Activation of human colon mast cells through proteinase activated receptor-2

Figure 1 Effects of tc-LIGRLO, tc-OLRGIL, SLIGKV, VKGILS, trypsin, anti-IgE and calcium ionophore A23187 (CI) on tryptase release from colon mast cells. The values shown are mean ± SEM for four separate experiments. Stimulus or control was incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with buffer alone group (paired Student’s t test).

Figure 2 Effects of tc-LIGRLO, tc-OLRGIL, SLIGKV, VKGILS, trypsin, anti-IgE and calcium ionophore A23187 (CI) on histamine release from colon mast cells. The values shown are mean ± SEM for four separate experiments. Stimulus or control was incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with buffer alone group (paired Student’s t test).

Figure 3 Time course for tc-LIGRLO (100 µM) induced release of tryptase (─■─) and histamine (─●─) from colon mast cells. Data shown are mean ± SEM of four separate experiments.