Constitutive renal Rel/nuclear factor-κB expression in Lewis polycystic kidney disease rats

Table 1 Histological analysis of Lewis and Lewis polycystic kidney rats from weeks 1 to 20

Parameter		Week 1	Week 3	Week 6	Week 10	Week 16	Week 20
Cyst length (μm)	Lewis	ND	ND	ND	ND	ND	ND
	LPK	106 ± 4	347 ± 88	938 ± 280	1548 ± 237	2252 ± 436	3718 ± 922
Cyst diameter (μm)	Lewis	ND	ND	ND	ND	ND	ND
	LPK	69 ± 18	101 ± 19	279 ± 71	442 ± 81	681 ± 121	1119 ± 256
Ki-67 (%)	Lewis	24.3 + 8.3	1.9 + 0.6	2.4 + 1.5	0.2 + 0.1	0.3 + 0.1	0.2 ± 0.02
	LPK	7.0 ± 3.8a	9.9 + 2.6a	2.2 ± 1.5	1.1 ± 0.4a	1.9 ± 0.8a	0.8 ± 0.3a
ED-1 (%)	Lewis	1.87 ± 0.61	1.26 ± 0.20	1.32 ± 0.95	0.56 ± 0.08	0.22 ± 0.11	0.16 ± 0.02
	LPK	1.58 ± 0.15	3.81 ± 1.02a	4.87 ± 1.21a	12.37 ± 4.10a	0.81 ± 0.38a	0.84 ± 0.24a
Sirius Red (%)	Lewis	0.86 ± 0.38	4.32 ± 1.58	1.67 ± 0.52	2.91 ± 0.77	0.51 ± 0.43	0.64 ± 0.40
	LPK	1.96 ± 1.15	2.88 ± 1.51a	5.92 ± 4.20b	16.63 ± 8.12b	10.34 ± 5.82b	18.24 ± 6.00b
α-SMA (%)	Lewis	17.2 ± 2.12	1.68 ± 0.42	1.87 ± 0.32	0.98 ± 0.86	1.04 ± 0.06	1.09 ± 0.13
	LPK	22.4 ± 2.80	8.70 ± 3.70a	8.98 ± 3.41a	20.18 ± 3.54a	13.75 ± 0.99a	13.28 ± 1.68a

Data expressed as mean ± SD;

^aP < 0.05 vs age-matched Lewis male animals;

^bP < 0.01 vs age-matched Lewis male animals. α-SMA: Alpha-smooth muscle actin; BrdU: 5-Bromo-2’-deoxyuridine; ND: Not determined.

Table 2 Renal function for male Lewis and Lewis polycystic kidney rats from weeks 3 to 20

Parameter		Week 3	Week 6	Week 10	Week 16	Week 20
24 h urine volume (mL)	Lewis	ND	6 ± 1	9 ± 2	9 ± 2	ND
	LPK	ND	7 ± 1	25 ± 2b	23 ± 9b	ND
Serum creatinine (μmol/L)	Lewis	24 ± 3	22 ± 5	29 ± 10	24 ± 1	27 ± 5
	LPK	20 ± 2a	28 ± 14	33 ± 3b	63 ± 9	191 ± 38b
Endogenous CrCl (mL/min)	Lewis	ND	0.5 ± 0.2	1.8 ± 0.3	2.5 ± 0.6	ND
	LPK	ND	0.5 ± 0.2	0.8 ± 0.1b	0.6 ± 0.2b	ND
CrCl/BW (fold-change over Lewis)	Lewis	ND	1 ± 0.4	1.6 ± 0.1	1.7 ± 0.3	ND
	LPK	ND	0.9 ± 0.3	0.8 ± 0.1b	0.4 ± 0.1b	ND
Serum urea (mmol/L)	Lewis	5 ± 1	6 ± 1	6 ± 1	6 ± 1	8 ± 1
	LPK	7 ± 0b	8 ± 1b	14 ± 1b	22 ± 3b	44 ± 4b

Data expressed as mean ± SD;

^aP < 0.05 vs age-matched Lewis male animals;

^bP < 0.01 vs age-matched Lewis male animals. BW: Body weight; CrCl: Creatinine clearance; ND: Not determined.