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Deepika K, Kumar NGR, Kumar AS, Karri RL. Immunohistochemical evaluation of Glut1 in dentigerous cysts, odontogenic keratocysts, and ameloblastoma. J Oral Maxillofac Pathol 2024; 28:77-83. [PMID: 38800443 PMCID: PMC11126251 DOI: 10.4103/jomfp.jomfp_455_23] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2023] [Revised: 12/08/2023] [Accepted: 12/09/2023] [Indexed: 05/29/2024] Open
Abstract
Context Glucose uptake may be considered the rate-limiting step for the growth and metabolism of the cancer cell. Studies on GLUT1 have shown that GLUT1 is involved in cell survival and proliferation in both healthy and pathological circumstances. GLUT1 expression is regarded as one of the crucial elements in the development of local aggressiveness, tumour invasiveness, and metastasis, particularly in malignant tumours. The role of glut1 in odontogenic cysts and tumours has remained uncertain. Aim The aim of the study is to assess the expression of Glut1 in dentigerous cysts, odontogenic keratocysts, and ameloblastoma. Settings and Design The study was conducted in GSL Dental College. The study design was a resprospective immunohistochemical study. Methods and Material Formalin-fixed, paraffin-embedded blocks of histologically confirmed cases (n = 50), 10 cases of odontogenic keratocysts, dentigerous cysts, ameloblastomas solid, ameloblastomas unicystic, and dental follicles each. Brown colour staining was considered as positive staining for GLUT1. Quantitative analysis was performed by counting the number of labelled cells, and semi-quantitative analysis was conducted by assigning immunostaining intensity scores. Statistical Analysis Chi-square test was used to compare differences between the groups. A P value of ≤0.05 was considered as statistically significant. Results Odontogenic keratocysts and unicystic ameloblastoma showed ≥50% of label cells with strong intensity of staining. Odontogenic keratocysts and solid ameloblastoma showed sub-cellular localisation of staining in the cytoplasm and membrane. Dentigerous cysts exhibited combined nucleus, cytoplasm, and membrane sub-cellular localisation of staining. Conclusions The development of ameloblastomas, odontogenic keratocysts, and dentigerous cysts appears to be influenced by GLUT-1. Variation in its expression may aid in explanation of some of the differences in biological activity of these lesions.
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Affiliation(s)
- K Deepika
- Department of Oral and Maxillofacial Pathology, GSL Dental College, Rajahmundry, Andhra Pradesh, India
| | - N. Govind Raj Kumar
- Department of Oral and Maxillofacial Pathology, GSL Dental College, Rajahmundry, Andhra Pradesh, India
| | - A. Sudarshan Kumar
- Department of Oral and Maxillofacial Pathology, GSL Dental College, Rajahmundry, Andhra Pradesh, India
| | - Roja Lakshmi Karri
- Department of Oral and Maxillofacial Pathology, GSL Dental College, Rajahmundry, Andhra Pradesh, India
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Mallick M, Yoithap Prabhunath TR, Kumari S, Sobhia ME. An in silico study of protein-protein interactions and design of novel peptides for TrkA in ameloblastoma. J Biomol Struct Dyn 2023; 42:13768-13778. [PMID: 37975413 DOI: 10.1080/07391102.2023.2278083] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/11/2023] [Accepted: 10/26/2023] [Indexed: 11/19/2023]
Abstract
Ameloblastoma is a benign odontogenic jawbone tumor. The binding of Nerve growth factor (NGF) to receptor tyrosine kinase A (TrkA) promotes cell survival, proliferation, and differentiation via PI3K/AKT and Ras/MAPK signaling. Although the exact cause of ameloblastoma remains unknown, elevated levels of NGF and TrkA expression in ameloblastoma are associated with aggressive tumor behavior and poor patient outcomes. It is previously demonstrated that His 4, Arg 9, and Glu 11 residues of NGF made crucial interactions with the TrkA subunit. The main aim of our present study to develop potential therapeutic strategies by identifying promising peptide candidates. The objectives include starting with a detailed in silico analysis to identify a crucial peptide sequence of NGF that is bound by TrkA, creating a library of novel peptides from the identified peptide sequence through a single-point mutation on interacting residues (His 4, Arg 9, and Glu 11), and selecting the top peptides based on docking score, interactions analysis, and desirable pose analysis. The study ultimately designed a hybrid peptide candidate through the simultaneous and continuous mutation of the top residues, resulting in a peptide that exhibited a more specific interaction with TrkA, blocking the binding site and preventing the interaction between NGF and TrkA.Communicated by Ramaswamy H. Sarma.
