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Mishra R, Singh TG, Bhatia R, Awasthi A. Unveiling the therapeutic journey of snail mucus in diabetic wound care. NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY 2025; 398:6531-6560. [PMID: 39869187 DOI: 10.1007/s00210-024-03657-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/01/2024] [Accepted: 11/19/2024] [Indexed: 01/28/2025]
Abstract
A diabetic wound (DW) is an alteration in the highly orchestrated physiological sequence of wound healing especially, the inflammatory phase. These alterations result in the generation of oxidative stress and inflammation at the injury site. This further leads to the impairment in the angiogenesis, extracellular matrix, collagen deposition, and re-epithelialization. Additionally, in DW there is the presence of microbial load which makes the wound worse and impedes the wound healing cycle. There are several treatment strategies which have been employed by the researchers to mitigate the aforementioned challenges. However, they failed to address the multifactorial pathogenic nature of the disease. Looking at the severity of the disease researchers have explored snail mucus and its components such as achacin, allantoin, elastin, collagen, and glycosaminoglycan due to its multiple therapeutic potentials; however, glycosaminoglycan (GAGs) is very important among all because they accelerate the wound-healing process by promoting reepithelialization, vascularization, granulation, and angiogenesis at the site of injury. Despite its varied applications, the field of snail mucus in wound healing is still underexplored. The present review aims to highlight the role of snail mucus in diabetic wound healing, the advantages of snail mucus over conventional treatments, the therapeutic potential of snail mucus, and the application of snail mucus in DW. Additionally, clinical trials, patents, structural variations, and advancements in snail mucus characterization have been covered in the article.
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Affiliation(s)
- Ritika Mishra
- Department of Pharmaceutics, ISF College of Pharmacy, Moga, 142001, Punjab, India
| | - Thakur Gurjeet Singh
- Chitkara College of Pharmacy, Chitkara University, Rajpura, 140401, Punjab, India
| | - Rohit Bhatia
- Chitkara College of Pharmacy, Chitkara University, Rajpura, 140401, Punjab, India.
| | - Ankit Awasthi
- Chitkara College of Pharmacy, Chitkara University, Rajpura, 140401, Punjab, India.
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Norouzi P, Rezaei Kolarijani N, Mahheidari N, Ehterami A, Bit A, Gharravi AM, Yekesadat SM, Aghayan SN, Haghi-Daredeh S, Salehi M. Design and evaluation of sodium alginate-based hydrogel containing green tea for the treatment of diabetic ulcers in rat model. J Biomater Appl 2025:8853282251345004. [PMID: 40398864 DOI: 10.1177/08853282251345004] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 05/23/2025]
Abstract
A functional and biocompatible biomaterial is essential for accelerating the regeneration of skin tissue at the wound site. Hydrogel scaffolds in three dimensions show promising candidates for this purpose. This study was conducted to design a novel porous cross-linked alginate (Alg) hydrogel containing green tea (GT) and assess its morphology, swelling, weight loss, hemocompatibility, and cytocompatibility. Ultimately, the created hydrogel's therapeutic effectiveness was examined in a complete dermal diabetes wound model. The findings indicated that the hydrogel prepared had significant porosity, with interconnected pores around 75.821 µm in size. The weight loss evaluation indicated that the created hydrogel can be degraded naturally, with a weight loss ratio of about 74% for Alg/GT 80 mg after being incubated for 24 hours. Additionally, the study indicated that hydrogel dressings exhibited greater wound closure compared to gauze-treated wounds, which served as the control. The group with GT at a concentration of 80 mg showed the highest percentage of wound closure. The histopathological studies and IHC evaluation for TGF-β1 confirmed the in vivo finding. This study proposes utilizing 3D Alg hydrogels with GT as a wound dressing, but further studies are needed.
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Affiliation(s)
- Pirasteh Norouzi
- Department of Physiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Nariman Rezaei Kolarijani
- Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Naimeh Mahheidari
- Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Arian Ehterami
- Institute for Regenerative Medicine (IREM), University of Zurich, Zurich, Switzerland
| | - Arindam Bit
- Department of Biomedical Engineering, NIT, Raipur, India
| | - Anneh Mohammad Gharravi
- Department of Basic Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | | | - Seyedeh Nazanin Aghayan
- Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Saeed Haghi-Daredeh
- Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
| | - Majid Salehi
- Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
- Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
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Moussa AK, Abd El-Rahman HA, Mohamed RR, Hanna DH. Multifunctional Plasticized Hyaluronic-Acid-Based Nanogel Dressing for Accelerating Diabetic and Nondiabetic Wounds. Biomacromolecules 2025. [PMID: 40340350 DOI: 10.1021/acs.biomac.5c00126] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 05/10/2025]
Abstract
Diabetic ulcers are associated with oxidative stress, inflammation, decreased synthesis of pro-healing mediators, and impaired vascularization, which convert the wound from acute to chronic and delay healing. An extended duration of wound healing raises the possibility of complications such as infection, sepsis, and even amputation. The objective of this study is the synthesis of a plasticized cross-linked hyaluronic acid (HA)-grafted poly(acrylamide-co-itaconic acid) nanogel as a nontoxic adhesive, swellable, antibacterial wound dressing with good mechanical properties to protect the wound from pathogens and accelerate the healing process, in addition to decreasing oxidative stress and inflammatory cytokines while increasing anti-inflammatory cytokines and angiogenesis. Nanogel H3 with a ratio (AM/IA) (3:1) showed excellent adhesion with good mechanical properties, biocompatibility, swelling, antioxidant, and antibacterial efficiencies. It showed great wound closure in vitro and in vivo with downregulation of inflammatory cytokines, upregulation of anti-inflammatory cytokines, and enhanced angiogenesis in vivo on diabetic and nondiabetic wounds.
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Affiliation(s)
- Aalaa K Moussa
- Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt
| | | | - Riham R Mohamed
- Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt
| | - Demiana H Hanna
- Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt
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Feng Z, Lu S, He Y, Sun Y, Ma L, Guo H, Kang Y, Xu R, Song Y, Huang L, Qin L, Zhang J, Zhang Y. A dual-network hydrogel patch fabricated by alginate/sulfobetaine methacrylate enriched with Dictyophora indusiata β-glucans promotes diabetic wound repair. Int J Biol Macromol 2025; 308:142495. [PMID: 40139598 DOI: 10.1016/j.ijbiomac.2025.142495] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/23/2024] [Revised: 03/04/2025] [Accepted: 03/23/2025] [Indexed: 03/29/2025]
Abstract
This study developed a dual-network hydrogel patch loaded with Dictyophora indusiata β-glucans to enhance diabetic wound healing. The hydrogel combines a flexible primary network formed by polymerized sulfobetaine methacrylate with a rigid secondary alginate network crosslinked via metal ions. The resulting material demonstrates favorable mechanical properties for wound care, achieving 600 % elongation at break, 3.12 MPa compressive strength, and 1.5 kPa tissue adhesion strength. These characteristics meet with the physical requirements necessary for effective diabetic wound management. Furthermore, the β-glucans derived from Dictyophora indusiata, which serve as the main bioactive component, endowed the hydrogel patch with significant antioxidant and anti-inflammatory properties. Cellular experiments have demonstrated that the hydrogel patch significantly reduces reactive oxygen species levels in cells and inhibits inflammatory responses. In animal wound model, diabetic wound treated with a hydrogel patch achieved a closure rate of 98.26 % by the second week. Additionally, histological analyses revealed that the hydrogel patch significantly facilitates angiogenesis, collagen deposition, and re-epithelialization in diabetic wound. Consequently, the hydrogel patch based on β-glucans from Dictyophora indusiata appears to be an effective agent for promoting wound healing, thereby offering a novel therapeutic strategy for the repair of diabetic wound.
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Affiliation(s)
- Zeguo Feng
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Shan Lu
- Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, Institute of Design Science and Basic Components, Xi'an Jiaotong University, Xi'an 710049, China
| | - Ying He
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Yanting Sun
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Lei Ma
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Hui Guo
- Department of Endocrinology, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China
| | - Yiting Kang
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Rui Xu
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Yifei Song
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Lei Huang
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
| | - Liguo Qin
- Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, Institute of Design Science and Basic Components, Xi'an Jiaotong University, Xi'an 710049, China
| | - Jianbao Zhang
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
| | - Yali Zhang
- Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Health and Rehabilitation Science, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
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Mohebali F, Aghabarari B, Vaezi MR, Zarei Z, Hassanpour G, Alizadeh Z, Latifi A, Mohebali M. Biomacromolecule chitosan carrying meglumine antimoniate coated on a silver/polyurethane nanocomposite as a wound dressing: Therapeutic efficacy on cutaneous leishmaniasis caused by Leishmania major in BALB/c mice. Int J Biol Macromol 2025; 307:141847. [PMID: 40057056 DOI: 10.1016/j.ijbiomac.2025.141847] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/22/2024] [Revised: 03/03/2025] [Accepted: 03/06/2025] [Indexed: 03/16/2025]
Abstract
The high drug-carrying capacity and biocompatibility of chitosan (CS), a versatile biomacromolecule, have received special attention in recent years. This study focused on CS containing meglumine antimoniate (MA) for treating leishmaniasis, which was coated onto a silver/polyurethane (Ag.MA.CS/PUF). The newly synthesized nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR), inductively coupled plasma (ICP), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) surface area analysis, field emission scanning electron microscope/energy dispersive spectroscopy (FESEM/EDS), and transmission electron microscopy (TEM). To confirm the in vivo results, we administered the Ag.MA.CS/PUF nanocomposite topically to skin lesions caused by L. major (MRHO/IR/75/ER) in 56 inbred BALB/c mice in intervention (n = 42) and control (n = 14) groups, once daily for four weeks. Skin lesion sizes and amastigote counts were measured before treatment and four weeks post-treatment. At these intervals, the average size of skin lesions in the Ag.MA.CS/PUF group decreased by 28 %, from 3.02 ± 0.98 to 2.17 ± 0.33 mm2. In contrast, the average size of lesions in the negative control group significantly increased from 3.58 ± 2.05 to 8.73 ± 5.15 mm2 (p < 0.05). Furthermore, the parasite load in the Ag.MA.CS/PUF nanocomposite group was significantly reduced by 80 % compared to the negative control group (p = 0.001). These findings suggest promising prospects for improving treatment outcomes in the future.
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Affiliation(s)
- Fatemeh Mohebali
- Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Karaj, Iran
| | - Behzad Aghabarari
- Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Karaj, Iran.
| | - Mohammad Reza Vaezi
- Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Karaj, Iran
| | - Zabihollah Zarei
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Meshkin Shahr Research Station, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Gholamreza Hassanpour
- Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
| | - Zahra Alizadeh
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Alireza Latifi
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| | - Mehdi Mohebali
- Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
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Silva R, Medeiros M, Paula CTB, Saraiva S, Rebelo RC, Pereira P, Coelho JFJ, Serra AC, Fonseca AC. Light-Mediated 3D-Printed Wound Dressings Based on Natural Polymers with Improved Adhesion and Antioxidant Properties. Polymers (Basel) 2025; 17:1114. [PMID: 40284379 PMCID: PMC12031436 DOI: 10.3390/polym17081114] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2025] [Revised: 04/17/2025] [Accepted: 04/18/2025] [Indexed: 04/29/2025] Open
Abstract
The lack of personalized wound dressings tailored to individual needs can significantly hinder wound healing. Hydrogels offer a promising solution, as they can be engineered to mimic the extracellular matrix (ECM), providing an optimal environment for wound repair. The integration of digital light processing (DLP), a high-resolution 3D printing process, allows precise customization of hydrogel-based wound dressings. In this study, gelatin methacrylate (GelMA)-based formulations were prepared in combination with three different polymeric precursors: methacrylated hyaluronic acid (HAMA), poly (ethylene glycol) diacrylate (PEGDA) and allyl cellulose (MCCA). These precursors were used to print high-resolution micropatterned patches. The printed constructs revealed a high gel content and a good resistance to hydrolytic degradation. To improve the adhesive and antioxidant properties of the printed patches, gallic acid (GA) was incorporated through surface functionalization. This enabled the scavenging of approximately 80% of free radicals within just 4 h. The adhesive properties of the printed wound dressings were also significantly improved, with further enhancement observed upon the addition of Fe3+ ions. In vitro cytocompatibility tests using a fibroblast (NHDF) cell line confirmed the suitability of the materials for biomedical applications. Thus, this study demonstrates the potential of DLP-printed hydrogels as advanced personalized wound dressing materials.
