Three new alternative splicing variants of human cytochrome P450 2D6 mRNA in human extratumoral liver tissue

Figure 1 Electrophoresis of RT-PCR products of CYP2D6 cDNA. Lane 1: 1 kb DNA marker, 2: Amplification of 1.1 kb in size, 3: Amplification of full length of 1.57 kb.

Figure 2 Partial sequences of the cloned human CYP2D6 cDNA. The upper sequence represents the sense strand and the underside sequence represents the sequenced anti-sense strand.

Figure 3 Comparison of sequences of CYP2D6 cDNA, CYP2D7 BP and 3 new CYP2D6 alternative splicing variants, f, g, h. The different bases compared with wild type CYP2D6 are indicated in boldface and “_”, and the border of exons is indicated by ‘!’. The skipped parts are indicated by ‘-’.

Figure 4 Representative electrophoresis of RT-PCR products of exons 1 to 4 of CYP2D6 from human liver tissues and HepG2 cells with beta-actin as internal control (464 bp). Lane 1: 100 bp marker, lane 2: A sample having both full length of 700 bp and shorter 300 bp, lane 3: A sample having only shorter ones (300 bp), lane 4: HepG2 cells having no CYP2D6 expressed, Lane 5: A sample having only full length 700 bp of exons 1 to 4 of CYP2D6.

Figure 5 Partial sequences of two alternative splicing variants of CYP2D6. A: Part of skipped exon 1, exon 2, exon 3, and part of exon 4. B: Skipped exon 3.

Figure 6 Electrophoresis of PCR products from genomic DNA amplified using CYP2D6 F and CYP2D6 4R primers. Lane 1: λ/EcoR I and Hind III marker, lanes 2-4: PCR products from livers expressing full and short length RT-PCR products, Lanes 5, 6: PCR products from livers expressing full length RT-PCR products, Lanes 7, 8: PCR products from livers expressing only short length RT-PCR products.