|
1
|
Tian Y, Chen L, Liu X, Chang Y, Xia R, Zhang J, Kong Y, Gong Y, Li T, Wang G, Zhang Q. Colored Cellulose Nanoparticles with High Stability and Easily Modified Surface for Accurate and Sensitive Multiplex Lateral Flow Assay. ACS NANO 2025; 19:4704-4717. [PMID: 39844662 DOI: 10.1021/acsnano.4c15340] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/24/2025]
Abstract
Decentralized testing using multiplex lateral flow assays (mLFAs) to simultaneously detect multiple analytes can significantly enhance detection efficiency, reduce cost and time, and improve analytic accuracy. However, the challenges, including the monochromatic color of probe particles, interference between different test lines, and reduced specificity and sensitivity, severely hinder mLFAs from wide use. In this study, we prepared polydopamine (PDA)-coated dyed cellulose nanoparticles (dCNPs@P) with tunable colors as the probe for mLFAs. Cellulose nanoparticles (CNPs) were synthesized with uniform spheric shapes and tunable sizes. Dye molecules were loaded on CNPs via a mature industrial dyeing method. The PDA shell provided a reactive surface for facile receptor conjugation and protected the dye from leaking. dCNPs@P displayed a higher signal intensity than gold nanoparticles. They also had higher stability to tolerate salt and varied pH. The dCNP@P-based mLFAs were successfully applied to detect multiple mycotoxins in cereals and determine the levels of inflammatory biomarkers to differentiate between viral and bacterial infections. The tests represented high specificity and accuracy and were more sensitive than the tests using gold nanoparticles. The quantified detection was accessible by measuring the intensities of the colorimetric or photothermal signals. Overall, this study provides a practical system solution for mLFAs based on colored dCNPs@P.
Collapse
Affiliation(s)
- Yichen Tian
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| | - Lei Chen
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| | - Xiaodi Liu
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| | - Yuqing Chang
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| | - Ruicai Xia
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| | - Jing Zhang
- Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China
| | - Yuele Kong
- Department of Laboratory Medicine, Changzheng Hospital, Naval Medical University, Shanghai 200003, P. R. China
| | - Yao Gong
- Department of Stomatology, Changzheng Hospital, Naval Medical University, Shanghai 200003, P. R. China
| | - Tao Li
- Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China
| | - Guodong Wang
- Department of Stomatology, Changzheng Hospital, Naval Medical University, Shanghai 200003, P. R. China
| | - Qiang Zhang
- Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China
| |
Collapse
|
|
2
|
Rabbani G, Khan ME, Ahmad E, Khan MV, Ahmad A, Khan AU, Ali W, Zamzami MA, Bashiri AH, Zakri W. Serum CRP biomarker detection by using carbon nanotube field-effect transistor (CNT-FET) immunosensor. Bioelectrochemistry 2023; 153:108493. [PMID: 37392576 DOI: 10.1016/j.bioelechem.2023.108493] [Citation(s) in RCA: 10] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/19/2023] [Revised: 06/18/2023] [Accepted: 06/19/2023] [Indexed: 07/03/2023]
Abstract
C-reactive protein (CRP) is produced by the liver in response to systemic inflammation caused by bacterial infection, trauma and internal organ failures. CRP serves as a potential biomarker in the precise diagnosis of cardiovascular risk, type-2 diabetes, metabolic syndrome, hypertension and various types of cancers. The pathogenic conditions indicated above are diagnosed by an elevated CRP level in the serum. In this study, we successfully fabricated a highly sensitive and selective carbon nanotube field-effect transistor (CNT-FET) immunosensor for the detection of CRP. The CNTs were deposited on the Si/SiO2 surface, between source-drain electrodes, afterwards modified with well-known linker PBASE and then anti-CRP was immobilized. This anti-CRP functionalized CNT-FET immunosensor exhibits a wide dynamic detection range (0.01-1000 μg/mL) CRP detection, rapid response time (2-3 min) and low variation (<3 %) which can be delivered as a low-cost and rapid clinical detection technology for the early diagnosis of coronary heart disease (CHD). For the clinical applications, our sensor was tested using CRP fortified serum samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). This CNT-FET immunosensor will be helpful in taking over the complex laboratory-based expensive traditional CRP diagnostic procedures practiced in the hospitals.
Collapse
Affiliation(s)
- Gulam Rabbani
- IT-medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea
| | - Mohammad Ehtisham Khan
- Department of Chemical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia.
| | - Ejaz Ahmad
- Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
| | - Mohsin Vahid Khan
- Department of Biosciences, Integral University, Lucknow 226026, India
| | - Abrar Ahmad
- Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia
| | - Anwar Ulla Khan
- Department of Electrical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia
| | - Wahid Ali
- Department of Chemical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia
| | - Mazin A Zamzami
- Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia
| | - Abdullateef H Bashiri
- Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia
| | - Waleed Zakri
- Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia
| |
Collapse
|
|
3
|
Yuan Z, Han M, Li D, Hao R, Guo X, Sang S, Zhang H, Ma X, Jin H, Xing Z, Zhao C. A cost-effective smartphone-based device for rapid C-reaction protein (CRP) detection using magnetoelastic immunosensor. LAB ON A CHIP 2023; 23:2048-2056. [PMID: 36916284 DOI: 10.1039/d2lc01065h] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/05/2023]
Abstract
C-Reaction protein (CRP) is a marker of nonspecific immunity for vital signs and wound assessment, and it can be used to diagnose infections in clinical medicine. However, measuring CRP level currently requires hospital-based instruments, high-cost reagents, and a complex process, all of which have limited its full capabilities for self-detection, a growing trend in modern medicine. In this study, we developed a novel smartphone-based device using advanced methods of magnetoelastic immunosensing to mitigate these limitations. We combined a system-on-chip (SoC) hardware architecture with smartphone apps to realize the sampling of resonance frequency shift on magnetoelastic chips, which can determine the ultra-sensitivity to mass change caused by the binding of anti-CRP antibody and CRP. Through detecting a multi-group of samples, we found that the resonance frequency shift was linearly proportional to the CRP concentration in the range from 0.1 to 100 μg mL-1, with a sensitivity of 12.90 Hz μg-1 mL-1 and a detection limit of 2.349 × 10-4 μg mL-1. Meanwhile, compared with the large-scale instrument used in clinical settings, the performance of our device was stable and significantly more portable, rapid and cost-effective, offering excellent potential for modern home-based diagnosis.
