Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation

Table 1 Donor specific antibodies solid phase single-antigen Luminex analysis in unmodified and Dithiothreitol treated serum

Serum treatment	HLA specificities
	B8	B18	DR53
DDT treated	247771	23500	23100
Untreated	550	1764	4852

¹The numbers in the table indicate MFI values. HLA: Human leukocyte antigen; MFI: Mean fluorescence intensity; DDT: Dithiothreitol.

Table 2 Epitope analysis of human leukocyte antigen recipient developed antibodies to “antigens”

HLA	MFI	Reactive eplets/pair of eplets	Number of mismatched eplets	Mismatched eplets	Comments
B*07:02a	1689	44re	5	44reb, 65qia, 70iaq, 113hd, 177dk	44re + self 69at
B*45:01a	1433	167es	1	167esb
B*44:03	1398	167es	5	76ent, 79rt, 94ii, 167es, 199v
B*82:01	1077	44re, 167es	4	44re, 65qia, 70iaq, 167es
B*44:02	841	167es	5	76ent, 79rt, 94ii, 167es, 199v
C*07:02	657	44re, 167es	5	44re, 65qia, 70iaq, 94ii, 167es
B*81:01	1222	44re	4	44re, 65qia, 70iaq, 254ta	44re + self 69at
B*27:08	1130	44re	3	44re, 65qia, 71ka	44re + self 69at
B*56:01	1059	44re	3	44re, 65qia, 71ka	44re + self 69at
B*55:01	837	44re	3	44re, 65qia, 71ka	44re + self 69at
B*42:01	761	44re	3	44re, 65qia, 71ka	44re + self 69at
B*67:01	681	44re	4	44re, 65qia, 70iaq, 158t	44re + self 69at
B*27:05	607	44re	5	44re, 65qia, 71ka, 76 edt, 79rt	44re + self 69at
B*73:01	327	44re	6	44re, 65qia, 71ka, 103 m, 267qe, 275kp	44re + self 69at
C*06:02a	379	44re, 167es	5	44re, 65qia, 70iaq, 94ii, 167es
B*08:01	2046	44re	1	44r	44re + 69tn
B*15:12 (B76)	1294	NDc	3	45rma, 113hd, 163lg	Unexplained

^aAlleles in bold font are mismatches with the 1^st donor;

^bReactive eplets;

^cNot determined. HLA: Human leukocyte antigen; MFI: Mean fluorescence intensity.

Table 3 Human leukocyte antigen Class I typing results of the recipient and previous and current donors

	A locus		B locus		C locus
Recipient	23:01	66:01	41:01	49:01	07:01	17:01
1st donor (immunizer)	03:01a	26:01	07:02	45:01	06:02	07:01
Current donor	23:01	24:01	44:02	49:01	03:03	07:01

^aMismatched alleles are given in bold font.