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Affiliation(s)
- Rute Silva
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
| | - Matilde Medeiros
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
| | - Carlos T. B. Paula
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
- IPN, Instituto Pedro Nunes, Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, Rua Pedro Nunes, 3030-199 Coimbra, Portugal
| | - Sofia Saraiva
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
| | - Rafael C. Rebelo
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
| | - Patrícia Pereira
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
- IPN, Instituto Pedro Nunes, Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, Rua Pedro Nunes, 3030-199 Coimbra, Portugal
| | - Jorge F. J. Coelho
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
- IPN, Instituto Pedro Nunes, Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, Rua Pedro Nunes, 3030-199 Coimbra, Portugal
| | - Arménio C. Serra
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
| | - Ana C. Fonseca
- CEMMPRE, ARISE, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima-Polo II, 3030-790 Coimbra, Portugal; (R.S.); (M.M.); (S.S.); (R.C.R.); (P.P.); (J.F.J.C.); (A.C.S.)
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Vlasova TI, Brodovskaya EP, Madonov KS, Ageev VP, Abelova AP, Pinyaev SI, Vlasov AP. Regenerative Potential of Platelet-Rich Plasma in Diabetes Mellitus. DOKLADY BIOLOGICAL SCIENCES : PROCEEDINGS OF THE ACADEMY OF SCIENCES OF THE USSR, BIOLOGICAL SCIENCES SECTIONS 2025; 521:123-133. [PMID: 40216681 DOI: 10.1134/s0012496624600556] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/15/2024] [Revised: 01/15/2025] [Accepted: 01/20/2025] [Indexed: 05/07/2025]
Abstract
The regenerative potential of platelet-rich plasma (PRP) samples from patients with type 1 diabetes mellitus (DM) was studied in vitro. In a series of experiments, PRP was added to the culture medium of human dermal fibroblasts. The cells were tested for metabolic (including reactive oxygen species production), migration, and proliferative activities at several time points; growth factors and exosome levels were assessed in PRP and the medium. A lower proliferative effect, pro-oxidant properties, and toxicity were observed for PRPs from diabetic patients. Namely, treated fibroblasts showed lower metabolic activity, a lower cell viability, and a greater percentage of necrosis in culture, while their migration properties were not impaired. PRPs from diabetic patients exerted a lower stimulatory effect on growth factor secretion by dermal fibroblasts. PRPs from elderly diabetics had the greatest effects.
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Affiliation(s)
- T I Vlasova
- Ogarev National Research Mordovian State University, Saransk, Russia.
| | - E P Brodovskaya
- Ogarev National Research Mordovian State University, Saransk, Russia
| | - K S Madonov
- Ogarev National Research Mordovian State University, Saransk, Russia
| | - V P Ageev
- Ogarev National Research Mordovian State University, Saransk, Russia
| | - A P Abelova
- Ogarev National Research Mordovian State University, Saransk, Russia
| | - S I Pinyaev
- Ogarev National Research Mordovian State University, Saransk, Russia
| | - A P Vlasov
- Ogarev National Research Mordovian State University, Saransk, Russia
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de Figueiredo AC, Teixeira BN, Anaya-Mancipe JM, Cardoso VS, Vermelho AB, da Silva Moreira Thiré RM. Sub-Micrometer Polycaprolactone and Polyethylene Glycol-Based Fiber Mats With Iodine and Its Potential for Wound Healing. J Biomed Mater Res A 2025; 113:e37913. [PMID: 40200792 DOI: 10.1002/jbm.a.37913] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/10/2025] [Revised: 03/06/2025] [Accepted: 04/01/2025] [Indexed: 04/10/2025]
Abstract
This study introduces an innovative approach to developing advanced antimicrobial wound dressings by engineering fiber mats of polycaprolactone (PCL) and polycaprolactone/polyethylene glycol (PCL/PEG) loaded with iodine using solution blow spinning (SBS). The mats exhibited a unique morphology, combining fibers and beads, with average fiber diameters of 570 nm for 12% (w/v) PCL and 470 nm for 1% (w/v) PEG in 12% (w/v) PCL, and bead diameters of 11.34 μm and 10.43 μm, respectively. PEG incorporation rendered the mats hydrophilic and significantly enhanced their swelling capacity, which is essential for wound exudate management. Remarkably, iodine was incorporated at a concentration specifically optimized to achieve its minimum inhibitory concentration (MIC) against a range of microorganisms while preserving the structural integrity and release profile of the mats. While PEG facilitated a higher initial iodine release rate, equilibrium was achieved after 5 days. UV-Vis spectroscopy unveiled the formation of transient iodine complexes with both PCL and PEG, underscoring their functional synergy. Biological assays revealed that PCL/PEG mats loaded with 4.26 mg/mL of iodine (PCL/PEG 3MIC) exhibited potent antimicrobial efficacy against gram-positive and gram-negative bacteria, as well as fungi, alongside significant in vitro wound healing potential. These findings underscore the promise of PCL/PEG iodine-loaded mats as an innovative platform for next-generation antimicrobial wound care, combining effective infection control with enhanced wound healing capabilities.
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Affiliation(s)
- Agnes Chacor de Figueiredo
- COPPE/Program of Metallurgical and Materials Engineering - PEMM, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
| | - Bruna Nunes Teixeira
- COPPE/Program of Metallurgical and Materials Engineering - PEMM, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
| | - Javier Mauricio Anaya-Mancipe
- COPPE/Program of Metallurgical and Materials Engineering - PEMM, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
| | - Verônica Silva Cardoso
- Institute of Microbiology Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
| | - Alane Beatriz Vermelho
- Institute of Microbiology Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
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9
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Johnson CR, Schoenfisch MH. Photoinitiated Nitric Oxide Release as an Antibacterial Treatment for Chronic Wounds. ACS Biomater Sci Eng 2025; 11:1510-1522. [PMID: 39933146 DOI: 10.1021/acsbiomaterials.4c01955] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/13/2025]
Abstract
Taking advantage of their innate roles as antibacterial strategies, the dual activity of photobiomodulation (PBM) and nitric oxide (NO) was combined to provide a tunable, on-demand chronic wound therapeutic. S-nitrosothiol-modified mesoporous silica nanoparticles (RSNO-MSNs) were doped into polyurethane (PU) to demonstrate preliminary utility as an antibacterial wound dressing treatment for chronic wounds. Photoinitiated and resultant NO-release kinetics and payloads were evaluated at 405, 430, and 530 nm for multiple irradiances. The use of photons and the NO-releasing MSNs against common chronic wound pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, proved to be highly bactericidal. Cytocompatibility of the treatment was confirmed using human epidermal keratinocytes, a representative skin cell line.
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Affiliation(s)
- Courtney R Johnson
- Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
| | - Mark H Schoenfisch
- Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
- Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
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10
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Szunerits S, Boukherroub R, Kleber C, Knoll W, Yunda J, Rumipamba J, Torres G, Melinte S. Biosensors integrated within wearable devices for monitoring chronic wound status. APL Bioeng 2025; 9:010901. [PMID: 39926013 PMCID: PMC11803754 DOI: 10.1063/5.0220516] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/26/2024] [Accepted: 01/06/2025] [Indexed: 02/11/2025] Open
Abstract
Slowly healing wounds significantly affect the life quality of patients in different ways, due to constant pain, unpleasant odor, reduced mobility up to social isolation, and personal frustration. While remote wound management has become more widely accepted since the COVID-19 pandemic, delayed treatment remains frequent and results in several wound healing related complications. As inappropriate management of notably diabetic foot ulcers is linked to a high risk of amputation, effective management of wounds in a patient-centered manner remains important to be implemented. The integration of diagnostic devices into wound bandages is under way, owing to advancements in materials science and nanofabrication strategies as well as innovation in communication technologies together with machine learning and data-driven assessment tools. Leveraging advanced analytical approaches around local pH, temperature, pressure, and wound biomarker sensing is expected to facilitate adequate wound treatment. The state-of-the-art of time-resolved monitoring of the wound status by quantifying key physiological parameters as well as wound biomarkers' concentration is presented herewith. A special focus will be given to smart bandages with on-demand delivery capabilities for improved wound management.
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Affiliation(s)
| | - Rabah Boukherroub
- Univ. Lille, CNRS, Univ. Polytechnique Hauts-de-France, UMR 8520—IEMN, F-59000 Lille, France
| | - Christoph Kleber
- Laboratory for Life Sciences and Technology (LiST), Faculty of Medicine and Dentistry, Danube Private University, 3500 Krems, Austria
| | - Wolfgang Knoll
- Laboratory for Life Sciences and Technology (LiST), Faculty of Medicine and Dentistry, Danube Private University, 3500 Krems, Austria
| | - Jhonny Yunda
- Université catholique de Louvain, ICTEAM, 1348 Louvain-la-Neuve, Belgium
| | - José Rumipamba
- Université catholique de Louvain, ICTEAM, 1348 Louvain-la-Neuve, Belgium
| | - Guido Torres
- Université catholique de Louvain, ICTEAM, 1348 Louvain-la-Neuve, Belgium
| | - Sorin Melinte
- Université catholique de Louvain, ICTEAM, 1348 Louvain-la-Neuve, Belgium
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11
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Kłapcia A, Domalik-Pyzik P. Hydrogel Dressings as Insulin Delivery Systems for Diabetic Wounds. Front Biosci (Elite Ed) 2025; 17:26446. [PMID: 40150982 DOI: 10.31083/fbe26446] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/05/2024] [Revised: 10/29/2024] [Accepted: 11/08/2024] [Indexed: 03/29/2025]
Abstract
Diabetic wounds are one of the most common and challenging complications of diabetes. Similar to chronic wounds, diabetic wounds are difficult to treat due to prolonged inflammation, a lack of angiogenesis, abnormal differentiation of new scar tissue, and the occurrence of numerous bacterial infections. Moreover, elevated sugar levels in tissues disrupt the healing process by enhancing inflammatory reactions, disrupting signaling pathways, and leading to the production of abnormal biological structures, which contribute to improper cell differentiation. Traditional dressings, such as bandages, gauze, and semi-occlusive foams, are inadequate for diabetic wounds with high exudation; moreover, frequently changing the dressing can cause secondary irritation. Hence, innovative hydrogel dressings are being developed, which, thanks to their soft polymer matrix, provide an ideal substrate for regenerating tissue. Hydrogels also allow for the introduction and controlled release of growth factors, making them a promising solution for treating diabetic wounds. Recently, researchers have focused on insulin, a hormone secreted by the human body to lower blood sugar levels, due to its interesting characteristics, such as supporting anti-inflammatory and proangiogenic processes and stimulating cell migration and proper proliferation. This review discusses the most important aspects of diabetes and diabetic wounds and traditional and innovative treatment methods, particularly hydrogel dressings used as systems for insulin delivery in response to glucose concentration.
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Affiliation(s)
- Agnieszka Kłapcia
- Academic Centre for Materials and Nanotechnology, AGH University of Krakow, 30-059 Krakow, Poland
| | - Patrycja Domalik-Pyzik
- Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30-059 Krakow, Poland
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12
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Sk MS, Mwangomo R, Daniel L, Gilmore J. Solution Blow Spinning: An Emerging Nanomaterials-Based Wound-Care Technology. J Biomed Mater Res B Appl Biomater 2025; 113:e35513. [PMID: 39854136 DOI: 10.1002/jbm.b.35513] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/27/2024] [Revised: 09/09/2024] [Accepted: 11/10/2024] [Indexed: 01/26/2025]
Abstract
Application of one-dimensional nanofibers have witnessed exponential growth over the past few decades and are still emerging with their excellent physicochemical and electrical properties. The driving force behind this intriguing transition lies in their unique high surface-to-volume ratio, ubiquitous nanodomains, improved tensile strength, and flexibility to incorporate deliberate functionalities required for specific and advanced applications. Besides numerous benefits, nanomaterials may adversely interact with biological tissues and potentially be cytotoxic and carcinogenic. However, precisely engineered design can outperform the risk with myriad benefits. Wound care technologies are evolving, and products involved in wound care management have a yearly market value of $15-22 billion. Solution blow spinning (SBS) is a facile technique to synthesize biocompatible nanofibers with scalable processing variables for multidirectional biomedical applications. SBS is feasible for a wide range of thermoplastic polymers and nanomaterials to fabricate nanocomposites. This review will focus on the relevance of SBS technology for wound care, including dressings, drug delivery, tissue engineering scaffolds, and sensors.