Collapse
Affiliation(s)
- Zhongyun Yuan
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Mengshu Han
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Donghao Li
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Runfang Hao
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Xing Guo
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Shengbo Sang
- Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
- Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China
| | - Hongpeng Zhang
- Department of Vascular Surgery, Chinese PLA General Hospital, 100853, Beijing, China
| | - Xingyi Ma
- School of Science, Harbin Institute of Technology, Shenzhen, Guangdong 518055, China
| | - Hu Jin
- Division of Electrical Engineering, Hanyang University, 15588 Ansan, Republic of Korea
| | - Zhijin Xing
- Department of Ultrasound Medicine, Shenzhen Hospital of the University of Hong Kong, 518053, Shenzhen, China
| | - Chun Zhao
- College of Information and Communication Engineering, Sungkyunkwan University, Chunchun-Dong, Changan-Ku, 440746 Suwon, Republic of Korea.
| |
Collapse
|
|
4
|
Jin B, Du Z, Zhang C, Yu Z, Wang X, Hu J, Li Z. Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection. BIOSENSORS 2022; 12:977. [PMID: 36354486 PMCID: PMC9688000 DOI: 10.3390/bios12110977] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 09/21/2022] [Revised: 10/25/2022] [Accepted: 11/01/2022] [Indexed: 06/16/2023]
Abstract
Inflammation caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may portend chronic diseases; therefore, high-sensitivity CRP (hs-CRP) detection in a quantitative, rapid, user-friendly, and low-cost manner is highly demanded. In this paper, we developed a europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) integrating with a benchtop fluorescence analyzer for hs-CRP detection. The optimization of the EuPSM-based LFIA was implemented through adjusting the antibody density on EuPSM from 100% to 60% of the saturated density. Finally, the limit of detection of 0.76 pg/mL and detection range of 0.025-250 ng/mL were obtained. Moreover, the clinical application capability of the proposed platform was validated through detecting CRP in clinical serum samples, showing high consistency with the results obtained from the clinical standard method. Hence, the proposed EuPSM-based LFIA has been verified to be well suitable for hs-CRP detection, while also showing great applicability for sensitively and rapidly detecting other biomarkers.
Collapse
Affiliation(s)
- Birui Jin
- School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, China
- Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an 710049, China
| | - Zhiguo Du
- School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, China
| | - Chuyao Zhang
- School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an 710021, China
| | - Zhao Yu
- Xi’an Thermal Power Research Institute Co., Ltd., Xi’an 710054, China
| | - Xuemin Wang
- The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
- Department of Radiotherapy Hospital Unit Radiation Therapy, Shaanxi Provincial Tumor Hospital, Xi’an 710061, China
| | - Jie Hu
- Suzhou DiYinAn Biotech Co., Ltd., Suzhou Innovation Center for Life Science and Technology, Suzhou 215129, China
| | - Zedong Li
- Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an 710049, China
- The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
| |
Collapse
|
|
5
|
Multifunctional self-driven origami paper-based integrated microfluidic chip to detect CRP and PAB in whole blood. Biosens Bioelectron 2022; 208:114225. [DOI: 10.1016/j.bios.2022.114225] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/17/2021] [Revised: 03/01/2022] [Accepted: 03/24/2022] [Indexed: 12/12/2022]
|
|
6
|
Weng G, Shen X, Li J, Wang J, Zhu J, Zhao J. A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids. Anal Chim Acta 2022; 1221:340129. [DOI: 10.1016/j.aca.2022.340129] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/25/2022] [Revised: 06/10/2022] [Accepted: 06/24/2022] [Indexed: 11/01/2022]
|
|
7
|
Pohanka M. Diagnoses Based on C-Reactive Protein Point-of-Care Tests. BIOSENSORS 2022; 12:bios12050344. [PMID: 35624645 PMCID: PMC9138282 DOI: 10.3390/bios12050344] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/18/2022] [Revised: 05/09/2022] [Accepted: 05/16/2022] [Indexed: 05/09/2023]
Abstract
C-reactive protein (CRP) is an important part of the immune system's reaction to various pathological impulses such as bacterial infections, systemic inflammation, and internal organ failures. An increased CRP level serves to diagnose the mentioned pathological states. Both standard laboratory methods and simple point-of-care devices such as lateral flow tests and immunoturbidimetric assays serve for the instrumental diagnoses based on CRP. The current method for CRP has many flaws and limitations in its use. Biosensor and bioassay analytical devices are presently researched by many teams to provide more sensitive and better-suited tools for point-of-care tests of CRP in biological samples when compared to the standard methods. This review article is focused on mapping the diagnostical relevance of CRP, the applicability of the current analytical methods, and the recent innovations in the measurement of CRP level.
Collapse
Affiliation(s)
- Miroslav Pohanka
- Faculty of Military Health Sciences, University of Defense, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic
| |
Collapse
|
|
8
|
Firoozbakhtian A, Rezayan AH, Hajghassem H, Rahimi F, Ghazani MF, Kalantar M, Mohamadsharifi A. Buried-Gate MWCNT FET-Based Nanobiosensing Device for Real-Time Detection of CRP. ACS OMEGA 2022; 7:7341-7349. [PMID: 35252724 PMCID: PMC8892644 DOI: 10.1021/acsomega.1c07271] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/24/2021] [Accepted: 02/01/2022] [Indexed: 06/14/2023]
Abstract
C-reactive protein (CRP), an acute-phase protein synthesized in the liver in response to inflammation, is one of the biomarkers used for the detection of several diseases. Sepsis and cardiovascular diseases are two of the most important diseases for which detection of CRP at very early stages in the clinical range can help avert serious consequences. Here, a CNT-based nanobiosensing system, which is portable and reproducible, is used for label-free, online detection of CRP. The system consists of an aptameric CNT-based field-effect transistor benefiting from a buried gate geometry with Al2O3 as a high dielectric layer and can reflect the pro-cytokine concentration. Test results show that the device responds to CRP changes within 8 min, with a limit of detection as low as 150 pM (0.017 mg L-1). The device was found to have a linear behavior in the range of 0.43-42.86 nM (0.05-5 mg L-1). The selectivity of the device was tested with TNF-α, IL-6, and BSA, to which the nanosensing system showed no significant response compared with CRP. The device showed good stability for 14 days and was completely reproducible during this period. These findings indicate that the proposed portable system is a potential candidate for CRP measurements in the clinical range.
