|
1
|
Rosenberg M, Park S, Umerov S, Ivask A. Experimental evolution of Escherichia coli on semi-dry silver, copper, stainless steel, and glass surfaces. Microbiol Spectr 2025; 13:e0217324. [PMID: 39948723 PMCID: PMC11960088 DOI: 10.1128/spectrum.02173-24] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/02/2024] [Accepted: 12/23/2024] [Indexed: 04/03/2025] Open
Abstract
To study bacterial adaptation to antimicrobial metal surfaces in application-relevant conditions, Escherichia coli was exposed to copper and silver surfaces for 30 exposure cycles in low-organic dry or high-organic humid conditions. The evolved populations demonstrated increased metal surface tolerance without concurrent increase in minimal biocidal concentration (MBC) and minimal inhibitory concentration (MIC) values of respective metal ions or selected antibiotics. Mutation analysis did not detect increased mutation accumulation nor mutations in cop, cus, cue, sil, pco, or general efflux genes known to actively maintain copper/silver homeostasis. Instead, during cyclic exposure, mutations in genes related to cellular barrier functions and sulfur metabolism were enriched, potentially suggesting that reducing bioavailability and passively restricting uptake of the toxic metals rather than active efflux is selected for on copper and silver surfaces. The changes detected in the evolved populations did not indicate an increased risk of antibiotic cross-resistance as a result of copper or silver surface exposure. However, rapid emergence of mutations in silS activated the cryptic sil efflux locus during silver ion challenge in liquid MBC assay with the evolved populations. The silS mutants showed no benefit on copper and silver surfaces but demonstrated decreased sensitivity to ampicillin and ciprofloxacin, as well as copper and silver ions in liquid tests, indicating that efflux might be specific to granting heavy metal tolerance in liquid but not surface exposure format. Our findings highlight the critical importance of appropriate exposure conditions not only in efficacy testing but also in risk assessment of antimicrobial surface applications. IMPORTANCE This study examines the evolutionary adaptations of Escherichia coli after semi-dry exposure to copper and silver surfaces, leading to an increase in surface tolerance but no increase in mutation accumulation or substantially enhanced metal ion tolerance in standard tests. Notably, enriched mutations indicate a shift toward more energy-passive mechanisms of metal tolerance. Additionally, while enhanced silver efflux was rapidly selected for in a single round of silver exposure in liquid tests and substantially increased copper and silver ion tolerance in conventional test formats, the causal mutations did not improve viability on silver and copper surfaces, underscoring the different fitness scenarios of tolerance mechanisms dependent on exposure conditions. These findings emphasize the need for appropriate exposure conditions in evaluating of both efficacy and the potential risks of using antimicrobial surfaces, as the results from conventional liquid-based tests may not apply in solid contexts.
Collapse
Affiliation(s)
- Merilin Rosenberg
- Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
| | - Sandra Park
- Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
| | - Sigrit Umerov
- Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
| | - Angela Ivask
- Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
| |
Collapse
|
|
2
|
Müller DW, Pauly C, Brix K, Kautenburger R, Mücklich F. Modifying the antibacterial performance of Cu surfaces by topographic patterning in the micro- and nanometer scale. BIOMATERIALS ADVANCES 2025; 169:214184. [PMID: 39813739 DOI: 10.1016/j.bioadv.2025.214184] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/05/2024] [Revised: 01/07/2025] [Accepted: 01/09/2025] [Indexed: 01/18/2025]
Abstract
Antimicrobial surfaces are a promising approach to reduce the spread of pathogenic microorganisms in various critical environments. To achieve high antimicrobial functionality, it is essential to consider the material-specific bactericidal mode of action in conjunction with bacterial surface interactions. This study investigates the effect of altered contact conditions on the antimicrobial efficiency of Cu surfaces against Escherichia coli and Staphylococcus aureus. The fabrication of line-like periodic surface patterns in the scale range of single bacterial cells was achieved utilizing ultrashort pulsed direct laser interference patterning. These patterns create both favorable and unfavorable topographies for bacterial adhesion. The variation in bacteria/surface interaction is monitored in terms of strain-specific bactericidal efficiency and the role of corrosive forces driving quantitative Cu ion release. The investigation revealed that bacterial deactivation on Cu surfaces can be either enhanced or decreased by intentional topography modifications, independent of Cu ion emission, with strain-specific deviations in effective pattern scales observed. The results of this study indicate the potential of targeted topographic surface functionalization to optimize antimicrobial surface designs, enabling strain-specific decontamination strategies.
Collapse
Affiliation(s)
- Daniel Wyn Müller
- Chair of Functional Materials, Department of Materials Science, Saarland University, 66123 Saarbrücken, Germany; SurFunction GmbH, 66123 Saarbrücken, Germany.
| | - Christoph Pauly
- Chair of Functional Materials, Department of Materials Science, Saarland University, 66123 Saarbrücken, Germany
| | - Kristina Brix
- Department of Inorganic Solid-State Chemistry, Elemental Analysis, Saarland University, 66123 Saarbrücken, Germany
| | - Ralf Kautenburger
- Department of Inorganic Solid-State Chemistry, Elemental Analysis, Saarland University, 66123 Saarbrücken, Germany
| | - Frank Mücklich
- Chair of Functional Materials, Department of Materials Science, Saarland University, 66123 Saarbrücken, Germany
| |
Collapse
|
|
3
|
Zhang Q, Zong Q, Feng X, Luo M, Sun W, Zhai Y. Antibacterial and antifouling materials for urinary catheter coatings. Acta Biomater 2025; 192:28-47. [PMID: 39701341 DOI: 10.1016/j.actbio.2024.12.040] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/27/2024] [Revised: 11/15/2024] [Accepted: 12/16/2024] [Indexed: 12/21/2024]
Abstract
Implantable medical devices have played a significant role in improving both medical care and patients' quality of life. Urinary Catheters (UCs) are commonly utilized as a substitute for bladder drainage and urine collection to prevent urinary retention in patients. However, bacterial colonization and biofilm formation on the catheter surface are prone to occur, leading to catheter-associated urinary tract infections (CAUTIs) and other complications. In recent years, UC coatings have garnered increasing attention. In this review, various antifouling and antibacterial materials for UC coatings are summarized and their impacts on bacterial activities are linked to potential mechanisms of action. Additionally, this review provides an in-depth understanding of the current advancements in UC coatings by presenting the advantages, limitations, notable achievements, and latest research findings. Finally, it anticipates the prospective design and development trajectories of UC coatings in this domain. This holds paramount significance in advancing medical device technology. STATEMENT OF SIGNIFICANCE: Combating catheter-associated urinary tract infections is a major healthcare challenge, and urinary catheter (UC) coatings are considered promising candidates to counter these infections. In this review, various antifouling and antibacterial materials for UCs are summarized, and their impacts on bacterial activities are linked to potential mechanisms of action. Additionally, the review provides an in-depth understanding of the current advancements in UC coatings by presenting the advantages, limitations, notable achievements, and latest research findings. This holds paramount significance in advancing medical device technology. This review not only contributes to the scientific research but also sparks interest among readerships and other researchers in the study of safer and more effective UC coatings for improved patient outcomes.
Collapse
Affiliation(s)
- Qianwen Zhang
- Department of Biomedical Engineering, School of Pharmaceutical University, Shenyang 110016, China
| | - Qida Zong
- Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China
| | - Xinke Feng
- Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China
| | - Min Luo
- Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China
| | - Wei Sun
- Department of Biomedical Engineering, School of Pharmaceutical University, Shenyang 110016, China.
| | - Yinglei Zhai
- Department of Biomedical Engineering, School of Pharmaceutical University, Shenyang 110016, China.
| |
Collapse
|
|
4
|
Lorenzetti L, Brandolini M, Gatti G, Bernardi E, Chiavari C, Gualandi P, Galliani G, Sambri V, Martini C. Cu-based thin rolled foils: relationship among alloy composition, micromechanical and antiviral properties against SARS-CoV-2. Heliyon 2024; 10:e28238. [PMID: 38560697 PMCID: PMC10979200 DOI: 10.1016/j.heliyon.2024.e28238] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/08/2024] [Revised: 03/13/2024] [Accepted: 03/14/2024] [Indexed: 04/04/2024] Open
Abstract
The healthcare-associated infections (HAIs) and pandemics caused by multidrug-resistant (MDR) and new-generation pathogens threaten the whole world community. Cu and its alloys have been attracting widespread interest as anti-contamination materials due to the rapid inactivation of MDR-superbugs and viruses. Applying thin Cu-based foils on pre-existing surfaces in hygiene-sensitive areas represents a quick, simple, cost-effective self-sanitising practice. However, the influence of chemical composition and microstructure should be deeply investigated when evaluating the antimicrobial capability and durability of Cu-based materials. The effect of composition on micromechanical and antiviral properties was investigated by comparing Cu15Zn and Cu18Ni20Zn (foil thickness from 13 to 27 μm) with Phosphorous High-Conductivity (PHC) Cu. The influence of recrystallisation annealing of PHC Cu was also investigated. Microstructural characterisation was carried out by optical (OM) and scanning electron (FEG-SEM) microscopy, Energy-dispersive Spectroscopy (EDS) and Electron-Backscattered Diffraction (EBSD). The micromechanical behaviour was assessed by microhardness, microscale abrasion and scratch tests. Cu-based foils were exposed to SARS-CoV-2 for different time points in quasi-dry conditions (artificial sweat solution), evaluating their antiviral capability by quantitative Reverse-Transcriptase Polymerase Chain Reaction (qRT-PCR). Surface morphology, contact angle measurements and Cu release were measured. All Cu-based surfaces completely inactivated SARS-CoV-2 in 10 min: pure Cu was the best option regarding antiviral efficiency, while Cu15Zn showed the best trade-off between micromechanical and antiviral properties.
Collapse
Affiliation(s)
- L. Lorenzetti
- Dept. Industrial Engineering (DIN), University of Bologna, Viale Risorgimento 4, 40136 Bologna, Italy
| | - M. Brandolini
- Unit of Microbiology, The Great Romagna Hub Laboratory, Piazza della Liberazione 60, 47522 Pievesestina, Italy
- Dept. Medical and Surgical Sciences (DIMEC), University of Bologna, Via Irnerio 49, 40126 Bologna, Italy
| | - G. Gatti
- Unit of Microbiology, The Great Romagna Hub Laboratory, Piazza della Liberazione 60, 47522 Pievesestina, Italy
- Dept. Medical and Surgical Sciences (DIMEC), University of Bologna, Via Irnerio 49, 40126 Bologna, Italy
| | - E. Bernardi
- Dept. Industrial Chemistry "Toso Montanari", University of Bologna, Via Piero Gobetti 85, Bologna 40129, Italy
| | - C. Chiavari
- Dept. Cultural Heritage (DBC), University of Bologna, Via degli Ariani 1, 48121 Ravenna, Italy
| | - P. Gualandi
- Pietro Galliani SpA, Via Molino Malpasso 65, 40038 Vergato (BO), Italy
| | - G. Galliani
- Pietro Galliani SpA, Via Molino Malpasso 65, 40038 Vergato (BO), Italy
| | - V. Sambri
- Unit of Microbiology, The Great Romagna Hub Laboratory, Piazza della Liberazione 60, 47522 Pievesestina, Italy
- Dept. Medical and Surgical Sciences (DIMEC), University of Bologna, Via Irnerio 49, 40126 Bologna, Italy
| | - C. Martini
- Dept. Industrial Engineering (DIN), University of Bologna, Viale Risorgimento 4, 40136 Bologna, Italy
| |
Collapse
|
|
5
|
Guo K, Chen C. Investigation of Far-UVC (222 nm) disinfection of bioaerosols deposited on surfaces with different material properties. JOURNAL OF HAZARDOUS MATERIALS 2024; 465:133358. [PMID: 38157810 DOI: 10.1016/j.jhazmat.2023.133358] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/13/2023] [Revised: 12/08/2023] [Accepted: 12/21/2023] [Indexed: 01/03/2024]
Abstract
Far-ultraviolet C (UVC) light has demonstrated its ability to inactivate microbes on surfaces. However, the factors influencing the efficacy of far-UVC surface disinfection remain unclear. This study aimed to explore the effects of material properties on far-UVC disinfection of bioaerosols (represented by Escherichia coli (E. coli)) deposited on surfaces. The susceptibility constants (Z-values) of E. coli on 14 common materials were measured and analyzed. Additionally, five possible influencing factors (roughness, pores, electrostatic charge, wetness, and temperature) related to surface properties were investigated by control experiments. The results show that far-UVC light effectively disinfected E. coli on the 14 materials, with disinfection efficiencies ranging from 69.1% to 98.9% under a dose of 100.8 J/m2. Surface roughness and electrostatic charges had negligible influence on far-UVC disinfection of E. coli on surfaces. However, for porous materials, pore sizes larger than the E. coli size resulted in lower Z-values. Higher surface wetness decreased both the Z-value and natural decay rate. Meanwhile, a higher surface temperature of 40 °C resulted in a higher Z-value and natural decay rate. The results can improve our understanding of far-UVC disinfection of microbes on surfaces, and the database can be used for numerical models.
