Juvenile ferric iron prevents microbiota dysbiosis and colitis in adult rodents

Figure 1 Effect of a 6 wk juvenile ingestion of ferrous (150 mg/kg per day po) vs ferric (150 mg/kg per day po) iron on the inflammatory response at adulthood to a trinitrobenzene sulfonic acid-induced colitis in mice. A: Macroscopic damage scores in non-inflamed (sham-colitis) and inflamed (trinitrobenzene sulfonic acid, TNBS) mice; B: Colonic myeloperoxidase (MPO) activity in non-inflamed (sham-colitis) and inflamed (TNBS) mice. Data are expressed as mean ± SE (n = 8 per group). ^aP < 0.05 vs TNBS-treated group, ^bP < 0.01 vs Sham-colitis.

Figure 2 Effect of a 6 wk juvenile ferric iron administration (75 and 150 mg/kg per day po) on the inflammatory response at adulthood to a trinitrobenzene sulfonic acid-induced colitis in mice. A: Macroscopic damage scores in non-inflamed (sham-colitis) and inflamed trinitrobenzene sulfonic acid (TNBS) mice; B: Colonic myeloperoxidase (MPO) activity in non-inflamed (sham-colitis) and inflamed (TNBS) mice. Data are expressed as mean ± SE (n = 8 per group). ^aP < 0.05, ^bP < 0.01 vs TNBS-treated group, ^dP < 0.01 vs Sham-colitis.

Figure 3 Effect of a juvenile ferric iron administration for 6 wk on the microflora profile of mice and its evolution consecutively to a trinitrobenzene sulfonic acid-induced colitis. A: Total Flora; B: Firmicutes; C: Bacteroidetes; D: Enterobacteria. Iron75: Ferric iron 75 mg/kg/d po - 6 wk; Iron150: Ferric iron 150 mg/kg per day po - 6 wk. Data are expressed as mean ± SE (n = 8 per group). ^aP < 0.05, ^bP < 0.01 vs Sham-colitis; ^dP < 0.01 vs TNBS-treated group.

Figure 4 Effect of a 6 wk juvenile ferric iron administration (75 mg/kg per day po) on the microflora profile of rats and its evolution consecutively to a trinitrobenzene sulfonic acid colitis. A: Total Flora; B: Firmicutes; C: Bacteroidetes; D: Enterobacteria; Iron75: Ferric iron 75 mg/kg per day po - 6 wk; Iron150: Ferric iron 150 mg/kg per day po - 6wk. Data are expressed as mean ± SE (n = 8 per group). ^aP < 0.05, ^bP < 0.01 vs Sham-colitis.

Figure 5 Effect of a 6 wk juvenile ferric iron supplementation (75 mg/kg per day po) on the inflammatory response at adulthood of rats submitted or not (sham-colitis) to a trinitrobenzene sulfonic acid-induced colitis. A: Macroscopic damage scores (MDS); B: Myeloperoxidase (MPO) activity Data are expressed as mean ± SE (n = 8 per group). ^aP < 0.05 vs trinitrobenzene sulfonic acid (TNBS)-treated group, ^bP < 0.01 vs Sham-colitis.