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1
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Li X, Fu L, Chen F, Lv Y, Zhang R, Zhao S, Karimi-Maleh H. Cyclodextrin-based architectures for electrochemical sensing: from molecular recognition to functional hybrids. ANALYTICAL METHODS : ADVANCING METHODS AND APPLICATIONS 2025. [PMID: 40392560 DOI: 10.1039/d5ay00612k] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/22/2025]
Abstract
This review surveys recent advances in the integration of cyclodextrins (CDs) with diverse materials for electrochemical detection of a wide range of analytes in environmental, pharmaceutical, and clinical contexts. CDs, featuring a hydrophobic cavity and a hydrophilic exterior, enable selective host-guest binding of small organic and inorganic molecules. By anchoring CDs onto electrode surfaces via strategies such as self-assembled monolayers, layer-by-layer deposition, or polymer entrapment, researchers have achieved improved selectivity and lower detection limits for target compounds. These CD-functionalized interfaces are further enhanced by combination with carbon nanotubes, graphene, metal nanoparticles, and redox mediators, providing synergistic effects that boost conductivity, catalysis, and signal amplification. Moreover, CD-based sensors exhibit reversible recognition, making them amenable to repeated use and continuous monitoring. Notably, derivatization of the CD ring expands its applicability, introducing functionalities such as chirality recognition, metal coordination, or improved solubility. Different detection modes, including voltammetry, impedance, and competitive displacement assays, have been reported for a variety of analytes, ranging from heavy metals and pesticides to pharmaceuticals and chiral compounds. The incorporation of CDs into advanced hybrid architectures also offers solutions to common issues like electrode fouling and limited selectivity, thus expanding their utility in harsh or complex sample environments. While challenges remain in ensuring reproducibility, large-scale manufacture, and robust performance in real-world applications, ongoing innovations in materials science and synthetic chemistry promise to make CD-based electrodes increasingly valuable for sensitive, portable, and cost-effective chemical analysis. Furthermore, novel integration with biological receptors, such as enzymes and aptamers, holds promise for multiplexed biosensing.
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Affiliation(s)
- Xingxing Li
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Li Fu
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Fei Chen
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Yanfei Lv
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Rui Zhang
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Shichao Zhao
- College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
| | - Hassan Karimi-Maleh
- The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, China
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2
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AbhijnaKrishna R, Lu YH, Wu SP, Velmathi S. Comparative Study of Fluorophores for Precise Dopamine Detection and Investigation of Its Association with Stress and Coffee Addiction in HEK 293 Cells. ACS APPLIED BIO MATERIALS 2025. [PMID: 40019175 DOI: 10.1021/acsabm.5c00039] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/01/2025]
Abstract
In today's world, where stress and addiction are increasingly prevalent due to job pressures and coping mechanisms, dopamine (Dopa), a key hormone linked to mood, happiness, and mental health, has become vital for understanding conditions like depression and anxiety. Our study focuses on detecting Dopa pathways both in vitro using HEK293 cells and in vivo using zebrafish under stress and addiction conditions. We employed a biocompatible organic fluorophore (P1), with pyrazole-4-carboxaldehyde as the recognition unit, which demonstrated a detection limit of 8.2 nM, aligning with physiological Dopa levels. P1's efficacy in detecting Dopa was validated in human samples (urine, blood, and serum) and artificial samples, confirming its potential for real-world applications. This research is crucial for developing better diagnostic tools and therapies for dopamine-related disorders, offering significant societal benefits for addressing mental health challenges.
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Affiliation(s)
| | - Yueh-Hsun Lu
- Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
| | - Shu-Pao Wu
- Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
| | - Sivan Velmathi
- Department of Chemistry, National Institute of Technology Tiruchirappalli, Tamil Nadu 620015, India
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3
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López JG, Muñoz M, Arias V, García V, Calvo PC, Ondo-Méndez AO, Rodríguez-Burbano DC, Fonthal F. Electrochemical and Optical Carbon Dots and Glassy Carbon Biosensors: A Review on Their Development and Applications in Early Cancer Detection. MICROMACHINES 2025; 16:139. [PMID: 40047624 PMCID: PMC11857277 DOI: 10.3390/mi16020139] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 12/18/2024] [Revised: 01/22/2025] [Accepted: 01/23/2025] [Indexed: 03/09/2025]
Abstract
Cancer remains one of the leading causes of mortality worldwide, making early detection a critical factor in improving patient outcomes and survival rates. Developing advanced biosensors is essential for achieving early detection and accurate cancer diagnosis. This review offers a comprehensive overview of the development and application of carbon dots (CDs) and glassy carbon (GC) biosensors for early cancer detection. It covers the synthesis of CDs and GC, electrode fabrication methods, and electrochemical and optical transduction principles. This review explores various biosensors, including enzymatic and non-enzymatic, and discusses key biomarkers relevant to cancer detection. It also examines characterization techniques for electrochemical and optical biosensors, such as electrochemical impedance spectroscopy, cyclic voltammetry, UV-VIS, and confocal microscopy. The findings highlight the advancements in biosensor performance, emphasizing improvements in sensitivity, selectivity, and stability, as well as underscoring the potential of integrating different transduction methods and characterization approaches to enhance early cancer detection.
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Affiliation(s)
- Juana G. López
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
| | - Mariana Muñoz
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
| | - Valentina Arias
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
| | - Valentina García
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
| | - Paulo C. Calvo
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
| | - Alejandro O. Ondo-Méndez
- Clinical Investigation Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá 111221, Colombia;
| | - Diana C. Rodríguez-Burbano
- Givia Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá 111221, Colombia;
| | - Faruk Fonthal
- Biomedical Engineering Research Group—GBIO, Universidad Autónoma de Occidente, Cali 760030, Colombia; (J.G.L.); (M.M.); (V.A.); (V.G.); (P.C.C.)
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4
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Vural O, Buğday N, Genc AA, Erk N, Duygulu O, Yaşar S. Superior Electrochemical Sensor Application of Co 3O 4/C Heterostructure in Rapid Analysis of Anticancer Drug Palbociclib in Pharmaceutical Formulations and Biological Fluids. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2024; 40:21139-21151. [PMID: 39340460 PMCID: PMC11465771 DOI: 10.1021/acs.langmuir.4c02551] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/05/2024] [Revised: 09/14/2024] [Accepted: 09/18/2024] [Indexed: 09/30/2024]
Abstract
In this work, we report a study examining how different salt concentrations affect the structure and electrochemical performance of two Co3O4/C materials designed for the fabrication of an easy, cheap, fast, safe, and useful electrochemical sensor for the detection of Palbociclib (PLB). Co3O4 nanoparticles were successfully created by encapsulating them in N-doped amorphous carbon matrices by using the molten salt-assisted approach. In this process, different amounts of potassium iodate and zeolitic imidazolate framework-12 (ZIF-12) were used, followed by pyrolysis at 800 °C. Optimum Co3O4 embedded porous carbon structures were obtained, and the composite with the highest electrochemical properties was modified to a glassy carbon electrode (GCE) surface for PLB detection. The linear response spanned from 1.0 to 5.0 μM, featuring a limit of detection (LOD) of 0.122 μM and a limit of quantification (LOQ) of 0.408 μM; the correlation coefficient was calculated as 0.995. The high sensitivity of the method in detecting PLB in pharmaceutical samples and human urine demonstrated its feasibility, with recovery percentages ranging from 99.3% to 101.3% and relative standard deviation (RSD) values of <3%. Therefore, this technique will make a significant contribution to monitoring and improving existing cancer treatment options.
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Affiliation(s)
- Ozgul Vural
- Faculty
of Pharmacy, Department of Analytical Chemistry, Ankara University, 06560 Ankara, Turkey
- The
Graduate School of the Health Sciences, Ankara University, 06110 Ankara, Turkey
| | - Nesrin Buğday
- Faculty
of Science and Art, Department of Chemistry, İnönü Üniversity, 44280 Malatya, Turkey
| | - Asena Ayşe Genc
- Faculty
of Pharmacy, Department of Analytical Chemistry, Ankara University, 06560 Ankara, Turkey
- The
Graduate School of the Health Sciences, Ankara University, 06110 Ankara, Turkey
| | - Nevin Erk
- Faculty
of Pharmacy, Department of Analytical Chemistry, Ankara University, 06560 Ankara, Turkey
| | - Ozgur Duygulu
- TUBITAK
Marmara Research Center, Materials Technologies, 41470 Gebze, Kocaeli, Turkey
| | - Sedat Yaşar
- Faculty
of Science and Art, Department of Chemistry, İnönü Üniversity, 44280 Malatya, Turkey
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5
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Merli D, Cutaia A, Hallulli I, Bonanni A, Alberti G. Molecularly Imprinted Polypyrrole-Modified Screen-Printed Electrode for Dopamine Determination. Polymers (Basel) 2024; 16:2528. [PMID: 39274160 PMCID: PMC11397747 DOI: 10.3390/polym16172528] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2024] [Revised: 09/01/2024] [Accepted: 09/05/2024] [Indexed: 09/16/2024] Open
Abstract
This paper introduces a quantitative method for dopamine determination. The method is based on a molecularly imprinted polypyrrole (e-MIP)-modified screen-printed electrode, with differential pulse voltammetry (DPV) as the chosen measurement technique. The dopamine molecules are efficiently entrapped in the polymeric film, creating recognition cavities. A comparison with bare and non-imprinted polypyrrole-modified electrodes clearly demonstrates the superior sensitivity, selectivity, and reproducibility of the e-MIP-based one; indeed, a sensitivity of 0.078 µA µM-1, a detection limit (LOD) of 0.8 µM, a linear range between 0.8 and 45 µM and a dynamic range of up to 350 µM are achieved. The method was successfully tested on fortified synthetic and human urine samples to underline its applicability as a screening method for biomedical tests.
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Affiliation(s)
- Daniele Merli
- Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
| | - Alessandra Cutaia
- Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
| | - Ines Hallulli
- Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
| | - Alessandra Bonanni
- Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
| | - Giancarla Alberti
- Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
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6
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Bhattacharjya R, Kalita S, Dutta A, Basak D, Saikia H. Selective and Comparative Study of B/nZVCu-Fe and B/nZVCu-Zn Nanoparticles as Fluorescent Probe for Dopamine in Presence of its Interference Molecules. J Fluoresc 2024:10.1007/s10895-024-03873-9. [PMID: 39180575 DOI: 10.1007/s10895-024-03873-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/13/2024] [Accepted: 07/29/2024] [Indexed: 08/26/2024]
Abstract
This work focuses on the synthesis of Bentonite supported nano zero valent bimetallic nanoparticles (B/nZVCu-M NPs) to be utilized for fast and highly sensitive, reversible, fluorescent determination of dopamine (DA) in the presence of dopamine, other biomolecules and ions. The X-ray Photoelectron Spectroscopy(XPS), Powder X-Ray Diffraction(PXRD) and Scanning Electron Microscopy(SEM) revealed the formation of nanoparticles with size ranging from 15 to 20 nm. The composition was revealed by Fourier Transform Infrared(FTIR) Spectoscopy and Energy Dispersive X-Ray (EDX) Analysis. The Limits of Detection(LOD) were noted to be 5.57nM and 6.07nM. The binding of DA is noted to be reversible with respect to EDTA2-. Furthermore, the developed sensor exhibited good repeatability, satisfactory long-term stability, and was successfully used for the selective detection of dopamine sample with desired recoveries or reversibilities. The main aim of our work is to selectively detect dopamine in presence of its major interferents and biomolecules that are normally present/ co-exist with dopamine in biological systems.
