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Fellin CR, Steiner RC, Buchen JT, Anders JJ, Jariwala SH. Photobiomodulation and Vascularization in Conduit-Based Peripheral Nerve Repair: A Narrative Review. Photobiomodul Photomed Laser Surg 2024; 42:1-10. [PMID: 38109199 DOI: 10.1089/photob.2023.0103] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2023] Open
Abstract
Background: Peripheral nerve injuries pose a significant clinical issue for patients, especially in the most severe cases wherein complete transection (neurotmesis) results in total loss of sensory/motor function. Nerve guidance conduits (NGCs) are a common treatment option that protects and guides regenerating axons during recovery. However, treatment outcomes remain limited and often fail to achieve full reinnervation, especially in critically sized defects (>3 cm) where a lack of vascularization leads to neural necrosis. Conclusions: A multitreatment approach is, therefore, necessary to improve the efficacy of NGCs. Stimulating angiogenesis within NGCs can help alleviate oxygen deficiency through rapid inosculation with the host vasculature, whereas photobiomodulation therapy (PBMT) has demonstrated beneficial therapeutic effects on regenerating nerve cells and neovascularization. In this review, we discuss the current trends of NGCs, vascularization, and PBMT as treatments for peripheral nerve neurotmesis and highlight the need for a combinatorial approach to improve functional and clinical outcomes.
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Affiliation(s)
- Christopher R Fellin
- The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA
- The Center for Rehabilitation Sciences Research, Department of Physical Medicine and Rehabilitation, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA
| | - Richard C Steiner
- The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA
- The Center for Rehabilitation Sciences Research, Department of Physical Medicine and Rehabilitation, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA
| | - Jack T Buchen
- The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA
- The Center for Rehabilitation Sciences Research, Department of Physical Medicine and Rehabilitation, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA
| | - Juanita J Anders
- Department of Anatomy, Physiology and Genetics, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA
| | - Shailly H Jariwala
- The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA
- The Center for Rehabilitation Sciences Research, Department of Physical Medicine and Rehabilitation, Uniformed Services University of Health Sciences, Bethesda, Maryland, USA
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Kim DS, Kim M, Seo S, Kim JH. Nature-Inspired Chiral Structures: Fabrication Methods and Multifaceted Applications. Biomimetics (Basel) 2023; 8:527. [PMID: 37999168 PMCID: PMC10669407 DOI: 10.3390/biomimetics8070527] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/22/2023] [Revised: 10/31/2023] [Accepted: 11/03/2023] [Indexed: 11/25/2023] Open
Abstract
Diverse chiral structures observed in nature find applications across various domains, including engineering, chemistry, and medicine. Particularly notable is the optical activity inherent in chiral structures, which has emerged prominently in the field of optics. This phenomenon has led to a wide range of applications, encompassing optical components, catalysts, sensors, and therapeutic interventions. This review summarizes the imitations and applications of naturally occurring chiral structures. Methods for replicating chiral architectures found in nature have evolved with specific research goals. This review primarily focuses on a top-down approach and provides a summary of recent research advancements. In the latter part of this review, we will engage in discussions regarding the diverse array of applications resulting from imitating chiral structures, from the optical activity in photonic crystals to applications spanning light-emitting devices. Furthermore, we will delve into the applications of biorecognition and therapeutic methodologies, comprehensively examining and deliberating upon the multifaceted utility of chiral structures.
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Affiliation(s)
- Da-Seul Kim
- Department of Energy Systems Research, Ajou University, Suwon 16499, Republic of Korea (M.K.)
- Department of Chemical Engineering, Ajou University, Suwon 16499, Republic of Korea
| | - Myounggun Kim
- Department of Energy Systems Research, Ajou University, Suwon 16499, Republic of Korea (M.K.)
- Department of Chemical Engineering, Ajou University, Suwon 16499, Republic of Korea
| | - Soonmin Seo
- Department of Bionano Technology, Gachon University, Seongnam 13120, Republic of Korea
| | - Ju-Hyung Kim
- Department of Energy Systems Research, Ajou University, Suwon 16499, Republic of Korea (M.K.)
