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Batista NMG, de Moraes ATL, Balbinot KM, de Souza Neto OR, da Silva Brandão JM, da Silva Kataoka MS, de Melo Alves Júnior S, de Jesus Viana Pinheiro J. Immunohistochemical analysis of ADAMTS-1, versican and pEGFR expressions in periapical granuloma and radicular cyst. BMC Oral Health 2021; 21:102. [PMID: 33676487 PMCID: PMC7937253 DOI: 10.1186/s12903-021-01462-x] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2020] [Accepted: 02/25/2021] [Indexed: 11/21/2022] Open
Abstract
Background ADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated. Therefore, our objective was to verify the expression of ADAMTS-1, versican and pEGFR in Periapical Granuloma (PG) and in the Radicular Cyst (RC) since they are the most common lesions of the periapex.
Methods 25 samples of RC and 10 of PG were used. As a control, 10 samples of inflammatory fibrous hyperplasia (IFH) and 10 of dental follicle (DF) were used. The expression of these proteins was investigated using immunohistochemistry. Results In the epithelium of RC, IFH and DF, the expression of ADAMTS-1 was greater in DF than in RC (p < .001). Versicano showed greater expression in IFH than in RC, DF than in RC (p < .001). pEGFR showed greater expression in IFH and RC than in DF (p < .01 and p < .05, respectively). In connective tissue, ADAMTS-1 expression was greater in PG and RC than in IFH and DF (p < .001). Versicano showed greater expression in PG, RC and IFH compared to DF (p < .001). In pEGFR there was a higher expression in PG when compared to RC, IFH and DF (p < .001). Greater immunostaining occurred in the RC than in the DF (p < .001). Conclusions Our results suggest that the studied proteins may participate in the pathogenesis of PG and RC, through the interaction of these proteins, in the remodeling of the ECM (versican) by ADAMTS-1, producing bioactive fragments, which could activate EGFR, contributing to the formation, growth and maintenance of injuries.
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Affiliation(s)
| | - Antonia Taiane Lopes de Moraes
- Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil
| | - Karolyny Martins Balbinot
- Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil
| | | | - Juliana Melo da Silva Brandão
- Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil
| | - Maria Sueli da Silva Kataoka
- Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil
| | - Sérgio de Melo Alves Júnior
- Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil
| | - João de Jesus Viana Pinheiro
- Federal University of Pará Brazil, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil. .,Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil. .,Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Guamá, Belém, PA, 66075110, Brazil.
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Chacur M, Ibrahim D, Arrebola T, Sanches O, Giuffrida R, Oba E, Ramos A. Avaliação da técnica de coloração AgNOR em testículos de ovinos. ARQ BRAS MED VET ZOO 2015. [DOI: 10.1590/1678-6695] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022] Open
Abstract
A coloração pela prata das regiões organizadoras de nucléolos (NORs) é caracterizada por marcar proteínas ligadas ao ácido ribonucleico ribossômico, avaliando a proliferação em células normais ou neoplásicas. Objetivou-se estudar, em testículos de ovinos obtidos em matadouro, a validade do uso da técnica de coloração pela prata (AgNOR) na identificação das regiões organizadoras de nucléolo (NORs) em células saudáveis da linhagem espermatogênica. Utilizaram-se 43 pares de testículos de ovinos mestiços entre seis e 10 meses de idade. Testes de Wilcoxon e Spearman foram empregados, com nível de 5%. As médias das NORs nas células das gônadas direita e esquerda foram, respectivamente: espermatogônia (8,77±1,14 e 9,04±0,96), espermatócitos (4,99±2,00 e 6,20±2,07; P<0,05), Leydig (8,05±2,82 e 7,89±2,29) e Sertoli (8,07±1,88 e 7,61±2,16; P<0,05). Houve correlação (P<0,05) entre os lados para o número de NORs: espermatócitos x Leydig (0,49); espermatócitos x Sertoli (0,49) e Leydig x Sertoli (0,96). Conclui-se ser válido o emprego da técnica AgNOR para avaliar o potencial proliferativo das células saudáveis em testículos de ovinos com prática execução e baixo custo.
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Affiliation(s)
| | | | | | | | | | - E. Oba
- Universidade Estadual Paulista, Brasil
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