Collapse
Affiliation(s)
- Kangqi Guo
- Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, NT 999077, Hong Kong SAR, China
| | - Chun Chen
- Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, NT 999077, Hong Kong SAR, China; Institute of Environment, Energy and Sustainability, The Chinese University of Hong Kong, Shatin, NT 999077, Hong Kong SAR, China.
| |
Collapse
|
|
6
|
Ramos-Zúñiga J, Bruna N, Pérez-Donoso JM. Toxicity Mechanisms of Copper Nanoparticles and Copper Surfaces on Bacterial Cells and Viruses. Int J Mol Sci 2023; 24:10503. [PMID: 37445681 DOI: 10.3390/ijms241310503] [Citation(s) in RCA: 29] [Impact Index Per Article: 14.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/24/2023] [Revised: 06/08/2023] [Accepted: 06/13/2023] [Indexed: 07/15/2023] Open
Abstract
Copper is a metal historically used to prevent infections. One of the most relevant challenges in modern society are infectious disease outbreaks, where copper-based technologies can play a significant role. Currently, copper nanoparticles and surfaces are the most common antimicrobial copper-based technologies. Despite the widespread use of copper on nanoparticles and surfaces, the toxicity mechanism(s) explaining their unique antimicrobial properties are not entirely known. In general, toxicity effects described in bacteria and fungi involve the rupture of membranes, accumulation of ions inside the cell, protein inactivation, and DNA damage. A few studies have associated Cu-toxicity with ROS production and genetic material degradation in viruses. Therefore, understanding the mechanisms of the toxicity of copper nanoparticles and surfaces will contribute to developing and implementing efficient antimicrobial technologies to combat old and new infectious agents that can lead to disease outbreaks such as COVID-19. This review summarizes the current knowledge regarding the microbial toxicity of copper nanoparticles and surfaces and the gaps in this knowledge. In addition, we discuss potential applications derived from discovering new elements of copper toxicity, such as using different molecules or modifications to potentiate toxicity or antimicrobial specificity.
Collapse
Affiliation(s)
- Javiera Ramos-Zúñiga
- BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370186, Chile
| | - Nicolás Bruna
- BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370186, Chile
| | - José M Pérez-Donoso
- BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370186, Chile
| |
Collapse
|
|
7
|
Jeong SB, Shin JH, Kim SW, Seo SC, Jung JH. Performance evaluation of an electrostatic precipitator with a copper plate using an aerosolized SARS-CoV-2 surrogate (bacteriophage phi 6). ENVIRONMENTAL TECHNOLOGY & INNOVATION 2023; 30:103124. [PMID: 36987524 PMCID: PMC10035800 DOI: 10.1016/j.eti.2023.103124] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 11/06/2022] [Revised: 01/29/2023] [Accepted: 03/21/2023] [Indexed: 06/19/2023]
Abstract
The global spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has reminded us of the importance of developing technologies to reduce and control bioaerosols in built environments. For bioaerosol control, the interaction between researchers and biomaterials is essential, and considering the characteristics of target pathogens is strongly required. Herein, we used enveloped viral aerosols, bacteriophage phi 6, for evaluating the performance of an electrostatic precipitator (ESP) with a copper-collecting plate (Cu-plate). In particular, bacteriophage phi 6 is an accessible enveloped virus that can be operated in biosafety level (BSL)-1 as a promising surrogate for SARS-CoV-2 with structural and morphological similarities. ESP with Cu-plate showed >91% of particle removal efficiency for viral aerosols at 77 cm/s of airflow face velocity. Moreover, the Cu-plate presented a potent antiviral performance of 5.4-relative log reduction within <15 min of contact. We believe that the evaluation of ESP performance using an aerosolized enveloped virus and plaque assay is invaluable. Our results provide essential information for the development of bioaerosol control technologies that will lead the post-corona era.
Collapse
Affiliation(s)
- Sang Bin Jeong
- Department of Mechanical Engineering, Sejong University, Seoul 05006, Republic of Korea
| | - Jae Hak Shin
- Department of Mechanical Engineering, Sejong University, Seoul 05006, Republic of Korea
| | - Sam Woong Kim
- Agri-Food Bio Convergence Institute, Gyeongsang National University, Jinju 52725, Republic of Korea
| | - Sung Chul Seo
- Department of Nano, Chemical and Biological Engineering, Seokyeong University, Seoul 02713, Republic of Korea
| | - Jae Hee Jung
- Department of Mechanical Engineering, Sejong University, Seoul 05006, Republic of Korea
| |
Collapse
|
|
8
|
Norambuena J, Al-Tameemi H, Bovermann H, Kim J, Beavers WN, Skaar EP, Parker D, Boyd JM. Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus. PLoS Pathog 2023; 19:e1011393. [PMID: 37235600 PMCID: PMC10249872 DOI: 10.1371/journal.ppat.1011393] [Citation(s) in RCA: 9] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/06/2023] [Revised: 06/08/2023] [Accepted: 04/27/2023] [Indexed: 05/28/2023] Open
Abstract
To gain a better insight of how Copper (Cu) ions toxify cells, metabolomic analyses were performed in S. aureus strains that lacks the described Cu ion detoxification systems (ΔcopBL ΔcopAZ; cop-). Exposure of the cop- strain to Cu(II) resulted in an increase in the concentrations of metabolites utilized to synthesize phosphoribosyl diphosphate (PRPP). PRPP is created using the enzyme phosphoribosylpyrophosphate synthetase (Prs) which catalyzes the interconversion of ATP and ribose 5-phosphate to PRPP and AMP. Supplementing growth medium with metabolites requiring PRPP for synthesis improved growth in the presence of Cu(II). A suppressor screen revealed that a strain with a lesion in the gene coding adenine phosphoribosyltransferase (apt) was more resistant to Cu. Apt catalyzes the conversion of adenine with PRPP to AMP. The apt mutant had an increased pool of adenine suggesting that the PRPP pool was being redirected. Over-production of apt, or alternate enzymes that utilize PRPP, increased sensitivity to Cu(II). Increasing or decreasing expression of prs resulted in decreased and increased sensitivity to growth in the presence of Cu(II), respectively. We demonstrate that Prs is inhibited by Cu ions in vivo and in vitro and that treatment of cells with Cu(II) results in decreased PRPP levels. Lastly, we establish that S. aureus that lacks the ability to remove Cu ions from the cytosol is defective in colonizing the airway in a murine model of acute pneumonia, as well as the skin. The data presented are consistent with a model wherein Cu ions inhibits pentose phosphate pathway function and are used by the immune system to prevent S. aureus infections.
Collapse
Affiliation(s)
- Javiera Norambuena
- Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States of America
| | - Hassan Al-Tameemi
- Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States of America
| | - Hannah Bovermann
- Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States of America
| | - Jisun Kim
- Department of Pathology, Immunology and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, New Jersey, United States of America
| | - William N. Beavers
- Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
| | - Eric P. Skaar
- Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
| | - Dane Parker
- Department of Pathology, Immunology and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, New Jersey, United States of America
| | - Jeffrey M. Boyd
- Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States of America
| |
Collapse
|
|
9
|
Vanheuverzwijn J, Maillard EE, Mahat A, Fowler L, Monteyne D, Bonnaud L, Landercy N, Hemberg A, Janković A, Meyer F, Mišković-Stanković V, Stevanović M, Mirica C, Pérez-Morga D, Luginbuehl R, Combes C, Furtos G, Fontaine V. Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. Microorganisms 2023; 11:microorganisms11041023. [PMID: 37110446 PMCID: PMC10146976 DOI: 10.3390/microorganisms11041023] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/06/2023] [Revised: 03/29/2023] [Accepted: 04/11/2023] [Indexed: 04/29/2023] Open
Abstract
Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
Collapse
Affiliation(s)
- Jérome Vanheuverzwijn
- Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Eloise-Eliane Maillard
- Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Amal Mahat
- Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Lee Fowler
- Applied Materials Science, The Ångström Laboratory, Department of Engineering Sciences, Uppsala University, P.O. Box 534, 75121 Uppsala, Sweden
| | - Daniel Monteyne
- Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium
| | - Leïla Bonnaud
- Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium
| | - Nicolas Landercy
- Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium
| | - Axel Hemberg
- Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium
| | - Ana Janković
- Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
| | - Franck Meyer
- Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
| | - Vesna Mišković-Stanković
- Faculty of Ecology and Environmental Protection, University Union-Nikola Tesla, Cara Dusana 62-64, 11158 Belgrade, Serbia
| | - Milena Stevanović
- Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
| | - Codruta Mirica
- Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street 15, 400012 Cluj-Napoca, Romania
| | - David Pérez-Morga
- Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium
| | - Reto Luginbuehl
- Department of Biomedical Material Research, University of Bern, 3008 Bern, Switzerland
- Blaser Swisslube, 3415 Hasle-Rüegsau, Switzerland
| | - Christèle Combes
- Centre Inter-Universitaire de Recherche et d'Ingénierie des Matériaux, CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, 4 allée Emile Monso, BP44362, CEDEX 4, 31030 Toulouse, France
| | - Gabriel Furtos
- Department of Dental Materials, Institute of Research in Chemistry, Babes-Bolyai University-Raluca Ripan, Fantanele Street 30, 400294 Cluj-Napoca, Romania
| | - Véronique Fontaine
- Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium
| |
Collapse
|
|
10
|
Zahid A, Yu Y, Zhou S, Xie X, Yin F. Antiparasitic effect of copper alloy mesh on tomont stage of Cryptocaryon irritans in aquaculture. JOURNAL OF FISH DISEASES 2023; 46:181-188. [PMID: 36453691 DOI: 10.1111/jfd.13732] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/10/2022] [Revised: 11/09/2022] [Accepted: 11/10/2022] [Indexed: 06/17/2023]
Abstract
Copper alloy sheets have been shown to prevent cryptocaryoniasis. Therefore, we studied the potential efficiency of copper alloy mesh (CAM) in aquaculture tanks to prevent cryptocaryoniasis outbreaks. The effectivenesses of CAM against the tomont stage of Cryptocaryon irritans and in protecting fish from cryptocaryoniasis were tested both in vitro and in vivo. The mortality rate of C. irritans tomonts increased as the contact time with CAM rose and peaked at 70 min (100% of mortality). Morphological changes were observed such as the shrinking of the protoplasm of the treated tomonts, resulting in a larger gap between the cytoplasm and the cyst wall. Mitochondrial dysfunction due to shrinkage in the inner portion, outer and inner mitochondrial membrane damage and cytoplasmic vacuolation was revealed by ultrastructural analysis. The use of CAM effectively preventing reinfection was also provided. In comparison with group B (infected fish without CAM), both groups A (uninfected fish as a control group) and C (infected fish treated with CAM) had a 100% survival rate until the end of the trial. CAM has the same anticryptocaryoniasis effect as copper alloy sheets but is more advantageous due to its lightweight, reduced labor cost and lower purchase cost. It is noticeable that CAM exposure also prevents the excessive accumulation of copper ions in aquaculture sea water.
Collapse
Affiliation(s)
- Aysha Zahid
- Key Laboratory of Applied Marine Biotechnology, Ministry of Education; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products; School of Marine Sciences, Ningbo University, Ningbo, China
| | - Youbin Yu
- Key Laboratory of Ocean Fishing Vessel and Equipment, Ministry of Agriculture and Rural Affairs, Fishery Machinery and Instrument Research institute, Chinese Academy of Fishery Sciences, Shanghai, China
| | - Suming Zhou
- Key Laboratory of Applied Marine Biotechnology, Ministry of Education; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products; School of Marine Sciences, Ningbo University, Ningbo, China
| | - Xiao Xie
- Key Laboratory of Applied Marine Biotechnology, Ministry of Education; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products; School of Marine Sciences, Ningbo University, Ningbo, China
| | - Fei Yin
- Key Laboratory of Applied Marine Biotechnology, Ministry of Education; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products; School of Marine Sciences, Ningbo University, Ningbo, China
| |
Collapse
|
|
11
|
Timoncini A, Costantini F, Bernardi E, Martini C, Mugnai F, Mancuso FP, Sassoni E, Ospitali F, Chiavari C. Insight on bacteria communities in outdoor bronze and marble artefacts in a changing environment. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 850:157804. [PMID: 35932861 DOI: 10.1016/j.scitotenv.2022.157804] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/17/2022] [Revised: 07/12/2022] [Accepted: 07/30/2022] [Indexed: 06/15/2023]
Abstract
Epilithic bacteria play a fundamental role in the conservation of cultural heritage (CH) materials. On stones, bacterial communities cause both degradation and bioprotection actions. Bronze biocorrosion in non-burial conditions is rarely studied. Only few studies have examined the relationship between bacteria communities and the chemical composition of patinas (surface degradation layers). A better comprehension of bacterial communities growing on our CH is fundamental not only to understand the related decay mechanisms but also to foresee possible shifts in their composition due to climate change. The present study aims at (1) characterizing bacterial communities on bronze and marble statues; (2) evaluating the differences in bacterial communities' composition and abundance occurring between different patina types on different statues; and (3) providing indications about a representative bacterial community which can be used in laboratory tests to better understand their influence on artefact decay. Chemical and biological characterization of different patinas were carried out by sampling bronze and marble statues in Bologna and Ravenna (Italy), using EDS/Raman spectroscopy and MinION-based 16SrRNA sequencing. Significant statistical differences were found in bacterial composition between marble and bronze statues, and among marble patinas in different statues and in the same statue. Marble surfaces showed high microbial diversity and were characterized mainly by Cyanobacteria, Proteobacteria and Deinococcus-Thermus. Bronze patinas showed low taxa diversity and were dominated by copper-resistant Proteobacteria. The copper biocidal effect is evident in greenish marble areas affected by the leaching of copper salts, where the bacterial community is absent. Here, Ca and Cu oxalates are present because of the biological reaction of living organisms to Cu ions, leading to metabolic product secretions, such as oxalic acid. Therefore, a better knowledge on the interaction between bacteria communities and patinas has been achieved.