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Affiliation(s)
- Moyim Mallick
- Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research (NIPER), Sahibzada Ajit Singh Nagar, India
| | | | - Sonia Kumari
- Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research (NIPER), Sahibzada Ajit Singh Nagar, India
| | - M Elizabeth Sobhia
- Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research (NIPER), Sahibzada Ajit Singh Nagar, India
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Rahman A, Faruk M, Rusdin MHS, Inriany N, Binekada IMC. Hemimaxillectomy defect reconstruction with a maxillary obturator prosthesis using a 3D-model stereolithography: A case report. Int J Surg Case Rep 2023; 108:108431. [PMID: 37354824 PMCID: PMC10382777 DOI: 10.1016/j.ijscr.2023.108431] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/24/2023] [Revised: 06/12/2023] [Accepted: 06/12/2023] [Indexed: 06/26/2023] Open
Abstract
INTRODUCTION Complete or partial resection of the maxilla during tumor surgery causes oronasal defects, leading to oral-maxillofacial dysfunction, for which the obturator prosthesis is an important treatment option. Traditional manufacturing of obturators involves great complexity, often results in impaired function and fit, and is time-consuming. This paper presents a case of using 3D stereolithography as a guide design for defect reconstruction with a maxillary obturator prosthesis. CASE PRESENTATION We report a 14-year-old girl who presented with facial deformities followed by swelling and pain that reached the orbit floor on the left side of her face. A non-contrast head magnetic resonance imaging scan showed a mass in the left maxilla that extended from the buccal mandible to the left side of the oral cavity. An incisional biopsy indicated that the mass was an ameloblastic fibroma. The entire tumor was treated with a hemi-maxillectomy and reconstruction using an obturator. No further complaints or recurrence signs were evident after six months. DISCUSSION Treatment options for ameloblastoma include enucleation and excision. Due to the severity of the lesion in our case, the entire maxilla had to be removed with the tumor, using a hemimaxillectomy with the assistance of a 3D-printed model, and an obturator was used for reconstruction. CONCLUSION Ameloblastoma is a tumor with a high risk of recurrence. This case underwent a complete hemi-maxillectomy with simultaneous repair using a 3D-printed obturator, with satisfactory functional and cosmetic outcomes.
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Affiliation(s)
- Abd Rahman
- Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia.
| | - Muhammad Faruk
- Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia.
| | - M Hidayat Sakti Rusdin
- Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Hasanuddin, Makassar, Indonesia.
| | - Nur Inriany
- Department of Prosthodontic, Faculty of Dentistry, Universitas Hasanuddin, Makassar, Indonesia.
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A 20-year experience of immediate mandibular reconstruction using free fibula osteocutaneous flaps following ameloblastoma resection: Radical resection, outcomes, and recurrence. Arch Plast Surg 2019; 46:426-432. [PMID: 31550747 PMCID: PMC6759441 DOI: 10.5999/aps.2018.01487] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2018] [Accepted: 08/23/2019] [Indexed: 01/08/2023] Open
Abstract
BACKGROUND The mandible is an important structure that is located in the lower third of the face. Large mandibular defects after tumor resection cause loss of its function. This study assessed the outcomes and tumor recurrence after immediate mandibular reconstruction using a free fibula osteocutaneous flap following radical resection of ameloblastoma. METHODS This is a retrospective non-randomized study of outcomes and tumor recurrence of all patients diagnosed with mandibular ameloblastoma from August 1997 until August 2017 (20 years) requiring free fibula osteocutaneous flap reconstruction at a single institution. The patients were identified through an electronic operative database; subsequently, their medical records and photo documentation were retrieved. RESULTS Twenty-seven patients were included in this study. Eighteen patients were male, while nine were female. The majority of the patients (48.1%) were in their third decade of life when they were diagnosed with ameloblastoma. All of them underwent radical resection of the tumor with a surgical margin of 2 cm (hemimandibulectomy in cases with a large tumor) and immediate mandibular reconstruction with a free fibula osteocutaneous flap. Two patients required revision of a vascular anastomosis due to venous thrombosis postoperatively, while one patient developed a flap recipient site infection. The flap success rate was 100%. There was no tumor recurrence during a mean follow-up period of 5.6 years. CONCLUSIONS Mandibular ameloblastoma should be treated with segmental mandibulectomy (with a surgical margin of 2 cm) to reduce the risk of recurrence. Subsequent mandibular and adjacent soft tissue defects should be reconstructed immediately with a free fibula osteocutaneous flap.
