Copyright ©The Author(s) 2021.
World J Methodol. Sep 20, 2021; 11(5): 243-262
Published online Sep 20, 2021. doi: 10.5662/wjm.v11.i5.243
Table 1 In vitro applications of polyclonal IgYs
Target biomolecule(s)
In vitro immunochemical technique
Proposed field of application
Major surface antigen of Toxoplasma gondii (SAG1)Latex agglutination assayDiagnosis of ToxoplasmosisCakir-Koc et al[132], 2020
Protein A of Staphylococcus aureusImmunocapture PCR assayDetection of Staphylococcus aureus in food samples, skin and nasal swabsKota et al[133], 2020
Peptides/proteins present in detoxified western Russell’s viper venomPaper-based microfluidic immunochromatographic testDifferential diagnosis of Russell’s viper envenomationLin et al[134], 2020
SARS-CoV-2 antigenFluorescence immunochromatographic rapid-antigen testDiagnosis of COVID-19Porte et al[79], 2020
Antigens present in total saline extract of Taenia crassiceps metacestodesELISADetection of neurocysticercosis daSilva et al[32], 2020
Antigens present in total saline extract of Ancylostoma ceylanicumELISADiagnosis of Hookworm infectionSouza et al[135], 2020
Non-glycosylated synthetic oligopeptides of Dermatophagoides group I allergens Immuno-dot blot assay (with the use of IgY-colloidal gold nanoparticles conjugates)Detection of indoor dust mite allergensEgea et al[136], 2019
Antigens present in whole bacterial suspension of formalin- and heat- inactivatedSalmonella typhimurium and Salmonella enteritidisIn vitro immunochemicaltechniques Diagnosis of infection with Salmonella typhimurium and Salmonella enteritidisEsmailnejad et al[26], 2019
Antigenic extracts of Strongyloides venezuelensis infectious filariform larvae and parthenogenetic femalesELISADiagnosis of human strongyloidiasisdeFaria et al[33], 2019
Antigens present in total saline extract of Ascaris suum adult life formsTissue indirect immunofluorescence assay & ELISADiagnosis of human ascariasisLopes et al[31], 2019
Free prostate specific antigen ELISADiagnosis of human prostate cancerŁupicka-Słowic et al[137], 2019
Antigens (capsid proteins VP2 & VP3) present in beta-propiolactone-inactivated enterovirus 71 Fluorescence sensor assayDiagnosis of hand-foot-and-mouth disease caused by enterovirus 71 infectionNie et al[138], 2019
Fusarium verticillioides 97K exoantigenELISADetection of Fusarium verticillioides (and prediction of fumonisin contamination) in poultry feedOmori et al[139], 2019
Recombinant purified catalytic domain of Karilysin ELISAEvaluation of karilysin (i.e. an enzyme secreted by the periontopathogen Tannerella forsythia) as a biomarker for the diagnosis of periodontitisSkottrup et al[34], 2019
Fumonisin B1Lateral flow immunoassayDetection of fumonisin B1 and fumonisin B2 in maizeTran et al[140], 2019
Synthetic extracellular peptide of matrix-2 protein of influenza A virus, conserved in all strainsLatex agglutination assayDiagnosis of infection with Influenza A virus Budama-Kilinc et al[141], 2018
Sulfamethazine (SMZ)ELISA, FPIADetection of veterinary drug residues (SMZ) in milkLiang et al[142], 2018
Native calf adenosine deaminase (ADA)ELISAEvaluation of ADA as a cancer biomarkerŁupicka-Słowic et al[143], 2018
Nucleoprotein of influenza A virus Immunocytochemistry, Immunohistochemistry Diagnosis of infection with influenza A virus da Silva et al[144], 2018