Collapse
Affiliation(s)
- Ali Firoozbakhtian
- Division
of Nanobiotechnology, Department of Life Sciences Engineering, Faculty
of New Sciences and Technologies, University
of Tehran, P.O. Box 14395-1561 Tehran 1439957131, Iran
| | - Ali Hossein Rezayan
- Division
of Nanobiotechnology, Department of Life Sciences Engineering, Faculty
of New Sciences and Technologies, University
of Tehran, P.O. Box 14395-1561 Tehran 1439957131, Iran
| | - Hassan Hajghassem
- MEMS
& NEMS Laboratory, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
| | - Fereshteh Rahimi
- Division
of Nanobiotechnology, Department of Life Sciences Engineering, Faculty
of New Sciences and Technologies, University
of Tehran, P.O. Box 14395-1561 Tehran 1439957131, Iran
| | - Masoud Faraghi Ghazani
- MEMS
& NEMS Laboratory, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
| | - Mahsa Kalantar
- Division
of Nanobiotechnology, Department of Life Sciences Engineering, Faculty
of New Sciences and Technologies, University
of Tehran, P.O. Box 14395-1561 Tehran 1439957131, Iran
| | - Amir Mohamadsharifi
- MEMS
& NEMS Laboratory, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
| |
Collapse
|
|
9
|
Sharma A, Tok AIY, Alagappan P, Liedberg B. Point of care testing of sports biomarkers: Potential applications, recent advances and future outlook. Trends Analyt Chem 2021. [DOI: 10.1016/j.trac.2021.116327] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
|
|
10
|
Development of a Point-of-Care System Based on White Light Reflectance Spectroscopy: Application in CRP Determination. BIOSENSORS-BASEL 2021; 11:bios11080268. [PMID: 34436070 PMCID: PMC8394791 DOI: 10.3390/bios11080268] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/03/2021] [Revised: 07/26/2021] [Accepted: 08/05/2021] [Indexed: 02/07/2023]
Abstract
The development of methods and miniaturized systems for fast and reliable quantitative determinations at the Point-of-Care is a top challenge and priority in diagnostics. In this work, a compact bench-top system, based on White Light Reflectance Spectroscopy, is introduced and evaluated in an application with high clinical interest, namely the determination of C-Reactive protein (CRP) in human blood samples. The system encompassed all the necessary electronic and optical components for the performance of the assay, while the dedicated software provided the sequence and duration of assay steps, the reagents flow rate, the real-time monitoring of sensor response, and data processing to deliver in short time and accurately the CPR concentration in the sample. The CRP assay included two steps, the first comprising the binding of sample CRP onto the chip immobilized capture antibody and the second the reaction of the surface immunosorbed CRP molecules with the detection antibody. The assay duration was 12 min and the dynamic range was from 0.05 to 200 μg/mL, covering both normal values and acute inflammation incidents. There was an excellent agreement between CRP values determined in human plasma samples using the developed device with those received for the same samples by a standard diagnostic laboratory method.
Collapse
|
|
11
|
Li S, Huang S, Ke Y, Chen H, Dang J, Huang C, Liu W, Cui D, Wang J, Zhi X, Ding X. A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2. ADVANCED FUNCTIONAL MATERIALS 2021; 31:2100801. [PMID: 34230825 PMCID: PMC8250055 DOI: 10.1002/adfm.202100801] [Citation(s) in RCA: 13] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/25/2021] [Revised: 03/19/2021] [Indexed: 05/03/2023]
Abstract
Nowadays, the main obstacle for further miniaturization and integration of nucleic acids point-of-care testing devices is the lack of low-cost and high-performance heating materials for supporting reliable nucleic acids amplification. Herein, reduced graphene oxide hybridized multi-walled carbon nanotubes nano-circuit integrated into an ingenious paper-based heater is developed, which is integrated into a paper-based analytical device (named HiPAD). The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still raging across the world. As a proof of concept, the HiPAD is utilized to visually detect the SARS-CoV-2 N gene using colored loop-mediated isothermal amplification reaction. This HiPAD costing a few dollars has comparable detection performance to traditional nucleic acids amplifier costing thousands of dollars. The detection range is from 25 to 2.5 × 1010 copies mL-1 in 45 min. The detection limit of 25 copies mL-1 is 40 times more sensitive than 1000 copies mL-1 in conventional real-time PCR instruments. The disposable paper-based chip could also avoid potential secondary transmission of COVID-19 by convenient incineration to guarantee biosafety. The HiPAD or easily expanded M-HiPAD (for multiplex detection) has great potential for pathogen diagnostics in resource-limited settings.
Collapse
Affiliation(s)
- Sijie Li
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Shiyi Huang
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Yuqing Ke
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Hongjun Chen
- Shanghai Veterinary Research Institute518 Ziyue Road, Minhang DistrictShanghai200241China
| | - Jingqi Dang
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Chengjie Huang
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Wenjia Liu
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Daxiang Cui
- Shanghai Engineering Center for Intelligent Diagnosis and Treatment InstrumentSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong University800 Dongchuan RD, Minghang DistrictShanghai200240China
| | - Jinglin Wang
- State Key Laboratory of Pathogen and BiosecurityInstitute of Microbiology and Epidemiology20 Dongda Street, Fengtai DistrictBeijing100071China
| | - Xiao Zhi
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| | - Xianting Ding
- State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University1954 Huashan RD, Xuhui DistrictShanghai200030China
| |
Collapse
|
|
12
|
Li Y, Liu X, Guo J, Zhang Y, Guo J, Wu X, Wang B, Ma X. Simultaneous Detection of Inflammatory Biomarkers by SERS Nanotag-Based Lateral Flow Assay with Portable Cloud Raman Spectrometer. NANOMATERIALS (BASEL, SWITZERLAND) 2021; 11:1496. [PMID: 34198765 PMCID: PMC8226521 DOI: 10.3390/nano11061496] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/16/2021] [Revised: 05/29/2021] [Accepted: 05/31/2021] [Indexed: 01/22/2023]
Abstract
Inflammatory biomarkers are closely related to infectious diseases. However, traditional clinical tests of laboratory inspection are unable to achieve rapid and accurate detection of these biomarkers on-site due to shortcomings such as complex experimental operation, expensive equipment, and long test time. Herein, we proposed a lateral flow assay (LFA) strip based on surface-enhanced Raman scattering (SERS) nanotags (SERS-LFA strips) for the simultaneous and quantitative detection of dual infection biomarkers, serum amyloid A (SAA) and C-reactive protein (CRP), respectively. In practice, mesoporous silica (mSiO2)-coated Au nanoparticles (Au NPs) were used as the SERS substrate. Mercaptobenzoic acid (MBA) was embedded in the internal gap between Au NPs and the mSiO2 shell to prepare AuMBA@mSiO2 NPs, onto which SAA and CRP antibodies were modified to prepare two AuMBA@mSiO2 SERS nanotags. The Raman intensities of the test and control lines were simultaneously identified for the qualitative detection of SAA and CRP, with limits of detection (LODs) as low as 0.1 and 0.05 ng/mL for SAA and CRP, respectively. Finally, aiming at point-of-care testing (POCT) applications, we used a smartphone-based portable Raman spectrometer to quantitatively analyze the SERS-LFA strips. The Raman signal could still be accurately detected when the concentration of SAA and CRP was 10 ng/mL, which is lower than the LOD required in clinical practice for most diseases. Therefore, taking into account its simple operation and short analysis time, by using a portable Raman spectrometer which can be equipped with a 5G cloud-based healthcare management system, the current strategy based on SERS-LFA provides the potential for the quick and on-site diagnosis of infectious diseases such as sepsis, which is of great significance for medical guidance on the treatment of widely spread infection-related diseases in remote areas that lack well-developed medical resources.
