Probiotics inhibit TNF-α-induced interleukin-8 secretion of HT29 cells

Figure 1 Concentrations of IL-8 in each group (mean ± SD). Natural interleukin-8 expression was seldom found in HT29 cells of control group. When stimulated by TNF-α (10 ng/ml), HT29 cells secreted a large number of IL-8, and the concentra-tions IL-8 in groups T, B and L were significantly increased than that in control (P < 0.001). However, there was some dif-ference in the concentration of IL-8 among groups T, B and L. There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus in groups B and L compared with group T (P = 0.002, 0.01, respectively), but there was no difference in concentration of IL-8 between groups B and L (P = 0.068).

Figure 2 Expression of nuclear NF-κB p65 in each group. Line 1: group T, Line 2: control, Line 3: group L, Line 4: group B.