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Affiliation(s)
- Md Salauddin Sk
- Department of Bioengineering, Clemson University, Clemson, South Carolina, USA
| | - Ruth Mwangomo
- Department of Bioengineering, Clemson University, Clemson, South Carolina, USA
| | - Luke Daniel
- Department of Bioengineering, Clemson University, Clemson, South Carolina, USA
| | - Jordon Gilmore
- Department of Bioengineering, Clemson University, Clemson, South Carolina, USA
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13
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Neuhoferova E, Kindermann M, Buzgo M, Vocetkova K, Panek D, Cigler P, Benson V. Topical siRNA therapy of diabetic-like wound healing. J Mater Chem B 2025; 13:1037-1051. [PMID: 39630486 DOI: 10.1039/d4tb01547a] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/16/2025]
Abstract
Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem - polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.
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Affiliation(s)
- Eva Neuhoferova
- Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czechia.
- Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czechia
| | - Marek Kindermann
- Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 166 10, Prague 6, Czechia.
- Department of Physical Chemistry, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czechia
| | - Matej Buzgo
- Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague, Czechia
- InoCure s.r.o., Politickych veznu 13, 100 00, Prague, Czechia
| | - Karolina Vocetkova
- Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague, Czechia
| | - Dalibor Panek
- Faculty of Biomedical Engineering, Czech Technical University in Prague, Namesti Sitna 3105, Kladno 2, 272 01, Czechia
| | - Petr Cigler
- Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 166 10, Prague 6, Czechia.
| | - Veronika Benson
- Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czechia.
- Technical University of Liberec, Faculty of Health Studies, Trebizskeho 1402, 46001, Liberec, Czechia
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14
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Rajput JH, Rathi V, Mukherjee A, Yadav P, Gupta T, Das B, Poundarik A. A novel polyurethane-based silver foam dressing with superior antimicrobial action for management of infected chronic wounds. Biomed Mater 2024; 20:015005. [PMID: 39509820 DOI: 10.1088/1748-605x/ad8fe8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/23/2024] [Accepted: 11/07/2024] [Indexed: 11/15/2024]
Abstract
Wound healing is a complex and dynamic process supported by several cellular events. Around 13 million individuals globally suffer from chronic wounds yearly, for which dressings with excellent antimicrobial activity and cell viability (>70%, as per ISO 10993) are needed. Excessive use of silver can cause cytotoxicity and has been linked to increasing antimicrobial resistance. In this study, HDI Ag foam was synthesized using a safer hexamethylene diisocyanate-based prepolymer (HDI prepolymer) instead of commonly used diisocyanates like TDI and MDI and substantially lower Ag content than that incorporated in other Ag foams. In vitro characteristics of the HDI Ag foam were evaluated in comparison with leading clinically used foam-based dressings. All dressings underwent a detailed characterization in accordance with industrially accepted BS EN 13726 standards. The HDI Ag foam exhibited highest antimicrobial efficiency againstS. aureusandP. aeruginosa(static condition), with the lowest amount of Ag (0.2 wt%) on the wound contact surface. The extracts from HDI Ag foam showed superior cell viability (>70%), when tested on the L929 mouse fibroblast cell line. Measurements of moisture vapor transmission, fluid handling, physico-chemical and mechanical properties ensured that the HDI foam was clinically acceptable for chronic wound patients.
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Affiliation(s)
- Jay Hind Rajput
- Department of Metallurgical and Materials Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
| | - Varun Rathi
- Department of Biomedical Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
| | - Anwesha Mukherjee
- Department of Biomedical Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
| | - Pankaj Yadav
- Sheela Foam Ltd, Noida, Uttar Pradesh 201301, India
| | - Tarush Gupta
- Department of Plastic Surgery, Postgraduate Institute of Medical Education and Research, Chandigarh, Punjab 160012, India
| | - Bodhisatwa Das
- Department of Biomedical Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
| | - Atharva Poundarik
- Department of Metallurgical and Materials Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
- Department of Biomedical Engineering, Indian Institute of Technology Ropar, Ropar, Punjab 140001, India
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15
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Khattak S, Ullah I, Sohail M, Akbar MU, Rauf MA, Ullah S, Shen J, Xu H. Endogenous/exogenous stimuli‐responsive smart hydrogels for diabetic wound healing. AGGREGATE 2024. [DOI: 10.1002/agt2.688] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/06/2025]
Abstract
AbstractDiabetes significantly impairs the body's wound‐healing capabilities, leading to chronic, infection‐prone wounds. These wounds are characterized by hyperglycemia, inflammation, hypoxia, variable pH levels, increased matrix metalloproteinase activity, oxidative stress, and bacterial colonization. These complex conditions complicate effective wound management, prompting the development of advanced diabetic wound care strategies that exploit specific wound characteristics such as acidic pH, high glucose levels, and oxidative stress to trigger controlled drug release, thereby enhancing the therapeutic effects of the dressings. Among the solutions, hydrogels emerge as promising due to their stimuli‐responsive nature, making them highly effective for managing these wounds. The latest advancements in mono/multi‐stimuli‐responsive smart hydrogels showcase their superiority and potential as healthcare materials, as highlighted by relevant case studies. However, traditional wound dressings fall short of meeting the nuanced needs of these wounds, such as adjustable adhesion, easy removal, real‐time wound status monitoring, and dynamic drug release adjustment according to the wound's specific conditions. Responsive hydrogels represent a significant leap forward as advanced dressings proficient in sensing and responding to the wound environment, offering a more targeted approach to diabetic wound treatment. This review highlights recent advancements in smart hydrogels for wound dressing, monitoring, and drug delivery, emphasizing their role in improving diabetic wound healing. It addresses ongoing challenges and future directions, aiming to guide their clinical adoption.
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Affiliation(s)
- Saadullah Khattak
- The Fifth Affiliated Hospital of Wenzhou Medical University Lishui China
| | - Ihsan Ullah
- Zhejiang Engineering Research Center for Tissue Repair Materials Wenzhou Institute University of Chinese Academy of Sciences Wenzhou China
| | - Mohammad Sohail
- The Fifth Affiliated Hospital of Wenzhou Medical University Lishui China
| | - Muhammad Usman Akbar
- Oujiang Laboratory Key Laboratory of Alzheimer's Disease of Zhejiang Province Institute of Aging Wenzhou Medical University Wenzhou China
| | - Mohd Ahmar Rauf
- Department of Internal Medicine, Heme Oncology Unit, University of Michigan Ann Arbor Michigan USA
| | - Salim Ullah
- The Fifth Affiliated Hospital of Wenzhou Medical University Lishui China
| | - Jianliang Shen
- National Engineering Research Center of Ophthalmology and Optometry Eye Hospital Wenzhou Medical University Wenzhou China
- Wenzhou Institute University of Chinese Academy of Sciences Wenzhou China
| | - Hong‐Tao Xu
- The Fifth Affiliated Hospital of Wenzhou Medical University Lishui China
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16
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Basu P, Banerjee A, Okoro PD, Masoumi A, Kanjilal B, Akbari M, Martins‐Green M, Armstrong DG, Noshadi I. Integration of Functional Polymers and Biosensors to Enhance Wound Healing. Adv Healthc Mater 2024; 13:e2401461. [PMID: 39235365 PMCID: PMC11582501 DOI: 10.1002/adhm.202401461] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2024] [Revised: 07/17/2024] [Indexed: 09/06/2024]
Abstract
Biosensors have led to breakthroughs in the treatment of chronic wounds. Since the discovery of the oxygen electrode by Clarke, biosensors have evolved into the design of smart bandages that dispense drugs to treat wounds in response to physiological factors, such as pH or glucose concentration, which indicate pathogenic tendencies. Aptamer-based biosensors have helped identify and characterize pathogenic bacteria in wounds that often form antibiotic-resistant biofilms. Several functional polymers have served as indispensable parts of the fabrication of these biosensors. Beginning with natural polymers such as alginate, chitosan, and silk-based fibroin, which are biodegradable and absorptive, advances have been made in formulating biocompatible synthetic polymers such as polyurethane and polyethylene glycol designed to reduce non-specific binding of proteins and cells, making biosensors less painful or cumbersome for patient use. Recently, polycaprolactone has been developed, which offers ductility and a large surface-area-to-volume ratio. There is still room for advances in the fabrication and use of biosensors for wound healing and in this review, the trend in developing biosensors from biomarker detection to smart dressings to the incorporation of machine learning in designing customized wound patches while making application easier is highlighted and can be used for a long time.
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Affiliation(s)
- Proma Basu
- Department of BioengineeringUniversity of California, RiversideRiversideCA92521USA
| | - Aihik Banerjee
- Department of BioengineeringUniversity of California, RiversideRiversideCA92521USA
| | - Prince David Okoro
- Department of BioengineeringUniversity of California, RiversideRiversideCA92521USA
| | | | - Baishali Kanjilal
- Department of BioengineeringUniversity of California, RiversideRiversideCA92521USA
| | - Mohsen Akbari
- Department of Mechanical EngineeringUniversity of VictoriaVictoriaBCV8P 5C2Canada
| | - Manuela Martins‐Green
- Department of Molecular Cellular and Systems BiologyUniversity of California, RiversideRiversideCA92521USA
| | - David G. Armstrong
- Keck School of Medicine of the University of Southern CaliforniaLos AngelesCA90033USA
| | - Iman Noshadi
- Department of BioengineeringUniversity of California, RiversideRiversideCA92521USA
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17
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Astaneh ME, Fereydouni N. Advancing diabetic wound care: The role of copper-containing hydrogels. Heliyon 2024; 10:e38481. [PMID: 39640763 PMCID: PMC11619988 DOI: 10.1016/j.heliyon.2024.e38481] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/20/2024] [Revised: 09/24/2024] [Accepted: 09/25/2024] [Indexed: 12/07/2024] Open
Abstract
Diabetic wounds pose a significant challenge in healthcare due to their complex nature and the difficulties they present in treatment and healing. Impaired healing processes in individuals with diabetes can lead to complications and prolonged recovery times. However, recent advancements in wound healing provide reasons for optimism. Researchers are actively developing innovative strategies and therapies specifically tailored to address the unique challenges of diabetic wounds. One focus area is biomimetic hydrogel scaffolds that mimic the natural extracellular matrix, promoting angiogenesis, collagen deposition, and the healing process while also reducing infection risk. Copper nanoparticles and copper compounds incorporated into hydrogels release copper ions with antimicrobial, anti-inflammatory, and angiogenic properties. Copper reduces infection risk, modulates inflammatory response, and promotes tissue regeneration through cell adhesion, proliferation, and differentiation. Utilizing copper nanoparticles has transformative potential for expediting diabetic wound healing and improving patient outcomes while enhancing overall well-being by preventing severe complications associated with untreated wounds. It is crucial to write a review highlighting the importance of investigating the use of copper nanoparticles and compounds in diabetic wound healing and tissue engineering. These groundbreaking strategies hold the potential to transform the treatment of diabetic wounds, accelerating the healing process and enhancing patient outcomes.
