Effects of PI3K and p42/p44 MAPK on overexpression of vascular endothelial growth factor in hepatocellular carcinoma

Figure 1 Cobalt chloride stimulated the expression of VEGF mRNA in HepG₂ cells in a concentration-dependant manner.

Figure 2 rhEGF stimulated the expression of VEGF mRNA in HepG₂ cells in a concentration-dependant manner.

Figure 3 Negative expression of VEGF protein in HepG₂ cells cultured in DMEM without serum. Immunocytochemistry ×400.

Figure 4 Expression of VEGF protein in HepG₂ cells stimulated by cobalt chloride (400 μmol/L, 24 h) immunocytochemistry ×400.

Figure 5 Expression of VEGF protein in HepG₂ cells stimu-lated by rhEGF(25 ng/mL, 24 h) immunocytochemistry ×400.

Figure 6 LY294002 inhibited the expression of VEGF mRNA in HepG₂ cells stimulated by cobalt chloride.

Figure 7 PD98059 had no effect on the expression of VEGF mRNA in HepG₂ cells stimulated by cobalt chloride.