- Department of Chemical Engineering, Ajou University, Suwon 16499, Republic of Korea
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Wiegand C, Hipler UC, Elsner P, Tittelbach J. Keratinocyte and Fibroblast Wound Healing In Vitro Is Repressed by Non-Optimal Conditions but the Reparative Potential Can Be Improved by Water-Filtered Infrared A. Biomedicines 2021; 9:biomedicines9121802. [PMID: 34944618 PMCID: PMC8698951 DOI: 10.3390/biomedicines9121802] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/15/2021] [Revised: 11/19/2021] [Accepted: 11/26/2021] [Indexed: 11/16/2022] Open
Abstract
It is a general goal to improve wound healing, especially of chronic wounds. As light therapy has gained increasing attention, the positive influence on healing progression of water-filtered infrared A (wIRA), a special form of thermal radiation, has been investigated and compared to the detrimental effects of UV-B irradiation on wound closure in vitro. Models of keratinocyte and fibroblast scratches help to elucidate effects on epithelial and dermal healing. This study further used the simulation of non-optimal settings such as S. aureus infection, chronic inflammation, and anti-inflammatory conditions to determine how these affect scratch wound progression and whether wIRA treatment can improve healing. Gene expression analysis for cytokines (IL1A, IL6, CXCL8), growth (TGFB1, PDGFC) and transcription factors (NFKB1, TP53), heat shock proteins (HSP90AA1, HSPA1A, HSPD1), keratinocyte desmogleins (DSG1, DSG3), and fibroblast collagen (COL1A1, COL3A1) was performed. Keratinocyte and fibroblast wound healing under non-optimal conditions was found to be distinctly reduced in vitro. wIRA treatment could counteract the inflammatory response in infected keratinocytes as well as under chronic inflammatory conditions by decreasing pro-inflammatory cytokine gene expression and improve wound healing. In contrast, in the anti-inflammatory setting, wIRA radiation could re-initiate the acute inflammatory response necessary after injury to stimulate the regenerative processes and advance scratch closure.
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Le Duff F, Fontas E, Guardoli D, Lacour JP, Passeron T. HeaLED: Assessment of skin healing under light-emitting diode (LED) exposure-A randomized controlled study versus placebo. Lasers Surg Med 2021; 54:342-347. [PMID: 34529859 DOI: 10.1002/lsm.23480] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/20/2021] [Accepted: 09/03/2021] [Indexed: 01/08/2023]
Abstract
BACKGROUND Light-emitting diodes (LEDs) in the visible or near-infrared spectrum have been reported to promote wound healing. However, despite being frequently proposed in daily clinical practice, the efficacy of photobiomodulation treatment after a laser procedure relies on very limited clinical data. OBJECTIVE To compare the relative efficacy of LED versus placebo treatment in decreasing erythema and transepidermal water loss (TEWL) after a fractional CO2 session. METHODS We conducted an open prospective intraindividual randomized controlled study with 10 healthy volunteers. An ablative fractional laser was performed on the seven forearm areas. Three consecutive daily sessions of LED (590, 630, and 850 nm [two tested irradiances each] and placebo) were applied after randomization. Physical measures (colorimetry, TEWL), photography, and clinical evaluation were performed on Days 1, 2, 3, 7, and 21. The main criterion of evaluation was the variation of parameter a* (erythema) at 72 hours for each LED parameter compared to placebo treatment. RESULTS No significant differences in the variation of the parameter a* or any of the other studied parameters were found for the different LEDs compared to the placebo area. CONCLUSION Photobiomodulation failed to improve healing after laser ablation compared to placebo.
