High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay

Table 1 List of high-throughput RNA interference screens performed to identify host factors involved in viral life cycle

Family	Virus	Readout	Viral life cycle step(s) studied	Partition (primary/validation)
Flaviviridae (ssRNA+)	DENV1[100]	IF	Single step	Primary: single oligo; validation, single oligo (the same)
Picornavirus (ssRNA+)	DCV1[22]	IF	Single step	Primary: single, validation, single (the same)
Flaviviridae (ssRNA+)	HCV[21]	Luc (reporter virus)	Single step (replicon)	Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually
Flaviviridae (ssRNA+)	HCV[20]	IF	Two step (full virus): entry/ replication; assembly/release	Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually
Retroviridae (dsRNA)	HIV-1[17]	Part I: IF; part II: Luc (reporter cell line)	Two steps: entry/replication; assembly/release	Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually
Retroviridae (dsRNA)	HIV-1[18]	Luc (reporter virus)	Single step	Primary only: 3 pools of 2
Retroviridae (dsRNA)	HIV-1[19]	Beta-Gal activity	Two steps, but without distinction between them	Primary: pools of 3; validation; independent poools of 3
Orthomixoviridae; (ssRNA- segmented)	INF[14]	Part I: IF; part II: Luc (reporter cell line)	Two steps: entry/replication; assembly/release	Primary: 4 oligoes
Orthomixoviridae; (ssRNA- segmented)	INF[15]	Luc (reporter virus)	Single step	Primary only: some genes 2 oligoes, other genes only one
Orthomixoviridae; (ssRNA- segmented)	INF1[16]	Luc (reporter virus)	Single step	Primary only: a single sirna x gene
Rhabdoviridae (ssRNA-)	VSV[29]	IF	Single step	Primary: 2 pools of 2 oligos; validation: pool of 4 oligos from different vendor
Flaviviridae (ssRNA+)	WNV[28]	IF	Single step	Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually

¹Drosophila cells. HIV: Human immunodeficiency virus; IF: Immunofluorescence; Luc: Luciferase; DENV: Dengue virus; DCV: Drosophila C virus; HCV: Hepatitis C virus; VSV: Vesicular stomatitis virus; WNV: West Nile virus.