Collapse
Affiliation(s)
- Andrea Timoncini
- Department of Cultural Heritage, University of Bologna, Via degli Ariani 1, 48121 Ravenna, Italy
| | - Federica Costantini
- Department of Biological, Geological and Environmental Science, UOS Ravenna, University of Bologna, Via Sant'Alberto 163, 48123 Ravenna, Italy; Interdepartmental Center for Industrial Research Renewable Sources, Environment, Sea and Energy, University of Bologna, Ravenna, Italy; Interdepartmental Research Center for Environmental Sciences, University of Bologna, Ravenna, Italy
| | - Elena Bernardi
- Department of Industrial Chemistry "Toso Montanari", University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy
| | - Carla Martini
- Department of Industrial Engineering, University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy
| | - Francesco Mugnai
- Department of Biological, Geological and Environmental Science, UOS Ravenna, University of Bologna, Via Sant'Alberto 163, 48123 Ravenna, Italy
| | - Francesco Paolo Mancuso
- Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze Ed. 16, 90128 Palermo, Italy
| | - Enrico Sassoni
- Department Of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, Italy
| | - Francesca Ospitali
- Department of Industrial Chemistry "Toso Montanari", University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy
| | - Cristina Chiavari
- Department of Cultural Heritage, University of Bologna, Via degli Ariani 1, 48121 Ravenna, Italy.
| |
Collapse
|
|
12
|
Dhayanithi NB, Sudhagar A, Kumar TTA, Lal KK. Study on amyloodiniosis outbreak in captive-bred percula clownfish ( Amphiprion percula) and improved control regimens. J Parasit Dis 2022; 46:1103-1109. [PMID: 36457777 PMCID: PMC9606147 DOI: 10.1007/s12639-022-01530-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/27/2022] [Accepted: 08/30/2022] [Indexed: 10/14/2022] Open
Abstract
The amyloodiniosis outbreak was documented with high mortality of percula clownfish (Amphiprion percula) and subsequently, various therapeutics were evaluated to control the infection. The affected fish exhibited symptoms such as discoloration of the skin and jerky movement with severe respiratory stress. Microscopic examination of the gill and body surface of the infested moribund fish showed the presence of brown coloured spherical shaped trophonts with the size range of 150-300 µM. Copper sulphate (10 ppm) and hydroxychloroquine phosphate (10 ppm) treatment with 14 days of continuous bath showed 76 ± 5.3 and 66 ± 4.3% survival rates. However, formalin (10 ppm) and malachite green (10 ppm) treated groups showed a survival of 41 ± 1.7 and 32.7 ± 1.2% respectively. The present results suggest that, use of copper sulphate to treat amyloodiniosis in clownfish will relatively more effective than other treatment options. The findings will be helpful to mitigate amyloodiniosis in marine finfish aquaculture, particularly marine ornamentals.
Collapse
Affiliation(s)
| | - Arun Sudhagar
- ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh 226002 India
| | | | - Kuldeep Kumar Lal
- ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh 226002 India
| |
Collapse
|
|
13
|
Dhyani A, Repetto T, Bartikofsky D, Mirabelli C, Gao Z, Snyder SA, Snyder C, Mehta G, Wobus CE, VanEpps JS, Tuteja A. Surfaces with instant and persistent antimicrobial efficacy against bacteria and SARS-CoV-2. MATTER 2022; 5:4076-4091. [PMID: 36034972 PMCID: PMC9399129 DOI: 10.1016/j.matt.2022.08.018] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 03/08/2022] [Revised: 07/26/2022] [Accepted: 08/16/2022] [Indexed: 06/15/2023]
Abstract
Surfaces contaminated with bacteria and viruses contribute to the transmission of infectious diseases and pose a significant threat to global public health. Modern day disinfection either relies on fast-acting (>3-log reduction within a few minutes), yet impermanent, liquid-, vapor-, or radiation-based disinfection techniques, or long-lasting, but slower-acting, passive antimicrobial surfaces based on heavy metal surfaces, or metallic nanoparticles. There is currently no surface that provides instant and persistent antimicrobial efficacy against a broad spectrum of bacteria and viruses. In this work, we describe a class of extremely durable antimicrobial surfaces incorporating different plant secondary metabolites that are capable of rapid disinfection (>4-log reduction) of current and emerging pathogens within minutes, while maintaining persistent efficacy over several months and under significant environmental duress. We also show that these surfaces can be readily applied onto a variety of desired substrates or devices via simple application techniques such as spray, flow, or brush coating.
Collapse
Affiliation(s)
- Abhishek Dhyani
- Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
| | - Taylor Repetto
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
| | - Dylan Bartikofsky
- Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
| | - Carmen Mirabelli
- Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
| | - Zhihe Gao
- Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
| | - Sarah A Snyder
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
| | - Catherine Snyder
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
| | - Geeta Mehta
- Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
| | - Christiane E Wobus
- Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA
| | - J Scott VanEpps
- Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
- Departments of Emergency Medicine, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Michigan Center for Integrative Research in Critical Care, University of Michigan, Ann Arbor, MI 48109, USA
| | - Anish Tuteja
- Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA
- Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
| |
Collapse
|
|
14
|
Razavipour M, Gonzalez M, Singh N, Cimenci CE, Chu N, Alarcon EI, Villafuerte J, Jodoin B. Biofilm Inhibition and Antiviral Response of Cold Sprayed and Shot Peened Copper Surfaces: Effect of Surface Morphology and Microstructure. JOURNAL OF THERMAL SPRAY TECHNOLOGY 2022; 31:130-144. [PMID: 37520908 PMCID: PMC8735887 DOI: 10.1007/s11666-021-01315-7] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/01/2021] [Revised: 12/14/2021] [Accepted: 12/19/2021] [Indexed: 08/01/2023]
Abstract
Antibacterial properties of copper against planktonic bacteria population are affected by surface microstructure and topography. However, copper interactions with bacteria in a biofilm state are less studied. This work aims at better understanding the difference in biofilm inhibition of bulk, cold-sprayed, and shot-peened copper surfaces and gaining further insights on the underlying mechanisms using optical and scanning electron microscopy to investigate the topography and microstructure of the surfaces. The biofilm inhibition ability is reported for all surfaces. Results show that the biofilm inhibition performance of cold sprayed copper, while initially better, decreases with time and results in an almost identical performance than as-received copper after 18h incubation time. The shot-peened samples with a rough and ultrafine microstructure demonstrated an enhanced biofilm control, especially at 18 hr. The biofilm control mechanisms were explained by the diffusion rates and concentration of copper ions and the interaction between these ions and the biofilm, while surface topography plays a role in the bacteria attachment at the early planktonic state. Furthermore, the data suggest that surface topography plays a key role in antiviral activity of the materials tested, with a smooth surface being the most efficient. Graphical Abstract
Collapse
Affiliation(s)
- Maryam Razavipour
- Cold Spray Research Laboratory, University of Ottawa, Ottawa, ON Canada
| | - Mayte Gonzalez
- Division of Cardiac Surgery, BEaTS Research, University of Ottawa Heart Institute, Ottawa, Ontario Canada
| | - Naveen Singh
- Cold Spray Research Laboratory, University of Ottawa, Ottawa, ON Canada
| | - Cagla Eren Cimenci
- Division of Cardiac Surgery, BEaTS Research, University of Ottawa Heart Institute, Ottawa, Ontario Canada
| | - Nicole Chu
- Division of Cardiac Surgery, BEaTS Research, University of Ottawa Heart Institute, Ottawa, Ontario Canada
| | - Emilio I. Alarcon
- Division of Cardiac Surgery, BEaTS Research, University of Ottawa Heart Institute, Ottawa, Ontario Canada
- Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario Canada
| | | | - Bertrand Jodoin
- Cold Spray Research Laboratory, University of Ottawa, Ottawa, ON Canada
| |
Collapse
|
|
15
|
Ermakov VV, Jovanović LN. Biological Role of Trace Elements and Viral Pathologies. GEOCHEMISTRY INTERNATIONAL 2022; 60. [PMCID: PMC8853261 DOI: 10.1134/s0016702922020045] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/03/2023]
Abstract
The review presents summarized information on a new research avenue in biogeochemistry and geochemical ecology: relationships between the microcosm (viruses) and manifestations of animal and human pathologies. Some aspects of the biological action of selenium, zinc, copper and iodine, their influence on the manifestation and course of viral infections are considered. Attention is focused on the antioxidant, membrane-protective, boosting immunity, hormonal functions of trace elements, and on the antibacterial and antiviral properties of metallic copper and its compounds. The criteria currently applied in assessing the Se status of territories are compared with the incidence of COVID-19 and HIV in the population of the Russian Federation. In some cases, selenium deficiency in the environment is shown to be associated with a higher susceptibility to RNA viral infections. Emphasis is put on the necessity of improving the criteria for assessing the trace element status of territories and further studies in the geochemical ecology of viruses and their role in the biosphere.
Collapse
Affiliation(s)
- V. V. Ermakov
- Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
| | - L. N. Jovanović
- ALFA BK University, Palmira Toljatija, 3, 11070 Belgrade, Serbia
| |
Collapse
|
|
16
|
Manakhov AM, Sitnikova NA, Tsygankova AR, Alekseev AY, Adamenko LS, Permyakova E, Baidyshev VS, Popov ZI, Blahová L, Eliáš M, Zajíčková L, Solovieva AO. Electrospun Biodegradable Nanofibers Coated Homogenously by Cu Magnetron Sputtering Exhibit Fast Ion Release. Computational and Experimental Study. MEMBRANES 2021; 11:965. [PMID: 34940466 PMCID: PMC8708309 DOI: 10.3390/membranes11120965] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/17/2021] [Revised: 12/03/2021] [Accepted: 12/04/2021] [Indexed: 11/30/2022]
Abstract
Copper-coated nanofibrous materials are desirable for catalysis, electrochemistry, sensing, and biomedical use. The preparation of copper or copper-coated nanofibers can be pretty challenging, requiring many chemical steps that we eliminated in our robust approach, where for the first time, Cu was deposited by magnetron sputtering onto temperature-sensitive polymer nanofibers. For the first time, the large-scale modeling of PCL films irradiation by molecular dynamics simulation was performed and allowed to predict the ions penetration depth and tune the deposition conditions. The Cu-coated polycaprolactone (PCL) nanofibers were thoroughly characterized and tested as antibacterial agents for various Gram-positive and Gram-negative bacteria. Fast release of Cu2+ ions (concentration up to 3.4 µg/mL) led to significant suppression of E. coli and S. aureus colonies but was insufficient against S. typhimurium and Ps. aeruginosa. The effect of Cu layer oxidation upon contact with liquid media was investigated by X-ray photoelectron spectroscopy revealing that, after two hours, 55% of Cu atoms are in form of CuO or Cu(OH)2. The Cu-coated nanofibers will be great candidates for wound dressings thanks to an interesting synergistic effect: on the one hand, the rapid release of copper ions kills bacteria, while on the other hand, it stimulates the regeneration with the activation of immune cells. Indeed, copper ions are necessary for the bacteriostatic action of cells of the immune system. The reactive CO2/C2H4 plasma polymers deposited onto PCL-Cu nanofibers can be applied to grafting of viable proteins, peptides, or drugs, and it further explores the versatility of developed nanofibers for biomedical applications use.
Collapse
Affiliation(s)
- Anton M. Manakhov
- Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova St., 630060 Novosibirsk, Russia; (N.A.S.); (E.P.)
| | - Natalya A. Sitnikova
- Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova St., 630060 Novosibirsk, Russia; (N.A.S.); (E.P.)
| | - Alphiya R. Tsygankova
- Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russia;
| | - Alexander Yu. Alekseev
- Research Institute of Virology, The Federal Research Center of Fundamental and Translational Medicine, 2 Timakova St., 630060 Novosibirsk, Russia; (A.Y.A.); (L.S.A.)
- Research Institute of Applied Ecology, Dagestan State University, Dahadaeva 21, 367000 Makhachkala, Russia
| | - Lyubov S. Adamenko
- Research Institute of Virology, The Federal Research Center of Fundamental and Translational Medicine, 2 Timakova St., 630060 Novosibirsk, Russia; (A.Y.A.); (L.S.A.)
| | - Elizaveta Permyakova
- Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova St., 630060 Novosibirsk, Russia; (N.A.S.); (E.P.)
- Laboratory of Inorganic Nanomaterials, National University of Science and Technology “MISiS”, Leninsky Prospekt 4, 119071 Moscow, Russia
| | - Victor S. Baidyshev
- Department of Computer Engineering and Automated Systems Software, Katanov Khakas State University, Pr. Lenin, 90, 655017 Abakan, Russia;
| | - Zakhar I. Popov
- Laboratory of Acoustic Microscopy, Emanuel Institute of Biochemical Physics RAS, Kosygina 4, 119334 Moscow, Russia;
| | - Lucie Blahová
- Central European Institute of Technology CEITEC-BUT, Purkyňova 123, 61200 Brno, Czech Republic; (L.B.); (M.E.); (L.Z.)
| | - Marek Eliáš
- Central European Institute of Technology CEITEC-BUT, Purkyňova 123, 61200 Brno, Czech Republic; (L.B.); (M.E.); (L.Z.)
| | - Lenka Zajíčková
- Central European Institute of Technology CEITEC-BUT, Purkyňova 123, 61200 Brno, Czech Republic; (L.B.); (M.E.); (L.Z.)