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Affiliation(s)
- Florence Le Duff
- Dermatology Department, Archet Hospital-CHU, University Côte d'Azur, Nice, France
| | - Eric Fontas
- Délégation à la Recherche Clinique et à l'innovation (DRCI), Université Côte D'Azur, CHU Nice, Nice, France
| | - Davide Guardoli
- Dermatology Department, Archet Hospital-CHU, University Côte d'Azur, Nice, France
| | - Jean-Philippe Lacour
- Dermatology Department, Archet Hospital-CHU, University Côte d'Azur, Nice, France
| | - Thierry Passeron
- Dermatology Department, Archet Hospital-CHU, University Côte d'Azur, Nice, France.,INSERM U 1065, C3M, University Côte d'Azur, Nice, France
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5
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Glass GE. Photobiomodulation: A review of the molecular evidence for low level light therapy. J Plast Reconstr Aesthet Surg 2020; 74:1050-1060. [PMID: 33436333 DOI: 10.1016/j.bjps.2020.12.059] [Citation(s) in RCA: 35] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/06/2020] [Revised: 11/18/2020] [Accepted: 12/19/2020] [Indexed: 12/22/2022]
Abstract
Light energy is harnessed for therapeutic use in a number of ways, most recently by way of photobiomodulation (PBM). This phenomenon is a cascade of physiological events induced by the nonthermal exposure of tissue to light at the near infrared end of the visible spectrum. Therapeutic PBM has become a highly commercialized interest, marketed for everything from facial rejuvenation to fat loss, and diode-based devices are popular in both the clinic setting and for use at home. The lack of regulatory standards makes it difficult to draw clear conclusions about efficacy and safety but it is crucial that we understand the theoretical basis for PBM, so that we can engage in an honest dialogue with our patients and design better clinical studies to put claims of efficacy to the test. This article presents a summary of the science of PBM and examines the differences between laser light, on which much of the preclinical evidence is based and light from diodes, which are typically used in a clinical setting.
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Affiliation(s)
- Graeme E Glass
- Department of Surgery, Sidra Medicine, Doha, Qatar; Chair, laser safety committee, Sidra Medicine, Doha, Qatar; Weill Cornell Medical College, New York and Qatar.
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Zwolińska J. The I/T curve coefficient for evaluating changes in neuromuscular excitability after polarized light irradiation - a placebo-controlled randomized trial. REHABILITACJA MEDYCZNA 2020. [DOI: 10.5604/01.3001.0014] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
Introduction: PILER light affects the sensory and motor excitability of the tissue, and these changes may depend on the color of the filter used in the irradiations.
Objective: To evaluate changes in neuromuscular excitability after PILER irradiation with different filters. To evaluate the usefulness of the I/T curve coefficient in neuromuscular excitability test.
Material and methods: 60 healthy volunteers assigned to four groups: group v - without filter (n=15), group x - red filter (n=15), group y - blue filter (n=15), group z - placebo (n=15) had biceps brachii irradiated with PILER light. Outcome Measures: I/T curve coefficient for rectangular (■I/T coeff) and triangular (▲I/T coeff) pulses for sensory and motor excitability and the pressure pain threshold (PPT).
Results: ■I/T coeff (p=0.0013) and ▲I/T coeff (p=0.0011) for sensory excitability increased significantly in the irradiated group. ■I/T coeff (p=0.0356) and ▲I/T coeff (p=0.0022) increased significantly after blue light irradiation. A significant increase in the▲I/T coeff (p=0.0439) in motor excitability was observed in the irradiated group. ■I/T coeff (p=0.0309) and ▲I/T coeff (p=0.0064) increased significantly after blue light irradiation.
Conclusion: PILER light may reduce muscle excitability. Using a blue filter may increase the sensory threshold, and myorelaxation. Further experiments are necessary to confirm the usefulness of the I/T curve coefficient.
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Affiliation(s)
- Jolanta Zwolińska
- Institute of Health Sciences, Medical College, University of Rzeszow, Rzeszów, Poland St. Hedvig Clinical Provincial Hospital No. 2, Rzeszów, Poland; Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Rzeszów, Poland
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Tripodi N, Feehan J, Husaric M, Kiatos D, Sidiroglou F, Fraser S, Apostolopoulos V. Good, better, best? The effects of polarization on photobiomodulation therapy. JOURNAL OF BIOPHOTONICS 2020; 13:e201960230. [PMID: 32077232 DOI: 10.1002/jbio.201960230] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/18/2019] [Revised: 02/17/2020] [Accepted: 02/17/2020] [Indexed: 06/10/2023]
Abstract
Photobiomodulation therapy (PBMT) is a widely adopted form of phototherapy used to treat many chronic conditions that effect the population at large. The exact physiological mechanisms of PBMT remain unsolved; however, the prevailing theory centres on changes in mitochondrial function. There are many irradiation parameters to consider when investigating PBMT, one of which is the state of polarization. There is some evidence to show that polarization of red and near-infrared light may promote different and/or increased biological activity when compared to otherwise identical non-polarized light. These enhanced cellular effects may also be present when the polarized light is applied linear to the tissue direction. Herein, we synthesize the current experimental and clinical evidence pertaining to polarized photobiomodulation therapy; ultimately, to better inform future research into this area of phototherapy.