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Affiliation(s)
- Mohammad Ebrahim Astaneh
- Department of Anatomical Sciences, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
- Department of Tissue Engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
- Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
| | - Narges Fereydouni
- Department of Tissue Engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
- Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
- Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
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18
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Cui Z, Chen Y, Song S, Wang J, Wei Y, Wu X, Zhao G. A carrier-free, injectable, and self-assembling hydrogel based on carvacrol and glycyrrhizin exhibits high antibacterial activity and enhances healing of MRSA-infected wounds. Colloids Surf B Biointerfaces 2024; 241:114068. [PMID: 38954940 DOI: 10.1016/j.colsurfb.2024.114068] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/23/2024] [Revised: 06/19/2024] [Accepted: 06/27/2024] [Indexed: 07/04/2024]
Abstract
Inspired by glycyrrhizin's strong pharmacological activities and the directed self-assembly into hydrogels, we created a novel carrier-free, injectable hydrogel (CAR@glycygel) by combining glycyrrhizin with carvacrol (CAR), without any other chemical crosslinkers, to promote wound healing on bacteria-infected skin. CAR appeared to readily dissolve and load into CAR@glycygel. CAR@glycygel had a dense, porous, sponge structure and strong antioxidant characteristics. In vitro, it showed better antibacterial ability than free CAR. For methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, and Escherichia coli, the diameter of inhibition zone values of CAR@glycygel were 3.80 ± 0.04, 3.31 ± 0.20 and 3.12 ± 0.24 times greater, respectively, than those of free CAR. The MICs for CAR@glycygel was 156.25 μg/mL while it was 1250.00 μg/mL for free CAR to these three bacteria. Its antibacterial mechanism appeared to involve destruction of the integrity of the bacterial cell wall and biomembrane, leading to a leakage of AKP and inhibition of biofilm formation. In vivo, CAR@glycygel effectively stopped bleeding. When applied to skin wounds on rats infected with MRSA, CAR@glycygel had strong bactericidal activity and improved wound healing. The wound healing rates for CAR@glycygel were 49.59 ± 15.78 %, 93.02 ± 3.09 % and 99.02 ± 0.55 % on day 3, day 7, and day 11, respectively, which were much better than blank control and positive control groups. Mechanisms of CAR@glycygel accelerating wound healing involved facilitating epidermis remolding, promoting the growth of hair follicles, stimulating collagen deposition, mitigating inflammation, and promoting angiogenesis. Overall, CAR@glycygel showed great potential as wound dressing for infected skin wounds.
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Affiliation(s)
- Zhengwei Cui
- College of Chemical Engneering, Qingdao University of Science and Technology, Qingdao, China
| | - Yunlai Chen
- College of Chemical Engneering, Qingdao University of Science and Technology, Qingdao, China
| | - Shiping Song
- China Animal Health and Epidemiology Center, Qingdao, China
| | - Junwei Wang
- China Animal Health and Epidemiology Center, Qingdao, China
| | - Yanjun Wei
- College of Chemical Engneering, Qingdao University of Science and Technology, Qingdao, China; Viwit Pharmaceutical Co., Ltd. Zaozhuang, Shandong, China
| | - Xianggen Wu
- College of Chemical Engneering, Qingdao University of Science and Technology, Qingdao, China; Viwit Pharmaceutical Co., Ltd. Zaozhuang, Shandong, China.
| | - Ge Zhao
- China Animal Health and Epidemiology Center, Qingdao, China.
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19
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Sanjarnia P, Picchio ML, Polegre Solis AN, Schuhladen K, Fliss PM, Politakos N, Metterhausen L, Calderón M, Osorio-Blanco ER. Bringing innovative wound care polymer materials to the market: Challenges, developments, and new trends. Adv Drug Deliv Rev 2024; 207:115217. [PMID: 38423362 DOI: 10.1016/j.addr.2024.115217] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/14/2023] [Revised: 01/24/2024] [Accepted: 02/18/2024] [Indexed: 03/02/2024]
Abstract
The development of innovative products for treating acute and chronic wounds has become a significant topic in healthcare, resulting in numerous products and innovations over time. The growing number of patients with comorbidities and chronic diseases, which may significantly alter, delay, or inhibit normal wound healing, has introduced considerable new challenges into the wound management scenario. Researchers in academia have quickly identified promising solutions, and many advanced wound healing materials have recently been designed; however, their successful translation to the market remains highly complex and unlikely without the contribution of industry experts. This review article condenses the main aspects of wound healing applications that will serve as a practical guide for researchers working in academia and industry devoted to designing, evaluating, validating, and translating polymer wound care materials to the market. The article highlights the current challenges in wound management, describes the state-of-the-art products already on the market and trending polymer materials, describes the regulation pathways for approval, discusses current wound healing models, and offers a perspective on new technologies that could soon reach consumers. We envision that this comprehensive review will significantly contribute to highlighting the importance of networking and exchanges between academia and healthcare companies. Only through the joint of these two actors, where innovation, manufacturing, regulatory insights, and financial resources act in harmony, can wound care products be developed efficiently to reach patients quickly and affordably.
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Affiliation(s)
- Pegah Sanjarnia
- POLYMAT, Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizábal, 3, 20018 Donostia-San Sebastián, Spain
| | - Matías L Picchio
- POLYMAT, Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizábal, 3, 20018 Donostia-San Sebastián, Spain; Instituto de Desarrollo Tecnológico para la Industria Química (INTEC), CONICET, Güemes 3450, Santa Fe 3000, Argentina
| | - Agustin N Polegre Solis
- Beiersdorf AG, Research & Development Department, Beiersdorfstraße 1-9, 22529 Hamburg, Germany
| | - Katharina Schuhladen
- Beiersdorf AG, Research & Development Department, Beiersdorfstraße 1-9, 22529 Hamburg, Germany
| | - Patricia M Fliss
- Beiersdorf AG, Research & Development Department, Beiersdorfstraße 1-9, 22529 Hamburg, Germany
| | - Nikolaos Politakos
- POLYMAT, Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizábal, 3, 20018 Donostia-San Sebastián, Spain
| | - Lutz Metterhausen
- Beiersdorf AG, Research & Development Department, Beiersdorfstraße 1-9, 22529 Hamburg, Germany
| | - Marcelo Calderón
- POLYMAT, Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizábal, 3, 20018 Donostia-San Sebastián, Spain; IKERBASQUE, Basque Foundation for Science, Plaza Euskadi 5, 48009 Bilbao, Spain
| | - Ernesto R Osorio-Blanco
- Beiersdorf AG, Research & Development Department, Beiersdorfstraße 1-9, 22529 Hamburg, Germany.
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20
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Jain N, Singh Y, Nouri A, Garg U, Pandey M. Assessment of healing capacity of glucose-responsive smart gels on the diabetic wound: A comprehensive review. J Drug Deliv Sci Technol 2024; 93:105403. [DOI: 10.1016/j.jddst.2024.105403] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2025]
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21
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Kaur D, Purwar R. Nanotechnological advancement in artificial intelligence for wound care. NANOTECHNOLOGICAL ASPECTS FOR NEXT-GENERATION WOUND MANAGEMENT 2024:281-318. [DOI: 10.1016/b978-0-323-99165-0.00005-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/04/2025]
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22
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Khaliq T, Sohail M, Minhas MU, Mahmood A, Munir A, Qalawlus AHM, Jabeen N, Kousar M, Anwar Z. Hyaluronic acid/alginate-based biomimetic hydrogel membranes for accelerated diabetic wound repair. Int J Pharm 2023; 643:123244. [PMID: 37463619 DOI: 10.1016/j.ijpharm.2023.123244] [Citation(s) in RCA: 15] [Impact Index Per Article: 7.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/27/2023] [Revised: 06/16/2023] [Accepted: 07/15/2023] [Indexed: 07/20/2023]
Abstract
The study aims to develop a new multifunctional biopolymer-based hydrogel membrane dressing by adopting a solvent casting method for the controlled release of cefotaxime sodium at the wound site. Sodium alginate enhances collagen production in the skin, which provides tensile strength to healing tissue. Moreover, the significance of extracellular molecules such as hyaluronic acid in the wound the healing cascade renders these biopolymers an essential ingredient for the fabrication of hydrogel membranes via physical crosslinking (hydrogen bonding). These membranes were further investigated in terms of their structure, and surface morphology, as well as cell viability analysis. A membrane with the most suitable characteristics was chosen as a candidate for cefotaxime sodium loading and in vivo analysis. Results show that the 3D porous nature of developed membranes allows optimum water vapor and oxygen transmission (>8.21 mg/mL) to divert excessive wound exudate away from the diabetic wound bed, MTT assay confirmed cell viability at more than 80%. In vivo results confirmed that the CTX-HA-Alg-PVA hydrogel group showed rapid wound healing with accelerated re-epithelization and a decreased inflammatory response. Conclusively, these findings indicate that CTX-HA-Alg-PVA hydrogel membranes exhibit a suitable niche for use as dressing membranes for healing of diabetic wounds.
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Affiliation(s)
- Touba Khaliq
- Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan
| | - Muhammad Sohail
- Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan; Faculty of Pharmacy, Cyprus International University, Nicosia 99258, Cyprus.
| | | | - Arshad Mahmood
- Collage of Pharmacy, Al Ain University, Abu Dhabi, United Arab Emirates; AU Health and Biomedical Research Center, Al Ain University, Abu Dhabi, United Arab Emirates
| | - Abubakar Munir
- Department of Pharmaceutical Sciences, The Superior University, Lahore 54600, Pakistan
| | | | - Nazish Jabeen
- Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan
| | - Mubeen Kousar
- Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan
| | - Zobia Anwar
- Govt. Postgraduate College Mandian, Abbottabad 22010, Pakistan
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23
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Chen Y, Wang X, Tao S, Wang Q, Ma PQ, Li ZB, Wu YL, Li DW. Research advances in smart responsive-hydrogel dressings with potential clinical diabetic wound healing properties. Mil Med Res 2023; 10:37. [PMID: 37608335 PMCID: PMC10463485 DOI: 10.1186/s40779-023-00473-9] [Citation(s) in RCA: 50] [Impact Index Per Article: 25.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/18/2023] [Accepted: 07/31/2023] [Indexed: 08/24/2023] Open
Abstract
The treatment of chronic and non-healing wounds in diabetic patients remains a major medical problem. Recent reports have shown that hydrogel wound dressings might be an effective strategy for treating diabetic wounds due to their excellent hydrophilicity, good drug-loading ability and sustained drug release properties. As a typical example, hyaluronic acid dressing (Healoderm) has been demonstrated in clinical trials to improve wound-healing efficiency and healing rates for diabetic foot ulcers. However, the drug release and degradation behavior of clinically-used hydrogel wound dressings cannot be adjusted according to the wound microenvironment. Due to the intricacy of diabetic wounds, antibiotics and other medications are frequently combined with hydrogel dressings in clinical practice, although these medications are easily hindered by the hostile environment. In this case, scientists have created responsive-hydrogel dressings based on the microenvironment features of diabetic wounds (such as high glucose and low pH) or combined with external stimuli (such as light or magnetic field) to achieve controllable drug release, gel degradation, and microenvironment improvements in order to overcome these clinical issues. These responsive-hydrogel dressings are anticipated to play a significant role in diabetic therapeutic wound dressings. Here, we review recent advances on responsive-hydrogel dressings towards diabetic wound healing, with focus on hydrogel structure design, the principle of responsiveness, and the behavior of degradation. Last but not least, the advantages and limitations of these responsive-hydrogels in clinical applications will also be discussed. We hope that this review will contribute to furthering progress on hydrogels as an improved dressing for diabetic wound healing and practical clinical application.
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Affiliation(s)
- Ying Chen
- Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, Fujian, China
| | - Xing Wang
- Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100090, China
- University of Chinese Academy of Sciences, Beijing, 100049, China
| | - Sheng Tao
- Senior Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100091, China
| | - Qi Wang
- Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, Fujian, China
| | - Pan-Qin Ma
- Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, Fujian, China
| | - Zi-Biao Li
- Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), Singapore, 138634, Singapore.
| | - Yun-Long Wu
- Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
| | - Da-Wei Li
- Senior Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, 100091, China.