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Affiliation(s)
| | - Sarojmoni Kalita
- Department of Chemistry, Gauhati University, Assam, 781014, India
| | - Ananya Dutta
- Department of Chemistry, Gauhati University, Assam, 781014, India
| | - Dipanwita Basak
- Department of Chemistry, Bodoland University, Kokrajhar, Assam, 783370, India
| | - Hemaprobha Saikia
- Department of Chemistry, Bodoland University, Kokrajhar, Assam, 783370, India.
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Li C, He Y, Ingebrandt S, Vu XT. Microscale Sensor Arrays for the Detection of Dopamine Using PEDOT:PSS Organic Electrochemical Transistors. SENSORS (BASEL, SWITZERLAND) 2024; 24:5244. [PMID: 39204939 PMCID: PMC11360330 DOI: 10.3390/s24165244] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 07/03/2024] [Revised: 07/24/2024] [Accepted: 08/10/2024] [Indexed: 09/04/2024]
Abstract
We present a sensor array of microscale organic electrochemical transistors (OECTs) using poly (3,4-ethylenedioxythiophene):poly(styrenesulfonic acid) (PEDOT:PSS) as the channel material. The devices show high sensitivity and selectivity to detect dopamine (DA) with platinum (Pt) as a pseudo-reference gate electrode. First, we describe the wafer-scale fabrication process for manufacturing the PEDOT:PSS OECTs, and then we introduce a dilution method to adjust the thickness of the PEDOT:PSS film. Next, we investigate the effect of the film thickness on the sensitivity of DA detection. Reducing the film thickness enhances the sensitivity of DA detection within the concentration range of 1 μM to 100 μM. The OECTs show impressive sensitivitywith a limit of detection (LoD) as low as 1 nM and a high selectivity against uric acid (UA) and ascorbic acid (AA). Finally, we modify the surface of the Pt gate electrode with chitosan to improve the selectivity of OECTs at high concentrations of up to 100 µM to expand the detection range.
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Affiliation(s)
| | | | | | - Xuan Thang Vu
- Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Sommerfeldstr. 24, 52074 Aachen, Germany; (C.L.); (Y.H.); (S.I.)
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8
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Puglisi R, Cavallaro A, Pappalardo A, Petroselli M, Santonocito R, Trusso Sfrazzetto G. A New BODIPY-Based Receptor for the Fluorescent Sensing of Catecholamines. Molecules 2024; 29:3714. [PMID: 39125116 PMCID: PMC11314322 DOI: 10.3390/molecules29153714] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/10/2024] [Revised: 07/31/2024] [Accepted: 08/01/2024] [Indexed: 08/12/2024] Open
Abstract
The human body synthesizes catecholamine neurotransmitters, such as dopamine and noradrenaline. Monitoring the levels of these molecules is crucial for the prevention of important diseases, such as Alzheimer's, schizophrenia, Parkinson's, Huntington's, attention-deficit hyperactivity disorder, and paragangliomas. Here, we have synthesized, characterized, and functionalized the BODIPY core with picolylamine (BDPy-pico) in order to create a sensor capable of detecting these biomarkers. The sensing properties of the BDPy-pico probe in solution were studied using fluorescence titrations and supported by DFT studies. Catecholamine sensing was also performed in the solid state by a simple strip test, using an optical fiber as the detector of emissions. In addition, the selectivity and recovery of the sensor were assessed, suggesting the possibility of using this receptor to detect dopamine and norepinephrine in human saliva.
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Affiliation(s)
- Roberta Puglisi
- Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy; (R.P.); (A.C.); (A.P.)
| | - Alessia Cavallaro
- Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy; (R.P.); (A.C.); (A.P.)
| | - Andrea Pappalardo
- Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy; (R.P.); (A.C.); (A.P.)
- Research Unit of Catania, National Interuniversity Consortium for Materials Science and Technology (I.N.S.T.M.), Viale Andrea Doria 6, 95125 Catania, Italy
| | - Manuel Petroselli
- Institute of Chemical Research of Catalonia (ICIQ), Av. Països Catalans 16, 43007 Tarragona, Spain;
| | - Rossella Santonocito
- Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy; (R.P.); (A.C.); (A.P.)
| | - Giuseppe Trusso Sfrazzetto
- Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy; (R.P.); (A.C.); (A.P.)
- Research Unit of Catania, National Interuniversity Consortium for Materials Science and Technology (I.N.S.T.M.), Viale Andrea Doria 6, 95125 Catania, Italy
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9
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Spitz S, Schobesberger S, Brandauer K, Ertl P. Sensor-integrated brain-on-a-chip platforms: Improving the predictive validity in neurodegenerative research. Bioeng Transl Med 2024; 9:e10604. [PMID: 38818126 PMCID: PMC11135156 DOI: 10.1002/btm2.10604] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2023] [Revised: 08/31/2023] [Accepted: 09/12/2023] [Indexed: 06/01/2024] Open
Abstract
Affecting millions of individuals worldwide, neurodegenerative diseases (NDDs) pose a significant and growing health concern in people over the age of 60 years. Contributing to this trend are the steady increase in the aging population coupled with a persistent lack of disease-altering treatment strategies targeting NDDs. The absence of efficient therapeutics can be attributed to high failure rates in clinical trials and the ineptness of animal models in preceding preclinical studies. To that end, in recent years, significant research effort has been dedicated to the development of human cell-based preclinical disease models characterized by a higher degree of predictive validity. However, a key requirement of any in vitro model constitutes the precise knowledge and replication of the target tissues' (patho-)physiological microenvironment. Herein, microphysiological systems have demonstrated superiority over conventional static 2D/3D in vitro cell culture systems, as they allow for the emulation and continuous monitoring of the onset, progression, and remission of disease-associated phenotypes. This review provides an overview of recent advances in the field of NDD research using organ-on-a-chip platforms. Specific focus is directed toward non-invasive sensing strategies encompassing electrical, electrochemical, and optical sensors. Additionally, promising on- and integrable off-chip sensing strategies targeting key analytes in NDDs will be presented and discussed in detail.
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Affiliation(s)
- Sarah Spitz
- Faculty of Technical ChemistryVienna University of TechnologyViennaAustria
- Present address:
Department of Mechanical Engineering and Biological EngineeringMassachusetts Institute of TechnologyCambridgeMassachusettsUSA
| | | | | | - Peter Ertl
- Faculty of Technical ChemistryVienna University of TechnologyViennaAustria
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10
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Hemmerová E, Homola J. Combining plasmonic and electrochemical biosensing methods. Biosens Bioelectron 2024; 251:116098. [PMID: 38359667 DOI: 10.1016/j.bios.2024.116098] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/15/2023] [Revised: 01/29/2024] [Accepted: 01/31/2024] [Indexed: 02/17/2024]
Abstract
The idea of combining electrochemical (EC) and plasmonic biosensor methods was introduced almost thirty years ago and the potential of electrochemical-plasmonic (EC-P) biosensors has been highlighted ever since. Despite that, the use of EC-P biosensors in analytics has been rather limited so far and the search for unique applications of the EC-P method continues. In this paper, we review the advances in the field of EC-P biosensors and discuss the features and benefits they can provide. In addition, we identify the main challenges for the development of EC-P biosensors and the limitations that prevent EC-P biosensors from more widespread use. Finally, we review applications of EC-P biosensors for the investigation and quantification of biomolecules, and for the study of biomolecular and cellular processes.
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Affiliation(s)
- Erika Hemmerová
- Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 1014/57, 182 51, Prague, Czech Republic
| | - Jiří Homola
- Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 1014/57, 182 51, Prague, Czech Republic.
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11
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Li T, Zhang J, Bu P, Wu H, Guo J, Guo J. Multi-modal nanoprobe-enabled biosensing platforms: a critical review. NANOSCALE 2024; 16:3784-3816. [PMID: 38323860 DOI: 10.1039/d3nr03726f] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/08/2024]
Abstract
Nanomaterials show great potential for applications in biosensing due to their unique physical, chemical, and biological properties. However, the single-modal signal sensing mechanism greatly limits the development of single-modal nanoprobes and their related sensors. Multi-modal nanoprobes can realize the output of fluorescence, colorimetric, electrochemical, and magnetic signals through composite nanomaterials, which can effectively compensate for the defects of single-modal nanoprobes. Following the multi-modal nanoprobes, multi-modal biosensors break through the performance limitation of the current single-modal signal and realize multi-modal signal reading. Herein, the current status and classification of multi-modal nanoprobes are provided. Moreover, the multi-modal signal sensing mechanisms and the working principle of multi-modal biosensing platforms are discussed in detail. We also focus on the applications in pharmaceutical detection, food and environmental fields. Finally, we highlight this field's challenges and development prospects to create potential enlightenment.
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Affiliation(s)
- Tong Li
- Chengdu University of Traditional Chinese Medicine, Chengdu, China
| | - Jiani Zhang
- Chengdu University of Traditional Chinese Medicine, Chengdu, China
| | - Pengzhi Bu
- Chengdu University of Traditional Chinese Medicine, Chengdu, China
| | - Haoping Wu
- Chengdu University of Traditional Chinese Medicine, Chengdu, China
| | - Jiuchuan Guo
- University of Electronic Science and Technology of China, Chengdu, China.
| | - Jinhong Guo
- School of Sensing Science and Engineering, Shanghai Jiao Tong, University, Shanghai, China.
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12
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Iftikhar T, Iftikhar N, Chi G, Qiu W, Xie Y, Liang Z, Huang C, Su L. Unlocking the future of brain research: MOFs, TMOs, and MOFs/TMOs for electrochemical NTMs detection and analysis. Talanta 2024; 267:125146. [PMID: 37688896 DOI: 10.1016/j.talanta.2023.125146] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/30/2023] [Revised: 08/29/2023] [Accepted: 08/31/2023] [Indexed: 09/11/2023]
Abstract
The central nervous system relies heavily on neurotransmitters (NTMs), and NTM imbalances have been linked to a wide range of neurological conditions. Thus, the development of reliable detection techniques is essential for advancing brain studies. This review offers a comprehensive analysis of metal-organic frameworks (MOFs), transition metal oxides (TMOs), and MOFs-derived TMOs (MOFs/TMOs) as materials for electrochemical (EC) sensors targeting the detection of key NTMs, specifically dopamine (DA), epinephrine (EP), and serotonin (SR). The unique properties and diverse families of MOFs and TMOs, along with their nanostructured hybrids, are discussed in the context of EC sensing. The review also addresses the challenges in detecting NTMs and proposes a systematic approach to tackle these obstacles. Despite the vast amount of research on MOFs and TMOs-based EC sensors for DA detection, the review highlights the gaps in the literature for MOFs/TMOs-based EC sensors specifically for EP and SR detection, as well as the limited research on microneedles (MNs)-based EC sensors modified with MOFs, TMOs, and MOFs/TMOs for NTMs detection. This review serves as a foundation to encourage researchers to further explore the potential applications of MOFs, TMOs, and MOFs/TMOs-based EC sensors in the context of neurological disorders and other health conditions related to NTMs imbalances.