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Affiliation(s)
- Nicholas Tripodi
- Institute for Health and Sport, Victoria University, Melbourne, Australia
- First Year College, Victoria University, Melbourne, Australia
| | - Jack Feehan
- Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia
- Department of Medicine-Western Health, Melbourne Medical School, The University of Melbourne, St. Albans, Australia
| | - Maja Husaric
- Institute for Health and Sport, Victoria University, Melbourne, Australia
- First Year College, Victoria University, Melbourne, Australia
| | - Dimitrios Kiatos
- Institute for Health and Sport, Victoria University, Melbourne, Australia
- Department of Medicine-Western Health, Melbourne Medical School, The University of Melbourne, St. Albans, Australia
- College of Health and Biomedicine, Victoria University, Melbourne, Australia
| | - Fotios Sidiroglou
- First Year College, Victoria University, Melbourne, Australia
- Institute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne, Australia
| | - Sarah Fraser
- Institute for Health and Sport, Victoria University, Melbourne, Australia
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Feehan J, Tripodi N, Fraser S, Mikkelsen K, Thewlis A, Kiatos D, Husaric M, Apostolopoulos V. Polarized light therapy: Shining a light on the mechanism underlying its immunomodulatory effects. JOURNAL OF BIOPHOTONICS 2020; 13:e201960177. [PMID: 31816155 PMCID: PMC7065605 DOI: 10.1002/jbio.201960177] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/06/2019] [Revised: 12/03/2019] [Accepted: 12/05/2019] [Indexed: 06/10/2023]
Abstract
This study investigates the immunomodulatory effects of polychromatic polarized light therapy (PLT) on human monocyte cells. While there is some evidence demonstrating a clinical effect in the treatment of certain conditions, there is little research into its mechanism of action. Herein, U937 monocyte cells were cultured and exposed to PLT. The cells were then analyzed for change in expression of genes and cell surface markers relating to inflammation. It was noted that 6 hours of PLT reduced the expression of the CD14, MHC I and CD11b receptors, and increased the expression of CD86. It was also shown that PLT caused downregulation of the genes IL1B, CCL2, NLRP3 and NOD1, and upregulation of NFKBIA and TLR9. These findings imply that PLT has the capacity for immunomodulation in human immune cells, possibly exerting an anti-inflammatory effect.
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Affiliation(s)
- Jack Feehan
- Osteopathy Group, College of Health and BiomedicineVictoria UniversityVictoriaAustralia
- Australian Institute for Musculoskeletal Science (AIMSS)University of Melbourne and Western HealthSt AlbansVictoriaAustralia
| | - Nicholas Tripodi
- Institute for Health and SportVictoria UniversityVictoriaAustralia
- First Year CollegeVictoria UniversityVictoriaAustralia
| | - Sarah Fraser
- Institute for Health and SportVictoria UniversityVictoriaAustralia
| | | | - April Thewlis
- Osteopathy Group, College of Health and BiomedicineVictoria UniversityVictoriaAustralia
| | - Dimitrios Kiatos
- Osteopathy Group, College of Health and BiomedicineVictoria UniversityVictoriaAustralia
- Institute for Health and SportVictoria UniversityVictoriaAustralia
| | - Maja Husaric
- Institute for Health and SportVictoria UniversityVictoriaAustralia
- First Year CollegeVictoria UniversityVictoriaAustralia
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9
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Therapeutic applications of polarized light: Tissue healing and immunomodulatory effects. Maturitas 2018; 116:11-17. [DOI: 10.1016/j.maturitas.2018.07.009] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/08/2018] [Revised: 07/07/2018] [Accepted: 07/18/2018] [Indexed: 12/26/2022]
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Ma H, Yang JP, Tan RK, Lee HW, Han SK. Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro. ACTA ACUST UNITED AC 2018. [DOI: 10.22467/jwmr.2018.00283] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
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11
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Green LED light has anti-inflammatory effects on burns in rats. Burns 2016; 42:392-6. [DOI: 10.1016/j.burns.2015.07.003] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2015] [Revised: 06/05/2015] [Accepted: 07/09/2015] [Indexed: 12/31/2022]