- Department Condensed Matter Physics, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic
| | - Anastasiya O. Solovieva
- Research Institute of Clinical and Experimental Lymphology—Branch of the ICG SB RAS, 2 Timakova St., 630060 Novosibirsk, Russia; (N.A.S.); (E.P.)
| |
Collapse
|
|
17
|
Smith JL, Tran N, Song T, Liang D, Qian M. Robust bulk micro-nano hierarchical copper structures possessing exceptional bactericidal efficacy. Biomaterials 2021; 280:121271. [PMID: 34864450 DOI: 10.1016/j.biomaterials.2021.121271] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/24/2020] [Revised: 11/01/2021] [Accepted: 11/22/2021] [Indexed: 12/29/2022]
Abstract
Conventional copper (Cu) metal surfaces are well recognized for their bactericidal properties. However, their slow bacteria-killing potency has historically excluded them as a rapid bactericidal material. We report the development of a robust bulk superhydrophilic micro-nano hierarchical Cu structure that possesses exceptional bactericidal efficacy. It resulted in a 4.41 log10 reduction (>99.99%) of the deadly Staphylococcus aureus (S. aureus) bacteria within 2 min vs. a 1.49 log10 reduction (96.75%) after 240 min on common Cu surfaces. The adhered cells exhibited extensive blebbing, loss of structural integrity and leakage of vital intracellular material, demonstrating the rapid efficacy of the micro-nano Cu structure in destructing bacteria membrane integrity. The mechanism was attributed to the synergistic degradation of the cell envelope through enhanced release and therefore uptake of the cytotoxic Cu ions and the adhesion-driven mechanical strain due to its rapid ultimate superhydrophilicity (contact angle drops to 0° in 0.18 s). The scalable fabrication of this micro-nano Cu structure was enabled by integrating bespoke precursor alloy design with microstructure preconditioning for dealloying and demonstrated on 2000 mm2 Cu surfaces. This development paves the way to the practical exploitation of Cu as a low-cost antibiotic-free fast bactericidal material.
Collapse
Affiliation(s)
- J L Smith
- RMIT University, School of Engineering, Melbourne, Victoria, 3000, Australia; CSIRO, Manufacturing, Clayton, Victoria, 3168, Australia
| | - N Tran
- RMIT University, School of Science, Melbourne, Victoria, 3000, Australia
| | - T Song
- RMIT University, School of Engineering, Melbourne, Victoria, 3000, Australia
| | - D Liang
- CSIRO, Manufacturing, Clayton, Victoria, 3168, Australia
| | - M Qian
- RMIT University, School of Engineering, Melbourne, Victoria, 3000, Australia.
| |
Collapse
|
|
18
|
Antibacterial Efficacy of Cold-Sprayed Copper Coatings against Gram-Positive Staphylococcus aureus and Gram-Negative Escherichia coli. MATERIALS 2021; 14:ma14226744. [PMID: 34832144 PMCID: PMC8626024 DOI: 10.3390/ma14226744] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/16/2021] [Revised: 10/31/2021] [Accepted: 11/04/2021] [Indexed: 11/28/2022]
Abstract
Contact surfaces have been identified as one of the main routes for pathogen transmission. The efficacy to kill both viruses and bacteria on touch surfaces is critical to reducing the rampant spread of harmful pathogens. Copper is one such material that has been traditionally used for its antimicrobial properties. However, most contact/touch surfaces are made up of steel or aluminum due to their structural properties. Therefore, coating high-touch components with copper is one possible solution to improve antibacterial efficacy. In this study, copper was coated on both stainless steel and aluminum substrates using a cold spray process which is a fast and economic coating technique. The coated samples in both as-deposited and heat-treated states were exposed to Escherichia coli and Staphylococcus aureus bacteria, and their efficacy was compared with bulk copper plate. It was found that both bacterial cells responded differently to the different coating properties such as coating thickness, porosity, hardness, surface roughness, oxide content, and galvanic coupling effect. These correlations were elucidated in light of various results obtained from antibacterial and bacterial attachment tests, and materials characterizations of the coatings. It is possible to tailor copper coating characteristics to render them more effective against targeted bacteria.
Collapse
|
|
19
|
Bäumler W, Eckl D, Holzmann T, Schneider-Brachert W. Antimicrobial coatings for environmental surfaces in hospitals: a potential new pillar for prevention strategies in hygiene. Crit Rev Microbiol 2021; 48:531-564. [PMID: 34699296 DOI: 10.1080/1040841x.2021.1991271] [Citation(s) in RCA: 21] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Abstract
Recent reports provide evidence that contaminated healthcare environments represent major sources for the acquisition and transmission of pathogens. Antimicrobial coatings (AMC) may permanently and autonomously reduce the contamination of such environmental surfaces complementing standard hygiene procedures. This review provides an overview of the current status of AMC and the demands to enable a rational application of AMC in health care settings. Firstly, a suitable laboratory test norm is required that adequately quantifies the efficacy of AMC. In particular, the frequently used wet testing (e.g. ISO 22196) must be replaced by testing under realistic, dry surface conditions. Secondly, field studies should be mandatory to provide evidence for antimicrobial efficacy under real-life conditions. The antimicrobial efficacy should be correlated to the rate of nosocomial transmission at least. Thirdly, the respective AMC technology should not add additional bacterial resistance development induced by the biocidal agents and co- or cross-resistance with antibiotic substances. Lastly, the biocidal substances used in AMC should be safe for humans and the environment. These measures should help to achieve a broader acceptance for AMC in healthcare settings and beyond. Technologies like the photodynamic approach already fulfil most of these AMC requirements.
Collapse
Affiliation(s)
- Wolfgang Bäumler
- Department of Dermatology, University Hospital, Regensburg, Germany
| | - Daniel Eckl
- Department of Microbiology, University of Regensburg, Regensburg, Germany
| | - Thomas Holzmann
- Department of Infection Control and Infectious Diseases, University Hospital, Regensburg, Germany
| | - Wulf Schneider-Brachert
- Department of Infection Control and Infectious Diseases, University Hospital, Regensburg, Germany
| |
Collapse
|
|
20
|
Zhang Y, Yang S, Wang J, Cai Y, Niu L, Liu X, Liu C, Qi H, Liu A. Copper sulfide nanoclusters with multi-enzyme-like activities and its application in acid phosphatase sensing based on enzymatic cascade reaction. Talanta 2021; 233:122594. [PMID: 34215083 DOI: 10.1016/j.talanta.2021.122594] [Citation(s) in RCA: 31] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/24/2021] [Revised: 06/03/2021] [Accepted: 06/05/2021] [Indexed: 12/16/2022]
Abstract
Nanozymes are artificial enzymes, which can substitute natural enzymes for wide range of catalysis-based applications. However, it is challenging to explore novel mimic enzymes or multi-enzyme mimics. Herein we report the facile preparation of uniform CuS nanoclusters (NCs), which possessed outstanding tetra-enzyme mimetic catalytic activities, including peroxidase (POD)-mimics, catalase (CAT)-mimics, ascorbic acid oxidase (AAO)-mimics and superoxide dismutase (SOD)-mimics. The catalytic mechanism of POD-like was coming from the oxygen vacancies of CuS. Furthermore, the steady-state kinetics of POD-, CAT- and AAO mimics of CuS NCs were systematically explored. On basis of the enzymatic cascade reaction that acid phosphatase (ACP) involved in a weak acidic environment, in the presence of O-phenylenediamine, quinoxaline fluorescent substance will be generated. Thus, a fluorescent biosensor platform was proposed for detection of ACP with the linear range of 0.05-25 U L-1 and limit of detection of 0.01 U L-1. The as-proposed method was applicable to real serum sample detection accurately and reproducibly. Considering the simple preparation, good stability, excellent multi-enzyme activities and controllable experimental operation, CuS NCs would provide a basis for expanding to other biocatalytic and biomedical applications.
Collapse
Affiliation(s)
- Yujiao Zhang
- Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Shuqing Yang
- Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Jin Wang
- Qingdao Institute for Food and Drug Control, 7 Longde Road, Qingdao, 266073, China
| | - Yuanyuan Cai
- School of Pharmacy, Medical College, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Lingxi Niu
- Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Xuan Liu
- Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Chongyang Liu
- School of Pharmacy, Medical College, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China
| | - Huan Qi
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200240, China.
| | - Aihua Liu
- Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China; School of Pharmacy, Medical College, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China.
| |
Collapse
|
|
21
|
Walji SD, Bruder MR, Aucoin MG. Virus matrix interference on assessment of virucidal activity of high-touch surfaces designed to prevent hospital-acquired infections. Antimicrob Resist Infect Control 2021; 10:133. [PMID: 34507617 PMCID: PMC8431935 DOI: 10.1186/s13756-021-01001-x] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/12/2020] [Accepted: 08/27/2021] [Indexed: 12/24/2022] Open
Abstract
Objectives/purpose High-touch surfaces are a critical reservoir in the spread of nosocomial infections. Although disinfection and infection control protocols are well developed, they lack the ability to passively reduce the pathogenic load of high-touch surfaces. Copper and its alloys have been suggested as a surface that exhibit continuous biocidal effects. Antimicrobial studies on these surfaces are prevalent, while virucidal studies are not as well explored. The goal of this study was to first determine the virucidal activity of a copper–nickel–zinc alloy and to then examine the effect of soiling and virus preparation on virucidal activity. Methods A baculovirus vector was used as an easily quantifiable model of an infectious enveloped animal cell virus. Droplets containing virus were deposited on surfaces and allowed to stay wet using humidity control or were dried onto the surface. Virus was then recovered from the surface and assayed for infectivity. To examine how the composition of the droplet affected the survival of the virus, 3 different soiling conditions were tested. The first two were recommended by the United States Environmental Protection Agency and the third consisted of cell debris resulting from virus amplification. Results A copper–nickel–zinc alloy was shown to have strong virucidal effects for an enveloped virus. Copper, nickel, and zinc ions were all shown to leach from the alloy surface and are the likely cause of virucidal activity by this surface. Virucidal activity was achieved under moderate soiling but lost under high soiling generated by routine virus amplification procedures. The surface was able to repeatably inactivate dried virus droplets under moderate soiling conditions, but unable to do so for virus droplets kept wet using high humidity. Conclusion Ion leaching was associated with virucidal activity in both wet and dried virus conditions. Soiling protected the virus by quenching metal ions, and not by inhibiting leaching. The composition of the solution containing virus plays a critical role in evaluating the virucidal activity of surfaces and surface coatings.
Collapse
Affiliation(s)
- Sadru-Dean Walji
- Department of Chemical Engineering, University of Waterloo, 200 University Ave West, Waterloo, ON, N2L 3G1, Canada
| | - Mark R Bruder
- Department of Chemical Engineering, University of Waterloo, 200 University Ave West, Waterloo, ON, N2L 3G1, Canada
| | - Marc G Aucoin
- Department of Chemical Engineering, University of Waterloo, 200 University Ave West, Waterloo, ON, N2L 3G1, Canada.
| |
Collapse
|
|
22
|
Seifi T, Reza Kamali A. Antiviral performance of graphene-based materials with emphasis on COVID-19: A review. MEDICINE IN DRUG DISCOVERY 2021; 11:100099. [PMID: 34056572 PMCID: PMC8151376 DOI: 10.1016/j.medidd.2021.100099] [Citation(s) in RCA: 33] [Impact Index Per Article: 8.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2021] [Revised: 05/06/2021] [Accepted: 05/19/2021] [Indexed: 02/06/2023] Open
Abstract
Coronavirus disease-2019 has been one of the most challenging global epidemics of modern times with a large number of casualties combined with economic hardships across the world. Considering that there is still no definitive cure for the recent viral crisis, this article provides a review of nanomaterials with antiviral activity, with an emphasis on graphene and its derivatives, including graphene oxide, reduced graphene oxide and graphene quantum dots. The possible interactions between surfaces of such nanostructured materials with coronaviruses are discussed. The antiviral mechanisms of graphene materials can be related to events such as the inactivation of virus and/or the host cell receptor, electrostatic trapping and physico-chemical destruction of viral species. These effects can be enhanced by functionalization and/or decoration of carbons with species that enhances graphene-virus interactions. The low-cost and large-scale preparation of graphene materials with enhanced antiviral performances is an interesting research direction to be explored.
Collapse
Affiliation(s)
- Tahereh Seifi
- Energy and Environmental Materials Research Centre (E2MC), School of Metallurgy, Northeastern University, Shenyang 110819, China
| | - Ali Reza Kamali
- Energy and Environmental Materials Research Centre (E2MC), School of Metallurgy, Northeastern University, Shenyang 110819, China
| |
Collapse
|
|
23
|
Abraham J, Dowling K, Florentine S. Can Copper Products and Surfaces Reduce the Spread of Infectious Microorganisms and Hospital-Acquired Infections? MATERIALS (BASEL, SWITZERLAND) 2021; 14:3444. [PMID: 34206230 PMCID: PMC8269470 DOI: 10.3390/ma14133444] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/26/2021] [Revised: 06/17/2021] [Accepted: 06/19/2021] [Indexed: 01/04/2023]
Abstract
Pathogen transfer and infection in the built environment are globally significant events, leading to the spread of disease and an increase in subsequent morbidity and mortality rates. There are numerous strategies followed in healthcare facilities to minimize pathogen transfer, but complete infection control has not, as yet, been achieved. However, based on traditional use in many cultures, the introduction of copper products and surfaces to significantly and positively retard pathogen transmission invites further investigation. For example, many microbes are rendered unviable upon contact exposure to copper or copper alloys, either immediately or within a short time. In addition, many disease-causing bacteria such as E. coli O157:H7, hospital superbugs, and several viruses (including SARS-CoV-2) are also susceptible to exposure to copper surfaces. It is thus suggested that replacing common touch surfaces in healthcare facilities, food industries, and public places (including public transport) with copper or alloys of copper may substantially contribute to limiting transmission. Subsequent hospital admissions and mortality rates will consequently be lowered, with a concomitant saving of lives and considerable levels of resources. This consideration is very significant in times of the COVID-19 pandemic and the upcoming epidemics, as it is becoming clear that all forms of possible infection control measures should be practiced in order to protect community well-being and promote healthy outcomes.
