Intracellular localisation of Mycobacterium marinum in mast cells

Table 1 Lactate dehydrogenase release was measured photometrically

Mast cells infected with M.marinumat different MOIs for 8 h	LDH release, % increase over uninfected control
0.5:1 (bacteria:cell)	4.20%
10:1 (bacteria:cell)	31.10%
25:1 (bacteria:cell)	43.70%

Mean of triplicate determinations was related to the control and expressed as % increase. M. marinum: Mycobacterium marinum; LDH: Lactate dehydrogenase; MOI: Multiplicity of infection.

Table 2 A representative flow cytometric analysis of apoptotic (Annexin V⁺/PI^-, early; Annexin V⁺/PI⁺; late) and necrotic (Annexin V^-/PI⁺) human mast cell line cells after 8 h infection with Mycobacterium marinum

Uninfected/infectedHMC-1	Annexin V-/PI-	Annexin V+/PI-	Annexin V+/PI+	Annexin V-/PI+
Uninfected (control)	99.80%	0.20%	0%	0%
Infected with M. marinum at MOI 10	58.52%	31.76%	2.98%	6.70%
Infected with M. marinum at MOI 25	58.24%	29.37%	4.25%	8.14%

Percentage gated population. HMC-1: Human mast cell line; MOI: Multiplicity of infection; M. marinum: Mycobacterium marinum.