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Affiliation(s)
- Tayyaba Iftikhar
- School of Biomedical Engineering, International Health Science Innovation Center, Shenzhen Key Laboratory of Nano-Biosensing Technology, Marshall Laboratory of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518055, PR China
| | - Nishwa Iftikhar
- Department of Medicine, Quaid-e-Azam Medical College, Bahawalpur, Punjab, Pakistan
| | - Guilin Chi
- School of Biomedical Engineering, International Health Science Innovation Center, Shenzhen Key Laboratory of Nano-Biosensing Technology, Marshall Laboratory of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518055, PR China
| | - Wenjing Qiu
- Department of Rheumatology, South China Hospital, Medical School, Shenzhen University, Shenzhen, 518116, PR China
| | - Yuanting Xie
- School of Biomedical Engineering, International Health Science Innovation Center, Shenzhen Key Laboratory of Nano-Biosensing Technology, Marshall Laboratory of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518055, PR China.
| | - Zhen Liang
- School of Biomedical Engineering, International Health Science Innovation Center, Shenzhen Key Laboratory of Nano-Biosensing Technology, Marshall Laboratory of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518055, PR China
| | - Cibo Huang
- Department of Rheumatology, South China Hospital, Medical School, Shenzhen University, Shenzhen, 518116, PR China
| | - Lei Su
- School of Biomedical Engineering, International Health Science Innovation Center, Shenzhen Key Laboratory of Nano-Biosensing Technology, Marshall Laboratory of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518055, PR China.
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Park JO, Choi Y, Ahn HM, Lee CK, Chun H, Park YM, Kim KB. Aggregation of Ag nanoparticle based on surface acoustic wave for surface-enhanced Raman spectroscopy detection of dopamine. Anal Chim Acta 2024; 1285:342036. [PMID: 38057052 DOI: 10.1016/j.aca.2023.342036] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/28/2023] [Revised: 10/13/2023] [Accepted: 11/15/2023] [Indexed: 12/08/2023]
Abstract
BACKGROUND Dopamine (DA), a vital neurotransmitter, plays a critical role in the human brain and relates to neuropsychiatric disorders such as Parkinson's disease and schizophrenia. Numerous studies have explored detection of such biomarkers through surface-enhanced Raman spectroscopy (SERS). However, most of the studies focused on SERS detection face significant challenges with plasmonic nanostructure development. Such challenges often include time-consuming processes, complex fabrication, specialized chemical labeling, poor reproducibility, and random hotspot generation. Therefore, the need for simple and rapid nanostructure development is evident in SERS. RESULTS We propose an innovative SERS-active sensing technique for 50 nm silver nanoparticle (AgNP) clustering based on surface acoustic wave (SAW). When a 1 μL droplet of AgNP colloid is dispensed onto the SAW-propagation zone, the AgNP cluster is deposited after the droplet completely evaporates, developing plasmonic nanogaps for SERS hotspot caused by spherical AgNP aggregation. By optimizing the SAW system through the hydrophobic treatment and modulation of the operational power, the SAW-induced AgNP clustering showed densely packed AgNP within a dot-like configuration (∼2200 AgNP μm-2), effectively preventing particle welding. The characterization of 4-mercaptobenzoic acid as a probe analyte revealed that concentrations as low as 1.14 pM was detected using our SAW-SERS system under 785 nm laser excitation. Moreover, DA was detected up to 4.28 nM with a determination of 0.99 (R2). SIGNIFICANCE This technique for AgNP clustering induced by SAW provides a rapid, in situ, label-free SERS sensing method with outstanding sensitivity and linearity. A mere act of dropping can create extensive plasmonic hotspots featuring nanogap of ∼1.5 nm. The SAW-induced AgNP clustering can serve as an ultrasensitive SERS-active substrate for diverse molecular detections, including neurotransmitter detection.
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Affiliation(s)
- Jin Oh Park
- Digital Health Care R&D Department, Korea Institute of Industrial Technology (KITECH), 89, Yangdaegiro-gil, Ipjang-myeon, Seobuk-gu, Cheonan, 31056, Republic of Korea; Department of Biomedical Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea
| | - Yongheum Choi
- Heat and Surface Technology R&D Department, Korea Institute of Industrial Technology (KITECH), 156, Gaetbeol-ro, Yeonsu-gu, Incheon, 21999, Republic of Korea
| | - Hyeong Min Ahn
- Digital Health Care R&D Department, Korea Institute of Industrial Technology (KITECH), 89, Yangdaegiro-gil, Ipjang-myeon, Seobuk-gu, Cheonan, 31056, Republic of Korea; Department of Biomedical Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea
| | - Chang Ki Lee
- Digital Health Care R&D Department, Korea Institute of Industrial Technology (KITECH), 89, Yangdaegiro-gil, Ipjang-myeon, Seobuk-gu, Cheonan, 31056, Republic of Korea
| | - Honggu Chun
- Department of Biomedical Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea
| | - Young Min Park
- Heat and Surface Technology R&D Department, Korea Institute of Industrial Technology (KITECH), 156, Gaetbeol-ro, Yeonsu-gu, Incheon, 21999, Republic of Korea.
| | - Kwang Bok Kim
- Digital Health Care R&D Department, Korea Institute of Industrial Technology (KITECH), 89, Yangdaegiro-gil, Ipjang-myeon, Seobuk-gu, Cheonan, 31056, Republic of Korea.
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Chen J, Ding X, Zhang D. Challenges and strategies faced in the electrochemical biosensing analysis of neurochemicals in vivo: A review. Talanta 2024; 266:124933. [PMID: 37506520 DOI: 10.1016/j.talanta.2023.124933] [Citation(s) in RCA: 9] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/15/2023] [Revised: 07/07/2023] [Accepted: 07/10/2023] [Indexed: 07/30/2023]
Abstract
Our brain is an intricate neuromodulatory network, and various neurochemicals, including neurotransmitters, neuromodulators, gases, ions, and energy metabolites, play important roles in regulating normal brain function. Abnormal release or imbalance of these substances will lead to various diseases such as Parkinson's and Alzheimer's diseases, therefore, in situ and real-time analysis of neurochemical interactions in pathophysiological conditions is beneficial to facilitate our understanding of brain function. Implantable electrochemical biosensors are capable of monitoring neurochemical signals in real time in extracellular fluid of specific brain regions because they can provide excellent temporal and spatial resolution. However, in vivo electrochemical biosensing analysis mainly faces the following challenges: First, foreign body reactions induced by microelectrode implantation, non-specific adsorption of proteins and redox products, and aggregation of glial cells, which will cause irreversible degradation of performance such as stability and sensitivity of the microsensor and eventually lead to signal loss; Second, various neurochemicals coexist in the complex brain environment, and electroactive substances with similar formal potentials interfere with each other. Therefore, it is a great challenge to design recognition molecules and tailor functional surfaces to develop in vivo electrochemical biosensors with high selectivity. Here, we take the above challenges as a starting point and detail the basic design principles for improving in vivo stability, selectivity and sensitivity of microsensors through some specific functionalized surface strategies as case studies. At the same time, we summarize surface modification strategies for in vivo electrochemical biosensing analysis of some important neurochemicals for researchers' reference. In addition, we also focus on the electrochemical detection of low basal concentrations of neurochemicals in vivo via amperometric waveform techniques, as well as the stability and biocompatibility of reference electrodes during long-term sensing, and provide an outlook on the future direction of in vivo electrochemical neurosensing.
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Affiliation(s)
- Jiatao Chen
- School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China
| | - Xiuting Ding
- School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China
| | - Dongdong Zhang
- School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China.
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15
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Singh KR, Singh P, Mallick S, Singh J, Pandey SS. Chitosan stabilized copper iodide nanoparticles enabled nano-bio-engineered platform for efficient electrochemical biosensing of dopamine. Int J Biol Macromol 2023; 253:127587. [PMID: 37866579 DOI: 10.1016/j.ijbiomac.2023.127587] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/29/2023] [Revised: 10/16/2023] [Accepted: 10/19/2023] [Indexed: 10/24/2023]
Abstract
Neurodegenerative disorders are one of the significant challenges to the aging society, as per the United Nations, where 1 in 6 people globally over 65 years of age are expected to suffer by 2050. The exact pathophysiological root of these disorders is although not known adequately, but reduced dopamine (most significant neurotransmitters) levels have been reported in people affected by Parkinson's disease. Sensitive detection and effective monitoring of dopamine can help to diagnose these neurodegenerative disorders at a very early stage, which will help to properly treat these disorders and slow down their progression. Therefore, it is crucial to detect physiological and clinically acceptable amounts of dopamine with high sensitivity and selectivity in basic pathophysiology research, medication, and illness diagnosis. Here in this present investigation, nano-bio-engineered stable chitosan stabilized copper iodide nanoparticles (CS@CuI NPs) were synthesized to engineer the active biosensing platform for developing dopamine biosensors. Initially, the as-synthesized nano-bio-engineered CS@CuI NPs were subjected to its drop-casting onto an Indium tin oxide (ITO) conducting glass substrate. This substrate platform was then utilized to immobilize tyrosinase (Tyr) enzyme by drop-casting to fabricate Tyr/CS@CuI NPs/ITO bioelectrode for the ultrasensitive determination of dopamine. Several techniques were used to characterize the structural, optical, and morphological properties of the synthesized CS@CuI NPs and Tyr/CS@CuI NPs/ITO bioelectrode. Further, the as-prepared bioelectrode was evaluated for its suitability and electrocatalytic behaviour towards dopamine by cyclic voltammetry. A perusal of the electroanalytic results of the fabricated biosensor revealed that under the optimized experimental conditions, Tyr/CS@CuI NPs/ITO bioelectrode exhibits a very high electrochemical sensitivity of 11.64 μA μM-1 cm-2 towards dopamine with the low limit of detection and quantification of 0.02 and 0.386 μM, respectively. In addition, the fabricated bioelectrode was stable up to 46 days with only 4.82 % current loss, reusable till 20 scans, and it also performed effectively while real sample analysis. Therefore, the nano-bio-engineered biosensor platform being reported can determine deficient dopamine levels in a very selective and sensitive manner, which can help adequately manage neurodegenerative disorders, further slowing down the disease progression.
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Affiliation(s)
- Kshitij Rb Singh
- Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan.
| | - Pooja Singh
- Department of Biotechnology, Faculty of Science, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh 484886, India
| | - Sadhucharan Mallick
- Department of Chemistry, Faculty of Science, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh 484886, India
| | - Jay Singh
- Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India
| | - Shyam S Pandey
- Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan.
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16
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Kouser R, Yasir Khan H, Arjmand F, Tabassum S. Synthesis and structural elucidation of a unique turn-off fluorescent sensor based on oxo-bridged tin (IV) cluster for selective detection of dopamine in biological fluids. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2023; 303:123152. [PMID: 37467591 DOI: 10.1016/j.saa.2023.123152] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/20/2023] [Revised: 06/20/2023] [Accepted: 07/12/2023] [Indexed: 07/21/2023]
Abstract
An oxo-bridged Sn (IV) Cluster, (TOC) was synthesized and fully characterized by FT-IR, UV-vis, 1H NMR, 119Sn NMR, Mass spectrometry and single crystal X-ray diffraction studies. The single-crystal X-ray analysis revealed that the crystal crystallizes in the monoclinic crystal system possessing the P 21/c space group and exhibited a distorted trigonal bipyramidal geometry. The TOC exhibited a unique turn-off fluorescence response for the selective detection of dopamine (DA) over other analytes. The stoichiometry between the TOC and DA was calculated using Job's plot. The value of the detection limit was found to be 1.33 µM. The Hirshfeld surface analysis was carried out on the crystal structure to investigate the H-H, Cl-H, Cl-Cl, Sn-Cl and Cl-C interaction studies in the molecule. Density Functional Theory (DFT) studies further supported the sensing mechanism, which closely agreed with the experimental results. Furthermore, the TOC chemosensor was used to detect DA in human blood plasma, and molecular docking studies validated the interaction between the chemosensor and protein. Confocal fluorescence imaging studies were carried out and validated TOC sensing ability for DA in human blood plasma.