Collapse
Affiliation(s)
- Joji Abraham
- School of Engineering, Information Technology and Physical Sciences, Mt Helen Campus, Ballarat, VIC 3353, Australia;
| | - Kim Dowling
- School of Engineering, Information Technology and Physical Sciences, Mt Helen Campus, Ballarat, VIC 3353, Australia;
- Department of Geology, University of Johannesburg, Johannesburg 2006, South Africa
| | - Singarayer Florentine
- Future Regions Research Centre, School of Science, Psychology and Sport, Federation University Australia, Mt Helen Campus, Ballarat, VIC 3353, Australia;
| |
Collapse
|
|
24
|
Caro-Lara L, Ramos-Moore E, Vargas IT, Walczak M, Fuentes C, Gómez AV, Barrera NP, Castillo J, Pizarro G. Initial adhesion suppression of biofilm-forming and copper-tolerant bacterium Variovorax sp. on laser microtextured copper surfaces. Colloids Surf B Biointerfaces 2021; 202:111656. [PMID: 33735634 DOI: 10.1016/j.colsurfb.2021.111656] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/24/2020] [Revised: 02/13/2021] [Accepted: 02/22/2021] [Indexed: 11/24/2022]
Abstract
The growth of detrimental biofilms on metal surfaces affects their structural performance and lifespan. Microtopographic texturization has emerged as an approach to suppress biofilm growth by preventing the initial stages of bacterial adhesion. This work studies the effects of linear pattern copper texturization on the initial adhesion steps of the biofilm-forming and copper-resistant bacterium Variovorax sp. Linear patterns with 4.7, 6.8, 14, and 18 μm periodicity were produced by direct laser interference patterning (DLIP) on copper coupons. Surface features were characterized by microscopic and spectroscopic techniques, and bacterial adhesion behavior was characterized by epifluorescence microscopy and functionalization of atomic force microscopy tips. We found a periodicity of 4.7 μm as the most efficient pattern to suppress Variovorax sp. initial adhesion by 31.1 % with respect to the nontextured surface. Preferential settlement in hummocks over hollows was observed for patterns with 14 and 18 μm periodicity, with adhesion events showing higher frequency in these topographies than patterns with periodicities of 4.7 and 6.8 μm. Our results highlight an understanding of the initial bacteria-copper adhesion and settlement behavior, thus contributing to the potential development of innocuous strategies for controlling biofilm growth on copper-based materials.
Collapse
Affiliation(s)
- Luis Caro-Lara
- Departamento de Ingeniería Hidráulica y Ambiental, Pontificia Universidad Católica de Chile, Santiago, Chile; Marine Energy Research & Innovation Center. Chile.
| | - Esteban Ramos-Moore
- Instituto de Física, Facultad de Física, Pontificia Universidad Católica de Chile Casilla 306, Av. Vicuña Mackenna 4860, Macul, Santiago, Chile; Centro de Investigación en Nanotecnología y Materiales Avanzados (CIEN-UC), Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Macul, Santiago, Chile.
| | - Ignacio T Vargas
- Departamento de Ingeniería Hidráulica y Ambiental, Pontificia Universidad Católica de Chile, Santiago, Chile; Marine Energy Research & Innovation Center. Chile
| | - Magdalena Walczak
- Marine Energy Research & Innovation Center. Chile; Departamento de Ingeniería Mecánica y Metalúrgica, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Macul, Santiago, Chile
| | - Christian Fuentes
- Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile
| | - Andrea V Gómez
- Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile; Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile
| | - Nelson P Barrera
- Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile
| | - Javiera Castillo
- Departamento de Ingeniería Hidráulica y Ambiental, Pontificia Universidad Católica de Chile, Santiago, Chile
| | - Gonzalo Pizarro
- Departamento de Ingeniería Hidráulica y Ambiental, Pontificia Universidad Católica de Chile, Santiago, Chile; Marine Energy Research & Innovation Center. Chile
| |
Collapse
|
|
25
|
Dauvergne E, Mullié C. Brass Alloys: Copper-Bottomed Solutions against Hospital-Acquired Infections? Antibiotics (Basel) 2021; 10:antibiotics10030286. [PMID: 33801855 PMCID: PMC7999369 DOI: 10.3390/antibiotics10030286] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/03/2021] [Revised: 02/26/2021] [Accepted: 03/08/2021] [Indexed: 12/26/2022] Open
Abstract
Copper has been used for its antimicrobial properties since Antiquity. Nowadays, touch surfaces made of copper-based alloys such as brasses are used in healthcare settings in an attempt to reduce the bioburden and limit environmental transmission of nosocomial pathogens. After a brief history of brass uses, the various mechanisms that are thought to be at the basis of brass antimicrobial action will be described. Evidence shows that direct contact with the surface as well as cupric and cuprous ions arising from brass surfaces are instrumental in the antimicrobial effectiveness. These copper ions can lead to oxidative stress, membrane alterations, protein malfunctions, and/or DNA damages. Laboratory studies back up a broad spectrum of activity of brass surfaces on bacteria with the possible exception of bacteria in their sporulated form. Various parameters influencing the antimicrobial activity such as relative humidity, temperature, wet/dry inoculation or wear have been identified, making it mandatory to standardize antibacterial testing. Field trials using brass and copper surfaces consistently report reductions in the bacterial bioburden but, evidence is still sparse as to a significant impact on hospital acquired infections. Further work is also needed to assess the long-term effects of chemical/physical wear on their antimicrobial effectiveness.
Collapse
Affiliation(s)
- Emilie Dauvergne
- Laboratoire AGIR-UR UPJV 4294, UFR de Pharmacie, Université de Picardie Jules Verne, 80037 Amiens, France;
- FAVI Limited Company, 80490 Hallencourt, France
| | - Catherine Mullié
- Laboratoire AGIR-UR UPJV 4294, UFR de Pharmacie, Université de Picardie Jules Verne, 80037 Amiens, France;
- Laboratoire Hygiène, Risque Biologique et Environnement, Centre Hospitalier Universitaire Amiens-Picardie, 80025 Amiens, France
- Correspondence:
| |
Collapse
|
|
26
|
Chang T, Sepati M, Herting G, Leygraf C, Rajarao GK, Butina K, Richter-Dahlfors A, Blomberg E, Odnevall Wallinder I. A novel methodology to study antimicrobial properties of high-touch surfaces used for indoor hygiene applications-A study on Cu metal. PLoS One 2021; 16:e0247081. [PMID: 33630868 PMCID: PMC7906481 DOI: 10.1371/journal.pone.0247081] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/10/2020] [Accepted: 02/01/2021] [Indexed: 01/30/2023] Open
Abstract
Metal-based high-touch surfaces used for indoor applications such as doorknobs, light switches, handles and desks need to remain their antimicrobial properties even when tarnished or degraded. A novel laboratory methodology of relevance for indoor atmospheric conditions and fingerprint contact has therefore been elaborated for combined studies of both tarnishing/corrosion and antimicrobial properties of such high-touch surfaces. Cu metal was used as a benchmark material. The protocol includes pre-tarnishing/corrosion of the high touch surface for different time periods in a climatic chamber at repeated dry/wet conditions and artificial sweat deposition followed by the introduction of bacteria onto the surfaces via artificial sweat droplets. This methodology provides a more realistic and reproducible approach compared with other reported procedures to determine the antimicrobial efficiency of high-touch surfaces. It provides further a possibility to link the antimicrobial characteristics to physical and chemical properties such as surface composition, chemical reactivity, tarnishing/corrosion, surface roughness and surface wettability. The results elucidate that bacteria interactions as well as differences in extent of tarnishing can alter the physical properties (e.g. surface wettability, surface roughness) as well as the extent of metal release. The results clearly elucidate the importance to consider changes in chemical and physical properties of indoor hygiene surfaces when assessing their antimicrobial properties.
Collapse
Affiliation(s)
- T. Chang
- Department of Chemistry, KTH Royal Institute of Technology, Div. Surface and Corrosion Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden
- AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, Sweden
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
| | - M. Sepati
- Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Milan, Italy
| | - G. Herting
- Department of Chemistry, KTH Royal Institute of Technology, Div. Surface and Corrosion Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden
| | - C. Leygraf
- Department of Chemistry, KTH Royal Institute of Technology, Div. Surface and Corrosion Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden
| | - G. Kuttuva Rajarao
- Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden
| | - K. Butina
- AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, Sweden
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
| | - A. Richter-Dahlfors
- AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, Sweden
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
- KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Fibre and Polymer Technology, Stockholm, Sweden
| | - E. Blomberg
- Department of Chemistry, KTH Royal Institute of Technology, Div. Surface and Corrosion Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden
| | - I. Odnevall Wallinder
- Department of Chemistry, KTH Royal Institute of Technology, Div. Surface and Corrosion Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden
- AIMES—Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, Sweden
- Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
| |
Collapse
|
|
27
|
Balasubramaniam B, Prateek, Ranjan S, Saraf M, Kar P, Singh SP, Thakur VK, Singh A, Gupta RK. Antibacterial and Antiviral Functional Materials: Chemistry and Biological Activity toward Tackling COVID-19-like Pandemics. ACS Pharmacol Transl Sci 2021; 4:8-54. [PMID: 33615160 PMCID: PMC7784665 DOI: 10.1021/acsptsci.0c00174] [Citation(s) in RCA: 112] [Impact Index Per Article: 28.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/21/2020] [Indexed: 12/12/2022]
Abstract
The ongoing worldwide pandemic due to COVID-19 has created awareness toward ensuring best practices to avoid the spread of microorganisms. In this regard, the research on creating a surface which destroys or inhibits the adherence of microbial/viral entities has gained renewed interest. Although many research reports are available on the antibacterial materials or coatings, there is a relatively small amount of data available on the use of antiviral materials. However, with more research geared toward this area, new information is being added to the literature every day. The combination of antibacterial and antiviral chemical entities represents a potentially path-breaking intervention to mitigate the spread of disease-causing agents. In this review, we have surveyed antibacterial and antiviral materials of various classes such as small-molecule organics, synthetic and biodegradable polymers, silver, TiO2, and copper-derived chemicals. The surface protection mechanisms of the materials against the pathogen colonies are discussed in detail, which highlights the key differences that could determine the parameters that would govern the future development of advanced antibacterial and antiviral materials and surfaces.
Collapse
Affiliation(s)
| | - Prateek
- Department
of Chemical Engineering, Indian Institute
of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Sudhir Ranjan
- Department
of Chemical Engineering, Indian Institute
of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Mohit Saraf
- Department
of Chemical Engineering, Indian Institute
of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Prasenjit Kar
- Department
of Chemical Engineering, Indian Institute
of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Surya Pratap Singh
- Department
of Chemistry, Indian Institute of Technology
Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Vijay Kumar Thakur
- Biorefining
and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Kings Buildings, West Mains Road, Edinburgh EH9 3JG, United Kingdom
| | - Anand Singh
- Department
of Chemistry, Indian Institute of Technology
Kanpur, Kanpur, Uttar Pradesh 208016, India
| | - Raju Kumar Gupta
- Department
of Chemical Engineering, Indian Institute
of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
- Center
for Environmental Science and Engineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India
| |
Collapse
|
|
28
|
The photocatalytic antibacterial behavior of Cu-doped nanocrystalline hematite prepared by mechanical alloying. APPLIED NANOSCIENCE 2021. [DOI: 10.1007/s13204-020-01659-6] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/15/2023]
|
|
29
|
Reactive magnetron co-sputtering of Ti-xCuO coatings: Multifunctional interfaces for blood-contacting devices. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2020; 116:111198. [DOI: 10.1016/j.msec.2020.111198] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/14/2020] [Revised: 05/20/2020] [Accepted: 06/16/2020] [Indexed: 02/08/2023]
|
|
30
|
Zhao J, Yan P, Snow B, Santos RM, Chiang YW. Micro-structured copper and nickel metal foams for wastewater disinfection: proof-of-concept and scale-up. PROCESS SAFETY AND ENVIRONMENTAL PROTECTION : TRANSACTIONS OF THE INSTITUTION OF CHEMICAL ENGINEERS, PART B 2020; 142:191-202. [PMID: 32572308 PMCID: PMC7293508 DOI: 10.1016/j.psep.2020.06.013] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/21/2020] [Revised: 06/05/2020] [Accepted: 06/08/2020] [Indexed: 05/03/2023]
Abstract
It is necessary to disinfect treated wastewater prior to discharge to reduce exposure risks to humans and the environment. The currently practiced wastewater disinfection technologies are challenged by toxic by-products, chemicals and energy demand, a range of effectiveness limitations, among other concerns. An effective, eco-friendly, and energy-efficient alternative disinfection technique is desirable to modernize and enhance wastewater treatment operations. Copper and nickel micro-structured metal foams, and a conventional copper mesh, were evaluated as disinfecting surfaces for treating secondary-treated wastewater contaminated with coliform bacteria. The micro-structured copper foam was adopted for scale-up study, due to its stable and satisfactory bactericidal performance obtained over a wide range of bacterial concentrations and metal-to-liquid ratios. Three scales of experiments, using two types of reactor designs, were performed using municipal wastewater to determine the optimal scale-up factors: small lab-scale batch reactor, intermediate lab-scale batch reactor, and pilot-scale continuous tubular reactor experiments. The performance was evaluated with the aim of minimizing metal material requirement with respect to bactericidal efficiency and leaching risks at all scales. Copper foam, at or above optimal conditions, consistently inactivated over 95 % of total coliforms, fecal coliforms and E.coli in wastewater at various scales, and leachate copper concentrations were determined to be below Canadian guideline values for outfall. This study successfully implemented the "structure" strategy of process intensification, and opens up the possibility to apply micro-structured copper foam in a range of other water disinfection systems, from pre-treatment to point-of-use, and should thus become a topic of further research.