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Takeuchi N, Takezako N, Shimonishi Y, Usuda S. Effect of high-intensity pulse irradiation with linear polarized near-infrared rays on muscle tone in patients with cerebrovascular disease: a randomized controlled trial. J Phys Ther Sci 2015; 27:3817-23. [PMID: 26834360 PMCID: PMC4713799 DOI: 10.1589/jpts.27.3817] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2015] [Accepted: 09/17/2015] [Indexed: 12/28/2022] Open
Abstract
[Purpose] This study evaluated effects of a high-intensity linear polarized near-infrared ray irradiation for mitigation of muscle hypertonia. [Subjects] The subjects were 20 patients with cerebrovascular disease. [Methods] Subjects were randomly allocated to an intervention or control group. The intervention group received irradiation of the triceps surae. Passive range of motion and passive resistive joint torque of ankle dorsiflexion were measured before and after the intervention in knee extended and flexed positions. [Results] In the knee extended position, the mean changes in passive range of motion were 2.70° and -0.50° in the intervention and control groups, respectively, and the mean changes in passive resistive joint torque were -1.42 and -0.26 N·m in the intervention and control groups, respectively. In the knee flexed position, the mean changes in passive range of motion were 3.70° and 0.70° in the intervention and control groups, respectively, and the mean changes in passive resistive joint torque were -2.38 and -0.31 N·m in the intervention and control groups, respectively. In both knee positions, the mean changes in the two indices were greater in the intervention group than in the control group. [Conclusion] High-intensity linear polarized near-infrared ray irradiation increases passive range of motion and decreases passive resistive joint torque.
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Affiliation(s)
- Nobuyuki Takeuchi
- Department of Physical Therapy, Faculty of Health Care,
Takasaki University of Health and Welfare, Japan
- Department of Rehabilitation, Honjo General Hospital,
Japan
| | - Nobuhiro Takezako
- Department of Rehabilitation, Takasaki University of Health
and Welfare Attached Clinic, Japan
| | | | - Shigeru Usuda
- Gunma University Graduate School of Health Sciences,
Japan
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Chaves MEA, Silva FSD, Soares VPC, Ferreira RAM, Gomes FSL, Andrade RMD, Pinotti M. Evaluation of healing of pressure ulcers through thermography: a preliminary study. ACTA ACUST UNITED AC 2015. [DOI: 10.1590/2446-4740.0571] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
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Chaves MEDA, Araújo ARD, Piancastelli ACC, Pinotti M. Effects of low-power light therapy on wound healing: LASER x LED. An Bras Dermatol 2014; 89:616-23. [PMID: 25054749 PMCID: PMC4148276 DOI: 10.1590/abd1806-4841.20142519] [Citation(s) in RCA: 130] [Impact Index Per Article: 11.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2013] [Accepted: 07/29/2013] [Indexed: 12/28/2022] Open
Abstract
Several studies demonstrate the benefits of low-power light therapy on wound
healing. However, the use of LED as a therapeutic resource remains
controversial. There are questions regarding the equality or not of biological
effects promoted by LED and LASER. One objective of this review was to determine
the biological effects that support the use of LED on wound healing. Another
objective was to identify LED´s parameters for the treatment of wounds. The
biological effects and parameters of LED will be compared to those of LASER.
Literature was obtained from online databases such as Medline, PubMed, Science
Direct and Scielo. The search was restricted to studies published in English and
Portuguese from 1992 to 2012. Sixty-eight studies in vitro and in animals were
analyzed. LED and LASER promote similar biological effects, such as decrease of
inflammatory cells, increased fibroblast proliferation, stimulation of
angiogenesis, granulation tissue formation and increased synthesis of collagen.
The irradiation parameters are also similar between LED and LASER. The
biological effects are dependent on irradiation parameters, mainly wavelength
and dose. This review elucidates the importance of defining parameters for the
use of light devices.