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Affiliation(s)
- Robina Kouser
- Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India
| | - Huzaifa Yasir Khan
- Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India
| | - Farukh Arjmand
- Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India
| | - Sartaj Tabassum
- Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
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17
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Ramajayam K, Ganesan S, Ramesh P, Beena M, Kokulnathan T, Palaniappan A. Molecularly Imprinted Polymer-Based Biomimetic Systems for Sensing Environmental Contaminants, Biomarkers, and Bioimaging Applications. Biomimetics (Basel) 2023; 8:245. [PMID: 37366840 DOI: 10.3390/biomimetics8020245] [Citation(s) in RCA: 7] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/13/2023] [Revised: 05/20/2023] [Accepted: 06/02/2023] [Indexed: 06/28/2023] Open
Abstract
Molecularly imprinted polymers (MIPs), a biomimetic artificial receptor system inspired by the human body's antibody-antigen reactions, have gained significant attraction in the area of sensor development applications, especially in the areas of medical, pharmaceutical, food quality control, and the environment. MIPs are found to enhance the sensitivity and specificity of typical optical and electrochemical sensors severalfold with their precise binding to the analytes of choice. In this review, different polymerization chemistries, strategies used in the synthesis of MIPs, and various factors influencing the imprinting parameters to achieve high-performing MIPs are explained in depth. This review also highlights the recent developments in the field, such as MIP-based nanocomposites through nanoscale imprinting, MIP-based thin layers through surface imprinting, and other latest advancements in the sensor field. Furthermore, the role of MIPs in enhancing the sensitivity and specificity of sensors, especially optical and electrochemical sensors, is elaborated. In the later part of the review, applications of MIP-based optical and electrochemical sensors for the detection of biomarkers, enzymes, bacteria, viruses, and various emerging micropollutants like pharmaceutical drugs, pesticides, and heavy metal ions are discussed in detail. Finally, MIP's role in bioimaging applications is elucidated with a critical assessment of the future research directions for MIP-based biomimetic systems.
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Affiliation(s)
- Kalaipriya Ramajayam
- Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
- Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
| | - Selvaganapathy Ganesan
- Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
- Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
| | - Purnimajayasree Ramesh
- Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
- School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
| | - Maya Beena
- Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
- School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
| | - Thangavelu Kokulnathan
- Department of Electro-Optical Engineering, National Taipei University of Technology, Taipei 106, Taiwan
| | - Arunkumar Palaniappan
- Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India
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18
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Musa AM, Kiely J, Luxton R, Honeychurch KC. An Electrochemical Screen-Printed Sensor Based on Gold-Nanoparticle-Decorated Reduced Graphene Oxide-Carbon Nanotubes Composites for the Determination of 17-β Estradiol. BIOSENSORS 2023; 13:bios13040491. [PMID: 37185565 PMCID: PMC10136424 DOI: 10.3390/bios13040491] [Citation(s) in RCA: 13] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/14/2023] [Revised: 04/07/2023] [Accepted: 04/10/2023] [Indexed: 05/17/2023]
Abstract
In this study, a screen-printed electrode (SPE) modified with gold-nanoparticle-decorated reduced graphene oxide-carbon nanotubes (rGO-AuNPs/CNT/SPE) was used for the determination of estradiol (E2). The AuNPs were produced through an eco-friendly method utilising plant extract, eliminating the need for severe chemicals, and remove the requirements of sophisticated fabrication methods and tedious procedures. In addition, rGO-AuNP serves as a dispersant for the CNT to improve the dispersion stability of CNTs. The composite material, rGO-AuNPs/CNT, underwent characterisation through scanning electron microscopy (SEM), ultraviolet-visible absorption spectroscopy (UV-vis), Fourier-transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). The electrochemical performance of the modified SPE for estradiol oxidation was characterised using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The rGO-AuNPs/CNT/SPE exhibited a notable improvement compared to bare/SPE and GO-CNT/SPE, as evidenced by the relative peak currents. Additionally, we employed a baseline correction algorithm to accurately adjust the sensor response while eliminating extraneous background components that are typically present in voltammetric experiments. The optimised estradiol sensor offers linear sensitivity from 0.05-1.00 µM, with a detection limit of 3 nM based on three times the standard deviation (3δ). Notably, this sensing approach yields stable, repeatable, and reproducible outcomes. Assessment of drinking water samples indicated an average recovery rate of 97.5% for samples enriched with E2 at concentrations as low as 0.5 µM%, accompanied by only a modest coefficient of variation (%CV) value of 2.7%.
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Affiliation(s)
- Auwal M Musa
- Institute of Bio-Sensing Technology (IBST), University of the West of England, Bristol BS16 1QY, UK
| | - Janice Kiely
- Centre for Research in Biosciences (CRIB), School of Applied Sciences, University of the West of England, Bristol BS16 1QY, UK
| | - Richard Luxton
- Centre for Research in Biosciences (CRIB), School of Applied Sciences, University of the West of England, Bristol BS16 1QY, UK
| | - Kevin C Honeychurch
- Institute of Bio-Sensing Technology (IBST), University of the West of England, Bristol BS16 1QY, UK
- Centre for Research in Biosciences (CRIB), School of Applied Sciences, University of the West of England, Bristol BS16 1QY, UK
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19
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Park JH, Eom YS, Kim TH. Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters. BIOSENSORS 2023; 13:bios13040413. [PMID: 37185488 PMCID: PMC10136356 DOI: 10.3390/bios13040413] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/23/2023] [Revised: 03/20/2023] [Accepted: 03/21/2023] [Indexed: 05/17/2023]
Abstract
In recent years, there has been an increased demand for highly sensitive and selective biosensors for neurotransmitters, owing to advancements in science and technology. Real-time sensing is crucial for effective prevention of neurological and cardiovascular diseases. In this review, we summarise the latest progress in aptamer-based biosensor technology, which offers the aforementioned advantages. Our focus is on various biomaterials utilised to ensure the optimal performance and high selectivity of aptamer-based biosensors. Overall, this review aims to further aptamer-based biosensor technology.
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Affiliation(s)
- Joon-Ha Park
- School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
| | - Yun-Sik Eom
- School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
| | - Tae-Hyung Kim
- School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
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20
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Wang H, Feng Z, Lin F, Zhao Y, Hu Y, Yang Q, Zou Y, Zhao Y, Yang R. Research on Temperature-Switched Dopamine Electrochemical Sensor Based on Thermosensitive Polymers and MWCNTs. Polymers (Basel) 2023; 15:polym15061465. [PMID: 36987245 PMCID: PMC10058576 DOI: 10.3390/polym15061465] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2023] [Revised: 03/07/2023] [Accepted: 03/08/2023] [Indexed: 03/18/2023] Open
Abstract
A temperature-controlled electrochemical sensor was constructed based on a composite membrane composed of temperature-sensitive polymer poly (N-isopropylacrylamide) (PNIPAM) and carboxylated multi-walled carbon nanotubes (MWCNTs-COOH). The sensor has good temperature sensitivity and reversibility in detecting Dopamine (DA). At low temperatures, the polymer is stretched to bury the electrically active sites of carbon nanocomposites. Dopamine cannot exchange electrons through the polymer, representing an “OFF” state. On the contrary, in a high-temperature environment, the polymer shrinks to expose electrically active sites and increases the background current. Dopamine can normally carry out redox reactions and generate response currents, indicating the “ON” state. In addition, the sensor has a wide detection range (from 0.5 μM to 150 μM) and low LOD (193 nM). This switch-type sensor provides new avenues for the application of thermosensitive polymers.
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Affiliation(s)
- Haixiu Wang
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Zufei Feng
- School of Science, Xi’an University of Technology, Xi’an 710048, China
- Correspondence:
| | - Fupeng Lin
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Yan Zhao
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Yangfan Hu
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Qian Yang
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Yiming Zou
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Yingjuan Zhao
- School of Science, Xi’an University of Technology, Xi’an 710048, China
| | - Rong Yang
- International Research Center for Composite and Intelligent Manufacturing Technology, Institute of Chemical Power Sources, Materials and Engineering College, Xi’an University of Technology, Xi’an 710048, China
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21
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Li J, Reimers A, Dang KM, Brunk MGK, Drewes J, Hirsch UM, Willems C, Schmelzer CEH, Groth T, Nia AS, Feng X, Adelung R, Sacher WD, Schütt F, Poon JKS. 3D printed neural tissues with in situ optical dopamine sensors. Biosens Bioelectron 2023; 222:114942. [PMID: 36493722 DOI: 10.1016/j.bios.2022.114942] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2022] [Revised: 10/15/2022] [Accepted: 11/21/2022] [Indexed: 11/28/2022]
Abstract
Engineered neural tissues serve as models for studying neurological conditions and drug screening. Besides observing the cellular physiological properties, in situ monitoring of neurochemical concentrations with cellular spatial resolution in such neural tissues can provide additional valuable insights in models of disease and drug efficacy. In this work, we demonstrate the first three-dimensional (3D) tissue cultures with embedded optical dopamine (DA) sensors. We developed an alginate/Pluronic F127 based bio-ink for human dopaminergic brain tissue printing with tetrapodal-shaped-ZnO microparticles (t-ZnO) additive as the DA sensor. DA quenches the autofluorescence of t-ZnO in physiological environments, and the reduction of the fluorescence intensity serves as an indicator of the DA concentration. The neurons that were 3D printed with the t-ZnO showed good viability, and extensive 3D neural networks were formed within one week after printing. The t-ZnO could sense DA in the 3D printed neural network with a detection limit of 0.137 μM. The results are a first step toward integrating tissue engineering with intensiometric biosensing for advanced artificial tissue/organ monitoring.
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Affiliation(s)
- Jianfeng Li
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Canada.
| | - Armin Reimers
- Institute for Materials Science, Kiel University, 24143, Kiel, Germany
| | - Ka My Dang
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Canada
| | - Michael G K Brunk
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Canada
| | - Jonas Drewes
- Institute for Materials Science, Kiel University, 24143, Kiel, Germany
| | - Ulrike M Hirsch
- Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Straße 1, 06120, Halle, Germany
| | - Christian Willems
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany
| | - Christian E H Schmelzer
- Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Straße 1, 06120, Halle, Germany
| | - Thomas Groth
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany
| | - Ali Shaygan Nia
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Faculty of Chemistry and Food Chemistry & Center for Advancing Electronics Dresden, Technische Universität Dresden, Dresden, 01062, Germany
| | - Xinliang Feng
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Faculty of Chemistry and Food Chemistry & Center for Advancing Electronics Dresden, Technische Universität Dresden, Dresden, 01062, Germany
| | - Rainer Adelung
- Institute for Materials Science, Kiel University, 24143, Kiel, Germany; Kiel Nano, Surface and Interface Science KiNSIS, Kiel University, Christian-Albrechts-Platz 4, D-24118 Kiel, Germany
| | - Wesley D Sacher
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Canada
| | - Fabian Schütt
- Institute for Materials Science, Kiel University, 24143, Kiel, Germany; Kiel Nano, Surface and Interface Science KiNSIS, Kiel University, Christian-Albrechts-Platz 4, D-24118 Kiel, Germany
| | - Joyce K S Poon
- Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Canada; Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, Canada.