Collapse
Affiliation(s)
- Yang Li
- School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China; (Y.L.); (Y.Z.)
| | - Xiaojia Liu
- Sauvage Laboratory for Smart Materials, School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China;
- Shenzhen Bay Laboratory, No. 9 Duxue Road, Shenzhen 518055, China
| | - Jiuchuan Guo
- School of Communication and Information Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China; (J.G.); (J.G.)
| | - Yueting Zhang
- School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China; (Y.L.); (Y.Z.)
| | - Jinhong Guo
- School of Communication and Information Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China; (J.G.); (J.G.)
| | - Xinggui Wu
- CloudMinds Inc., Shenzhen Bay Science and Technology Ecological Park, Shenzhen 100022, China
| | - Bo Wang
- School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China; (Y.L.); (Y.Z.)
| | - Xing Ma
- Sauvage Laboratory for Smart Materials, School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China;
- Shenzhen Bay Laboratory, No. 9 Duxue Road, Shenzhen 518055, China
| |
Collapse
|
|
13
|
Meng F, Zhang L, Huo W, Lian J, Jesorka A, Shi X, Gao Y. Dynamic Range Expansion of the C-Reactive Protein Quantification with a Tandem Giant Magnetoresistance Biosensor. ACS OMEGA 2021; 6:12923-12930. [PMID: 34056444 PMCID: PMC8154231 DOI: 10.1021/acsomega.1c01603] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/25/2021] [Accepted: 04/26/2021] [Indexed: 05/03/2023]
Abstract
In this study, we report a convenient analytical method for a full-range quantification of the C-reactive protein (CRP), a blood biomarker of infection and cardiovascular events. We determine CRP over the entire diagnostically relevant concentration range in undiluted human blood serum in a single test, using a tandem giant magnetoresistance (GMR) sensor. The tandem principle combines a sandwich assay and a competitive assay, which allows for the discrimination of the concentration values resulting from the multivalued dose-response curve ("Hook" effect), which characterizes the one-step sandwich assay at high CRP concentrations. The sensor covers a linear detection range for CRP concentration from 3 ng/mL to 350 μg/mL, the detection limit (s/n = 3) is 1 ng/mL. The prominent features of the chip-based method are its expanded dynamic range and low sample volume (50 μL), and the need for a short measurement time of 15 min. These figures of merit, in addition to the low detection limit equal to the established assay instrumentation, make it a viable candidate for use in point-of-care diagnostics.
Collapse
Affiliation(s)
- Fanda Meng
- Department
of Clinical Laboratory Medicine, The First Affiliated Hospital of
Shandong First Medical University & Shandong Provincial Qianfoshan
Hospital, Shandong Medicine and Health Key Laboratory of Laboratory
Medicine, Jinan 250014, China
- School
of Basic Medicine, Shandong First Medical
University & Shandong Academy of Medical Sciences, Jinan 250062, China
- Department
of Chemistry and Chemical Engineering, Chalmers
University of Technology, Gothenburg SE-412 96, Sweden
- ,
| | - Lei Zhang
- Key
Laboratory of Photochemical Conversion and Optoelectronic Materials,
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
- University
of Chinese Academy of Sciences, Beijing 100149, China
- Dongguan
Bosh Biotechnologies, Ltd., Guangdong 523808, China
| | - Weisong Huo
- Dongguan
Bosh Biotechnologies, Ltd., Guangdong 523808, China
| | - Jie Lian
- College
of Criminal Investigation, People’s
Public Security University of China, Beijing 100038, China
| | - Aldo Jesorka
- Department
of Chemistry and Chemical Engineering, Chalmers
University of Technology, Gothenburg SE-412 96, Sweden
| | - Xizeng Shi
- Dongguan
Bosh Biotechnologies, Ltd., Guangdong 523808, China
| | - Yunhua Gao
- Key
Laboratory of Photochemical Conversion and Optoelectronic Materials,
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
- University
of Chinese Academy of Sciences, Beijing 100149, China
| |
Collapse
|
|
14
|
|
|
15
|
Sadighbayan D, Hasanzadeh M, Ghafar-Zadeh E. Biosensing based on field-effect transistors (FET): Recent progress and challenges. Trends Analyt Chem 2020; 133:116067. [PMID: 33052154 PMCID: PMC7545218 DOI: 10.1016/j.trac.2020.116067] [Citation(s) in RCA: 108] [Impact Index Per Article: 21.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
Abstract
The use of field-Effect-Transistor (FET) type biosensing arrangements has been highlighted by researchers in the field of early biomarker detection and drug screening. Their non-metalized gate dielectrics that are exposed to an electrolyte solution cover the semiconductor material and actively transduce the biological changes on the surface. The efficiency of these novel devices in detecting different biomolecular analytes in a real-time, highly precise, specific, and label-free manner has been validated by numerous research studies. Considerable progress has been attained in designing FET devices, especially for biomedical diagnosis and cell-based assays in the past few decades. The exceptional electronic properties, compactness, and scalability of these novel tools are very desirable for designing rapid, label-free, and mass detection of biomolecules. With the incorporation of nanotechnology, the performance of biosensors based on FET boosts significantly, particularly, employment of nanomaterials such as graphene, metal nanoparticles, single and multi-walled carbon nanotubes, nanorods, and nanowires. Besides, their commercial availability, and high-quality production on a large-scale, turn them to be one of the most preferred sensing and screening platforms. This review presents the basic structural setup and working principle of different types of FET devices. We also focused on the latest progression regarding the use of FET biosensors for the recognition of viruses such as, recently emerged COVID-19, Influenza, Hepatitis B Virus, protein biomarkers, nucleic acids, bacteria, cells, and various ions. Additionally, an outline of the development of FET sensors for investigations related to drug development and the cellular investigation is also presented. Some technical strategies for enhancing the sensitivity and selectivity of detection in these devices are addressed as well. However, there are still certain challenges which are remained unaddressed concerning the performance and clinical use of transistor-based point-of-care (POC) instruments; accordingly, expectations about their future improvement for biosensing and cellular studies are argued at the end of this review.