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Khadem E, Kharaziha M, Salehi S. Colorimetric pH-responsive and hemostatic hydrogel-based bioadhesives containing functionalized silver nanoparticles. Mater Today Bio 2023; 20:100650. [PMID: 37206880 PMCID: PMC10189517 DOI: 10.1016/j.mtbio.2023.100650] [Citation(s) in RCA: 7] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/14/2022] [Revised: 04/27/2023] [Accepted: 04/29/2023] [Indexed: 05/21/2023] Open
Abstract
Here we develop and characterize a dual-cross-linked pH-responsive hydrogel based on the carboxyethyl chitosan-oxidized sodium alginate (CAO) containing silver nanoparticles (Ag NPs) functionalized with tannic acid/red cabbage (ATR). This hybrid hydrogel is formed via covalent and non-covalent cross-linking. The adhesive strength measured in contact with cow skin and compression strength is measured more than 3 times higher than that of CAO. Importantly, the incorporation of 1 wt% ATR into CAO significantly enhances the compression strength of CAO from 35.1 ± 2.1 kPa to 97.5 ± 2.9 kPa. Moreover, the cyclic compression tests confirm significantly higher elastic behavior of CAO after the addition of ATR-functionalized NPs to CAO. The CAO/ATR hydrogel is pH-sensitive and indicated remarkable color changes in different buffer solutions. The CAO/ATR also shows improved hemostatic properties and reduced clotting time compared to the clotting time of blood in contact with CAO hydrogel. In addition, while CAO/ATR is effective in inhibiting the growth of both Gram-positive and Gram-negative bacteria, CAO is only effective in inhibiting the growth of Gram-positive bacteria. Finally, the CAO/ATR hydrogel is cytocompatible with L929 fibroblasts. In summary, the resulting CAO/ATR hydrogel shows promising results in designing and constructing smart wound bioadhesives with high cytocompatibility, antibacterial properties, blood coagulation ability, and fast self-healing properties.
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Affiliation(s)
- Elham Khadem
- Department of Materials Engineering, Isfahan University of Technology, 84156-83111, Isfahan, Iran
| | - Mahshid Kharaziha
- Department of Materials Engineering, Isfahan University of Technology, 84156-83111, Isfahan, Iran
- Department of Biomaterials, University of Bayreuth, 95447, Bayreuth, Germany
- Corresponding author. Department of Materials Engineering, Isfahan University of Technology, 84156-83111, Isfahan, Iran.
| | - Sahar Salehi
- Department of Biomaterials, University of Bayreuth, 95447, Bayreuth, Germany
- Corresponding author.
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Biopolymers in diabetic wound care management: a potential substitute to traditional dressings. Eur Polym J 2023. [DOI: 10.1016/j.eurpolymj.2023.111979] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/09/2023]
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Keni R, Begum F, Gourishetti K, Viswanatha GL, Nayak PG, Nandakumar K, Shenoy RR. Diabetic wound healing approaches: an update. J Basic Clin Physiol Pharmacol 2023; 34:137-150. [PMID: 34995024 DOI: 10.1515/jbcpp-2021-0340] [Citation(s) in RCA: 15] [Impact Index Per Article: 7.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2021] [Accepted: 12/19/2021] [Indexed: 01/01/2023]
Abstract
Diabetic wounds are of profound clinical importance. Despite immense efforts directed towards its management, it results in the development of amputations, following a diagnosis of diabetic foot. With a better understanding of the complexities of the microbalance involved in the healing process, researchers have developed advanced methods for the management of wounds as well as diagnostic tools (especially, for wound infections) to be delivered to clinics sooner. In this review, we address the newer developments that hope to drive the transition from bench to bedside in the coming decade.
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Affiliation(s)
- Raghuvir Keni
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
| | - Farmiza Begum
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
| | - Karthik Gourishetti
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
| | | | - Pawan Ganesh Nayak
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
| | - Krishnadas Nandakumar
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
| | - Rekha R Shenoy
- Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India
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Ghafouri A, Jafari Karegar S, Hajiluian G, Hosseini S, Shidfar S, Kamalinejad M, Hosseini AF, Heydari I, Shidfar F. The effects of aqueous and ethanolic extracts of Rheum ribes on insulin-resistance and apolipoproteins in patients with type 2 diabetes mellitus: a randomized controlled trial. BMC Complement Med Ther 2023; 23:46. [PMID: 36788517 DOI: 10.1186/s12906-023-03878-0.pmid:] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/29/2022] [Accepted: 02/09/2023] [Indexed: 07/31/2024] Open
Abstract
BACKGROUND AND AIM Previous studies have shown that Rheum ribes (R. ribes) could be effective in controlling the blood glucose levels. This study was conducted to determine the effects of R. ribes supplementation on glycemic indices and apolipoproteins in patients with type 2 diabetes mellitus (T2DM). METHODS In the present randomized double-blind controlled trial, 60 type 2 diabetic patients aged 30-60 years with a body mass index (BMI) of 20-30 kg/m2 and hemoglobin A1c (HbA1c) of 6-8% were enrolled. Patients were randomly assigned to receive 450 mg of aqueous R. ribes extract (AG), 450 mg of ethanolic R. ribes extract (EG), or placebo (PG) three times daily for 6 weeks. At the baseline and at the end of the study, blood glucose levels, homeostatic model assessment of insulin resistance (HOMA-IR) and the homeostatic model assessment of β-cell dysfunction (HOMA-B), as well as apolipoprotein A-I (ApoA1) and apolipoprotein B (ApoB) were measured. RESULTS There was a significant decrease in the serum levels of insulin in AG and EG groups (P = 0.003 and P = 0.001, respectively), HOMA-IR (P = 0.01 and P = 0.001, respectively), HOMA-B (P = 0.002 and P = 0.001, respectively), ApoB (P = 0.006 and P = 0.03, respectively), ApoB/ApoA1 ratio (P = 0.016 and P = 0.04, respectively). However, a significant increase in ApoA1 (P = 0.08 and P = 0.05, respectively) with no significant changes in blood glucose, at the end of study compared to beginning values, were observed. None of the variables showed a significant change in PG. At the end of the study; while there were significant differences in insulin (P = 0.04), HOMA-IR (P = 0.03), HOMA-B (P = 0.01), ApoB (P = 0.02), and ApoB/ApoA1 ratio (P = 0.03) among the groups but ApoA1 had no significant change. CONCLUSION Consumption of R. ribes intake could have beneficial effects on insulin resistance and apolipoproteins in type 2 diabetic patients. (Registered at en.irct.ir, identification number: IRCT201410142709N31).
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Affiliation(s)
- Atieh Ghafouri
- Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Sahar Jafari Karegar
- Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
- Student Research Committee, Faculty of Public Health Branch, Iran University of Medical Sciences, Tehran, Iran
| | - Ghazaleh Hajiluian
- Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Sharieh Hosseini
- Department of Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | - Shahrzad Shidfar
- Worcester Memorial Hospital, University of Massachusetts, Worcester, MA, USA
| | - Mohammad Kamalinejad
- School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Agha Fatemeh Hosseini
- Department of Statistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Iraj Heydari
- Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
| | - Farzad Shidfar
- Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
- Research Center for Prevention of Cardiovascular Diseases, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
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Ghafouri A, Jafari Karegar S, Hajiluian G, Hosseini S, Shidfar S, Kamalinejad M, Hosseini AF, Heydari I, Shidfar F. The effects of aqueous and ethanolic extracts of Rheum ribes on insulin-resistance and apolipoproteins in patients with type 2 diabetes mellitus: a randomized controlled trial. BMC Complement Med Ther 2023; 23:46. [PMID: 36788517 PMCID: PMC9926739 DOI: 10.1186/s12906-023-03878-0] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/29/2022] [Accepted: 02/09/2023] [Indexed: 02/16/2023] Open
Abstract
BACKGROUND AND AIM Previous studies have shown that Rheum ribes (R. ribes) could be effective in controlling the blood glucose levels. This study was conducted to determine the effects of R. ribes supplementation on glycemic indices and apolipoproteins in patients with type 2 diabetes mellitus (T2DM). METHODS In the present randomized double-blind controlled trial, 60 type 2 diabetic patients aged 30-60 years with a body mass index (BMI) of 20-30 kg/m2 and hemoglobin A1c (HbA1c) of 6-8% were enrolled. Patients were randomly assigned to receive 450 mg of aqueous R. ribes extract (AG), 450 mg of ethanolic R. ribes extract (EG), or placebo (PG) three times daily for 6 weeks. At the baseline and at the end of the study, blood glucose levels, homeostatic model assessment of insulin resistance (HOMA-IR) and the homeostatic model assessment of β-cell dysfunction (HOMA-B), as well as apolipoprotein A-I (ApoA1) and apolipoprotein B (ApoB) were measured. RESULTS There was a significant decrease in the serum levels of insulin in AG and EG groups (P = 0.003 and P = 0.001, respectively), HOMA-IR (P = 0.01 and P = 0.001, respectively), HOMA-B (P = 0.002 and P = 0.001, respectively), ApoB (P = 0.006 and P = 0.03, respectively), ApoB/ApoA1 ratio (P = 0.016 and P = 0.04, respectively). However, a significant increase in ApoA1 (P = 0.08 and P = 0.05, respectively) with no significant changes in blood glucose, at the end of study compared to beginning values, were observed. None of the variables showed a significant change in PG. At the end of the study; while there were significant differences in insulin (P = 0.04), HOMA-IR (P = 0.03), HOMA-B (P = 0.01), ApoB (P = 0.02), and ApoB/ApoA1 ratio (P = 0.03) among the groups but ApoA1 had no significant change. CONCLUSION Consumption of R. ribes intake could have beneficial effects on insulin resistance and apolipoproteins in type 2 diabetic patients. (Registered at en.irct.ir, identification number: IRCT201410142709N31).
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Affiliation(s)
- Atieh Ghafouri
- grid.411746.10000 0004 4911 7066Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Sahar Jafari Karegar
- grid.411746.10000 0004 4911 7066Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran ,grid.411746.10000 0004 4911 7066Student Research Committee, Faculty of Public Health Branch, Iran University of Medical Sciences, Tehran, Iran
| | - Ghazaleh Hajiluian
- grid.411746.10000 0004 4911 7066Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Sharieh Hosseini
- grid.411463.50000 0001 0706 2472Department of Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | - Shahrzad Shidfar
- grid.168645.80000 0001 0742 0364Worcester Memorial Hospital, University of Massachusetts, Worcester, MA USA
| | - Mohammad Kamalinejad
- grid.411600.2School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Agha Fatemeh Hosseini
- grid.411746.10000 0004 4911 7066Department of Statistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
| | - Iraj Heydari
- grid.411746.10000 0004 4911 7066Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
| | - Farzad Shidfar
- Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. .,Research Center for Prevention of Cardiovascular Diseases, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
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29
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Recent progress in polymeric biomaterials and their potential applications in skin regeneration and wound care management. J Drug Deliv Sci Technol 2023. [DOI: 10.1016/j.jddst.2023.104319] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/06/2023]
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Zulbaran-Rojas A, Park C, El-Refaei N, Lepow B, Najafi B. Home-Based Electrical Stimulation to Accelerate Wound Healing-A Double-Blinded Randomized Control Trial. J Diabetes Sci Technol 2023; 17:15-24. [PMID: 34328024 PMCID: PMC9846397 DOI: 10.1177/19322968211035128] [Citation(s) in RCA: 16] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/01/2023]
Abstract
BACKGROUND Electrical stimulation (E-Stim) may offer a unique adjunctive treatment to heal complicated diabetic foot ulcers (DFU). Our primary goal is to examine the effectiveness of daily home-based E-Stim therapy to speed-up wound healing. METHODS Patients with chronic DFUs and mild to severe peripheral arterial disease (PAD) were recruited and randomized to either control (CG) or intervention (IG) groups. The IG received 1-hour home-based E-Stim therapy on daily basis for 4 weeks (4W). E-Stim was delivered through electrical pads placed above the ankle joint using a bio-electric stimulation technology (BEST®) platform (Tennant Biomodulator® PRO). The CG was provided with an identical but non-functional device for the same period. The primary outcome included wound area reduction at 4W from baseline (BL). RESULTS Thirty-eight patients were recruited and 5 were removed due to non-compliance or infection, leaving 33 participants (IG, n = 16; CG, n =17). At 4W, the IG showed a significant wound area reduction of 22% (BL: 7.4 ± 8.5 cm2 vs 4W: 5.8 ± 8.0 cm2, P = 0.002). Average of wound area was unchanged in the CG (P = 0.982). The self-report adherence to daily home-therapy was 93.9%. CONCLUSIONS Daily home-based E-Stim provides early results on the feasibility, acceptability, and effectiveness of E-Stim as an adjunctive therapy to speed up wound healings in patients with chronic DFU and mild to severe PAD.
