Tumor antigen and MHC expression in glioma cells for immunotherapeutic interventions

Table 1 Antibodies for flow cytometry

Host	Antigen	Clone	Label	Company
Mouse	HLA-ABC	W6/32	FITC	Immunotools
Mouse	HLA-DR, -DP	HL-38	FITC	Immunotools
Mouse	HLA-E	3D12	PE	Bio Legend (London, United Kiongdom)
Mouse	HLA-G	87G	APC	BioLegend
Mouse	β2-microglobulin	2M2	FITC	BioLegend
Mouse	MIC A	2C10	FITC	Santa Cruz (Santa Cruz, United States)
Mouse	MIC B	Jan-47	None	Santa Cruz
Humanized	EGFR	Cetuximab	None	Bristol-Myers Squibb (New York, United States)
Humanized	HER2/neu	Trastuzumab	None	Roche (Basel, Switzerland)
Mouse	ICAM-1	1H4	APC	Immunotools
Rabbit	IL-13Rα	Polyclonal	None	AssaybioTech (Sunnyvale, United States)
Mouse	MAGE-1	MA454	None	Thermo Scientific (Waltham, United States)
Mouse	MART-1	M2-7C10	None	Thermo Scientific
Mouse	RHAMM	2D6	None	Leica (Wetzlar, Germany)
Mouse	survivin	3F342	None	Santa Cruz
Mouse	tyrosinase	T311	None	Thermo Scientific
Mouse	WT-1	6F-H2	None	Chemicon international (Temecula, United States)

PE: R-phycoerythrin; APC: Allophycocyanin; FITC: Fluorescein isothiocyanate.

Table 2 HLA-A and A02 expression

	HROG02		HROG04		HROG05		HROG06		HROG10		HROG13		HROG17
HLA-A typing	*01	*02	*01	*02	*02	-	*01	*03	*02	*23	*02	-	*11	*66
Flow cytometry (HLA-A02)	88% ( ± 10%)		95% ( ± 1%)		83% ( ± 10%)		1% ( ± 1%)		89% ( ± 4%)		92% ( ± 3%)		0% ( ± 0%)

In the upper row the results of a 2-digits encompassing HLA typing for the loci HLA-A are listed. Information on both alleles is provided; in case of homozygosity the “second” allele is marked by. In the lower row the mean percentage (of three repetitions) of cells stained positive for HLA-A02 in flow cytometry is given and the SD is provided in brackets.