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22
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Ranasinghe JC, Wang Z, Huang S. Raman Spectroscopy on Brain Disorders: Transition from Fundamental Research to Clinical Applications. BIOSENSORS 2022; 13:27. [PMID: 36671862 PMCID: PMC9855372 DOI: 10.3390/bios13010027] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 11/18/2022] [Revised: 12/13/2022] [Accepted: 12/23/2022] [Indexed: 06/17/2023]
Abstract
Brain disorders such as brain tumors and neurodegenerative diseases (NDs) are accompanied by chemical alterations in the tissues. Early diagnosis of these diseases will provide key benefits for patients and opportunities for preventive treatments. To detect these sophisticated diseases, various imaging modalities have been developed such as computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET). However, they provide inadequate molecule-specific information. In comparison, Raman spectroscopy (RS) is an analytical tool that provides rich information about molecular fingerprints. It is also inexpensive and rapid compared to CT, MRI, and PET. While intrinsic RS suffers from low yield, in recent years, through the adoption of Raman enhancement technologies and advanced data analysis approaches, RS has undergone significant advancements in its ability to probe biological tissues, including the brain. This review discusses recent clinical and biomedical applications of RS and related techniques applicable to brain tumors and NDs.
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Affiliation(s)
| | | | - Shengxi Huang
- Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USA
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23
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Zheng X, Liu J, Li M, Hua Y, Liang X, Zhang S, Zhang X, Shao Y. Dual-Nanopipettes for the Detection of Single Nanoparticles and Small Molecules. Anal Chem 2022; 94:17431-17438. [PMID: 36495265 DOI: 10.1021/acs.analchem.2c03344] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Abstract
Nanopore sensing is blooming due to its label-free and high sensitivity features. As a novel nanopore, a droplet is formed at the orifice of a dual-nanopipette, which allows for the translocation of analytes through the two channels at a relatively low speed and the promotion of signal-to-noise ratio. However, nanopore sensing based on the principle of current blockage requires the pore size to be comparable to that of the single entity, which poses a huge challenge for the direct detection of small molecules. In this work, gold nanoparticles (Au NPs) modified with sulfhydryl poly(ethylene glycol) (PEG-SH) or aptamers were detected successfully. The size difference of Au NPs and the interaction between Au NPs and dual-nanopipettes could be distinguished sensitively. Furthermore, Au NPs modified with designed aptamers will produce different blocking current after capturing the corresponding small molecules (e.g., dopamine and serotonin). Even non-electroactive ions, such as potassium ions, can also be detected, which is difficult to sense based on redox reactions, and further illustrates that the change of surface properties of nanoparticles is responsible for the detection. This work expands the application of nanopipette sensing for Au NPs and provides a universal platform for the small-molecule detection, which has the potential application in biosensing.
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Affiliation(s)
- Xinhe Zheng
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Junjie Liu
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Mingzhi Li
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Yutong Hua
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Xu Liang
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Shudong Zhang
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Xianhao Zhang
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
| | - Yuanhua Shao
- Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
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Vaneev AN, Timoshenko RV, Gorelkin PV, Klyachko NL, Korchev YE, Erofeev AS. Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives. NANOMATERIALS (BASEL, SWITZERLAND) 2022; 12:3736. [PMID: 36364512 PMCID: PMC9656311 DOI: 10.3390/nano12213736] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/23/2022] [Revised: 10/16/2022] [Accepted: 10/21/2022] [Indexed: 05/14/2023]
Abstract
Electrochemical nano- and microsensors have been a useful tool for measuring different analytes because of their small size, sensitivity, and favorable electrochemical properties. Using such sensors, it is possible to study physiological mechanisms at the cellular, tissue, and organ levels and determine the state of health and diseases. In this review, we highlight recent advances in the application of electrochemical sensors for measuring neurotransmitters, oxygen, ascorbate, drugs, pH values, and other analytes in vivo. The evolution of electrochemical sensors is discussed, with a particular focus on the development of significant fabrication schemes. Finally, we highlight the extensive applications of electrochemical sensors in medicine and biological science.
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Affiliation(s)
- Alexander N. Vaneev
- Research Laboratory of Biophysics, National University of Science and Technology “MISiS”, 119049 Moscow, Russia
- Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia
| | - Roman V. Timoshenko
- Research Laboratory of Biophysics, National University of Science and Technology “MISiS”, 119049 Moscow, Russia
| | - Petr V. Gorelkin
- Research Laboratory of Biophysics, National University of Science and Technology “MISiS”, 119049 Moscow, Russia
| | - Natalia L. Klyachko
- Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia
| | - Yuri E. Korchev
- Department of Medicine, Imperial College London, London W12 0NN, UK
| | - Alexander S. Erofeev
- Research Laboratory of Biophysics, National University of Science and Technology “MISiS”, 119049 Moscow, Russia
- Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia
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25
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Halicka K, Meloni F, Czok M, Spychalska K, Baluta S, Malecha K, Pilo MI, Cabaj J. New Trends in Fluorescent Nanomaterials-Based Bio/Chemical Sensors for Neurohormones Detection-A Review. ACS OMEGA 2022; 7:33749-33768. [PMID: 36188279 PMCID: PMC9520559 DOI: 10.1021/acsomega.2c04134] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 07/01/2022] [Accepted: 09/05/2022] [Indexed: 06/16/2023]
Abstract
The study of neurotransmitters and stress hormones allows the determination of indicators of the current stress load in the body. These species also create a proper strategy of stress protection. Nowadays, stress is a general factor that affects the population, and it may cause a wide range of serious disorders. Abnormalities in the level of neurohormones, caused by chronic psychological stress, can occur in, for instance, corporate employees, health care workers, shift workers, policemen, or firefighters. Here we present a new nanomaterials-based sensors technology development for the determination of neurohormones. We focus on fluorescent sensors/biosensors that utilize nanomaterials, such as quantum dots or carbon nanomaterials. Nanomaterials, owing to their diversity in size and shape, have been attracting increasing attention in sensing or bioimaging. They possess unique properties, such as fluorescent, electronic, or photoluminescent features. In this Review, we summarize new trends in adopting nanomaterials for applications in fluorescent sensors for neurohormone monitoring.
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Affiliation(s)
- Kinga Halicka
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
| | - Francesca Meloni
- Department
of Chemistry and Pharmacy, University of
Sassari, Via Vienna 2, 07100 Sassari, Italy
| | - Mateusz Czok
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
| | - Kamila Spychalska
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
| | - Sylwia Baluta
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
| | - Karol Malecha
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
| | - Maria I. Pilo
- Department
of Chemistry and Pharmacy, University of
Sassari, Via Vienna 2, 07100 Sassari, Italy
| | - Joanna Cabaj
- Faculty
of Chemistry and Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
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26
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Sensor Technology and Intelligent Systems in Anorexia Nervosa: Providing Smarter Healthcare Delivery Systems. BIOMED RESEARCH INTERNATIONAL 2022; 2022:1955056. [PMID: 36193321 PMCID: PMC9526573 DOI: 10.1155/2022/1955056] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/20/2022] [Accepted: 09/06/2022] [Indexed: 11/22/2022]
Abstract
Ubiquitous technology, big data, more efficient electronic health records, and predictive analytics are now at the core of smart healthcare systems supported by artificial intelligence. In the present narrative review, we focus on sensing technologies for the healthcare of Anorexia Nervosa (AN). We employed a framework inspired by the Interpersonal Neurobiology Theory (IPNB), which posits that human experience is characterized by a flow of energy and information both within us (within our whole body), and between us (in the connections we have with others and with nature). In line with this framework, we focused on sensors designed to evaluate bodily processes (body sensors such as implantable sensors, epidermal sensors, and wearable and portable sensors), human social interaction (sociometric sensors), and the physical environment (indoor and outdoor ambient sensors). There is a myriad of man-made sensors as well as nature-based sensors such as plants that can be used to design and deploy intelligent systems for human monitoring and healthcare. In conclusion, sensing technologies and intelligent systems can be employed for smarter healthcare of AN and help to relieve the burden of health professionals. However, there are technical, ethical, and environmental sustainability issues that must be considered prior to implementing these systems. A joint collaboration of professionals and other members of the society involved in the healthcare of individuals with AN can help in the development of these systems. The evolution of cyberphysical systems should also be considered in these collaborations.
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Revanappa SK, Soni I, Siddalinganahalli M, Jayaprakash GK, Flores-Moreno R, Bananakere Nanjegowda C. A Fukui Analysis of an Arginine-Modified Carbon Surface for the Electrochemical Sensing of Dopamine. MATERIALS (BASEL, SWITZERLAND) 2022; 15:6337. [PMID: 36143660 PMCID: PMC9506051 DOI: 10.3390/ma15186337] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 08/17/2022] [Revised: 09/05/2022] [Accepted: 09/08/2022] [Indexed: 06/16/2023]
Abstract
Amino acid-modified carbon interfaces have huge applications in developing electrochemical sensing applications. Earlier reports suggested that the amine group of amino acids acted as an oxidation center at the amino acid-modified electrode interface. It was interesting to locate the oxidation centers of amino acids in the presence of guanidine. In the present work, we modeled the arginine-modified carbon interface and utilized frontier molecular orbitals and analytical Fukui functions based on the first principle study computations to analyze arginine-modified CPE (AMCPE) at a molecular level. The frontier molecular orbital and analytical Fukui results suggest that the guanidine (oxidation) and carboxylic acid (reduction) groups of arginine act as additional electron transfer sites on the AMCPE surface. To support the theoretical observations, we prepared the arginine-modified CPE (AMCPE) for the cyclic voltammetric sensing of dopamine (DA). The AMCPE showed excellent performance in detecting DA in blood serum samples.
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Affiliation(s)
- Santhosh Kumar Revanappa
- Department of Chemistry, University B.D.T. College of Engineering Visvesvaraya Technological University, Davangere 577004, India
| | - Isha Soni
- Laboratory of Quantum Electrochemistry, School of Advacned Chemical Sciences, Shoolini University, Solan 173229, India
| | - Manjappa Siddalinganahalli
- Department of Chemistry, University B.D.T. College of Engineering Visvesvaraya Technological University, Davangere 577004, India
| | - Gururaj Kudur Jayaprakash
- Laboratory of Quantum Electrochemistry, School of Advacned Chemical Sciences, Shoolini University, Solan 173229, India
- Department of Chemistry, Nitte Meenakshi Institute of Technology, Bangalore 560064, India
| | - Roberto Flores-Moreno
- Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad Guadalajara, Blvd. Marcelino García Barragán 1421, Guadalajara C.P. 44430, Mexico
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Carvalho da Silva VN, Farias EADO, Araújo AR, Xavier Magalhães FE, Neves Fernandes JR, Teles Souza JM, Eiras C, Alves da Silva D, Hugo do Vale Bastos V, Teixeira SS. Rapid and selective detection of dopamine in human serum using an electrochemical sensor based on zinc oxide nanoparticles, nickel phthalocyanines, and carbon nanotubes. Biosens Bioelectron 2022; 210:114211. [PMID: 35468419 DOI: 10.1016/j.bios.2022.114211] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/04/2022] [Revised: 03/17/2022] [Accepted: 03/20/2022] [Indexed: 12/29/2022]
Abstract
Composite materials have gained significant attention owing to the synergistic effects of their constituent materials, thereby facilitating their utilization in new applications or in improving the existing ones. In this study, a composite based on nickel phthalocyanine (NiTsPc), zinc oxide nanoparticles (ZnONPs), and carbon nanotubes (CNT) was developed and subsequently immobilized on a pyrolytic graphite electrode (PGE). The PGE/NiTsPc-ZnONPs-CNT was identified as a selective catalytic hybrid system for detection of neurotransmitter dopamine (DA). The electrochemical and morphological characterizations were conducted using atomic force microscopy (AFM). Chronoamperometry and differential pulse voltammetry (DPV) were used to detect DA and detection limits of 24 nM and 7.0 nM was found, respectively. In addition, the effects of some possible DA interferents, such as ascorbic acid, uric acid, and serotonin, on DA response were evaluated. Their presence did not show significant variations in the DA electrochemical response. The high specificity and sensitivity of PGE/NiTsPc-ZnONPs-CNT for DA enabled its direct detection in human serum without sample pretreatment as well as in DA-enriched serum samples, whose recovery levels were close to 100%, thereby confirming the effectiveness of the proposed method. In general, PGE/NiTsPc-ZnONPs-CNT is a promising candidate for future applications in clinical diagnosis.
