GadE regulates fliC gene transcription and motility in Escherichia coli

doi:10.5495/wjcid.v10.i1.14

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May 15, 2020 (publication date) through Jul 26, 2024

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World Journal of Clinical Infectious Diseases

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2220-3176

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Clin Infect Dis. May 15, 2020; 10(1): 14-23
Published online May 15, 2020. doi: 10.5495/wjcid.v10.i1.14

Table 3 Effect of osmolarity on fliC::lacZ gene transcription in Escherichia coli grown in buffered pH 5.5 and 7.0 Luria Bertani media with different osmolarities

E. coli strain	NaCl (mmol/L)	Gal activity¹
E. coli strain	NaCl (mmol/L)	pH 5.5	pH 7.0
MC4100/pNK2-29	0	308 ± 104²	1132 ± 130
MC4100/pNK2-29	100	338 ± 128	806 ± 41
MC4100/pNK2-29	200	251 ± 68.5	689 ± 173
MC4100/pNK2-29	400	62 ± 22.0	454 ± 71
NU149 LacZ1/pNK2-29	0	442 ± 72	1353 ± 98
NU149 LacZ1/pNK2-29	100	418 ± 61	976 ± 52
NU149 LacZ1/pNK2-29	200	293 ± 43	811 ± 75
NU149 LacZ1/pNK2-29	400	147 ± 39	489 ± 61

¹Galactosidase activity measured as Miller units.

²Data represents the mean ± standard deviation from three separate runs.

Citation: Schwan WR, Flohr NL, Multerer AR, Starkey JC. GadE regulates fliC gene transcription and motility in Escherichia coli. World J Clin Infect Dis 2020; 10(1): 14-23
URL: https://www.wjgnet.com/2220-3176/full/v10/i1/14.htm
DOI: https://dx.doi.org/10.5495/wjcid.v10.i1.14