Basic Study
Copyright ©The Author(s) 2019.
World J Methodol. Nov 14, 2019; 9(3): 32-43
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32
Table 1 Yield and purity of the recovered DNA from various methods
MethodSample size, nTissue deparaffinizationDigestion periodProteinase K inactivationDNA yield range in ng/µLDNA purity range, A260/280
120Xylene24 hNo7.65-45.231.42-2.01
220Xylene24 hYes7.53-33.71.34-1.94
320Xylene72 hNo23.68-208.311.68-1.89
420Xylene72 hYes13-149.411.54-1.93
520Mineral oil72 hNo11.74-86.741.41-1.74
Table 2 Statistical comparison of the DNA yield among various methods
Sl No.Unpaired t-testP valueR valueSignificance
1Method 1 vs Method 20.9080.0003NS
2Method 1 vs Method 3< 0.00010.401c
3Method 1 vs Method 40.00170.23b
4Method 1 vs Method 50.5240.01NS
5Method 2 vs Method 3< 0.00010.418c
6Method 2 vs Method 40.00110.248b
7Method 2 vs Method 50.3930.019NS
8Method 3 vs Method 40.130.059NS
9Method 3 vs Method 5< 0.00010.366c
10Method 4 vs Method 50.0040.192b