Collapse
Affiliation(s)
- Jinghan Zhao
- School of Engineering, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
| | - Peihua Yan
- School of Engineering, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
| | - Benjamin Snow
- School of Engineering, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
| | - Rafael M Santos
- School of Engineering, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
| | - Yi Wai Chiang
- School of Engineering, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
| |
Collapse
|
|
31
|
Korniienko V, Oleshko O, Husak Y, Deineka V, Holubnycha V, Mishchenko O, Kazek-Kęsik A, Jakóbik-Kolon A, Pshenychnyi R, Leśniak-Ziółkowska K, Kalinkevich O, Kalinkevich A, Pisarek M, Simka W, Pogorielov M. Formation of a Bacteriostatic Surface on ZrNb Alloy via Anodization in a Solution Containing Cu Nanoparticles. MATERIALS 2020; 13:ma13183913. [PMID: 32899716 PMCID: PMC7560052 DOI: 10.3390/ma13183913] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/13/2020] [Revised: 08/26/2020] [Accepted: 08/31/2020] [Indexed: 02/06/2023]
Abstract
High strength, excellent corrosion resistance, high biocompatibility, osseointegration ability, and low bacteria adhesion are critical properties of metal implants. Additionally, the implant surface plays a critical role as the cell and bacteria host, and the development of a simultaneously antibacterial and biocompatible implant is still a crucial challenge. Copper nanoparticles (CuNPs) could be a promising alternative to silver in antibacterial surface engineering due to low cell toxicity. In our study, we assessed the biocompatibility and antibacterial properties of a PEO (plasma electrolytic oxidation) coating incorporated with CuNPs (Cu nanoparticles). The structural and chemical parameters of the CuNP and PEO coating were studied with TEM/SEM (Transmission Electron Microscopy/Scanning Electron Microscopy), EDX (Energy-Dispersive X-ray Dpectroscopy), and XRD (X-ray Diffraction) methods. Cell toxicity and bacteria adhesion tests were used to prove the surface safety and antibacterial properties. We can conclude that PEO on a ZrNb alloy in Ca-P solution with CuNPs formed a stable ceramic layer incorporated with Cu nanoparticles. The new surface provided better osteoblast adhesion in all time-points compared with the nontreated metal and showed medium grade antibacterial activities. PEO at 450 V provided better antibacterial properties that are recommended for further investigation.
Collapse
Affiliation(s)
- Viktoriia Korniienko
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | - Oleksandr Oleshko
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | - Yevheniia Husak
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | - Volodymyr Deineka
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | - Viktoriia Holubnycha
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | | | - Alicja Kazek-Kęsik
- Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland; (A.K.-K.); (A.J.-K.); (K.L.-Z.)
| | - Agata Jakóbik-Kolon
- Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland; (A.K.-K.); (A.J.-K.); (K.L.-Z.)
| | - Roman Pshenychnyi
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
| | - Katarzyna Leśniak-Ziółkowska
- Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland; (A.K.-K.); (A.J.-K.); (K.L.-Z.)
| | | | | | - Marcin Pisarek
- Institute of Physical Chemistry PAS, 01-224 Warsaw, Poland;
| | - Wojciech Simka
- NanoPrime, 39-200 Dębica, Poland;
- Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland; (A.K.-K.); (A.J.-K.); (K.L.-Z.)
- Correspondence: (W.S.); (M.P.); Tel.: +48-32-237-2605 (W.S.)
| | - Maksym Pogorielov
- Medical Institute, Sumy State University, 40018 Sumy, Ukraine; (V.K.); (O.O.); (Y.H.); (V.D.); (V.H.); (R.P.)
- NanoPrime, 39-200 Dębica, Poland;
- Correspondence: (W.S.); (M.P.); Tel.: +48-32-237-2605 (W.S.)
| |
Collapse
|
|
32
|
Maertens L, Coninx I, Claesen J, Leys N, Matroule JY, Van Houdt R. Copper Resistance Mediates Long-Term Survival of Cupriavidus metallidurans in Wet Contact With Metallic Copper. Front Microbiol 2020; 11:1208. [PMID: 32582116 PMCID: PMC7284064 DOI: 10.3389/fmicb.2020.01208] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/18/2020] [Accepted: 05/12/2020] [Indexed: 11/13/2022] Open
Abstract
Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.
Collapse
Affiliation(s)
- Laurens Maertens
- Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium
- Research Unit in Microorganisms Biology (URBM), Narilis Institute, University of Namur, Namur, Belgium
| | - Ilse Coninx
- Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium
| | - Jürgen Claesen
- Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium
| | - Natalie Leys
- Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium
| | - Jean-Yves Matroule
- Research Unit in Microorganisms Biology (URBM), Narilis Institute, University of Namur, Namur, Belgium
| | - Rob Van Houdt
- Microbiology Unit, Interdisciplinary Biosciences, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium
| |
Collapse
|
|
33
|
Mitra D, Kang ET, Neoh KG. Antimicrobial Copper-Based Materials and Coatings: Potential Multifaceted Biomedical Applications. ACS APPLIED MATERIALS & INTERFACES 2020; 12:21159-21182. [PMID: 31880421 DOI: 10.1021/acsami.9b17815] [Citation(s) in RCA: 125] [Impact Index Per Article: 25.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/10/2023]
Abstract
Surface contamination by microbes leads to several detrimental consequences like hospital- and device-associated infections. One measure to inhibit surface contamination is to confer the surfaces with antimicrobial properties. Copper's antimicrobial properties have been known since ancient times, and the recent resurgence in exploiting copper for application as antimicrobial materials or coatings is motivated by the growing concern about antibiotic resistance and the pressure to reduce antibiotic use. Copper, unlike silver, demonstrates rapid and high microbicidal efficacy against pathogens that are in close contact under ambient indoor conditions, which enhances its range of applicability. This review highlights the mechanisms behind copper's potent antimicrobial property, the design and fabrication of different copper-based antimicrobial materials and coatings comprising metallic copper/copper alloys, copper nanoparticles or ions, and their potential for practical applications. Finally, as the antimicrobial coatings market is expected to grow, we offer our perspectives on the implications of increased copper release into the environment and the potential ecotoxicity effects and possibility of development of resistant genes in pathogens.
Collapse
Affiliation(s)
- Debirupa Mitra
- Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 117576
| | - En-Tang Kang
- Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 117576
| | - Koon Gee Neoh
- Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 117576
| |
Collapse
|
|
34
|
Antibacterial Efficiency of Stainless-Steel Grids Coated with Cu-Ag by Thermionic Vacuum Arc Method. COATINGS 2020. [DOI: 10.3390/coatings10040322] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Abstract
Autonomous smart natural ventilation systems (SVS) attached to the glass façade of living quarters and office buildings can help reducing the carbon footprint of city buildings in the future, especially during warm seasons and can represent an alternative to the conventional mechanical ventilation systems. The work performed in this manuscript focuses on the investigation of bacteria trapping and killing efficiency of stainless steel grids coated with a mixed layer of Cu-Ag. These grids are to be employed as decontamination filters for a smart natural ventilation prototype that we are currently building in our laboratory. The tested grids were coated with a mixed Cu-Ag layer using thermionic vacuum arc plasma processing technology. The fixed deposition geometry allowed the variation of Cu and Ag atomic concentration in coated layers as a function of substrate position in relation to plasma sources. The test conducted with air contaminated with a pathogen strain of staphylococcus aureus indicated that the filtering efficiency is influenced by two parameters: the pore size dimension and the coating layer composition. The results show that the highest filtering efficiency of 100% was obtained for fine pore (0.5 × 0.5 mm) grids coated with a mixed metallic layer composed of 65 at% Cu and 35 at% Ag. The second test performed only on reference grids and Cu-Ag (65–35 at%) under working conditions, confirm a similar filtering efficiency for the relevant microbiological markers. This particular sample was investigated from morphological, structural, and compositional point of view. The results show that the layer has a high surface roughness with good wear resistance and adhesion to the substrate. The depth profiles presented a uniform composition of Cu and Ag in the layer with small variations caused by changes in deposition rates during the coating process. Identification of the two metallic phases of the Cu and Ag in the layers evidences their crystalline nature. The calculated grain size of the nanocrystalline was in the range 14–21 nm.
Collapse
|
|
35
|
Zhang X, Yang C, Yang K. Contact Killing of Cu-Bearing Stainless Steel Based on Charge Transfer Caused by the Microdomain Potential Difference. ACS APPLIED MATERIALS & INTERFACES 2020; 12:361-372. [PMID: 31804793 DOI: 10.1021/acsami.9b19596] [Citation(s) in RCA: 29] [Impact Index Per Article: 5.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/03/2023]
Abstract
The addition of copper makes the Cu-bearing stainless steel (SS) possess excellent antibacterial properties. However, the antibacterial mechanism of the Cu-bearing SS is still not accurately understood and recognized. On the one hand, the concentration of released antibacterial Cu ions from its surface is insufficient to generate such an effect. On the other hand, due to the limited Cu content, the area of copper toxicity that can be contacted with bacteria is also much less than that of pure Cu. Therefore, the purpose of this study was to explore the way of bacterial inactivation caused by Cu-bearing SS from the view of the charge transfer. The results showed that the continuous and effective contact between bacteria and Cu-bearing SS is the key to induce the bacteria-killing effect so that the cathode electrons generated by the potential difference of the material microdomain can cause the proton depletion in the bacterial cells, thereby disturbing the respiratory chain and energy generation of the bacterial cells. The proton depletion reaction also catalyzed the conversion of Cu(II) into Cu(I). Cu(I) not only destroys the iron-sulfur protein but also undergoes the redox reaction with Cu(II) to produce reactive oxygen species, causing oxidative damage to cells, eventually accelerating the bacterial death.
Collapse
Affiliation(s)
- Xinrui Zhang
- School of Materials Science and Engineering , University of Science and Technology of China , Shenyang 110016 , China
- Institute of Metal Research , Chinese Academy of Sciences , Shenyang 110016 , China
| | - Chunguang Yang
- Institute of Metal Research , Chinese Academy of Sciences , Shenyang 110016 , China
| | - Ke Yang
- Institute of Metal Research , Chinese Academy of Sciences , Shenyang 110016 , China
| |
Collapse
|
|
36
|
Luo J, Hein C, Pierson JF, Mücklich F. Early-stage corrosion, ion release, and the antibacterial effect of copper and cuprous oxide in physiological buffers: Phosphate-buffered saline vs Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. Biointerphases 2019; 14:061004. [PMID: 31830792 DOI: 10.1063/1.5123039] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/28/2023] Open
Abstract
Copper surfaces are well known for their antibacterial effects due to the release of copper ions. This benefit has been shown in many antibacterial efficiency tests, however, without considering the corrosion behaviors of copper in the physiological solutions, which could play an indispensable role in ion release from the metallic surface. This study compared the ground copper surface and sputtered cuprous oxide (Cu2O) coating in two common physiological buffers: phosphate-buffered saline (PBS) and Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Na-HEPES). The growth of the cuprous oxide (Cu2O) layer was found on copper in pure PBS, inhibiting further copper ion release. In contrast, a continuous release of copper ions was recorded in Na-HEPES for 3 h, where no oxide formation was observed. The antibacterial efficiency of copper (against E. coli) was measured and discussed with the ion release kinetics in the presence of E. coli. Similar results were obtained from Cu2O coating, ruling out its assisting role in showing the antibacterial property from copper surfaces, but they did indicate the importance of taking environmental parameters into consideration in interpreting the antibacterial efficiency of copper surfaces.
Collapse
Affiliation(s)
- Jiaqi Luo
- Functional Materials, Saarland University, 66123 Saarbruecken, Germany
| | - Christina Hein
- Inorganic Solid State Chemistry, Saarland University, 66123 Saarbruecken, Germany
| | | | - Frank Mücklich
- Functional Materials, Saarland University, 66123 Saarbruecken, Germany
| |
Collapse
|
|
37
|
Colin M, Charpentier E, Klingelschmitt F, Bontemps C, De Champs C, Reffuveille F, Gangloff SC. Specific antibacterial activity of copper alloy touch surfaces in five long-term care facilities for older adults. J Hosp Infect 2019; 104:283-292. [PMID: 31809775 DOI: 10.1016/j.jhin.2019.11.021] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2019] [Accepted: 11/25/2019] [Indexed: 12/17/2022]
Abstract
BACKGROUND Pathogens involved in healthcare-associated infections can quickly spread in the environment, particularly to frequently touched surfaces, which can be reservoirs for pathogens. AIM The purpose of this study was to investigate naturally occurring bacterial contamination on touch surfaces in five French long-term care facilities and to compare bacterial populations recovered from copper and control surfaces. METHODS More than 1300 surfaces were sampled. The collected bacteria were identified to obtain a global view of the cultivable bacterial populations colonizing touch surfaces. Haemolytic colonies and putative pathogens were also screened using specific agar plates and then identified with matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. In total, more than 3400 colonies were analysed. FINDINGS Staphylococcus and Micrococcus were the two predominant genera present on touch surfaces, respectively occurring on 51.8% and 48.0% of control surfaces. In these facilities with relatively low bioburden, copper surfaces efficiently reduced the occurrence frequencies of three genera: Staphylococcus, Streptococcus and Roseomonas. Pathogenic species such as Staphylococcus aureus, Enterococcus faecalis and E. faecium were observed in very few samples. In addition, meticillin-resistant S. aureus was observed on five control surfaces and one copper surface. CONCLUSION Contamination of healthcare facilities touch surfaces can be the source for the spread of bacteria through the institution. This in situ study shows that the frequency of the contamination as well as the specific bacterial population bioburden is reduced on copper alloy surfaces.
