Reversal of the phenotype by K-rasval12 silencing mediated by adenovirus-delivered siRNA in human pancreatic cancer cell line Panc-1

Figure 1 Schematic outline of siRNA expressing adenovirus AdH1-K-ras^val12, AdH1-p53 and AdH1-empty.

Figure 2 Foci produced by adenoviruses on d 2-7 and 12 after transfection of 293 cells.

Figure 3 Sequence (for detail, refer to[46]) of Val¹² mutant alleles of human K-ras and the predicted mutant-specific short hairpin transcripts encoded by AdH1-K-ras^val12.

Figure 4 Representative Western blot analysis for K-ras^val12 expression in Panc-1 and HeLa cells 60 h after infection with viral stocks (AdH1-K-ras^val12, AdH1-empty, AdH1-p53) of three separate experiments.

Figure 5 Northern blot analysis with total RNA from same infected Panc-1 cells of single experiment.

Figure 6 Apoptotic indices shown as the mean of four separate experiments for each group (P<0. 05).

Figure 7 Stable polyclonal pools of Panc-1 and HeLa cells harboring wild-type H-ras infected with the indicated viral stocks and immunoblotted for the detection of p53 and actin proteins in single experiment.

Figure 8 Detection of K-ras^val12 at protein level in Panc-1 and HeLa cells without virus infection in single experiment.