Collapse
Affiliation(s)
- Deniz Sadighbayan
- Biologically Inspired Sensors and Actuators (BioSA), Faculty of Science, Dept. of Biology, York University, Toronto, Canada
- Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Mohammad Hasanzadeh
- Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Ebrahim Ghafar-Zadeh
- Biologically Inspired Sensors and Actuators (BioSA), Faculty of Science, Dept. of Biology, York University, Toronto, Canada
- Dept. of Elecrical Engineering and Computer Science, Lassonde School of Engineering, York University, Toronto, Canada
| |
Collapse
|
|
16
|
Balayan S, Chauhan N, Chandra R, Kuchhal NK, Jain U. Recent advances in developing biosensing based platforms for neonatal sepsis. Biosens Bioelectron 2020; 169:112552. [PMID: 32931992 DOI: 10.1016/j.bios.2020.112552] [Citation(s) in RCA: 34] [Impact Index Per Article: 6.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/28/2020] [Revised: 08/16/2020] [Accepted: 08/23/2020] [Indexed: 12/12/2022]
Abstract
Neonatal sepsis is a bloodstream infection primarily caused by Escherichia coli (E. coli), Group B Streptococcus (GBS), Listeria monocytogenes, Haemophilus influenzae, S. aureus, Klebsiella spp. and non-typhoidal Salmonella bacteria. Neonatal Sepsis is referred as a critical response to the infection in the neonatal period that can lead to the failure of body organs and thereby causing damage to the tissues resulting in death of the neonates. Nearly 4 million deaths across the world are occurred due to neonatal sepsis infections. In order to prevent the bloodstream infections in the neonates, it is indispensable to diagnose the disease properly for appropriate treatment during the point of care. Numerous studies have been reported to identify major biomarkers associated with neonatal sepsis including Serum Amyloid A (SAA), C - reactive protein (CRP), Procalcitonin (PCT) and Lipopolysaccharide-binding protein (LBP). Distinct diagnostic platforms have also been developed detecting the presence of bloodstream infections including electrochemical, potentiometric, and impedimetric sensors. Recently, electrochemical biosensors with the integration of nanomaterials have emerged as a better platform for neonatal sepsis biomarkers detection. This review article summarizes the diverse screening platforms, evaluation parameters, and new advances based on implications of nanomaterials for the development of biosensors detecting neonatal sepsis infections. The review further elucidates the significance and future scope of distinctive platforms which are predominantly associated with detection of neonatal sepsis.
Collapse
Affiliation(s)
- Sapna Balayan
- Amity Institute of Nanotechnology, Amity University, Noida, 201313, Uttar Pradesh, India
| | - Nidhi Chauhan
- Amity Institute of Nanotechnology, Amity University, Noida, 201313, Uttar Pradesh, India
| | - Ramesh Chandra
- Department of Chemistry, University of Delhi, Delhi, 110007, India
| | | | - Utkarsh Jain
- Amity Institute of Nanotechnology, Amity University, Noida, 201313, Uttar Pradesh, India.
| |
Collapse
|
|
17
|
Review on electrochemical sensing strategies for C-reactive protein and cardiac troponin I detection. Microchem J 2020. [DOI: 10.1016/j.microc.2020.104857] [Citation(s) in RCA: 24] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/16/2022]
|
|
18
|
Gan X, Gong T, Zheng Y, Gopinath SCB, Zhao K. Electroimmunodetection of cardiac C-reactive protein for determining myocardial Injury. Biotechnol Appl Biochem 2020; 68:272-278. [PMID: 32275089 DOI: 10.1002/bab.1921] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2020] [Accepted: 04/03/2020] [Indexed: 11/07/2022]
Abstract
C-reactive protein (CRP) is an acute phase reactant to be a marker of inflammation and has been correlated with the cardiac injury. An immunoassay was performed using anti-human CRP antibody on an InterDigitated electrode (IDE) sensor to determine and specify CRP concentration for diagnosing the condition of myocardial inflammation. To promote the detection, gold nanoparticle (GNP) was seeded on the aminated-IDE surface. Anti-CRP was hitched on the GNP-seeded surface and identified the abundance of CRP. The limit of quantification was found as 100 fM, and the higher current response was noticed by increasing CRP concentrations with the sensitivity at 1 pM. Furthermore, CRP-spiked human serum did not interfere the determination of CRP and increased the current response, indicating suitability for a real-life sample. Similarly, the control experiments with nonimmune antibody Troponin I are not showing the definite current responses, proving the selective identification of CRP. This method of diagnosing is needful to determine the cardiovascular injury at the right time.
Collapse
Affiliation(s)
- Xiaoya Gan
- Department of Cardiology, Shandong Provincial Taishan Hospital, Taian, Shandong Province, China
| | - Tao Gong
- Department of Critical Medicine, Pingyi County Hospital of Traditional Chinese Medicine, Pingyi County, Linyi, Shandong Province, China
| | - Yin Zheng
- Department of Cardiology, Hainan Cancer Hospital, Xiuying District, Haikou, Hainan, China.,Hainan Chengmei International Health Management Center, Xiuying District, Haikou, Hainan, China
| | - Subash C B Gopinath
- Universiti Malaysia Perlis, Institute of Nano Electronic Engineering, Kangar, Perlis, Malaysia.,School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis, Malaysia
| | - Ketong Zhao
- Department of Cardiology, Hainan Cancer Hospital, Xiuying District, Haikou, Hainan, China.,Hainan Chengmei International Health Management Center, Xiuying District, Haikou, Hainan, China
| |
Collapse
|
|
19
|
Amperometric immunosensor based on covalent organic frameworks and Pt/Ru/C nanoparticles for the quantification of C-reactive protein. Mikrochim Acta 2020; 187:320. [DOI: 10.1007/s00604-020-04286-8] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/11/2019] [Accepted: 04/19/2020] [Indexed: 10/24/2022]
|
|
20
|
Sardesai AU, Dhamu VN, Paul A, Muthukumar S, Prasad S. Design and Electrochemical Characterization of Spiral Electrochemical Notification Coupled Electrode (SENCE) Platform for Biosensing Application. MICROMACHINES 2020; 11:E333. [PMID: 32213807 PMCID: PMC7143249 DOI: 10.3390/mi11030333] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/02/2020] [Revised: 03/22/2020] [Accepted: 03/23/2020] [Indexed: 01/08/2023]
Abstract
C-reactive protein (CRP) is considered to be an important biomarker associated with many diseases. During any physiological inflammation, the level of CRP reaches its peak at 48 h, whereas its half-life is around 19 h. Hence, the detection of low-level CRP is an important task for the prognostic management of diseases like cancer, stress, metabolic disorders, cardiovascular diseases, and so on. There are various techniques available in the market to detect low-level CRP like ELISA, Western blot, etc. An electrochemical biosensor is one of the important miniaturized platforms which provides sensitivity along with ease of operation. The most important element of an electrochemical biosensor platform is the electrode which, upon functionalization with a probe, captures the selective antibody-antigen interaction and produces a digital signal in the form of potential/current. Optimization of the electrode design can increase the sensitivity of the sensor by 5-10-fold. Herein, we come up with a new sensor design called the spiral electrochemical notification coupled electrode (SENCE) where the working electrode (WE) is concentric in nature, which shows better response than the market-available standard screen-printed electrode. The sensor is thoroughly characterized using a standard Ferro/Ferri couple. The sensing performance of the fabricated platform is also characterized by the detection of standard H2O2 using a diffusion-driven technique, and a low detection limit of 15 µM was achieved. Furthermore, we utilized the platform to detect a low level (100 ng/mL) of CRP in synthetic sweat. The manuscript provides emphasis on the design of a sensor that can offer good sensitivity in electrochemical biosensing applications.