Collapse
Affiliation(s)
- M Colin
- Université de Reims Champagne-Ardenne, EA 4691 Biomatériaux et Inflammation en site Osseux (BIOS), SFR CAP-Santé, France
| | - E Charpentier
- Université de Reims Champagne-Ardenne, EA 4691 Biomatériaux et Inflammation en site Osseux (BIOS), SFR CAP-Santé, France; Université de Reims Champagne-Ardenne, UFR de Pharmacie, Service de Microbiologie, France
| | - F Klingelschmitt
- Université de Reims Champagne-Ardenne, EA 4691 Biomatériaux et Inflammation en site Osseux (BIOS), SFR CAP-Santé, France
| | - C Bontemps
- Dynamique des génomes et adaptation microbienne, UMR1128, Université de Lorraine, INRA Vandœuvre-lès-Nancy, France
| | - C De Champs
- Université de Reims Champagne-Ardenne, Inserm UMR-S 1250 P3Cell, SFR CAP-Santé, Laboratoire de Bactériologie - Virologie - Hygiène hospitalière, CHU Reims, 51100 Reims, France
| | - F Reffuveille
- Université de Reims Champagne-Ardenne, EA 4691 Biomatériaux et Inflammation en site Osseux (BIOS), SFR CAP-Santé, France; Université de Reims Champagne-Ardenne, UFR de Pharmacie, Service de Microbiologie, France
| | - S C Gangloff
- Université de Reims Champagne-Ardenne, EA 4691 Biomatériaux et Inflammation en site Osseux (BIOS), SFR CAP-Santé, France; Université de Reims Champagne-Ardenne, UFR de Pharmacie, Service de Microbiologie, France.
| |
Collapse
|
|
38
|
Velazquez S, Griffiths W, Dietz L, Horve P, Nunez S, Hu J, Shen J, Fretz M, Bi C, Xu Y, Van Den Wymelenberg KG, Hartmann EM, Ishaq SL. From one species to another: A review on the interaction between chemistry and microbiology in relation to cleaning in the built environment. INDOOR AIR 2019; 29:880-894. [PMID: 31429989 PMCID: PMC6852270 DOI: 10.1111/ina.12596] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/10/2019] [Revised: 08/11/2019] [Accepted: 08/15/2019] [Indexed: 05/12/2023]
Abstract
Since the advent of soap, personal hygiene practices have revolved around removal, sterilization, and disinfection-both of visible soil and microscopic organisms-for a myriad of cultural, aesthetic, or health-related reasons. Cleaning methods and products vary widely in their recommended use, effectiveness, risk to users or building occupants, environmental sustainability, and ecological impact. Advancements in science and technology have facilitated in-depth analyses of the indoor microbiome, and studies in this field suggest that the traditional "scorched-earth cleaning" mentality-that surfaces must be completely sterilized and prevent microbial establishment-may contribute to long-term human health consequences. Moreover, the materials, products, activities, and microbial communities indoors all contribute to, or remove, chemical species to the indoor environment. This review examines the effects of cleaning with respect to the interaction of chemistry, indoor microbiology, and human health.
Collapse
Affiliation(s)
| | - Willem Griffiths
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
| | - Leslie Dietz
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
| | - Patrick Horve
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
| | - Susie Nunez
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
| | - Jinglin Hu
- Department of Civil and Environmental EngineeringNorthwestern UniversityEvanstonIL
| | - Jiaxian Shen
- Department of Civil and Environmental EngineeringNorthwestern UniversityEvanstonIL
| | - Mark Fretz
- Institute for Health and the Built EnvironmentUniversity of OregonPortlandOR
| | - Chenyang Bi
- Department of Civil Environmental EngineeringVirginia Polytechnic Institute and State UniversityBlacksburgVA
| | - Ying Xu
- Department of Building ScienceTsinghua UniversityBeijingChina
| | - Kevin G. Van Den Wymelenberg
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
- Institute for Health and the Built EnvironmentUniversity of OregonPortlandOR
| | - Erica M. Hartmann
- Department of Civil and Environmental EngineeringNorthwestern UniversityEvanstonIL
| | - Suzanne L. Ishaq
- Biology and the Built Environment CenterUniversity of OregonEugeneOR
| |
Collapse
|
|
39
|
Luo J, Hein C, Ghanbaja J, Pierson JF, Mücklich F. Bacteria accumulate copper ions and inhibit oxide formation on copper surface during antibacterial efficiency test. Micron 2019; 127:102759. [PMID: 31585250 DOI: 10.1016/j.micron.2019.102759] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/14/2019] [Revised: 09/23/2019] [Accepted: 09/23/2019] [Indexed: 12/11/2022]
Abstract
Copper surface after antibacterial test against E. coli was examined in the aspect of corrosion. Results from scanning electron microscope (SEM), grazing incidence X-ray diffractometer (GIXRD) and Raman spectroscopy together confirmed less oxidation on copper surface with the presence of E. coli. The inhibition of the cuprous oxide (Cu2O) layer instead ensured the continuous exposure of copper surface, letting localised corrosion attacks observable and causing a stronger release of copper ions. These phenomena are attributed to the fact that E. coli act as ions reservoirs since high amount of copper accumulation were found by energy dispersive X-ray spectroscopy (EDS).
Collapse
Affiliation(s)
- Jiaqi Luo
- Functional Materials, Saarland University, Germany; Université de Lorraine, CNRS, IJL, F-54000, Nancy, France.
| | - Christina Hein
- Inorganic Solid State Chemistry, Saarland University, Germany
| | | | | | | |
Collapse
|
|
40
|
The Use of Copper as an Antimicrobial Agent in Health Care, Including Obstetrics and Gynecology. Clin Microbiol Rev 2019; 32:32/4/e00125-18. [PMID: 31413046 DOI: 10.1128/cmr.00125-18] [Citation(s) in RCA: 77] [Impact Index Per Article: 12.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022] Open
Abstract
Health care-associated infections (HAIs) are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial-resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper-coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. This review provides an overview of the historical and current evidence of the antimicrobial and wound-healing properties of copper and explores its possible utility in obstetrics and gynecology.
Collapse
|
|
41
|
Ghosh R, Swart O, Westgate S, Miller BL, Yates MZ. Antibacterial Copper-Hydroxyapatite Composite Coatings via Electrochemical Synthesis. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2019; 35:5957-5966. [PMID: 30951314 DOI: 10.1021/acs.langmuir.9b00919] [Citation(s) in RCA: 28] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/09/2023]
Abstract
Antibacterial copper-hydroxyapatite (Cu-HA) composite coatings on titanium were synthesized using a novel process consisting of two consecutive electrochemical reactions. In the first stage, HA nanocrystals were grown on titanium using the cathodic electrolytic synthesis. The HA-coated titanium was then used as the cathode in a second reaction stage to electrochemically reduce Cu2+ ions in solution to metallic Cu nanoparticles. Reaction conditions were found that result in nanoscale Cu particles growing on the surface of the HA crystals. The two-stage synthesis allows facile control of copper content in the HA coatings. Antibacterial activity was measured by culturing Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) in the presence of coatings having varying copper contents. The coatings displayed copper concentration-dependent antibacterial activity against both types of bacteria, likely due to the slow release of copper ions from the coatings. The observation of antibacterial activity from a relatively low loading of copper on the bioactive HA support suggests that multifunctional implant coatings can be developed to supplement or supplant prophylactic antibiotics used in implant surgery that are responsible for creating resistant bacteria strains.
Collapse
Affiliation(s)
- Rashmi Ghosh
- Department of Chemical Engineering , University of Rochester , Rochester , New York 14623 , United States
| | - Oliver Swart
- Department of Dermatology , University of Rochester , Rochester , New York 14611 , United States
| | - Sabrina Westgate
- Department of Chemical Engineering , University of Rochester , Rochester , New York 14623 , United States
| | - Benjamin L Miller
- Department of Dermatology , University of Rochester , Rochester , New York 14611 , United States
| | - Matthew Z Yates
- Department of Chemical Engineering , University of Rochester , Rochester , New York 14623 , United States
- Laboratory for Laser Energetics , University of Rochester , Rochester , New York 14627 , United States
| |
Collapse
|
|
42
|
Bukhtiyarova PA, Antsiferov DV, Brasseur G, Avakyan MR, Frank YA, Ikkert OP, Pimenov NV, Tuovinen OH, Karnachuk OV. Isolation, characterization, and genome insights into an anaerobic sulfidogenic Tissierella bacterium from Cu-bearing coins. Anaerobe 2019; 56:66-77. [PMID: 30776428 DOI: 10.1016/j.anaerobe.2019.02.012] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2018] [Revised: 01/27/2019] [Accepted: 02/14/2019] [Indexed: 01/19/2023]
Abstract
Recent reports on antimicrobial effects of metallic Cu prompted this study of anaerobic microbial communities on copper surfaces. Widely circulating copper-containing coinage was used as a potential source for microorganisms that had had human contact and were tolerant to copper. This study reports on the isolation, characterization, and genome of an anaerobic sulfidogenic Tissierella sp. P1from copper-containing brass coinage. Dissimilatory (bi)sulfite reductase dsrAB present in strain P1 genome and the visible absorbance around 630 nm in the cells suggested the presence of a desulfoviridin-type protein. However, the sulfate reduction rate measurements with 35SO42- did not confirm the dissimilatory sulfate reduction by the strain. The P1 genome lacks APS reductase, sulfate adenylyltransferase, DsrC, and DsrMK necessary for dissimilatory sulfate reduction. The isolate produced up to 0.79 mM H2S during growth, possibly due to cysteine synthase (CysK) and/or cysteine desulfhydrase (CdsH) activities, encoded in the genome. The strain can tolerate up to 2.4 mM Cu2+(150 mg/l) in liquid medium, shows affinity to metallic copper, and can survive on copper-containing coins up to three days under ambient air and dry conditions. The genome sequence of strain P1 contained cutC, encoding a copper resistance protein, which distinguishes it from all other Tissierella strains with published genomes.
Collapse
Affiliation(s)
- Polina A Bukhtiyarova
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia
| | - Dmitry V Antsiferov
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia
| | - Gael Brasseur
- Laboratoire de Chimie Bactérienne, CNRS, Mediterranean Institute of Microbiology, Marseille, France
| | - Marat R Avakyan
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia
| | - Yulia A Frank
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia
| | - Olga P Ikkert
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia
| | - Nikolay V Pimenov
- Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia
| | - Olli H Tuovinen
- Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA
| | - Olga V Karnachuk
- Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Tomsk, Russia.
| |
Collapse
|
|
43
|
Antiparasitic Effect of Copper Alloy Surface on Cryptocaryon irritans in Aquaculture of Larimichthys crocea. Appl Environ Microbiol 2019; 85:AEM.01982-18. [PMID: 30446561 DOI: 10.1128/aem.01982-18] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2018] [Accepted: 11/13/2018] [Indexed: 01/06/2023] Open
Abstract
Copper and alloys containing >60% copper by weight are antimicrobial. In aquaculture, copper alloys are used as part of corrosion-resistant cages or as part of copper coating. To test whether a copper alloy surface prevents the outbreak of parasitosis in the aquaculture of Larimichthys crocea, we covered the bottom of the aquaculture tank with sheets of copper alloy containing 74% to 78% copper, and we cultured L. crocea juveniles that had been artificially infected with the protozoan parasite Cryptocaryon irritans Our results showed that these copper alloy sheets effectively blocked the infectious cycle of C. irritans within a 1-week period and significantly reduced the number of C. irritans trophonts and tomonts, thereby decreasing the mortality rate of L. crocea In in vitro assays, the cytoplasmic membranes of protomonts disintegrated and the cytoplasm overflowed after just 5 minutes of contact with copper alloy surfaces. Although the same cytoplasmic membrane disintegration was not observed in tomonts, the tomonts completely lost their capacity for proliferation and eventually died following direct contact with copper alloy sheets for 1 h; this is likely because C. irritans tomonts took in >100 times more copper ions following contact with the copper alloy sheets than within the control aquaculture environment. Exposure to copper alloy sheets did not lead to excessive heavy metal levels in the aquacultured fish or in the culture seawater.IMPORTANCE Cryptocaryon irritans, a parasitic ciliate that penetrates the epithelium of the gills, skin, and fins of marine fish, causes acute suffocation and death in cultured fish within days of infection. Much of the existing research centers around the prevention of C. irritans infection, but no cure has been found. Studies demonstrate that copper has strong antimicrobial properties, and fish grown in copper-containing cages have lower rates of C. irritans infection, compared to those grown in other currently used aquaculture cages. In this study, we found that an alloy containing 74% to 78% copper by weight effectively killed C. irritans cells and prevented cryptocaryoniasis outbreaks within a 1-week period. These findings offer a new perspective on the prevention and control of cryptocaryoniasis.