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Affiliation(s)
- Valécia Natália Carvalho da Silva
- Laboratório de Neuroinovação Tecnológica & Mapeamento Cerebral - NITLAB, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil.
| | - Emanuel Airton de O Farias
- Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil.
| | - Alyne R Araújo
- Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Francisco Elezier Xavier Magalhães
- Laboratório de Neuroinovação Tecnológica & Mapeamento Cerebral - NITLAB, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Jacks Renan Neves Fernandes
- Laboratório de Neuroinovação Tecnológica & Mapeamento Cerebral - NITLAB, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Jéssica Maria Teles Souza
- Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Carla Eiras
- Laboratório de Pesquisa e Desenvolvimento de Novos Materiais e Sistemas Sensores - MATSENS, Centro de Tecnologia, Universidade Federal do Piauí, Teresina, PI 64049-550, Brazil.
| | - Durcilene Alves da Silva
- Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Victor Hugo do Vale Bastos
- Laboratório de Mapeamento e Funcionalidade Cerebral - LAMCEF, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
| | - Silmar Silva Teixeira
- Laboratório de Neuroinovação Tecnológica & Mapeamento Cerebral - NITLAB, Universidade Federal do Delta do Parnaíba, Parnaíba, PI 64202-020, Brazil
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29
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Portable, Disposable, Biomimetic Electrochemical Sensors for Analyte Detection in a Single Drop of Whole Blood. CHEMOSENSORS 2022. [DOI: 10.3390/chemosensors10070263] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/13/2023]
Abstract
Current diagnostics call for rapid, sensitive, and selective screening of physiologically important biomarkers. Point-of-care (POC) devices for the rapid, reliable, and easy acquisition of bioinformation at, or near the patient, offer opportunities for better healthcare management. Electrochemical biosensors with high sensitivity and ease of miniaturization are advantageous for such applications. We report a photolithographically micropatterned PEDOT:PSS and silk protein-based fully organic 3-electrode sensor (O3ES) for ultralow volume (single drop—10 µL) detection of analytes in whole blood. The O3ES produces reliable electrochemical signals in whole blood from a mouse model with minimal biofouling interference. The O3ES is demonstrated as a portable device for the simultaneous detection of dopamine, ascorbic acid and uric acid using voltammetry techniques. The O3ES displays excellent sensitivity towards each analyte in whole blood, and in the presence of each other. The water-based, ambient processing of the sensors allows the immobilization of enzymes in the organic working electrode. Amperometric detection of uric acid via uricase with high sensitivity in whole blood is demonstrated. Finally, the performance of the O3ES under enzymatic degradation is studied by monitoring sensitivity over an operating lifetime of ~14 days. This work demonstrates the realization of low-cost, disposable POC sensors capable of detecting blood metabolites using ultralow sample volumes.
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30
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Review—Recent Progress in Graphene Based Modified Electrodes for Electrochemical Detection of Dopamine. CHEMOSENSORS 2022. [DOI: 10.3390/chemosensors10070249] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/25/2022]
Abstract
Graphene and its derivatives have been widely used for the electrochemical detection of dopamine (DA) neurotransmitter, thanks to its high surface area and excellent conductivity. Modified graphene and graphene-based nanocomposites have shown improved catalytic activity towards DA detection. Various modification approaches have been taken, including heteroatom doping and association with other nanomaterials. This review summarizes and highlights the recent advances in graphene-based electrodes for the electrochemical detection of DA. It also aims to provide an overview of the advantages of using polymer as a linker platform to form graphene-based nanocomposites applied to electrochemical DA sensors.
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Direct and Sensitive Detection of Dopamine Using Carbon Quantum Dots Based Refractive Index Surface Plasmon Resonance Sensor. NANOMATERIALS 2022; 12:nano12111799. [PMID: 35683655 PMCID: PMC9182140 DOI: 10.3390/nano12111799] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/06/2022] [Revised: 05/20/2022] [Accepted: 05/20/2022] [Indexed: 02/07/2023]
Abstract
Abnormality of dopamine (DA), a vital neurotransmitter in the brain’s neuronal pathways, causes several neurological diseases. Rapid and sensitive sensors for DA detection are required for early diagnosis of such disorders. Herein, a carbon quantum dot (CQD)-based refractive index surface plasmon resonance (SPR) sensor was designed. The sensor performance was evaluated for various concentrations of DA. Increasing DA levels yielded blue-shifted SPR dips. The experimental findings revealed an excellent sensitivity response of 0.138°/pM in a linear range from 0.001 to 100 pM and a high binding affinity of 6.234 TM−1. The effects of varied concentrations of DA on the optical characteristics of CQD thin film were further proved theoretically. Increased DA levels decreased the thickness and real part of the refractive index of CQD film, according to fitting results. Furthermore, the observed reduction in surface roughness using AFM demonstrated that DA was bound to the sensor layer. This, in turn, explained the blue shift in SPR reflectance curves. This optical sensor offers great potential as a trustworthy solution for direct measurement due to its simple construction, high sensitivity, and other sensing features.
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32
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Wang Y, Wang D, Dong S, Qiao J, Zeng Z, Shao S. A visible-light-driven photoelectrochemical sensing platform based on the BiVO4/FeOOH photoanode for dopamine detection. Electrochim Acta 2022. [DOI: 10.1016/j.electacta.2022.140207] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/03/2022]
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Evaluation of Structural and Optical Properties of Graphene Oxide-Polyvinyl Alcohol Thin Film and Its Potential for Pesticide Detection Using an Optical Method. PHOTONICS 2022. [DOI: 10.3390/photonics9050300] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/04/2023]
Abstract
In the present work, graphene oxide (GO)–polyvinyl alcohol (PVA) composites thin film has been successfully synthesized and prepared by spin coating techniques. Then, the properties and morphology of the samples were characterized using Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), and atomic force microscopy (AFM). Experimental FTIR results for GO–PVA thin film demonstrated the existence of important functional groups such as -CH2 stretching, C=O stretching, and O–H stretching. Furthermore, UV-Vis analysis indicated that the GO–PVA thin film had the highest absorbance that can be observed at wavelengths ranging from 200 to 500 nm with a band gap of 4.082 eV. The surface morphology of the GO–PVA thin film indicated the thickness increased when in contact with carbaryl. The incorporation of the GO–PVA thin film with an optical method based on the surface plasmon resonance (SPR) phenomenon demonstrated a positive response for the detection of carbaryl pesticide as low as 0.02 ppb. This study has successfully proposed that the GO–PVA thin film has high potential as a polymer nanomaterial-based SPR sensor for pesticide detection.
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34
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Tortolini C, Cass AEG, Pofi R, Lenzi A, Antiochia R. Microneedle-based nanoporous gold electrochemical sensor for real-time catecholamine detection. Mikrochim Acta 2022; 189:180. [PMID: 35391571 PMCID: PMC8989844 DOI: 10.1007/s00604-022-05260-2] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2021] [Accepted: 03/04/2022] [Indexed: 11/21/2022]
Abstract
Dopamine (DA), epinephrine (EP), and norepinephrine (NEP) are the main catecholamine of clinical interest, as they play crucial roles in the regulation of nervous and cardiovascular systems and are involved in some brain behaviors, such as stress, panic, anxiety, and depression. Therefore, there is an urgent need for a reliable sensing device able to provide their continuous monitoring in a minimally invasive manner. In this work, the first highly nanoporous gold (h-nPG) microneedle-based sensor is presented for continuous monitoring of catecholamine in interstitial fluid (ISF). The h-nPG microneedle-based gold electrode was prepared by a simple electrochemical self-templating method that involves two steps, gold electrodeposition and hydrogen bubbling at the electrode surface, realized by sweeping the potential between + 0.8 V and 0 V vs Ag/AgCl for 25 scans in a 10 mM HAuCl4 solution containing 2.5 M NH4Cl, and successively applying a fixed potential of − 2 V vs Ag/AgCl for 60 s. The resulting microneedle-based h-nPG sensor displays an interference-free total catecholamine detection expressed as NEP concentration, with a very low LOD of 100 nM, excellent sensitivity and stability, and fast response time (< 4 s). The performance of the h-nPG microneedle array sensor was successively assessed in artificial ISF and in a hydrogel skin model at typical physiological concentrations.
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Affiliation(s)
- Cristina Tortolini
- Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy
| | - Anthony E G Cass
- Department of Chemistry & Institute of Biomedical Engineering, Imperial College, London, UK
| | - Riccardo Pofi
- Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
| | - Andrea Lenzi
- Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
| | - Riccarda Antiochia
- Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
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Kamal Eddin FB, Fen YW, Omar NAS, Liew JYC, Daniyal WMEMM. Femtomolar detection of dopamine using surface plasmon resonance sensor based on chitosan/graphene quantum dots thin film. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2021; 263:120202. [PMID: 34333400 DOI: 10.1016/j.saa.2021.120202] [Citation(s) in RCA: 13] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/13/2021] [Revised: 06/23/2021] [Accepted: 07/15/2021] [Indexed: 06/13/2023]
Abstract
Due to the crucial role of dopamine (DA) in health and peripheral nervous systems, it is particularly important to develop an efficient and accurate sensor to monitor and determine DA concentrations for diagnostic purposes and diseases prevention. Up to now, using surface plasmon resonance (SPR) sensors in DA determination is very limited and its application still at the primary stage. In this work, a simple and ultra-sensitive SPR sensor was constructed for DA detection by preparation of chitosan- graphene quantum dots (CS-GQDs) thin film as the sensing layer. Other SPR measurements were conducted using different sensing layers; GQDs, CS for comparison. The proposed thin films were prepared by spin coating technique. The developed CS-GQDs thin film-based SPR sensor was successfully tested in DA concentration range from 0 fM to 1 pM. The designed SPR sensor showed outstanding performance in detecting DA sensitively (S = 0.011°/fM, R2 = 0.8174) with low detection limit of 1.0 fM has been achieved for the first time. The increased angular shift of SPR dip, narrow full width half maximum of the SPR curves, excellent signal-to-noise ratio and figure of merit, and a binding affinity constant (KA) of 2.962 PM-1 demonstrated the potential of this sensor to detect DA with high accuracy. Overall, it was concluded that the proposed sensor would serve as a valuable tool in clinical diagnostic for the serious neurological disorders. This in turns has a significant socio-economic impact.
