Herpes simplex virus thymidine kinase and ganciclovir suicide gene therapy for human pancreatic cancer

Figure 1 Electrophoretic results of PCR products. 1. GINaTK, 2. PA317/TK, 3. Marker (puc19DNA/MSP I).

Figure 2 Results of scanning electron microscop. A: The sur-face of PA317 cell are very smooth, B: There are a lot of bumps on the surface of PA317/TK cells because these gene- modified cells are secreting virus particles.

Figure 3 Results of RT-PCR. 1. SW1990, 2. SW1990/TK, 3. GINaTK, 4. Marker.

Figure 4 Typical morphological changes in SW1990/TK cells when exposed to GCV (50 μg/ml). A: SW1990/TK cells with-out GCV. B: Two days after adding 50 μg/ml of GCV, cells became round and smaller, losing their normal morphology. C: Four days after adding GCV, cells gathered to balls. D: Five days later, cells clasped into small fragments.

Figure 5 Sensitivity of SW1990/TK to GCV.

Figure 6 Bystander effects of SW1990/TK cell line.