Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients

doi:10.3748/wjg.v16.i47.5965

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Dec 21, 2010 (publication date) through May 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 21, 2010; 16(47): 5965-5974
Published online Dec 21, 2010. doi: 10.3748/wjg.v16.i47.5965

Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients

Aikaterini Tsouma, Chrysanthi Aggeli, Panagiotis Lembessis, George N Zografos, Dimitris P Korkolis, Dimitrios Pectasides, Maria Skondra, Nikolaos Pissimissis, Anastasia Tzonou, Michael Koutsilieris

Aikaterini Tsouma, Chrysanthi Aggeli, Maria Skondra, Nikolaos Pissimissis, Michael Koutsilieris, Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, 11527, Athens, Greece

Chrysanthi Aggeli, George N Zografos, Third Department of Surgery, Athens General Hospital “G. Gennimatas”, 11527, Athens, Greece

Chrysanthi Aggeli, Dimitris P Korkolis, Department of Surgical Oncology, Hellenic Anticancer Institute, “Saint Savvas” Hospital, 11522, Athens, Greece

Panagiotis Lembessis, Endo/OncoResearch Medical Center, Platia Mavilis, Ampelokipi, 11521, Athens, Greece

Dimitrios Pectasides, Maria Skondra, Second Department of Internal Medicine, Propaedeutic Oncology Section, University of Athens, Attikon University Hospital, 12462, Athens, Greece

Anastasia Tzonou, Department of Hygiene, Epidemiology and Medical Statistics, Medical School, National and Kapodistrian University of Athens, 11527, Athens, Greece

Author contributions: Tsouma A, Aggeli C, Lembessis P and Koutsilieris M developed the concept of the multiplex PCR-based technique, performed data analysis and wrote the present manuscript; Aggeli C, Zografos GN, Korkolis DP, Pectasides D, Skondra M and Pissimissis N collected blood samples and clinical data, evaluated the clinical data, performed data analysis and participated in the writing of the manuscript; Tzonou A performed statistical analysis and participated in the writing of the manuscript.

Supported by The Ministry of Development of the Greek Government (GGET-AKMON)

Correspondence to: Michael Koutsilieris, MD, PhD, Professor and Chairman, Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Micras Asias 75, Goudi-Athens, 11527, Athens, Greece. mkoutsil@med.uoa.gr

Telephone: +30-210-7462597 Fax: +30-210-7462571

Received: May 17, 2010
Revised: July 12, 2010
Accepted: July 19, 2010
Published online: December 21, 2010

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