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Affiliation(s)
- Faten Bashar Kamal Eddin
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
| | - Yap Wing Fen
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
| | - Nur Alia Sheh Omar
- Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
| | - Josephine Ying Chyi Liew
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
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Yang Y, Weber S. A Comprehensive Examination of Voltammetric Parameters in Fourier Transform Sine‐Wave Voltammetry (FT‐SWV) Leading to Concentration Linearity and High Signal‐to‐Background Ratio. ELECTROANAL 2021. [DOI: 10.1002/elan.202100403] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
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37
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Patella B, Sortino A, Mazzara F, Aiello G, Drago G, Torino C, Vilasi A, O'Riordan A, Inguanta R. Electrochemical detection of dopamine with negligible interference from ascorbic and uric acid by means of reduced graphene oxide and metals-NPs based electrodes. Anal Chim Acta 2021; 1187:339124. [PMID: 34753568 DOI: 10.1016/j.aca.2021.339124] [Citation(s) in RCA: 22] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/16/2021] [Revised: 08/31/2021] [Accepted: 09/28/2021] [Indexed: 01/22/2023]
Abstract
Dopamine is an important neurotransmitter involved in many human biological processes as well as in different neurodegenerative diseases. Monitoring the concentration of dopamine in biological fluids, i.e., blood and urine is an effective way of accelerating the early diagnosis of these types of diseases. Electrochemical sensors are an ideal choice for real-time screening of dopamine as they can achieve fast, portable inexpensive and accurate measurements. In this work, we present electrochemical dopamine sensors based on reduced graphene oxide coupled with Au or Pt nanoparticles. Sensors were developed by co-electrodeposition onto a flexible substrate, and a systematic investigation concerning the electrodeposition parameters (concentration of precursors, deposition time and potential) was carried out to maximize the sensitivity of the dopamine detection. Square wave voltammetry was used as an electrochemical technique that ensured a high sensitive detection in the nM range. The sensors were challenged against synthetic urine in order to simulate a real sample detection scenario where dopamine concentrations are usually lower than 600 nM. Our sensors show a negligible interference from uric and ascorbic acids which did not affect sensor performance. A wide linear range (0.1-20 μm for gold nanoparticles, 0.1-10 μm for platinum nanoparticles) with high sensitivity (6.02 and 7.19 μA μM-1 cm-2 for gold and platinum, respectively) and a low limit of detection (75 and 62 nM for Au and Pt, respectively) were achieved. Real urine samples were also assayed, where the concentrations of dopamine detected aligned very closely to measurements undertaken using conventional laboratory techniques. Sensor fabrication employed a cost-effective production process with the possibility of also being integrated into flexible substrates, thus allowing for the possible development of wearable sensing devices.
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Affiliation(s)
- Bernardo Patella
- Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy
| | - Alessia Sortino
- Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy
| | - Francesca Mazzara
- Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy
| | - Giuseppe Aiello
- Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy
| | - Giuseppe Drago
- Dipartimento di Ingegneria, Università Degli Studi di Palermo, Italy
| | - Claudia Torino
- Istituto di Fisiologia Clinica (IFC)-Consiglio Nazionale Delle Ricerche-Reggio Calabria-Italy, Italy
| | - Antonio Vilasi
- Istituto di Fisiologia Clinica (IFC)-Consiglio Nazionale Delle Ricerche-Reggio Calabria-Italy, Italy
| | - Alan O'Riordan
- Nanotechnology Group, Tyndall National Institute, University College Cork, Dyke Prade, Cork, Ireland
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Baluchová S, Brycht M, Taylor A, Mortet V, Krůšek J, Dittert I, Sedláková S, Klimša L, Kopeček J, Schwarzová-Pecková K. Enhancing electroanalytical performance of porous boron-doped diamond electrodes by increasing thickness for dopamine detection. Anal Chim Acta 2021; 1182:338949. [PMID: 34602205 DOI: 10.1016/j.aca.2021.338949] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/06/2021] [Revised: 07/20/2021] [Accepted: 08/11/2021] [Indexed: 12/24/2022]
Abstract
Novel porous boron-doped diamond (BDDporous)-based materials have attracted lots of research interest due to their enhanced detection ability and biocompatibility, favouring them for use in neuroscience. This study reports on morphological, spectral, and electrochemical characterisation of three BDDporous electrodes of different thickness given by a number of deposited layers (2, 3 and 5). These were prepared using microwave plasma-enhanced chemical vapour deposition on SiO2 nanofiber-based scaffolds. Further, the effect of number of layers and poly-l-lysine coating, commonly employed in neuron cultivation experiments, on sensing properties of the neurotransmitter dopamine in a pH 7.4 phosphate buffer media was investigated. The boron doping level of ∼2 × 1021 atoms cm-3 and increased content of non-diamond (sp2) carbon in electrodes with more layers was evaluated by Raman spectroscopy. Cyclic voltammetric experiments revealed reduced working potential windows (from 2.4 V to 2.2 V), higher double-layer capacitance values (from 405 μF cm-2 to 1060 μF cm-2), enhanced rates of electron transfer kinetics and larger effective surface areas (from 5.04 mm2 to 7.72 mm2), when the number of porous layers increases. For dopamine, a significant boost in analytical performance was recognized with increasing number of layers using square-wave voltammetry: the highest sensitivity of 574.1 μA μmol-1 L was achieved on a BDDporous electrode with five layers and dropped to 35.9 μA μmol-1 L when the number of layers decreased to two. Consequently, the lowest detection limit of 0.20 μmol L-1 was obtained on a BDDporous electrode with five layers. Moreover, on porous electrodes, enhanced selectivity for dopamine detection in the presence of ascorbic acid and uric acid was demonstrated. The application of poly-l-lysine coating on porous electrode surface resulted in a decrease in dopamine peak currents by 17% and 60% for modification times of 1 h and 15 h, respectively. Hence, both examined parameters, the number of deposited porous layers and the presence of poly-l-lysine coating, were proved to considerably affect the characteristics and performance of BDDporous electrodes.
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Affiliation(s)
- Simona Baluchová
- Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 128 00, Prague 2, Czech Republic; FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic
| | - Mariola Brycht
- University of Lodz, Faculty of Chemistry, Department of Inorganic and Analytical Chemistry, Tamka 12, 91-403, Łódź, Poland
| | - Andrew Taylor
- FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic
| | - Vincent Mortet
- FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic; Czech Technical University in Prague, Faculty of Biomedical Engineering, Sítná Sq. 3105, 272 01, Kladno, Czech Republic
| | - Jan Krůšek
- Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czech Republic
| | - Ivan Dittert
- Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czech Republic
| | - Silvia Sedláková
- FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic
| | - Ladislav Klimša
- FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic
| | - Jaromír Kopeček
- FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, 182 21, Prague 8, Czech Republic
| | - Karolina Schwarzová-Pecková
- Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 128 00, Prague 2, Czech Republic.
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Effendy MA, Yunusa S, Zain ZM, Hassan Z. Real time monitoring of dopamine release evoked by mitragynine (Kratom): An insight through electrochemical sensor. Neurosci Lett 2021; 763:136183. [PMID: 34418508 DOI: 10.1016/j.neulet.2021.136183] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2021] [Revised: 08/14/2021] [Accepted: 08/16/2021] [Indexed: 10/20/2022]
Abstract
BACKGROUND Mitragynine, the major indole alkaloid from Mitragyna speciosa has been reported previously to possess abuse liability. However, there are insufficient data suggesting the mechanism through which this pharmacological agent causes addiction. AIMS In this study, we investigated the effects of mitragynine on dopamine (DA) level and dopamine transporter (DAT) expression from the rat's frontal cortex. METHODS DA level was recorded in the brain samples of animals treated with acute or repeated exposure for 4 consecutive days with either vehicle or mitragynine (1 and 30 mg/kg) using electrochemical sensor. Animals were then decapitated and the brain regions were removed, snap-frozen in liquid nitrogen and immediately stored at -80 °C. DA level was quantified using Enzyme linked immunosorbent assay (ELISA) kits and DAT gene expression was determined using quantitative real time polymerase chain reaction (RT-qPCR). RESULTS/OUTCOME Mitragynine (1 and 30 mg/kg) did not increase DA release following acute treatment, however, after repeated exposure at day 4, mitragynine significantly and dose dependently increased DA release in the frontal cortex. In this study, we also observed a significant increase in DAT mRNA expression at day 4 in group treated with mitragynine (30 mg/kg). CONCLUSION/INTERPRETATION Data from this study indicates that mitragynine significantly increased DA release when administered repeatedly, increased in DAT mRNA expression with the highest tested dose (30 mg/kg). Therefore, the rewarding effects observed after mitragynine administration could be due to its ability to increase DA content in certain areas of the brain especially the frontal cortex.
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Affiliation(s)
| | - Suleiman Yunusa
- Centre for Drug Research, Universiti Sains Malaysia, 11800 Penang, Malaysia; Department of Pharmacology, Bauchi State University Gadau, PMB 65, Bauchi State, Nigeria
| | - Zainiharyati M Zain
- Electrochemical Material and Sensor Laboratory, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
| | - Zurina Hassan
- Centre for Drug Research, Universiti Sains Malaysia, 11800 Penang, Malaysia.
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Sensing Methods for Hazardous Phenolic Compounds Based on Graphene and Conducting Polymers-Based Materials. CHEMOSENSORS 2021. [DOI: 10.3390/chemosensors9100291] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Abstract
It has been known for years that the phenolic compounds are able to exert harmful effects toward living organisms including humans due to their high toxicity. Living organisms were exposed to these phenolic compounds as they were released into the environment as waste products from several fast-growing industries. In this regard, tremendous efforts have been made by researchers to develop sensing methods for the detection of these phenolic compounds. Graphene and conducting polymers-based materials have arisen as a high potential sensing layer to improve the performance of the developed sensors. Henceforth, this paper reviews the existing investigations on graphene and conducting polymer-based materials incorporated with various sensors that aimed to detect hazardous phenolic compounds, i.e., phenol, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrophenol, and 2,4-dimethylphenol. The whole picture and up-to-date information on the graphene and conducting polymers-based sensors are arranged in systematic chronological order to provide a clearer insight in this research area. The future perspectives of this study are also included, and the development of sensing methods for hazardous phenolic compounds using graphene and conducting polymers-based materials is expected to grow more in the future.
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Post MR, Sulzer D. The chemical tools for imaging dopamine release. Cell Chem Biol 2021; 28:748-764. [PMID: 33894160 PMCID: PMC8532025 DOI: 10.1016/j.chembiol.2021.04.005] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/11/2020] [Revised: 02/23/2021] [Accepted: 04/06/2021] [Indexed: 02/07/2023]
Abstract
Dopamine is a modulatory neurotransmitter involved in learning, motor functions, and reward. Many neuropsychiatric disorders, including Parkinson's disease, autism, and schizophrenia, are associated with imbalances or dysfunction in the dopaminergic system. Yet, our understanding of these pervasive public health issues is limited by our ability to effectively image dopamine in humans, which has long been a goal for chemists and neuroscientists. The last two decades have witnessed the development of many molecules used to trace dopamine. We review the small molecules, nanoparticles, and protein sensors used with fluorescent microscopy/photometry, MRI, and PET that shape dopamine research today. None of these tools observe dopamine itself, but instead harness the biology of the dopamine system-its synthetic and metabolic pathways, synaptic vesicle cycle, and receptors-in elegant ways. Their advantages and weaknesses are covered here, along with recent examples and the chemistry and biology that allow them to function.
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Affiliation(s)
- Michael R Post
- Department of Psychiatry, Columbia University Medical Center, New York, NY, USA; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, USA.
| | - David Sulzer
- Departments of Psychiatry, Neurology, and Pharmacology, Columbia University Medical Center, New York, NY, USA; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, USA.