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Affiliation(s)
| | | | | | - Marcos Pinotti
- Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
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Effects of red laser, infrared, photodynamic therapy, and green LED on the healing process of third-degree burns: clinical and histological study in rats. Lasers Med Sci 2014; 30:421-8. [PMID: 25391372 DOI: 10.1007/s10103-014-1687-0] [Citation(s) in RCA: 33] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/28/2014] [Accepted: 10/30/2014] [Indexed: 12/29/2022]
Abstract
The aim of this study was to evaluate the effects of red laser, infrared, photodynamic therapy, and green light-emitting diode (LED) on the healing process of skin burns through clinical and histopathologic analysis in rats. For this, 100 animals were randomly divided into five groups: G1-untreated control (CTR), G2-red laser (LVER), G3-infrared (LINF), G4-photodynamic therapy (PDT), and G5-green LED. Burn was induced on the dorsum of the rat and the treatment of the experimental groups was red light (10 J/cm(2), 10 s, 40 mW, and λ660 nm), infrared (10 J/cm(2), 10 s, 40 mW, and λ780 nm), green LED irradiation (60 J/cm(2), 10 s, λ520, and 550 nm), and photodynamic therapy (10 J/cm(2), 40 mW, and λ660 nm), the latter combined with methylene blue photosensitizer at concentration 0.5 μg/mL. Applications were performed daily until day prior to sacrifice of the animal at 3, 7, 14, and 21 days with intraperitoneal anesthetic overdose. The specimens collected were clinically examined and soon after processed and stained with hematoxylin-eosin and Picrosirius for analysis under light and polarized light microscopy, respectively. Animals treated with LVER, LINF, PDT (p < 0.001), and LED (p < 0.05) stimulated production and maturation of collagen, and increased the consumption of food and water compared to the CTR (p < 0.001). Laser λ660 nm and λ780 nm showed the largest wound reductions in all groups (p = 0.001). In conclusion, red laser, infrared, photodynamic therapy, and green LED favored the healing process of third-degree burns in rats.
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Kim DH, Choi JE, Ryu HJ, Seo SH, Kye YC, Ahn HH. Analytic parallel-polarized light imaging technique using various light-emitting diodes: a comparison with skin conductance values. Skin Res Technol 2014; 21:158-63. [DOI: 10.1111/srt.12172] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 05/16/2014] [Indexed: 11/30/2022]
Affiliation(s)
- D. H. Kim
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
| | - J. E. Choi
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
| | - H. J. Ryu
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
| | - S. H. Seo
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
| | - Y. C. Kye
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
| | - H. H. Ahn
- Department of Dermatology; Korea University College of Medicine; Seoul Korea
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17
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Dermaplaning, topical oxygen, and photodynamic therapy: a systematic review of the literature. Aesthetic Plast Surg 2011; 35:1151-9. [PMID: 21533984 DOI: 10.1007/s00266-011-9730-z] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/23/2010] [Accepted: 02/28/2011] [Indexed: 10/18/2022]
Abstract
BACKGROUND Noninvasive procedures for facial rejuvenation are becoming an increasingly popular component of a comprehensive skin care regimen. Concurrently, many new treatment methods are now available to both the plastic surgeon and the aesthetician. Because these techniques have become an integral part of many cosmetic practices, this study aimed to assess the existing evidence-based literature as to their clinical efficacy; to provide an objective overview of some of the most popular noninvasive rejuvenation strategies such as dermaplaning, oxygen therapy, and light therapy; to discuss recent pertinent scientific evidence-based literature; and to provide treatment recommendations based on these findings. METHODS A systematic review was performed in August 2009 using PubMed and the following keywords: "dermaplaning," "oxygen therapy," and "light therapy." All peer-reviewed articles then were screened independently by three plastic surgeons. RESULTS The search identified 42 English-written, peer-reviewed manuscripts. The overall amount of scientific data supporting these methods was found to be scarce, anecdotal, and not well documented. Nevertheless, all three noninvasive therapies have become increasingly popular in the cosmetic market because many patients and physicians or surgeons report being pleased with their results. CONCLUSION Although the evidence supporting these nonsurgical methods is suboptimal, their uses continue to expand. As with any plastic surgery procedure, providing patients with realistic expectations is essential to achieving optimal outcomes and patient satisfaction. Therefore, critical investigation is warranted. In addition, these methods are most effective when included within a comprehensive skin care regimen consisting of sunscreen, vitamin therapy, and lifestyle modification.
