol/L nimesulide, (c) 100 μmol/L nimesulide, (d) 200 μmol/L nimesulide, (e) 400 μmol/L nimesulide. B: The apoptosis percentage of nimesulde-treated SGC-7901 cells.

Figure 5 (A) Positive staing of P27^kip1 protein was mainly in cytoplasm of SGC-7901 cells before treated with nimesulide. (B) Strong positive staing of P27^kip1 protein was in cytoplasm and in nuclei of of SGC-7901 cells after treated with nimesulide at the concentration of 400 μmol/L. SP × 200.

Figure 6 Effects of nimesulide on P27^kip1 proteins expression in SGC-7901 cells. The data present ^-x±s, n = 3. ^bP < 0.01 vs control.