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Fauzi NIM, Fen YW, Omar NAS, Hashim HS. Recent Advances on Detection of Insecticides Using Optical Sensors. SENSORS (BASEL, SWITZERLAND) 2021; 21:3856. [PMID: 34204853 PMCID: PMC8199770 DOI: 10.3390/s21113856] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/14/2021] [Revised: 04/22/2021] [Accepted: 04/24/2021] [Indexed: 02/07/2023]
Abstract
Insecticides are enormously important to industry requirements and market demands in agriculture. Despite their usefulness, these insecticides can pose a dangerous risk to the safety of food, environment and all living things through various mechanisms of action. Concern about the environmental impact of repeated use of insecticides has prompted many researchers to develop rapid, economical, uncomplicated and user-friendly analytical method for the detection of insecticides. In this regards, optical sensors are considered as favorable methods for insecticides analysis because of their special features including rapid detection time, low cost, easy to use and high selectivity and sensitivity. In this review, current progresses of incorporation between recognition elements and optical sensors for insecticide detection are discussed and evaluated well, by categorizing it based on insecticide chemical classes, including the range of detection and limit of detection. Additionally, this review aims to provide powerful insights to researchers for the future development of optical sensors in the detection of insecticides.
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Affiliation(s)
- Nurul Illya Muhamad Fauzi
- Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; (N.I.M.F.); (N.A.S.O.)
| | - Yap Wing Fen
- Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; (N.I.M.F.); (N.A.S.O.)
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;
| | - Nur Alia Sheh Omar
- Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; (N.I.M.F.); (N.A.S.O.)
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;
| | - Hazwani Suhaila Hashim
- Department of Physics, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;
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Tertis M, Hosu O, Feier B, Cernat A, Florea A, Cristea C. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules 2021; 26:3200. [PMID: 34071841 PMCID: PMC8198121 DOI: 10.3390/molecules26113200] [Citation(s) in RCA: 20] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/15/2021] [Revised: 05/22/2021] [Accepted: 05/24/2021] [Indexed: 12/23/2022] Open
Abstract
Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.
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Affiliation(s)
| | | | | | | | | | - Cecilia Cristea
- Department of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania; (M.T.); (O.H.); (B.F.); (A.C.); (A.F.)
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Cernat A, Ştefan G, Tertis M, Cristea C, Simon I. An overview of the detection of serotonin and dopamine with graphene-based sensors. Bioelectrochemistry 2020; 136:107620. [DOI: 10.1016/j.bioelechem.2020.107620] [Citation(s) in RCA: 24] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/13/2020] [Revised: 07/23/2020] [Accepted: 07/23/2020] [Indexed: 02/06/2023]
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Arkusz K, Paradowska E. Impedimetric Detection of Femtomolar Levels of Interleukin6, Interleukin 8, and Tumor Necrosis Factor Alpha Based on Thermally Modified Nanotubular Titanium Dioxide Arrays. NANOMATERIALS (BASEL, SWITZERLAND) 2020; 10:E2399. [PMID: 33266223 PMCID: PMC7760759 DOI: 10.3390/nano10122399] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/09/2020] [Revised: 11/23/2020] [Accepted: 11/30/2020] [Indexed: 01/07/2023]
Abstract
An inexpensive, easy to prepare, and label-free electrochemical impedance spectroscopy-based biosensor has been developed for the selective detection of human interleukin 6 (IL-6), interleukin 8 (CXCL8, IL-8), and tumor necrosis factor (TNFα)-potential inflammatory cancer biomarkers. We describe a, so far, newly developed and unexplored method to immobilize antibodies onto a titanium dioxide nanotube (TNT) array by physical adsorption. Immobilization of anti-IL-6, anti-IL-8, and anti-TNFα on TNT and the detection of human IL-6, IL-8, and TNFα were examined using electrochemical impedance spectroscopy (EIS). The impedimetric immunosensor demonstrates good selectivity and high sensitivity against human biomarker analytes and can detect IL-6, IL-8, and TNFα at concentrations as low as 5 pg/mL, equivalent to the standard concentration of these proteins in human blood. The calibration curves evidenced that elaborated biosensors are sensitive to three cytokines within 5 ÷ 2500 pg/mL in the 0.01 M phosphate-buffered saline solution (pH 7.4).
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Affiliation(s)
- Katarzyna Arkusz
- Department of Biomedical Engineering, Faculty of Mechanical Engineering, University of Zielona Gora, Licealna 9 Street, 65-417 Zielona Gora, Poland;
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A 433-MHz surface acoustic wave sensor with Ni-TiO 2-poly(L-lysine) composite film for dopamine determination. Mikrochim Acta 2020; 187:671. [PMID: 33225378 DOI: 10.1007/s00604-020-04635-7] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/28/2020] [Accepted: 11/06/2020] [Indexed: 01/30/2023]
Abstract
A ternary hybrid material composed of Ni nanoparticles (NPs), TiO2 NPs, and poly(L-lysine) (Ply) was used as a sensing material. It was electrodeposited in situ onto a commercial 433-MHz surface acoustic wave (SAW) resonator to construct a Ni-TiO2-Ply/SAW sensor. The Ni-TiO2-Ply sensing layer fully covered the resonant cavity of the SAW resonator. As the sensing layer completely covers the interdigital transducer and piezoelectric substrate, the sensing area is significantly increased, and the resonator is protected from damage or contamination. To detect the level of dopamine (DA) in serum, the fabrication of the Ni-TiO2-Ply sensing layer, distributions of various components in the sensing layer, and responses of the SAW biosensor to DA were investigated in detail. In addition, an electric field-assisted liquid-phase oxidation technique was developed for loading analytes onto the SAW sensors. After optimizing the pH value and L-lysine content of the sensing layer electrolyte and the pH value of the DA solution, the SAW biosensor responded to DA with a linear concentration range of 1 to 1000 nM, sensitivity of 5.77 MHz nM-1 cm-2, and limit of detection of 0.067 nM. Moreover, the sensor exhibited good selectivity, reproducibility, and stability at ambient temperature.Graphical abstract.
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Liu X, Hou Y, Chen S, Liu J. Controlling dopamine binding by the new aptamer for a FRET-based biosensor. Biosens Bioelectron 2020; 173:112798. [PMID: 33197768 DOI: 10.1016/j.bios.2020.112798] [Citation(s) in RCA: 33] [Impact Index Per Article: 6.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/09/2020] [Revised: 10/19/2020] [Accepted: 11/02/2020] [Indexed: 12/11/2022]
Abstract
Dopamine is one of the most important neurotransmitters. A high-quality DNA aptamer for dopamine was reported in 2018. However, fundamental understanding of its binding and folding is lacking, which is critical for related biosensor design. Herein, we performed careful assays using a label-free technique called isothermal titration calorimetry (ITC) to study its secondary structure. We divided this aptamer into four regions and individually examined each of them. We confirmed two stems, but the third stem is believed to be part of a loop. The aptamer was then truncated. The original aptamer had a Kd of 2.2 ± 0.3 μM at 25 °C. Shortening the structure by one or two base pairs increased the Kd to 6.9 and 44.4 μM, respectively. Dopamine binding was promoted by both increasing the Mg2+ concentration and decreasing the temperature. At 5 °C, a Kd of 0.4 μM was achieved. Based on this understanding, we designed two fluorescence resonance energy transfer (FRET) quenching biosensors that differ only by a base pair. The shorter sensor had 3-fold higher sensitivity and a detection limit of 0.9 μM. In 1% fetal bovine serum, the sensor retained a similar limit of detection of 1.14 μM. A two-fluorophore ratiometric FRET sensor was also demonstrated with a low detection limit of 0.12 μM. This work indicated the feasibility of designing folding-based sensors for dopamine, and this design can be extended to other sensing modalities such as electrochemistry and colorimetry.
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Affiliation(s)
- Xixia Liu
- Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, Hubei province, 435002, China; Department of Chemistry, and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada
| | - Yaoyao Hou
- Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, Hubei province, 435002, China
| | - Sirui Chen
- Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, Hubei province, 435002, China
| | - Juewen Liu
- Department of Chemistry, and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
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48
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Affiliation(s)
- Xixia Liu
- Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization Hubei Normal University Huangshi China
- Department of Chemistry and Waterloo Institute for Nanotechnology University of Waterloo Waterloo Canada
| | - Juewen Liu
- Department of Chemistry and Waterloo Institute for Nanotechnology University of Waterloo Waterloo Canada
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Díaz-Liñán MC, García-Valverde MT, Lucena R, Cárdenas S, López-Lorente AI. Paper-based sorptive phases for microextraction and sensing. ANALYTICAL METHODS : ADVANCING METHODS AND APPLICATIONS 2020; 12:3074-3091. [PMID: 32930167 DOI: 10.1039/d0ay00702a] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/11/2023]
Abstract
The simplification of the analytical procedures, including cost-effective materials and detectors, is a current research trend. In this context, paper has been identified as a useful material thanks to its low price and high availability in different compositions (office, filter, chromatographic). Its porosity, flexibility, and planar geometry permit the design of flow-through devices compatible with most instrumental techniques. This article provides a general overview of the potential of paper, as substrate, on the simplification of analytical chemistry methodologies. The design of paper-based sorptive phases is considered in-depth, and the different functionalization strategies are described. Considering our experience in sample preparation, special attention has been paid to the use of these phases under the classical microextraction-analysis workflow, which usually includes a chromatographic separation of the analytes before their determination. However, the interest of these materials extends beyond this field as they can be easily implemented into spectroscopic and electrochemical sensors. Finally, the direct analysis of paper substrates in mass spectrometry, in the so-called paper-spray technique is also discussed. This review is more focused on presenting ideas rather than the description of specific applications to draw a general picture of the potential of these materials.
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Affiliation(s)
- M C Díaz-Liñán
- Departamento de Química Analítica, Instituto, Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071 Córdoba, Spain.
| | - M T García-Valverde
- Departamento de Química Analítica, Instituto, Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071 Córdoba, Spain.
| | - R Lucena
- Departamento de Química Analítica, Instituto, Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071 Córdoba, Spain.
| | - S Cárdenas
- Departamento de Química Analítica, Instituto, Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071 Córdoba, Spain.
| | - A I López-Lorente
- Departamento de Química Analítica, Instituto, Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071 Córdoba, Spain.
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Stone Paper as a New Substrate to Fabricate Flexible Screen-Printed Electrodes for the Electrochemical Detection of Dopamine. SENSORS 2020; 20:s20123609. [PMID: 32604924 PMCID: PMC7349771 DOI: 10.3390/s20123609] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/25/2020] [Revised: 06/16/2020] [Accepted: 06/23/2020] [Indexed: 01/19/2023]
Abstract
Flexible screen-printed electrodes (HP) were fabricated on stone paper substrate and amperometrically modified with gold nanoparticles (HP-AuNPs). The modified electrode displayed improved electronic transport properties, reflected in a low charge-transfer resistance (1220 Ω) and high apparent heterogeneous electron transfer rate constant (1.94 × 10−3 cm/s). The voltammetric detection of dopamine (DA) was tested with HP and HP-AuNPs electrodes in standard laboratory solutions (pH 6 phosphate-buffered saline (PBS)) containing various concentrations of analyte (10−7–10−3 M). As expected, the modified electrode exhibits superior performances in terms of linear range (10−7–10−3 M) and limit of detection (3 × 10−8 M), in comparison with bare HP. The determination of DA was tested with HP-AuNPs in spiked artificial urine and in pharmaceutical drug solution (ZENTIVA) that contained dopamine hydrochloride (5 mg/mL). The results obtained indicated a very good DA determination in artificial urine without significant matrix effects. In the case of the pharmaceutical drug solution, the DA determination was affected by the interfering species present in the vial, such as sodium metabisulfite, maleic acid, sodium chloride, and propylene glycol.
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