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Kim JY, Kim J, Bazarsad S, Cha IH, Cho SW, Kim J. Bcl-2 is a prognostic marker and its silencing inhibits recurrence in ameloblastomas. Oral Dis 2019; 25:1158-1168. [PMID: 30801855 DOI: 10.1111/odi.13070] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/01/2018] [Revised: 02/12/2019] [Accepted: 02/15/2019] [Indexed: 01/29/2023]
Abstract
OBJECTIVES Ameloblastomas are the most common odontogenic epithelial tumors with high recurrence rate. The aim of this study was to identify apoptosis-related genes with recurrence of ameloblastomas and to evaluate its feasibility as a prognostic marker and as a target molecule preventing from recurrence. MATERIALS AND METHODS Public microarray data were analyzed. To evaluate their expression in ameloblastoma patients, immunohistochemical staining was performed in 89 human ameloblastoma tissues. Quantitative PCR was performed by use of ameloblastoma cell line (AM-1). Fluorescence activated cell sorting analysis and western blotting were conducted following transfection with siRNA. Further, AM-1 cells were implanted in the renal subcapsular layer of immunodeficient mice. RESULTS Microarray data analysis revealed that osteoprotegerin (OPG) and B-cell lymphoma 2 (Bcl-2) were the two most upregulated genes in ameloblastoma. Only Bcl-2 expression was significantly (p = 0.020) associated with recurrence in conservative treatment group (n = 17) among 89 patients. Silencing of Bcl-2 increased apoptosis in AM-1 cells in vitro and inhibited tumor nodule formation of AM-1 cells in vivo. CONCLUSION These results suggest that Bcl-2 expression is a useful biomarker to predict recurrence of ameloblastomas, and as a therapeutic target molecule to prevent recurrence of ameloblastoma.
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Affiliation(s)
- Jue Young Kim
- Department of Oral Pathology, Oral Cancer Research Institute, Seoul, Republic of Korea
| | - Jinsun Kim
- Division of Anatomy and Developmental Biology, Department of Oral Biology, Seoul, Republic of Korea
| | - Shadavlonjid Bazarsad
- Department of Oral Pathology, Oral Cancer Research Institute, Seoul, Republic of Korea
| | - In-Ho Cha
- Department of Oral and Maxillofacial Surgery, Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, Republic of Korea
| | - Sung-Won Cho
- Division of Anatomy and Developmental Biology, Department of Oral Biology, Seoul, Republic of Korea
| | - Jin Kim
- Department of Oral Pathology, Oral Cancer Research Institute, Seoul, Republic of Korea
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Ibikunle AA, Taiwo AO, Braimah RO. A 5-year audit of major maxillofacial surgeries at Usmanu Danfodiyo university teaching hospital, Nigeria. BMC Health Serv Res 2018; 18:416. [PMID: 29879975 PMCID: PMC5992770 DOI: 10.1186/s12913-018-3236-1] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/02/2018] [Accepted: 05/25/2018] [Indexed: 11/10/2022] Open
Abstract
Background There is a paucity of data on the pattern of oral and maxillofacial surgeries done in Nigeria. Despite the rising prominence of oral and maxillofacial surgery as a specialty in our immediate environment, no published audit of the surgeries performed exist. This study aims to present the pattern and types of major surgeries done by oral and maxillofacial surgeons in our hospital as well as the indications for such surgeries. It is hoped that the findings will assist in formulating informed policies and improving healthcare delivery. Methods A review of hospital records of all patients who had major oral and maxillofacial surgeries at Usmanu Danfodiyo University Teaching Hospital from January, 2013 to August, 2017 was done. Descriptive statistics such biodata, indication for surgery and type of surgery were recorded and analyzed using the IBM SPSS statistics for windows version 20 (Armonk, NY: IBM Corp) software. Results One hundred and forty six individuals who underwent 158 major surgeries under general anaesthesia were included. There were 82 males and 64 females, giving a male/female ratio of 1.3: 1. The ages ranged from 3 months to 81 years [median of 33 years]. Tumours and tumour-like lesions were the major indications for surgery [85 (58.2%)]. The most commonly performed surgery was mandibulectomy [31 (19.6%)], followed by Open Reduction and Internal Fixation (ORIF) [29 (18.4%)]. Conclusion Major oral and maxillofacial surgeries are common in our environment. The frequencies of these surgeries can increase with better healthcare financing and universal availability of health insurance schemes. Efforts aimed at reducing the incidence of tumours should be instituted.
