Basic Study
Copyright ©The Author(s) 2017.
World J Exp Med. Aug 20, 2017; 7(3): 84-96
Published online Aug 20, 2017. doi: 10.5493/wjem.v7.i3.84
Figure 3
Figure 3 Increased percentage of CD4+CD25-Foxp3+ T cells within diffuse cutaneous systemic sclerosis phenotype vs healthy controls. A: Increased percentage of CD4+CD25-Foxp3+ T cells within dcSSc patients (n = 13) as opposed to controls (n = 16), respectively, 10.94% ± 1.65% vs 6.88% ± 0.91%, P = 0.032. Boxplots are expressed as means ± SD; B-D: Panel B depicts the flow cytometric analysis of CD4+CD25-Foxp3+ T cells. A representative patient with dcSSc phenotype is shown the lymphocytes were gated according to their physical characteristics (FSC and SSC) in R1; afterwards T helper cells were gated in R2. T helpers, which were found negative for CD25 surface expression and positive for Foxp3 intracellular expression (R3, upper left quadrant) were defined as CD4+CD25-Foxp3+ T cells. dcSSc: Diffuse cutaneous systemic sclerosis.