Collapse
Affiliation(s)
- Abha Umesh Sardesai
- Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, USA
| | - Vikram Narayanan Dhamu
- Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, USA
| | - Anirban Paul
- Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, USA
| | - Sriram Muthukumar
- Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, USA
- EnLiSense LLC, 1813 Audubon Pondway, Allen, TX 75013, USA
| | - Shalini Prasad
- Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, USA
- EnLiSense LLC, 1813 Audubon Pondway, Allen, TX 75013, USA
| |
Collapse
|
|
21
|
Qureshi A, Niazi JH. Biosensors for detecting viral and bacterial infections using host biomarkers: a review. Analyst 2020; 145:7825-7848. [DOI: 10.1039/d0an00896f] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
A schematic diagram showing multiple modes of biosensing platforms for the diagnosis of bacterial or viral infections.
Collapse
Affiliation(s)
- Anjum Qureshi
- Sabanci University
- SUNUM Nanotechnology Research and Application Center
- Tuzla 34956
- Turkey
| | - Javed H. Niazi
- Sabanci University
- SUNUM Nanotechnology Research and Application Center
- Tuzla 34956
- Turkey
| |
Collapse
|
|
22
|
Oh SY, Heo NS, Bajpai VK, Jang SC, Ok G, Cho Y, Huh YS. Development of a Cuvette-Based LSPR Sensor Chip Using a Plasmonically Active Transparent Strip. Front Bioeng Biotechnol 2019; 7:299. [PMID: 31737618 PMCID: PMC6839135 DOI: 10.3389/fbioe.2019.00299] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/09/2019] [Accepted: 10/15/2019] [Indexed: 01/22/2023] Open
Abstract
This research demonstrates the development of a transmission-mode localized surface plasmon resonance (LSPR) sensor chip using a cuvette cell system for the sensitive detection of a biomolecule marker such as C-reactive protein (CRP). In order to develop a highly sensitive LSPR sensor chip, plasmonically active gold nanoparticles (AuNPs) were decorated onto various transparent substrates in the form of a uniform, high-density single layer using a self-assembly process. The transparent substrate surface was modified with amine functional groups via (3-Aminopropyl)triethoxysilane (APTES) treatment, and the ligand concentration and temperature (0.5% APTES at 60°C) were then optimized to control the binding energy with AuNPs. The optimized plasmonically active strip was subsequently prepared by dipping the amine-functionalized substrate into AuNPs for 8 h. The optimized plasmonic strip functionalized with anti-CRP was transformed into a portable LSPR sensor chip by placing it inside a cuvette cell system, and its detection performance was evaluated using CRP as a model sample. The detection limit for CRP using our LSPR sensor chip was 0.01 μg/mL, and the detection dynamic range was 0.01–10 μg/mL with a %CV of <10%, thus confirming its selectivity and good reproducibility. These findings illustrate that the highly sensitive portable LSPR biosensor developed in this study is expected to be widely used in a diverse range of fields such as diagnosis, medical care, environmental monitoring, and food quality control.
Collapse
Affiliation(s)
- Seo Yeong Oh
- Department of Biological Engineering, Inha University, Incheon, South Korea
| | - Nam Su Heo
- Department of Biological Engineering, Inha University, Incheon, South Korea.,Division of Electron Microscopic Research, Korea Basic Science Institute, Daejeon, South Korea
| | - Vivek K Bajpai
- Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul, South Korea
| | - Sung-Chan Jang
- Department of Biological Engineering, Inha University, Incheon, South Korea.,Radwaste Management Center, Korea Atomic Energy Research Institute, Daejeon, South Korea
| | - Gyeongsik Ok
- Research Group of Consumer Safety, Korea Food Research Institute, Wanju-gun, South Korea
| | - Youngjin Cho
- Research Group of Consumer Safety, Korea Food Research Institute, Wanju-gun, South Korea
| | - Yun Suk Huh
- Department of Biological Engineering, Inha University, Incheon, South Korea
| |
Collapse
|
|
23
|
A folding affinity paper-based electrochemical impedance device for cardiovascular risk assessment. Biosens Bioelectron 2019; 130:389-396. [DOI: 10.1016/j.bios.2018.09.031] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/30/2018] [Revised: 09/01/2018] [Accepted: 09/08/2018] [Indexed: 12/11/2022]
|
|
24
|
Kumar S, Tripathy S, Jyoti A, Singh SG. Recent advances in biosensors for diagnosis and detection of sepsis: A comprehensive review. Biosens Bioelectron 2018; 124-125:205-215. [PMID: 30388563 DOI: 10.1016/j.bios.2018.10.034] [Citation(s) in RCA: 102] [Impact Index Per Article: 14.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2018] [Revised: 09/28/2018] [Accepted: 10/16/2018] [Indexed: 12/12/2022]
Abstract
Sepsis is one of the leading causes of mortality among critically ill patients globally. According to WHO report 2018, it is estimated to affect beyond 30 million people worldwide every year. It causes loss of human lives, which arise from infection and inflammation and long term stay in intensive care unit (ICU) in hospitals. Despite the availability of satisfactory prognostic markers contributing to the diagnosis of sepsis, millions of people die even after admission to the hospitals. Correct and early diagnosis of sepsis leads to rapid administration of appropriate antibiotics can thus potentially avert the attainment to critical stages of sepsis, thereby saving human lives. Conventional diagnostic practices are costly, time consuming and they lack adequate sensitivity and selectivity, provoking an urgent need for developing alternate sepsis diagnosis systems. Nevertheless, biosensors have the much-treasured scope for reasonable sepsis diagnosis. Advancement in nano-biotechnology has provided new paradigm for biosensor platforms with upgraded features. Here, we provide an overview of the recent advances in biosensors with a brief introduction to sepsis, followed by the conventional methods of diagnosis and bio-sensing. To conclude, a proactive role and an outlook on technologically advanced biosensor platforms are discoursed with possible biomedical applications.