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Affiliation(s)
- Adebayo Aremu Ibikunle
- Department of Dental and Maxillofacial Surgery, Usmanu Danfodiyo University Teaching Hospital Sokoto, PMB, Sokoto, 12003, Nigeria.
| | - Abdurrazaq Olanrewaju Taiwo
- Department of Dental and Maxillofacial Surgery, Usmanu Danfodiyo University Teaching Hospital Sokoto, PMB, Sokoto, 12003, Nigeria
| | - Ramat Oyebunmi Braimah
- Department of Dental and Maxillofacial Surgery, Usmanu Danfodiyo University Teaching Hospital Sokoto, PMB, Sokoto, 12003, Nigeria
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Jahanshahi G, Arzhang E, Derisavy S, Davoodi L, Shakeri S. Granular cell type of ameloblastoma. Dent Res J (Isfahan) 2018; 15:224-227. [PMID: 29922343 PMCID: PMC5958541] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
Ameloblastoma is a locally invasive tumor derived from odontogenic epithelium. An uncommon variant of ameloblastoma is granular cell type, which cannot distinguish from other ameloblastoma subtypes by clinical and radiographic findings alone. Only review of it's microscopic features allows distinction from other subtypes. The purpose of this article is to present a case of granular cell ameloblastoma. This subtype should be distinguished from the other histopathologic subtypes because of it's higher recurrence rate and more aggressive biological behavior. Radiographic and histologic findings as well as treatment are also discussed.
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Affiliation(s)
- Gholamreza Jahanshahi
- Dental Research Center and Department of Oral and Maxillofacial Pathology, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran,Address for correspondence: Dr. Gholamreza Jahanshahi, Department of Oral and Maxillofacial Pathology, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran. E-mail:
| | - Elham Arzhang
- Department of Oral and Maxillofacial Pathology, Dental School, Shahrekord University of Medical Sciences, Shahrekord, Iran
| | - Soheila Derisavy
- Department of Oral and Maxillofacial Pathology, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran
| | - Laleh Davoodi
- Department of Operative School of Dentistry, Shahid Sadoughi Yazd University of Medical Sciences, Yazd, Iran
| | - Salman Shakeri
- Department of Oral and Maxillofacial Surgery, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
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Valkadinov I, Conev N, Dzhenkov D, Donev I. Rare case of ameloblastoma with pulmonary metastases. Intractable Rare Dis Res 2017; 6:211-214. [PMID: 28944145 PMCID: PMC5608933 DOI: 10.5582/irdr.2017.01032] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/05/2022] Open
Abstract
Ameloblastoma is a rare low-grade odontogenic tumor of epithelial origin. The World Health Organization (WHO) has defined malignant ameloblastoma (MA) as a histologically benign-appearing ameloblastoma that has metastasized. Treatment of the primary ameloblastoma usually consists of radical excision of the tumor and adjuvant radiotherapy. Chemotherapy should be used to treat metastases due to its indolent clinical course. Presented here is the case of a 43-year-old woman who was admitted to a hospital in 2006 with a large mass involving the neck and left mandible. The mass had formed over years and had been neglected. The woman was diagnosed with a primary ameloblastoma of the mandible. Surgical resection was performed, followed by adjuvant radiotherapy. In September 2016, she was admitted again, and the findings were consistent with metastases of the previously identified ameloblastoma to the lungs. The patient was evaluated for further chemotherapy with 6 cycles of cisplatin at a dose of 100 mg/m2 on day 1, 5-FU at a dose of 1000 mg/m2/day on day 1-4 (3 wk), and pegylated filgrastim. The current case represents the classical course of a rare disease, which in this instance involved the common presentation of MA. This case is a valid incidence of MA based on the typical histology, findings from a lung biopsy, the immunohistochemical profile of the tumor, the typical clinical features, and a history of a previous primary disease.
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Affiliation(s)
- Ivan Valkadinov
- Clinic of Medical Oncology, UMHAT “St. Marina”, Varna, Bulgaria
| | - Nikolay Conev
- Clinic of Medical Oncology, UMHAT “St. Marina”, Varna, Bulgaria
| | - Dian Dzhenkov
- Centre of Clinical Pathology, UMHAT “St. Marina”, Varna, Bulgaria
| | - Ivan Donev
- Clinic of Medical Oncology, UMHAT “St. Marina”, Varna, Bulgaria
- Address correspondence to: Dr. Ivan Donev, Clinic of Medical Oncology, UMHAT “St. Marina”, 1 “Hristo Smirnenski” Blvd., Varna 9000, Bulgaria. E-mail:
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