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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Exp Med. Nov 20, 2016; 6(4): 63-71
Published online Nov 20, 2016. doi: 10.5493/wjem.v6.i4.63
Published online Nov 20, 2016. doi: 10.5493/wjem.v6.i4.63
Lipofuscins prepared by modification of photoreceptor cells via glycation or lipid peroxidation show the similar phototoxicity
Alexander Dontsov, Anna Koromyslova, Mikhail Ostrovsky, Natalia Sakina, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia
Author contributions: All authors contributed to this manuscript.
Supported by The Russian Foundation for Basic Research, No. 15-29-03831.
Institutional review board statement: The study reviewed and approved by the Emanuel Institute of Biochemical Physics.
Institutional animal care and use committee statement: The study reviewed and approved by the Emanuel Institute of Biochemical Physics. Animals subjects were not involved in our experimental study.
Conflict-of-interest statement: The authors have no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Natalia Sakina, PhD, Senior Researcher, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygina st. 4, 119334 Moscow, Russia. nsakina@mail.ru
Telephone: +7-495-9397422 Fax: +7-499-1374101
Received: June 29, 2016
Peer-review started: July 1, 2016
First decision: September 5, 2016
Revised: September 19, 2016
Accepted: October 17, 2016
Article in press: October 19, 2016
Published online: November 20, 2016
Peer-review started: July 1, 2016
First decision: September 5, 2016
Revised: September 19, 2016
Accepted: October 17, 2016
Article in press: October 19, 2016
Published online: November 20, 2016
Core Tip
Core tip: The aim of this work is to investigate the influence of different ways of protein-lipid modification of photoreceptor outer segments (POS) on the spectral characteristics and toxicity of lipofuscin. Therefore, model lipofuscins were prepared by protein-lipid modification of POS with products of lipid peroxidation or glycation reaction and the subsequent addition of the fluorophore A2E. The type of photoreceptor modification has no effect on model lipofuscins phototoxicity.