Collapse
Affiliation(s)
- Sanni Kumar
- Amity Institute of Biotechnology, Amity University, Jaipur, Rajasthan 303002, India.
| | | | - Anupam Jyoti
- Amity Institute of Biotechnology, Amity University, Jaipur, Rajasthan 303002, India.
| | | |
Collapse
|
|
25
|
Sonuç Karaboğa MN, Sezgintürk MK. Determination of C-reactive protein by PAMAM decorated ITO based disposable biosensing system: A new immunosensor design from an old molecule. Talanta 2018; 186:162-168. [DOI: 10.1016/j.talanta.2018.04.051] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/01/2018] [Revised: 04/12/2018] [Accepted: 04/17/2018] [Indexed: 12/18/2022]
|
|
26
|
Juang DS, Lin CH, Huo YR, Tang CY, Cheng CR, Wu HS, Huang SF, Kalnitsky A, Lin CC. Proton-ELISA: Electrochemical immunoassay on a dual-gated ISFET array. Biosens Bioelectron 2018; 117:175-182. [PMID: 29902633 DOI: 10.1016/j.bios.2018.06.012] [Citation(s) in RCA: 26] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/26/2018] [Revised: 05/22/2018] [Accepted: 06/05/2018] [Indexed: 11/30/2022]
Abstract
Here we report an electrochemical immunoassay platform called Proton-ELISA (H-ELISA) for the detection of bioanalytes. H-ELISA uniquely utilizes protons as an immunoassay detection medium, generated by the enzyme glucose oxidase (GOx) coupled with Fenton's reagent in a proton amplification reaction cascade that results in a highly amplified signal. A proton-sensitive dual-gated ion-sensitive field effect transistor (DG-ISFET) sensor was also developed for sensitive and accurate detection of the proton signal in H-ELISA. The DG-ISFET sensor comprises of a 128 × 128 array of 16,384 sensing transistors each with an individually addressable back gate to allow for a very high signal throughput and improved accuracy. We then demonstrated that the platform could detect C-reactive protein and immunoglobulin E down to concentrations of 12.5 and 125 pg/mL, respectively. We further showed that the platform is compatible with complex biological sample conditions such as human serum, suggesting that the platform is sufficiently robust for potential diagnostic applications.
Collapse
Affiliation(s)
- Duane S Juang
- Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan
| | - Ching-Hui Lin
- Taiwan Semiconductor Manufacturing Company, 8, Li-Hsin Rd. 6, Hsinchu Science Park, Hsinchu 30077, Taiwan
| | - Yi-Ren Huo
- Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan
| | - Chia-Yu Tang
- Institute of NanoEngineering and MicroSystems, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan
| | - Chun-Ren Cheng
- Taiwan Semiconductor Manufacturing Company, 8, Li-Hsin Rd. 6, Hsinchu Science Park, Hsinchu 30077, Taiwan
| | - Hua-Shu Wu
- Taiwan Semiconductor Manufacturing Company, 8, Li-Hsin Rd. 6, Hsinchu Science Park, Hsinchu 30077, Taiwan
| | - Shih-Fen Huang
- Taiwan Semiconductor Manufacturing Company, 8, Li-Hsin Rd. 6, Hsinchu Science Park, Hsinchu 30077, Taiwan
| | - Alexander Kalnitsky
- Taiwan Semiconductor Manufacturing Company, 8, Li-Hsin Rd. 6, Hsinchu Science Park, Hsinchu 30077, Taiwan
| | - Chun-Cheng Lin
- Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.
| |
Collapse
|
|
27
|
Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review. SENSORS 2017; 17:s17122952. [PMID: 29257113 PMCID: PMC5750823 DOI: 10.3390/s17122952] [Citation(s) in RCA: 64] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 10/27/2017] [Revised: 11/24/2017] [Accepted: 12/13/2017] [Indexed: 02/06/2023]
Abstract
Wound assessment is usually performed in hospitals or specialized labs. However, since patients spend most of their time at home, a remote real time wound monitoring would help providing a better care and improving the healing rate. This review describes the advances in sensors and biosensors for monitoring the concentration of C-reactive protein (CRP), temperature and pH in wounds. These three parameters can be used as qualitative biomarkers to assess the wound status and the effectiveness of therapy. CRP biosensors can be classified in: (a) field effect transistors, (b) optical immunosensors based on surface plasmon resonance, total internal reflection, fluorescence and chemiluminescence, (c) electrochemical sensors based on potentiometry, amperometry, and electrochemical impedance, and (d) piezoresistive sensors, such as quartz crystal microbalances and microcantilevers. The last section reports the most recent developments for wearable non-invasive temperature and pH sensors suitable for wound monitoring.
Collapse
|
|
28
|
Immunosensor assembled on polymeric nanostructures for clinical diagnosis of C-reactive protein. Microchem J 2017. [DOI: 10.1016/j.microc.2017.04.026] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
|
|
29
|
Chen G. Dynamic relationship between postoperative infection and CRP, IL-6, and Livin in patients with bone tumors. MINERVA CHIR 2017; 74:392-398. [PMID: 28233481 DOI: 10.23736/s0026-4733.17.07321-7] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/08/2022]
Abstract
BACKGROUND The objective of the present study was to investigate the dynamic relationship between postoperative infection and levels of CRP, IL-6, and Livin in patients with bone tumors. METHODS A total of 126 patients with bone tumors admitted to our hospital from November 2013 to October 2015 were randomly selected and retrospectively analyzed. According to whether postoperative infection occurred, patients were divided into the infected group (N.=52) and the non-infected group (N.=74). Before surgery, and on the 1st, 3rd, 5th, 7th, and 10th day after surgery, the levels of serum CRP and IL-6 of patients in both groups were compared; the relative expression levels of Livin protein of patients in both groups were measured by Western blot. RESULTS After surgery, the levels of CRP and IL-6 of patients in both groups at all time points were significantly higher than those before surgery and the levels of CRP and IL-6 of the infected group were significantly higher than those of the non-infected group (P<0.05). The levels of CRP and IL-6 reached a peak on the 3rd day after surgery, and CRP declined rapidly. The levels of IL-6 declined slowly. On the 10th day after surgery, the levels of IL-6 of patients in both groups were higher than those before surgery and the levels of IL-6 of patients in the infected group were higher than those of the control group (P<0.05). The levels of Livin protein in patients of both groups were not significantly different (P>0.05). CONCLUSIONS The increase of the levels of CRP and IL-6 are closely related to infection. Livin has no close relationship with the occurrence of infection.
Collapse
Affiliation(s)
- Ge Chen
- Department of Orthopedics, First Affiliated Hospital of South-West Medical University, Luzhou, China -
| |
Collapse
|
|
30
|
Bioanalytical advances in assays for C-reactive protein. Biotechnol Adv 2016; 34:272-90. [DOI: 10.1016/j.biotechadv.2015.12.010] [Citation(s) in RCA: 94] [Impact Index Per Article: 10.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2015] [Revised: 12/17/2015] [Accepted: 12/18/2015] [Indexed: 01/08/2023]
|
|
31
|
Label-free detection of C-reactive protein using an electrochemical DNA immunoassay. SENSING AND BIO-SENSING RESEARCH 2016. [DOI: 10.1016/j.sbsr.2016.03.003] [Citation(s) in RCA: 29] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
|
|
32
|
Abstract
In this paper, we propose an AlGaN/GaN high electron mobility transistor (HEMT)-based biosensor for the detection of C-reactive protein (CRP) using a null-balancing circuit. A null-balancing circuit was used to measure the output voltage of the sensor directly. The output voltage of the proposed biosensor was varied by antigen-antibody interactions on the gate surface due to CRP charges. The AlGaN/GaN HFET-based biosensor with null-balancing circuit applied shows that CRP can be detected in a wide range of concentrations, varying from 10 ng/mL to 1000 ng/mL. X-ray photoelectron spectroscopy was carried out to verify the immobilization of self-assembled monolayer with Au on the gated region.