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Affiliation(s)
- Alejandro Zulbaran-Rojas
- Michael E. DeBakey Department of
Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP),
Division of Vascular Surgery and Endovascular Therapy, Baylor College of Medicine,
Houston, TX, USA
| | - Catherine Park
- Michael E. DeBakey Department of
Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP),
Division of Vascular Surgery and Endovascular Therapy, Baylor College of Medicine,
Houston, TX, USA
| | - Nesreen El-Refaei
- Michael E. DeBakey Department of
Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP),
Division of Vascular Surgery and Endovascular Therapy, Baylor College of Medicine,
Houston, TX, USA
| | - Brian Lepow
- Michael E. DeBakey Department of
Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP),
Division of Vascular Surgery and Endovascular Therapy, Baylor College of Medicine,
Houston, TX, USA
| | - Bijan Najafi
- Michael E. DeBakey Department of
Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP),
Division of Vascular Surgery and Endovascular Therapy, Baylor College of Medicine,
Houston, TX, USA
- Bijan Najafi, PhD, MSc, Michael E. DeBakey
Department of Surgery, Interdisciplinary Consortium on Advanced Motion
Performance (iCAMP), Division of Vascular Surgery and Endovascular Therapy,
Baylor College of Medicine, 7200 Cambridge St., Houston, TX 77030, USA.
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Kumari P, Sharma S, Sharma PK, Alam A. Treatment Management of Diabetic Wounds Utilizing Herbalism: An Overview. Curr Diabetes Rev 2023; 19:92-108. [PMID: 35306989 DOI: 10.2174/1573399818666220318095320] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/17/2021] [Revised: 11/25/2021] [Accepted: 12/09/2021] [Indexed: 11/22/2022]
Abstract
BACKGROUND AND OBJECTIVES Diabetes Mellitus, commonly known as DM, is a metabolic disorder which is characterized by high blood glucose level, i.e., chronic hyperglycemia. If it is not managed properly, DM can lead to many severe complexities with time and can cause significant damage to the kidneys, heart, eyes, nerves and blood vessels. Diabetic foot ulcers (DFU) are one of those major complexities which affect around 15-25% of the population diagnosed with diabetes. Due to diabetic conditions, the body's natural healing process slows down leading to longer duration for healing of wounds only when taken care of properly. Herbal therapies are one of the approaches for the management and care of diabetic foot ulcer, which utilizes the concept of synergism for better treatment options. With the recent advancement in the field of nanotechnology and natural drug therapy, a lot of opportunities can be seen in combining both technologies and moving towards a more advanced drug delivery system to overcome the limitations of polyherbal formulations. METHODS During the writing of this document, the data was derived from existing original research papers gathered from a variety of sources such as PubMed, ScienceDirect, Google Scholar. CONCLUSION Hence, this review includes evidence about the current practices and future possibilities of nano-herbal formulation in treatment and management of diabetic wounds.
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Affiliation(s)
- Priya Kumari
- Department of Pharmacy, School of Medical and Allied Science, Galgotias University, 201310, India
| | - Shaweta Sharma
- Department of Pharmacy, School of Medical and Allied Science, Galgotias University, 201310, India
| | - Pramod Kumar Sharma
- Department of Pharmacy, School of Medical and Allied Science, Galgotias University, 201310, India
| | - Aftab Alam
- Department of Pharmacy, School of Medical and Allied Science, Galgotias University, 201310, India
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Singh SS, Behera SK, Rai S, Tripathy SK, Chakrabortty S, Mishra A. A critical review on nanomaterial based therapeutics for diabetic wound healing. Biotechnol Genet Eng Rev 2022:1-35. [PMID: 36576250 DOI: 10.1080/02648725.2022.2161732] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/04/2022] [Accepted: 12/16/2022] [Indexed: 12/29/2022]
Abstract
Diabetes mellitus is a chronic endocrine disease that occurs mostly in the state of hyperglycemia (elevated blood glucose level). In the recent times, diabetes is listed under world's utmost critical health issues. Wound treatment procedures are complicated in diabetic individuals all over the world. Diabetic wound care not only involves high-cost, but also the primary cause of hospitalization, which can lead to amputation thereby reducing diabetic patient life expectancy. To lower the risk of amputation, wound healing requires the development of effective treatments. Traditional management systems for Diabetes are frequently chastised due to their high costs, difficulties in maintaining a sustainable supply chain and limited disposal alternatives. The worrisome rise in diabetes prevalence has sparked a surge of interest in the discovery of viable remedies to supplement existing treatments. Nanomaterials wound healing has a lot of potential for treating and preventing wound infections and it has recently gained popularity owing to its ability to transport drugs to the wound area in a regulated fashion, potentially overpowering the limits of traditional approaches. This research assessed several nanosystems, such as nanocarriers and nanotherapeutics, to explore how they can benefit in diabetic wound healing, with a focus on current obstacles and future prospects.
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Affiliation(s)
- Swati Sucharita Singh
- School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
| | - Susanta Kumar Behera
- School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
- Department of cell biology, IMGENEX India Pvt. Ltd, Bhubaneswar, India
| | - Suchita Rai
- Bauxite -Alumina Division, Jawaharlal Nehru Aluminium Research Development and Design Centre, Nagpur, India
| | - Suraj K Tripathy
- School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
- School of Chemical Technology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
| | - Sankha Chakrabortty
- School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
- School of Chemical Technology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
| | - Amrita Mishra
- School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India
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3D Bioprinting Technology and Hydrogels Used in the Process. J Funct Biomater 2022; 13:jfb13040214. [PMID: 36412855 PMCID: PMC9680466 DOI: 10.3390/jfb13040214] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/22/2022] [Revised: 10/21/2022] [Accepted: 10/23/2022] [Indexed: 11/06/2022] Open
Abstract
3D bioprinting has gained visibility in regenerative medicine and tissue engineering due to its applicability. Over time, this technology has been optimized and adapted to ensure a better printability of bioinks and biomaterial inks, contributing to developing structures that mimic human anatomy. Therefore, cross-linked polymeric materials, such as hydrogels, have been highly targeted for the elaboration of bioinks, as they guarantee cell proliferation and adhesion. Thus, this short review offers a brief evolution of the 3D bioprinting technology and elucidates the main hydrogels used in the process.
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Polymer-Based Hydrogels Enriched with Essential Oils: A Promising Approach for the Treatment of Infected Wounds. Polymers (Basel) 2022; 14:polym14183772. [PMID: 36145917 PMCID: PMC9502037 DOI: 10.3390/polym14183772] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2022] [Revised: 09/01/2022] [Accepted: 09/05/2022] [Indexed: 11/16/2022] Open
Abstract
Among the factors that delay the wound healing process in chronic wounds, bacterial infections are a common cause of acute wounds becoming chronic. Various therapeutic agents, such as antibiotics, metallic nanoparticles, and essential oils have been employed to treat infected wounds and also prevent the wounds from bacterial invasion. Essential oils are promising therapeutic agents with excellent wound healing, anti-inflammatory and antimicrobial activities, and good soothing effects. Some essential oils become chemically unstable when exposed to light, heat, oxygen, and moisture. The stability and biological activity of essential oil can be preserved via loading into hydrogels. The polymer-based hydrogels loaded with bioactive agents are regarded as ideal wound dressings with unique features, such as controlled and sustained drug release mechanisms, good antibacterial activity, non-toxicity, excellent cytocompatibility, good porosity, moderate water vapour transmission rate, etc. This review addresses the pre-clinical outcomes of hydrogels loaded with essential oils in the treatment of infected wounds.
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Willer H, Spohn G, Morgenroth K, Thielemann C, Elvers-Hornung S, Bugert P, Delorme B, Giesen M, Schmitz-Rixen T, Seifried E, Pfarrer C, Schäfer R, Bieback K. Pooled human bone marrow-derived mesenchymal stromal cells with defined trophic factors cargo promote dermal wound healing in diabetic rats by improved vascularization and dynamic recruitment of M2-like macrophages. Front Immunol 2022; 13:976511. [PMID: 36059533 PMCID: PMC9437960 DOI: 10.3389/fimmu.2022.976511] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/23/2022] [Accepted: 07/27/2022] [Indexed: 11/13/2022] Open
Abstract
Human Mesenchymal Stromal Cells (hMSCs) are a promising source for cell-based therapies. Yet, transition to phase III and IV clinical trials is remarkably slow. To mitigate donor variabilities and to obtain robust and valid clinical data, we aimed first to develop a manufacturing concept balancing large-scale production of pooled hMSCs in a minimal expansion period, and second to test them for key manufacture and efficacy indicators in the clinically highly relevant indication wound healing. Our novel clinical-scale manufacturing concept is comprised of six single donor hMSCs master cell banks that are pooled to a working cell bank from which an extrapolated number of 70,000 clinical doses of 1x106 hMSCs/cm2 wound size can be manufactured within only three passages. The pooled hMSC batches showed high stability of key manufacture indicators such as morphology, immune phenotype, proliferation, scratch wound healing, chemotactic migration and angiogenic support. Repeated topical hMSCs administration significantly accelerated the wound healing in a diabetic rat model by delivering a defined growth factor cargo (specifically BDNF, EGF, G-CSF, HGF, IL-1α, IL-6, LIF, osteopontin, VEGF-A, FGF-2, TGF-β, PGE-2 and IDO after priming) at the specific stages of wound repair, namely inflammation, proliferation and remodeling. Specifically, the hMSCs mediated epidermal and dermal maturation and collagen formation, improved vascularization, and promoted cell infiltration. Kinetic analyses revealed transient presence of hMSCs until day (d)4, and the dynamic recruitment of macrophages infiltrating from the wound edges (d3) and basis (d9), eventually progressing to the apical wound on d11. In the wounds, the hMSCs mediated M2-like macrophage polarization starting at d4, peaking at d9 and then decreasing to d11. Our study establishes a standardized, scalable and pooled hMSC therapeutic, delivering a defined cargo of trophic factors, which is efficacious in diabetic wound healing by improving vascularization and dynamic recruitment of M2-like macrophages. This decision-making study now enables the validation of pooled hMSCs as treatment for impaired wound healing in large randomized clinical trials.
