Expression of survivin in primary and metastatic gastric cancer cells obtained by laser capture microdissection

Figure 1 Laser capture microdissection of cells from gastric carcinoma. A: Morphology map view for pathological diagnosis (× 100). B: Group of gastric carcinoma cells were selected for LCM (× 200). C: The same section revealed where carcinoma cells were lifted from the section (× 200). D: The carcinoma cells were removed on the plastic film to provide a template for PCR.

Figure 2 Representative RT-PCR results of survivin expres-sion in normal (N), tumor (T) and metastatic carcinoma (M) cells obtained by LCM from 3 patients suffering gastric cancer. Case 1 was a patient without lymphatic metastasis, cases 2 and 3 were patients with lymphatic metastasis. G: Genomic DNA from human peripheral blood. W: Water as negative control.