Collapse
|
|
33
|
Huang W, Diallo AK, Dailey JL, Besar K, Katz HE. Electrochemical processes and mechanistic aspects of field-effect sensors for biomolecules. JOURNAL OF MATERIALS CHEMISTRY. C 2015; 3:6445-6470. [PMID: 29238595 PMCID: PMC5724786 DOI: 10.1039/c5tc00755k] [Citation(s) in RCA: 51] [Impact Index Per Article: 5.1] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/21/2023]
Abstract
Electronic biosensing is a leading technology for determining concentrations of biomolecules. In some cases, the presence of an analyte molecule induces a measured change in current flow, while in other cases, a new potential difference is established. In the particular case of a field effect biosensor, the potential difference is monitored as a change in conductance elsewhere in the device, such as across a film of an underlying semiconductor. Often, the mechanisms that lead to these responses are not specifically determined. Because improved understanding of these mechanisms will lead to improved performance, it is important to highlight those studies where various mechanistic possibilities are investigated. This review explores a range of possible mechanistic contributions to field-effect biosensor signals. First, we define the field-effect biosensor and the chemical interactions that lead to the field effect, followed by a section on theoretical and mechanistic background. We then discuss materials used in field-effect biosensors and approaches to improving signals from field-effect biosensors. We specifically cover the biomolecule interactions that produce local electric fields, structures and processes at interfaces between bioanalyte solutions and electronic materials, semiconductors used in biochemical sensors, dielectric layers used in top-gated sensors, and mechanisms for converting the surface voltage change to higher signal/noise outputs in circuits.
Collapse
Affiliation(s)
- Weiguo Huang
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, 206 Maryland Hall, Baltimore, MD, USA
| | - Abdou Karim Diallo
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, 206 Maryland Hall, Baltimore, MD, USA
| | - Jennifer L Dailey
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, 206 Maryland Hall, Baltimore, MD, USA
| | - Kalpana Besar
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, 206 Maryland Hall, Baltimore, MD, USA
| | - Howard E Katz
- Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, 206 Maryland Hall, Baltimore, MD, USA
| |
Collapse
|
|
34
|
Patil AV, Bedatty Fernandes FC, Bueno PR, Davis JJ. Immittance Electroanalysis in Diagnostics. Anal Chem 2014; 87:944-50. [DOI: 10.1021/ac503156a] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Affiliation(s)
- Amol V. Patil
- Department
of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom
| | - Flávio C. Bedatty Fernandes
- Institute
of Chemistry, Physical Chemistry Department, Univ. Estadual Paulista (São Paulo State University), Nanobionics Research Group, CP 355, 14800-900, Araraquara, São
Paulo, Brazil
| | - Paulo R. Bueno
- Institute
of Chemistry, Physical Chemistry Department, Univ. Estadual Paulista (São Paulo State University), Nanobionics Research Group, CP 355, 14800-900, Araraquara, São
Paulo, Brazil
| | - Jason J. Davis
- Department
of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom
| |
Collapse
|
|
35
|
Phurimsak C, Tarn MD, Peyman SA, Greenman J, Pamme N. On-Chip Determination of C-Reactive Protein Using Magnetic Particles in Continuous Flow. Anal Chem 2014; 86:10552-9. [DOI: 10.1021/ac5023265] [Citation(s) in RCA: 33] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Affiliation(s)
- Chayakom Phurimsak
- Department
of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
| | - Mark D. Tarn
- Department
of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
| | - Sally A. Peyman
- Department
of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
| | - John Greenman
- School
of Biological, Biomedical and Environmental Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
| | - Nicole Pamme
- Department
of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom
| |
Collapse
|
|
36
|
Abstract
Nanofluidics is generally described as the study of liquid flow in or around structures of 100 nm or smaller, and its use for lab on a chip devices has now been actively studied for two decades. Here a brief review is given of the impact that this nanofluidics research has had on point of care applications. Four areas are identified where nanofluidics has brought the largest contributions: single nanopores, nanoporous membranes, nanoconfinement and the use of concentration polarization. The sometimes revolutionary developments in these areas are briefly treated and finally challenges and future perspectives are described.
Collapse
Affiliation(s)
- L I Segerink
- BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, University of Twente, Enschede, the Netherlands.
| | | |
Collapse
|
|
37
|
Kim JY, Ahn JH, Moon DI, Park TJ, Lee SY, Choi YK. Multiplex electrical detection of avian influenza and human immunodeficiency virus with an underlap-embedded silicon nanowire field-effect transistor. Biosens Bioelectron 2014; 55:162-7. [DOI: 10.1016/j.bios.2013.12.014] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/02/2013] [Revised: 11/26/2013] [Accepted: 12/05/2013] [Indexed: 10/25/2022]
|
|
38
|
Chandra P, Suman P, Airon H, Mukherjee M, Kumar P. Prospects and advancements in C-reactive protein detection. World J Methodol 2014; 4:1-5. [PMID: 25237625 PMCID: PMC4145575 DOI: 10.5662/wjm.v4.i1.1] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/18/2013] [Revised: 01/13/2014] [Accepted: 02/18/2014] [Indexed: 02/06/2023] Open
Abstract
C-reactive protein (CRP) is one of the earliest proteins that appear in the blood circulation in most systemic inflammatory conditions and this is the reason for its significance, even after identification of many organ specific inflammatory markers which appear relatively late during the course of disease. Earlier methods of CRP detection were based on the classical methods of antigen-antibody interaction through precipitation and agglutination reactions. Later on, CRP based enzymatic assays came into the picture which were further modified by integration of an antigen-antibody detection system with surface plasma spectroscopy. Then came the time for the development of electrochemical biosensors where nanomaterials were used to make a highly sensitive and portable detection system based on silicon nanowire, metal-oxide-semiconductor field-effect transistor/bipolar junction transistor, ZnS nanoparticle, aptamer, field emission transmitter, vertical flow immunoassay etc. This editorial attempts to summarize developments in the field of CRP detection, with a special emphasis on biosensor technology. This would help in translating the latest development in CRP detection in the clinical diagnosis of inflammatory conditions at an early onset of the diseases.
Collapse
|
|
39
|
Hasanzadeh M, Shadjou N, Soleymani J, Omidinia E, de la Guardia M. Optical immunosensing of effective cardiac biomarkers on acute myocardial infarction. Trends Analyt Chem 2013. [DOI: 10.1016/j.trac.2013.06.010] [Citation(s) in RCA: 26] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2023]
|