Collapse
|
|
44
|
Hao Y, Huang X, Zhou X, Li M, Ren B, Peng X, Cheng L. Influence of Dental Prosthesis and Restorative Materials Interface on Oral Biofilms. Int J Mol Sci 2018; 19:E3157. [PMID: 30322190 PMCID: PMC6213966 DOI: 10.3390/ijms19103157] [Citation(s) in RCA: 108] [Impact Index Per Article: 15.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/27/2018] [Revised: 09/29/2018] [Accepted: 10/10/2018] [Indexed: 01/17/2023] Open
Abstract
Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exposed to oral conditions can influence pellicle coating, initial bacterial adhesion, and biofilm formation, due to their specific physical and chemical characteristics. To define the effect of physical and chemical characteristics of dental prosthesis and restorative material on oral biofilms, we discuss resin-based composites, glass ionomer cements, amalgams, dental alloys, ceramic, and dental implant material surface properties. In conclusion, each particular chemical composition (organic matrix, inorganic filler, fluoride, and various metallic ions) can enhance or inhibit biofilm formation. Irregular topography and rough surfaces provide favorable interface for bacterial colonization, protecting bacteria against shear forces during their initial reversible binding and biofilm formation. Moreover, the surface free energy, hydrophobicity, and surface-coating techniques, also have a significant influence on oral biofilms. However, controversies still exist in the current research for the different methods and models applied. In addition, more in situ studies are needed to clarify the role and mechanism of each surface parameter on oral biofilm development.
Collapse
Affiliation(s)
- Yu Hao
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Xiaoyu Huang
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Xuedong Zhou
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Mingyun Li
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Biao Ren
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Xian Peng
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| | - Lei Cheng
- State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
- Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
- National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China.
| |
Collapse
|
|
45
|
Wehbe M, Leung AWY, Abrams MJ, Orvig C, Bally MB. A Perspective - can copper complexes be developed as a novel class of therapeutics? Dalton Trans 2018; 46:10758-10773. [PMID: 28702645 DOI: 10.1039/c7dt01955f] [Citation(s) in RCA: 126] [Impact Index Per Article: 18.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
Abstract
Although copper-ligand complexes appear to be promising as a new class of therapeutics, other than the family of copper(ii) coordination compounds referred to as casiopeínas these compounds have yet to reach the clinic for human use. The pharmaceutical challenges associated with developing copper-based therapeutics will be presented in this article along with a discussion of the potential for high-throughput chemistry, computer-aided drug design, and nanotechnology to address the development of this important class of drug candidates.
Collapse
Affiliation(s)
- Mohamed Wehbe
- Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
| | | | | | | | | |
Collapse
|
|
46
|
Steinhauer K, Meyer S, Pfannebecker J, Teckemeyer K, Ockenfeld K, Weber K, Becker B. Antimicrobial efficacy and compatibility of solid copper alloys with chemical disinfectants. PLoS One 2018; 13:e0200748. [PMID: 30096209 PMCID: PMC6086424 DOI: 10.1371/journal.pone.0200748] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/23/2018] [Accepted: 06/06/2018] [Indexed: 11/19/2022] Open
Abstract
INTRODUCTION Chemical disinfection is state of the art in preventing spread of infectious agents in the healthcare setting. Additionally, the antimicrobial properties of solid copper alloy surfaces against various microorganisms have recently been substantiated. Thus, antimicrobially active copper surfaces may serve as an additional barrier against distribution of pathogenic microorganisms and be combined with chemical disinfection measures in the hospital. The aim of this study was therefore to investigate on a quantitative basis whether the combination of chemical disinfectants with copper alloy surfaces results in an overall compromised, combined or even synergistic antimicrobial efficacy. METHODS Experiments were carried out using the quantitative carrier test devised by the German Society for Hygiene and Microbiology (DGHM) to study antimicrobial efficacy of chemical disinfectants. Requirements for microbicidal efficacy as defined by prEN 14885 were applied. The chemical disinfectants tested in our study contained alcohols (ethanol, 1-propanol), quaternary ammonium compounds (benzalkonium chloride) and glutaraldehyde as actives. Quantitative carrier tests were carried out on different carriers (tiles, copper alloy discs, stainless steel discs) using Pseudomonas aeruginosa, Staphylococcus aureus, Kocuria rhizophila and Candida albicans as test organisms. RESULTS For the alcohol-based disinfectant no difference in antimicrobial efficacy was observed when applied to antimicrobial active copper alloy carriers, tiles or stainless steel discs. For all test organisms microbial contamination was reduced to the detection limit of < 1 log (CFU/ml) within a contact time of 2 min indicating a ≥ 5 log reduction for the tested bacteria and a ≥ 4 log reduction for the yeast, as being requested for chemical disinfectants by prEN 14885. In order to elucidate a potential synergism the chemical disinfectant based on quaternary ammonium compounds (benzalkonium chloride) and glutaraldehyde was used at a sub-effective concentration. Hence, no complete reduction of microbial contamination was achieved on stainless steel or tile carriers for Pseudomonas aeruginosa and Candida albicans. Interestingly, when using copper alloy carriers complete reduction indicating a ≥ 5 log reduction for P. aeruginosa and a ≥ 4 log reduction for C. albicans was detected. Thus, data of this study indicates that solid copper alloy surfaces and disinfectants synergize. CONCLUSIONS According to this data, commercially available disinfectants based on alcohol, quaternary ammonium compounds and aldehyde can effectively be combined in a dual strategy with solid copper alloy surfaces to reduce microbial contamination.
Collapse
Affiliation(s)
- Katrin Steinhauer
- Research & Regulatory Affairs, Schülke & Mayr GmbH, Norderstedt, Germany
- * E-mail:
| | - Sonja Meyer
- Ostwestfalen-Lippe University of Applied Sciences, Department Life Science Technologies, Microbiology, Lemgo, Germany
| | - Jens Pfannebecker
- Ostwestfalen-Lippe University of Applied Sciences, Department Life Science Technologies, Microbiology, Lemgo, Germany
| | - Karin Teckemeyer
- Research & Regulatory Affairs, Schülke & Mayr GmbH, Norderstedt, Germany
| | - Klaus Ockenfeld
- Environment and Health, Deutsches Kupferinstitut Berufsverband e.V., Düsseldorf, Germany
| | - Klaus Weber
- Research & Regulatory Affairs, Schülke & Mayr GmbH, Norderstedt, Germany
| | - Barbara Becker
- Ostwestfalen-Lippe University of Applied Sciences, Department Life Science Technologies, Microbiology, Lemgo, Germany
| |
Collapse
|
|
47
|
Antimicrobial properties of ternary eutectic aluminum alloys. Biometals 2018; 31:759-770. [PMID: 29946993 DOI: 10.1007/s10534-018-0119-1] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/20/2017] [Accepted: 06/12/2018] [Indexed: 01/05/2023]
Abstract
Several Escherichia coli deletion mutants of the Keio collection were selected for analysis to better understand which genes may play a key role in copper or silver homeostasis. Each of the selected E. coli mutants had a deletion of a single gene predicted to encode proteins for homologous recombination or contained functions directly linked to copper or silver transport or transformation. The survival of these strains on pure copper surfaces, stainless steel, and alloys of aluminum, copper and/or silver was investigated. When exposed to pure copper surfaces, E. coli ΔcueO was the most sensitive, whereas E. coli ΔcopA was the most resistant amongst the different strains tested. However, we observed a different trend in sensitivities in E. coli strains upon exposure to alloys of the system Al-Ag-Cu. While minor antimicrobial effects were detected after exposure of E. coli ΔcopA and E. coli ΔrecA to Al-Ag alloys, no effect was detected after exposure to Al-Cu alloys. The release of copper ions and cell-associated copper ion concentrations were determined for E. coli ΔcopA and the wild-type E. coli after exposure to pure copper surfaces. Altogether, compared to binary alloys, ternary eutectic alloys (Al-Ag-Cu) had the highest antimicrobial effect and thus, warrant further investigation.
Collapse
|
|
48
|
Wang H, Tang Y, Xia X, Lu Y. Role of poly(ethylene oxide) in copper-containing composite used for intrauterine contraceptive devices. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2018; 29:92. [PMID: 29938314 DOI: 10.1007/s10856-018-6103-z] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/12/2018] [Accepted: 06/05/2018] [Indexed: 06/08/2023]
Abstract
Copper-containing composite is a cupric ions release system to prepare a novel copper intrauterine devices (Cu-IUDs), its biocompatibility and weight of the prepared composite Cu-IUDs are directly relevant to its such side-effects as pain and bleeding. To improve its biocompatibility and reduce its weight of such a composite Cu-IUDs, a copper-containing composite based on polymer alloy of poly(ethylene oxide) (PEO) and low-density polyethylene (LDPE) is developed. Here the role of its PEO in this novel cupric ions release system is reported. The results show that its cupric ions release rate can be adjusted easily by only changing its PEO content, and it increases remarkably with the increase of its PEO content. Our study also show that this influence is caused by the improvement of its hydrophilicity and the formation of its porous structure owing to the introduction of PEO. The improvement of its hydrophilicity make it easier for the surrounding aqueous solution to infiltrate into the composite, and the formation of its porous structure provide more routes for entry of the aqueous solution and diffusion of the released cupric ions. All these results indicate that the Cu/PEO/LDPE composite is a potential material that can be used to prepare such cupric ions release micro-devices as Cu-IUDs with slighter side-effects through its smaller weight.
Collapse
Affiliation(s)
- Huan Wang
- State Key Laboratory of Material Processing and Die & Mould Technology, Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China
| | - Ying Tang
- Institute of Chemical Materials, Chinese Academy of Engineering and Physics, Mianyang, Sichuan, 621900, China
| | - Xianping Xia
- State Key Laboratory of Material Processing and Die & Mould Technology, Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
| | - Yi Lu
- State Key Laboratory of Material Processing and Die & Mould Technology, Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China
| |
Collapse
|
|
49
|
Rosenberg M, Vija H, Kahru A, Keevil CW, Ivask A. Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces. Sci Rep 2018; 8:8172. [PMID: 29802355 PMCID: PMC5970231 DOI: 10.1038/s41598-018-26391-8] [Citation(s) in RCA: 35] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/14/2017] [Accepted: 05/11/2018] [Indexed: 11/09/2022] Open
Abstract
Release of metal ions from metal-based surfaces has been considered one of the main drivers of their antimicrobial activity. Here we describe a method that enables parallel assessment of metal ion release from solid metallic surfaces and antimicrobial efficacy of these surfaces in a short time period. The protocol involves placement of a small volume of bioluminescent bacteria onto the tested surface and direct measurement of bioluminescence at various time points. In this study, two recombinant Escherichia coli strains, one expressing bioluminescence constitutively and applicable for general antimicrobial testing, and the other induced by Cu ions, were selected. Decrease in bioluminescence of constitutive E. coli on the surfaces showed a good correlation with the decrease in bacterial viability. Response of Cu-inducible E. coli showed a correlation with Cu content in the tested surfaces but not with Cu dissolution suggesting the role of direct bacteria-surface contact in Cu ion-driven antibacterial effects. In summary, the presented protocol enables the analysis of microbial toxicity and bioavailability of surface-released metal ions directly on solid surfaces within 30-60 min. Although optimized for copper and copper alloy surfaces and E. coli, the method can be extended to other types of metallic surfaces and bacterial strains.
Collapse
Affiliation(s)
- Merilin Rosenberg
- Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.,Department of Natural Sciences, Tallinn University of Technology, Tallinn, Estonia
| | - Heiki Vija
- Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia
| | - Anne Kahru
- Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.,Estonian Academy of Sciences, Kohtu 6, Tallinn, Estonia
| | - C William Keevil
- Faculty of Natural and Environmental Sciences, Centre for Biological Sciences, University of Southampton, Southampton, UK.
| | - Angela Ivask
- Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
| |
Collapse
|
|
50
|
Meininger M, Meininger S, Groll J, Gbureck U, Moseke C. Silver and copper addition enhances the antimicrobial activity of calcium hydroxide coatings on titanium. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2018; 29:61. [PMID: 29736777 DOI: 10.1007/s10856-018-6065-1] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/08/2017] [Accepted: 04/18/2018] [Indexed: 06/08/2023]
Abstract
Electrochemically assisted deposition of Ca(OH)2 (Portlandite) coatings on titanium surfaces has been proven as a promising method to provide the substrate with a most desirable combination of significant bacterial growth reduction on one hand and good biocompatibility on the other. Due to the rapid in vivo transformation of Ca(OH)2 to hydroxyapatite, the antimicrobial activity will be an ephemeral property of the coating when implanted into the human body. In this study, the ability to reduce bacterial growth of such portlandite coatings was significantly enhanced by an ionic modification with copper and silver ions. Antibacterial tests revealed a noticeably elevated reduction of bacterial growth, especially for copper and even at a relatively low copper content of about 0.3 wt.%. In addition, the cytocompatibility, a crucial prerequisite for potential in vivo biocompatibility, of the copper-modified coating was comparable to pure calcium hydroxide coatings.
Collapse
Affiliation(s)
- M Meininger
- Department for Functional Materials in Medicine and Dentistry, School of Dentistry, University of Würzburg, Pleicherwall 2, D-97070, Würzburg, Germany
| | - S Meininger
- Department for Functional Materials in Medicine and Dentistry, School of Dentistry, University of Würzburg, Pleicherwall 2, D-97070, Würzburg, Germany
| | - J Groll
- Department for Functional Materials in Medicine and Dentistry, School of Dentistry, University of Würzburg, Pleicherwall 2, D-97070, Würzburg, Germany
| | - U Gbureck
- Department for Functional Materials in Medicine and Dentistry, School of Dentistry, University of Würzburg, Pleicherwall 2, D-97070, Würzburg, Germany.
| | - C Moseke
- Institute for Biomedical Engineering (IBMT), University of Applied Sciences Mittelhessen (THM), Wiesenstraße 14, D-35390, Gießen, Germany
| |
Collapse
|