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Affiliation(s)
- Hélène Willer
- Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Mannheim, Germany
- Institute for Anatomy, University of Veterinary Medicine Hannover, Hannover, Germany
| | - Gabriele Spohn
- Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Service Baden-Württemberg-Hessen, Frankfurt am Main, Germany
| | - Kimberly Morgenroth
- Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Service Baden-Württemberg-Hessen, Frankfurt am Main, Germany
| | - Corinna Thielemann
- Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Mannheim, Germany
| | - Susanne Elvers-Hornung
- Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Mannheim, Germany
| | - Peter Bugert
- Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Mannheim, Germany
| | | | | | | | - Erhard Seifried
- Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Service Baden-Württemberg-Hessen, Frankfurt am Main, Germany
| | - Christiane Pfarrer
- Institute for Anatomy, University of Veterinary Medicine Hannover, Hannover, Germany
| | - Richard Schäfer
- Institute for Transfusion Medicine and Immunohaematology, German Red Cross Blood Service Baden-Württemberg-Hessen, Frankfurt am Main, Germany
- Institute for Transfusion Medicine and Gene Therapy, Medical Center - University of Freiburg, Freiburg, Germany
- Center for Chronic Immunodeficiency (CCI), Medical Center - University of Freiburg, Freiburg, Germany
| | - Karen Bieback
- Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Mannheim, Germany
- Mannheim Institute for Innate Immunoscience, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany
- FlowCore, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany
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Raja M, Lopes IC, Vadgama P. A preliminary electrochemical study of crosslinked albumin and collagen membranes as diffusion barriers for potentially degradable chronic wound biosensors. ELECTROCHEMICAL SCIENCE ADVANCES 2022. [DOI: 10.1002/elsa.202100132] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022] Open
Affiliation(s)
- Mohammad Raja
- School of Engineering and Materials Science Queen Mary University of London London UK
| | - Ilanna Campelo Lopes
- School of Engineering and Materials Science Queen Mary University of London London UK
- Departamento de Tecnologia Química Centro de Ciências Exatas e Tecnologia Universidade Federal do Maranhão Sao Luis Brazil
| | - Pankaj Vadgama
- School of Engineering and Materials Science Queen Mary University of London London UK
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Hagde P, Pingle P, Mourya A, Katta CB, Srivastava S, Sharma R, Singh KK, Sodhi RK, Madan J. Therapeutic potential of quercetin in diabetic foot ulcer: Mechanistic insight, challenges, nanotechnology driven strategies and future prospects. J Drug Deliv Sci Technol 2022. [DOI: 10.1016/j.jddst.2022.103575] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
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Adeleke O, Oboh G, Adefegha S, Osesusi A. Effect of aqueous extract from root and leaf of Sphenocentrum jollyanum pierre on wounds of diabetic rats: Influence on wound tissue cytokines, vascular endothelial growth factor and microbes. JOURNAL OF ETHNOPHARMACOLOGY 2022; 293:115266. [PMID: 35398496 DOI: 10.1016/j.jep.2022.115266] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/15/2021] [Revised: 03/12/2022] [Accepted: 04/04/2022] [Indexed: 06/14/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Sphenocentrum jollyanum is a flowering plant of the Menispermaceae family with bright yellow roots and wedged-shaped leaves. The plant is reputed to possess exceptional wound healing properties and used in folkloric medicine to dress chronic wounds. AIM OF THE STUDY Wound repair in a hyperglycemic state is known to be impaired and delayed making treatment a difficult challenge. This study sought how the aqueous extracts of root and leaf of Sphenocentrum jollyanum facilitated wound healing by modulating pro-inflammatory cytokines, vascular endothelial growth factor and microbial colonization on excision wound created in diabetic rats. METHODS Diabetes (blood glucose >250 mg/dl) was induced by feeding normal rats with high fat diet for 14 days after which intraperitoneal injection of low dose streptozotocin (35 mg/kg b.w.) was administered. Wounds were subsequently created and treatments administered afterwards for 14 days. RESULTS Administration of Sphenocentrum jollyanum root and leaf extracts both orally and topically (100 and 200 mg/kg b.w) significantly (p < 0.05) reduced secretion of pro-inflammatory cytokines (TNF-α, IL-6), number of microbial colonies (CFU/ml × 102), activity of myeloperoxidase and significantly increased growth factor secretion on wounds of the diabetic rats. Histological evaluations of wound tissues of treated diabetic rats revealed matured tissue granulation, presence of new blood vessels, collagen and fibroblast with fewer inflammatory cells. CONCLUSION The use of Sphenocentrum jollyanum effectively enhanced wound healing which may be related to constituents identified by GC-MS analysis and can thus, be suggested as a therapeutic agent for diabetic wound management.
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Affiliation(s)
- Oluwakemi Adeleke
- Functional Foods, Nutraceuticals and Phytomedicine Laboratory. Department of Biochemistry, Federal University of Technology Akure, Akure, Nigeria.
| | - Ganiyu Oboh
- Functional Foods, Nutraceuticals and Phytomedicine Laboratory. Department of Biochemistry, Federal University of Technology Akure, Akure, Nigeria
| | - Stephen Adefegha
- Functional Foods, Nutraceuticals and Phytomedicine Laboratory. Department of Biochemistry, Federal University of Technology Akure, Akure, Nigeria
| | - Adebayo Osesusi
- Department of Microbiology, Federal University of Technology Akure, Akure, Nigeria
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Awasthi A, Gulati M, Kumar B, Kaur J, Vishwas S, Khursheed R, Porwal O, Alam A, KR A, Corrie L, Kumar R, Kumar A, Kaushik M, Jha NK, Gupta PK, Chellappan DK, Gupta G, Dua K, Gupta S, Gundamaraju R, Rao PV, Singh SK. Recent Progress in Development of Dressings Used for Diabetic Wounds with Special Emphasis on Scaffolds. BIOMED RESEARCH INTERNATIONAL 2022; 2022:1659338. [PMID: 35832856 PMCID: PMC9273440 DOI: 10.1155/2022/1659338] [Citation(s) in RCA: 17] [Impact Index Per Article: 5.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/18/2021] [Revised: 05/19/2022] [Accepted: 06/08/2022] [Indexed: 11/17/2022]
Abstract
Diabetic wound (DW) is a secondary application of uncontrolled diabetes and affects about 42.2% of diabetics. If the disease is left untreated/uncontrolled, then it may further lead to amputation of organs. In recent years, huge research has been done in the area of wound dressing to have a better maintenance of DW. These include gauze, films, foams or, hydrocolloid-based dressings as well as polysaccharide- and polymer-based dressings. In recent years, scaffolds have played major role as biomaterial for wound dressing due to its tissue regeneration properties as well as fluid absorption capacity. These are three-dimensional polymeric structures formed from polymers that help in tissue rejuvenation. These offer a large surface area to volume ratio to allow cell adhesion and exudate absorbing capacity and antibacterial properties. They also offer a better retention as well as sustained release of drugs that are directly impregnated to the scaffolds or the ones that are loaded in nanocarriers that are impregnated onto scaffolds. The present review comprehensively describes the pathogenesis of DW, various dressings that are used so far for DW, the limitation of currently used wound dressings, role of scaffolds in topical delivery of drugs, materials used for scaffold fabrication, and application of various polymer-based scaffolds for treating DW.
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Affiliation(s)
- Ankit Awasthi
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Monica Gulati
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
- Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia
| | - Bimlesh Kumar
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Jaskiran Kaur
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Sukriti Vishwas
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Rubiya Khursheed
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Omji Porwal
- Department of Pharmacognosy, Faculty of Pharmacy, Tishk International University-Erbil, Kurdistan Region, Iraq
| | - Aftab Alam
- Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al Kharj, 11942 KSA, Saudi Arabia
| | - Arya KR
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Leander Corrie
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Rajan Kumar
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Ankit Kumar
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
| | - Monika Kaushik
- Amity Institute of Pharmacy, Amity University Madhya Pradesh, Gwalior, Madhya Pradesh 474001, India
| | - Niraj Kumar Jha
- Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Plot No. 32-34 Knowledge Park III, Greater Noida, Uttar Pradesh 201310, India
| | - Piyush Kumar Gupta
- Department of Life Sciences, School of Basic Sciences and Research, Sharda University, Plot No. 32-34, Knowledge Park III, Greater Noida, 201310 Uttar Pradesh, India
- Department of Biotechnology, Graphic Era Deemed to be University, Dehradun, 248002 Uttarakhand, India
| | - Dinesh Kumar Chellappan
- Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia
| | - Gaurav Gupta
- School of Pharmacy, Suresh Gyan Vihar University, Mahal Road, Jagatpura, Jaipur, India
- Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
- Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India
| | - Kamal Dua
- Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia
- Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, NSW 2007, Australia
| | - Saurabh Gupta
- Chitkara College of Pharmacy, Chitkara University, Punjab, India
| | - Rohit Gundamaraju
- ER Stress and Mucosal Immunology Lab, School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia 7248
| | - Pasupuleti Visweswara Rao
- Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, 88400 Sabah, Malaysia
- Centre for International Relations and Research Collaborations, Reva University, Rukmini Knowledge Park, Rukmini Knowledge Park, Kattigenahili, Yelahanka, Bangalore, 560064, , Karnataka, India
| | - Sachin Kumar Singh
- School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
- Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia
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40
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Bioactive Natural and Synthetic Polymers for Wound Repair. Macromol Res 2022. [DOI: 10.1007/s13233-022-0062-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/18/2022]
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41
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Weigelt MA, Lev-Tov HA, Tomic-Canic M, Lee WD, Williams R, Strasfeld D, Kirsner RS, Herman IM. Advanced Wound Diagnostics: Toward Transforming Wound Care into Precision Medicine. Adv Wound Care (New Rochelle) 2022; 11:330-359. [PMID: 34128387 PMCID: PMC8982127 DOI: 10.1089/wound.2020.1319] [Citation(s) in RCA: 23] [Impact Index Per Article: 7.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/08/2020] [Accepted: 05/29/2021] [Indexed: 11/01/2022] Open
Abstract
Significance: Nonhealing wounds are an ever-growing global pandemic, with mortality rates and management costs exceeding many common cancers. Although our understanding of the molecular and cellular factors driving wound healing continues to grow, standards for diagnosing and evaluating wounds remain largely subjective and experiential, whereas therapeutic strategies fail to consistently achieve closure and clinicians are challenged to deliver individualized care protocols. There is a need to apply precision medicine practices to wound care by developing evidence-based approaches, which are predictive, prescriptive, and personalized. Recent Advances: Recent developments in "advanced" wound diagnostics, namely biomarkers (proteases, acute phase reactants, volatile emissions, and more) and imaging systems (ultrasound, autofluorescence, spectral imaging, and optical coherence tomography), have begun to revolutionize our understanding of the molecular wound landscape and usher in a modern age of therapeutic strategies. Herein, biomarkers and imaging systems with the greatest evidence to support their potential clinical utility are reviewed. Critical Issues: Although many potential biomarkers have been identified and several imaging systems have been or are being developed, more high-quality randomized controlled trials are necessary to elucidate the currently questionable role that these tools are playing in altering healing dynamics or predicting wound closure within the clinical setting. Future Directions: The literature supports the need for the development of effective point-of-care wound assessment tools, such as a platform diagnostic array that is capable of measuring multiple biomarkers at once. These, along with advances in telemedicine, synthetic biology, and "smart" wearables, will pave the way for the transformation of wound care into a precision medicine. Clinical Trial Registration number: NCT03148977.
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Affiliation(s)
- Maximillian A. Weigelt
- Dr. Phillip Frost Department of Dermatology & Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
| | - Hadar A. Lev-Tov
- Dr. Phillip Frost Department of Dermatology & Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
| | - Marjana Tomic-Canic
- Dr. Phillip Frost Department of Dermatology & Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
| | - W. David Lee
- Precision Healing, Inc., Newton, Massachusetts, USA
| | | | | | - Robert S. Kirsner
- Dr. Phillip Frost Department of Dermatology & Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
| | - Ira M. Herman
- Precision Healing, Inc., Newton, Massachusetts, USA
- Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, USA
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42
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Deng X, Gould M, Ali MA. A review of current advancements for wound healing: Biomaterial applications and medical devices. J Biomed Mater Res B Appl Biomater 2022; 110:2542-2573. [PMID: 35579269 PMCID: PMC9544096 DOI: 10.1002/jbm.b.35086] [Citation(s) in RCA: 97] [Impact Index Per Article: 32.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2022] [Revised: 04/28/2022] [Accepted: 04/30/2022] [Indexed: 12/12/2022]
Abstract
Wound healing is a complex process that is critical in restoring the skin's barrier function. This process can be interrupted by numerous diseases resulting in chronic wounds that represent a major medical burden. Such wounds fail to follow the stages of healing and are often complicated by a pro‐inflammatory milieu attributed to increased proteinases, hypoxia, and bacterial accumulation. The comprehensive treatment of chronic wounds is still regarded as a significant unmet medical need due to the complex symptoms caused by the metabolic disorder of the wound microenvironment. As a result, several advanced medical devices, such as wound dressings, wearable wound monitors, negative pressure wound therapy devices, and surgical sutures, have been developed to correct the chronic wound environment and achieve skin tissue regeneration. Most medical devices encompass a wide range of products containing natural (e.g., chitosan, keratin, casein, collagen, hyaluronic acid, alginate, and silk fibroin) and synthetic (e.g., polyvinyl alcohol, polyethylene glycol, poly[lactic‐co‐glycolic acid], polycaprolactone, polylactic acid) polymers, as well as bioactive molecules (e.g., chemical drugs, silver, growth factors, stem cells, and plant compounds). This review addresses these medical devices with a focus on biomaterials and applications, aiming to deliver a critical theoretical reference for further research on chronic wound healing.
