Copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Alendronate disturbs femoral growth due to changes during immunolocalization of transforming growth factor-β1 and bone morphogenetic protein-2 in epiphyseal plate
Juliana Souza Vieira, Emanuelle Juliana Cunha, Luis Henrique Koeler Chaves, Jessica Lakes de Souza, Allan Fernando Giovanini, Clinical Dentistry, University Positivo, Curitiba, PR 81280-330, Brazil
Juliana Feltrin de Souza, Stomatology Department, University Federal of Paraná, Curitiba, PR 80210-170, Brazil
Author contributions: Vieira JS and Cunha EJ performed equally the majority of laboratorial experiments; de Souza JF, Chaves LHK and de Souza JL participated in treatment of animals and performed the immunohistochemical investigations; Giovanini AF analyzed all data and wrote the paper.
Institutional animal care and use committee statement: The principles of laboratory animal care and national laws on animal use were observed for the present study with the approval of the Ethical Committee for Animal Research of Positive University (Protocol #266).
Conflict-of-interest statement: Nothing to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Allan Fernando Giovanini, MD, PhD, Associate Professor, Clinical Dentistry, University Positivo, R Pedro Viriato Parigot de Souza, 5300 Campo Comprido, Curitiba, PR 81280-330, Brazil. afgiovanini@gmail.com
Received: May 6, 2019
Peer-review started: May 6, 2019
First decision: September 2, 2019
Revised: November 26, 2019
Accepted: December 14, 2019
Article in press: December 14, 2019
Published online: January 8, 2020
Peer-review started: May 6, 2019
First decision: September 2, 2019
Revised: November 26, 2019
Accepted: December 14, 2019
Article in press: December 14, 2019
Published online: January 8, 2020
Core Tip
Core tip: This is the first study that addresses the likely action of alendronate in appendicular bone development. Through of macroscopic, immunohistochemical and histological analysis, we revealed that alendronatemay impairs the bone growth, since alters the usual development of epiphyseal plate associated to changes in the immunolocalization of transforming growth factor-β1 and bone morphogenetic protein-2.