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Affiliation(s)
- Xiaoxuan Deng
- Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand
| | - Maree Gould
- Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand
| | - M Azam Ali
- Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand
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Qin K, Pereira RFP, Coradin T, de Zea Bermudez V, Fernandes FM. Biomimetic Silk Macroporous Materials for Drug Delivery Obtained via Ice-Templating. ACS APPLIED BIO MATERIALS 2022; 5:2556-2566. [PMID: 35537179 DOI: 10.1021/acsabm.2c00020] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/04/2023]
Abstract
Silk from Bombyx mori is one of the most exciting materials in nature. The apparently simple arrangement of its two major components─two parallel filaments of silk fibroin (SF) coated by a common sericin (SS) sheath─provides a combination of mechanical and surface properties that can protect the moth during its most vulnerable phase, the pupal stage. Here, we recapitulate the topology of native silk fibers but shape them into three-dimensional porous constructs using an unprecedented design strategy. We demonstrate, for the first time, the potential of these macroporous silk foams as dermal patches for wound protection and for the controlled delivery of Rifamycin (Rif), a model antibiotic. The method implies (i) removing SS from silk fibers; (ii) shaping SF solutions into macroporous foams via ice-templating; (iii) stabilizing the SF macroporous foam in a methanolic solution of Rif; and (iv) coating Rif-loaded SF foams with a SS sheath. The resulting SS@SF foams exhibit water wicking capacity and accommodate up to ∼20% deformation without detaching from a skin model. The antibacterial behavior of Rif-loaded SS@SF foams against Staphylococcus aureus on agar plates outperforms that of SF foams (>1 week and 4 days, respectively). The reassembly of natural materials as macroporous foams─illustrated here for the reconstruction of silk-based materials─can be extended to other multicomponent natural materials and may play an important role in applications where controlled release of molecules and fluid transport are pivotal.
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Affiliation(s)
- Kankan Qin
- Sorbonne Université, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France
| | - Rui F P Pereira
- Chemistry Center and Chemistry Department, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
| | - Thibaud Coradin
- Sorbonne Université, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France
| | - Verónica de Zea Bermudez
- Chemistry Department and CQ-VR, University of Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal
| | - Francisco M Fernandes
- Sorbonne Université, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France
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Wang C, Sani ES, Gao W. Wearable Bioelectronics for Chronic Wound Management. ADVANCED FUNCTIONAL MATERIALS 2022; 32:2111022. [PMID: 36186921 PMCID: PMC9518812 DOI: 10.1002/adfm.202111022] [Citation(s) in RCA: 57] [Impact Index Per Article: 19.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/01/2021] [Indexed: 05/05/2023]
Abstract
Chronic wounds are a major healthcare issue and can adversely affect the lives of millions of patients around the world. The current wound management strategies have limited clinical efficacy due to labor-intensive lab analysis requirements, need for clinicians' experiences, long-term and frequent interventions, limiting therapeutic efficiency and applicability. The growing field of flexible bioelectronics enables a great potential for personalized wound care owing to its advantages such as wearability, low-cost, and rapid and simple application. Herein, recent advances in the development of wearable bioelectronics for monitoring and management of chronic wounds are comprehensively reviewed. First, the design principles and the key features of bioelectronics that can adapt to the unique wound milieu features are introduced. Next, the current state of wound biosensors and on-demand therapeutic systems are summarized and highlighted. Furthermore, we discuss the design criteria of the integrated closed loop devices. Finally, the future perspectives and challenges in wearable bioelectronics for wound care are discussed.
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Affiliation(s)
- Canran Wang
- Andrew and Peggy Cherng Department of Medical Engineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA
| | - Ehsan Shirzaei Sani
- Andrew and Peggy Cherng Department of Medical Engineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA
| | - Wei Gao
- Andrew and Peggy Cherng Department of Medical Engineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA
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Lei L, Huang W, Liu K, Liu X, Dai M, Liu Z, Zhiao Y. Trilazad mesylate-loaded electrospun cellulose acetate nanofibrous wound dressings promote diabetic wound healing by modulation of immune response and protection against oxidative damage. J Drug Deliv Sci Technol 2022. [DOI: 10.1016/j.jddst.2021.102863] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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46
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Yang X, Zhang C, Deng D, Gu Y, Wang H, Zhong Q. Multiple Stimuli-Responsive MXene-Based Hydrogel as Intelligent Drug Delivery Carriers for Deep Chronic Wound Healing. SMALL (WEINHEIM AN DER BERGSTRASSE, GERMANY) 2022; 18:e2104368. [PMID: 34821453 DOI: 10.1002/smll.202104368] [Citation(s) in RCA: 123] [Impact Index Per Article: 41.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/23/2021] [Revised: 09/24/2021] [Indexed: 06/13/2023]
Abstract
Chronic wound healing is an important and basic issue in medical and healthcare fields. Recently, stimuli-responsive hydrogel systems have emerged as promising drug delivery carriers for wound management. However, given to the limited therapeutic outcomes, new hydrogel systems for efficient wound treatment are urgently needed. Here, the development of a 2D MXene-based hydrogel system for highly efficient photo- and magnetic-responsive drug delivery oriented to deep chronic wounds repair is presented. The intelligent responsive MXene-based hydrogel drug delivery system is composed of MXene-wrapped magnetic colloids and poly(N-isopropyl acrylamide)-alginate dual-network hydrogels. It is demonstrated that the MXene-based hydrogel system exhibits multiple response capability and controllable drug delivery ability, which can reduce the toxic side effects of drugs and promote the wound healing process as well. Notably, the practical performance of the MXene-based hydrogel drug delivery system is demonstrated by applying it to the treatment of the full-thickness cutaneous wound and subcutaneous infected wound of the rat model, which indicates the great prospect in clinical wound healing and other related biomedical fields.
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Affiliation(s)
- Xin Yang
- Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China
| | - Changqing Zhang
- Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China
| | - Dawei Deng
- Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China
| | - Yueqing Gu
- State Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China
| | - Huan Wang
- The Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen, 518033, China
| | - Qifeng Zhong
- Department of Pharmaceutical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 211198, China
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47
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Sun X, Zhang Y, Ma C, Yuan Q, Wang X, Wan H, Wang P. A Review of Recent Advances in Flexible Wearable Sensors for Wound Detection Based on Optical and Electrical Sensing. BIOSENSORS 2021; 12:10. [PMID: 35049637 PMCID: PMC8773881 DOI: 10.3390/bios12010010] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/24/2021] [Revised: 12/16/2021] [Accepted: 12/17/2021] [Indexed: 05/27/2023]
Abstract
Chronic wounds that are difficult to heal can cause persistent physical pain and significant medical costs for millions of patients each year. However, traditional wound care methods based on passive bandages cannot accurately assess the wound and may cause secondary damage during frequent replacement. With advances in materials science and smart sensing technology, flexible wearable sensors for wound condition assessment have been developed that can accurately detect physiological markers in wounds and provide the necessary information for treatment decisions. The sensors can implement the sensing of biochemical markers and physical parameters that can reflect the infection and healing process of the wound, as well as transmit vital physiological information to the mobile device through optical or electrical signals. Most reviews focused on the applicability of flexible composites in the wound environment or drug delivery devices. This paper summarizes typical biochemical markers and physical parameters in wounds and their physiological significance, reviews recent advances in flexible wearable sensors for wound detection based on optical and electrical sensing principles in the last 5 years, and discusses the challenges faced and future development. This paper provides a comprehensive overview for researchers in the development of flexible wearable sensors for wound detection.
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Affiliation(s)
- Xianyou Sun
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
| | - Yanchi Zhang
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
| | - Chiyu Ma
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
| | - Qunchen Yuan
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
| | - Xinyi Wang
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
| | - Hao Wan
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
- Binjiang Institute of Zhejiang University, Hangzhou 310053, China
| | - Ping Wang
- Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; (X.S.); (Y.Z.); (C.M.); (Q.Y.); (X.W.)
- Binjiang Institute of Zhejiang University, Hangzhou 310053, China
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48
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Iversen M, Monisha M, Agarwala S. Flexible, Wearable and Fully-printed Smart Patch for pH and Hydration Sensing in Wounds. Int J Bioprint 2021; 8:447. [PMID: 35187277 PMCID: PMC8852199 DOI: 10.18063/ijb.v8i1.447] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/30/2021] [Accepted: 11/15/2021] [Indexed: 12/11/2022] Open
Abstract
Wound healing is a complex and dynamic regeneration process, wherein the physical and chemical parameters are continuously changing. Its management and monitoring can provide immense benefits, especially for bed-ridden patients. This work reports a low-cost, flexible, and fully printed on-skin patch sensor to measure the change in pH and fluid content in a wound. Such a bendable sensor can also be easily incorporated in a wound dressing. The sensor consists of different electrodes printed on polydimethylsiloxane (PDMS) substrate for pH and moisture sensing. The fabricated sensor patch has a sensitivity of 7.1 ohm/pH for wound pH levels. The hydration sensor results showed that moisture levels on a semi-porous surface can be quantified through resistance change.
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Affiliation(s)
- Mick Iversen
- Department of Electrical and Computer Engineering, Finlandsgade 22, Aarhus University, Aarhus, Denmark
| | - Monisha Monisha
- Department of Electrical and Computer Engineering, Finlandsgade 22, Aarhus University, Aarhus, Denmark
| | - Shweta Agarwala
- Department of Electrical and Computer Engineering, Finlandsgade 22, Aarhus University, Aarhus, Denmark
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49
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Zhang Y, Luo J, Zhang Q, Deng T. Growth factors, as biological macromolecules in bioactivity enhancing of electrospun wound dressings for diabetic wound healing: A review. Int J Biol Macromol 2021; 193:205-218. [PMID: 34627847 DOI: 10.1016/j.ijbiomac.2021.09.210] [Citation(s) in RCA: 37] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/25/2021] [Revised: 09/30/2021] [Accepted: 09/30/2021] [Indexed: 01/07/2023]
Abstract
Impaired wound healing is of the most conspicuous characteristics of diabetic mellitus. Reduced blood flow, chronic inflammatory reactions, infection, endothelial dysfunction, elevated levels of reactive oxygen species, and metabolic disorders cause wounds to heal more slowly in these patients. Previous studies have reported useful impacts of growth factors in management of such wounds. However, due to their short half-life and low stability, a suitable delivery platform with sustained release profile may boost their healing potential. Controlled and localized delivery of growth factors via electrospun fibers have been extensively explored in previous studies. The electrospinning method; although not new, has turned out to be extremely effective for the preparation of delivery carriers for growth factors. Due to their structural resemblance to native tissues' extracellular matrix, high encapsulation efficacy, tunability, and high surface to volume ratio, electrospun scaffolds have gained significant attention in drug delivery and tissue engineering. In the current review, careful integration of current research regarding the applications of growth factors' delivery through electrospun fibers in diabetic wounds healing has been done. This review will not only give an insight into the current updates, but will also highlights the future perspectives and challenges.
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Affiliation(s)
- Yunwu Zhang
- Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China
| | - Jingsong Luo
- Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
| | - Qi Zhang
- School of Nursing, Peking University, Beijing 100191, China
| | - Tingting Deng
- Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
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50
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Yusufu D, Magee E, Gilmore B, Mills A. Non-invasive, 3D printed, colourimetric, early wound-infection indicator. Chem Commun (Camb) 2021; 58:439-442. [PMID: 34901973 DOI: 10.1039/d1cc06147j] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
A simple, non-invasive, colour-based carbon dioxide (CO2) indicator is described. The indicator provides an indirect response to the rapid, aerobic microbial colonisation of an underlying wound when used in conjunction with an occlusive (i.e. sealed) dressing. The indicator has potential as an early warning indicator of infection in chronic wounds.
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Affiliation(s)
- Dilidaer Yusufu
- School of Chemistry and Chemical Engineering, Queens University Belfast, David Keir Building, Stranmillis Road, Belfast, BT9 5AG, UK.
| | - Erin Magee
- School of Pharmacy, Queen's University Belfast, Belfast, BT9 7BL, UK
| | - Brendan Gilmore
- School of Pharmacy, Queen's University Belfast, Belfast, BT9 7BL, UK
| | - Andrew Mills
- School of Chemistry and Chemical Engineering, Queens University Belfast, David Keir Building, Stranmillis Road, Belfast, BT9 5AG, UK.
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