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Falus A, Fenyo M, Eder K, Madarasi A. [Polarized light as an epigenetic factor in inhibition of inflammation; a genome-wide expression analysis in recurrent respiratory diseases of children]. Orv Hetil 2011; 152:1492-9. [PMID: 21893480 DOI: 10.1556/oh.2011.29162] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/19/2022]
Abstract
UNLABELLED Whole-body polarized light therapy has been primarily investigated in various clinical observations and in a few in vitro model systems. AIMS In the present study, clinical and molecular effects of whole-body polarized light treatment on children suffering from recurrent respiratory infection were studied. METHODS Incidence and duration of respiratory symptoms as well as the length of appropriate antibiotic therapy have been measured. Simultaneously, genome-wide gene expression pattern was examined by whole genome cDNA microarray in peripheral lymphocytes of children. RESULTS Twenty of twenty five children showed a marked clinical improvement, while in five of twenty five had poor or no changes. Gene expression pattern of the peripheral lymphocytes of the patients was compared in favorable and poor responders. Lymphocytes of the children with a documented improved clinical response to polarized light therapy showed a decrease in the expression of chemokine genes, such as CXCL1, CXCL2, IL-8 and in that of the tumor necrosis alpha (TNFα) gene. On the contrary, a rapid elevation was found in the expression of gene encoding for CYP4F2, a leukotriene-B(4)-metabolizing enzyme. In children with poor clinical response to polarized light therapy, no similar changes were detected in the gene expression pattern of the lymphocytes. CONCLUSIONS Improved clinical symptoms and modified gene expression profile of lymphocytes reveals anti-inflammatory effect upon whole body polarized light irradiation.
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Affiliation(s)
- András Falus
- Semmelweis Egyetem, Általános Orvostudományi Kar Genetikai, Sejt- és Immunbiológiai Intézet Budapest.
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Oliveira PC, Pinheiro ALB, de Castro ICV, Reis JA, Noia MP, Gurgel C, Teixeira Cangussú MC, Pedreira Ramalho LM. Evaluation of the effects of polarized light (λ400-200 nm) on the healing of third-degree burns in induced diabetic and nondiabetic rats. Photomed Laser Surg 2011; 29:619-25. [PMID: 21631376 DOI: 10.1089/pho.2010.2914] [Citation(s) in RCA: 13] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022] Open
Abstract
OBJECTIVE The study evaluated, by light microscopy, the repair process on third-degree burns on diabetic and nondiabetic rats, illuminated or not, with a polarized light (PL) source. BACKGROUND DATA Burns are severe injuries that result in the loss of fluid and destruction of tissue, infection, and shock that may result in death. Diabetes mellitus is a public health problem that, being uncontrolled, causes severe disturbance to the body metabolism, including on wound healing. PL sources have been shown to be effective in improving healing in many situations. MATERIALS AND METHODS Ninety male Wistar rats were divided into two groups (n=45): nondiabetic and diabetic. In one of the groups, diabetes mellitus was induced by streptozotocin. A third-degree burn, measuring 1.5×1.5 cm(2), was created in the dorsum of each animal. Phototherapy (λ400-2000 nm, 10.2 or 20.4 J/cm(2)) started immediately after burning and was repeated daily until animal death (7, 14, and 21 days). Specimens were taken, processed, and stained with H&E and Sirius red and immunomarked with cytokeratin (CK) AE1/AE3. Descriptive analysis was performed by light microscopy. RESULTS Animals subjected to phototherapy showed an acceleration of the repair, the dose of 10.2 J/cm(2) being the one that caused best results, including higher deposition of collagen, quicker inflammatory reaction, and improved revascularization. CONCLUSIONS Our results suggest that the use of PL (10.2 J/cm(2)) improves the healing of third-degree burns on both diabetic and nondiabetic animals.
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Affiliation(s)
- Priscila Chagas Oliveira
- Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil
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Falus A, Fenyo M, Éder K, Madarasi A. Genome-wide gene expression study indicates the anti-inflammatory effect of polarized light in recurrent childhood respiratory disease. Inflamm Res 2011; 60:965-72. [PMID: 21744267 DOI: 10.1007/s00011-011-0357-y] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2011] [Revised: 06/16/2011] [Accepted: 06/20/2011] [Indexed: 11/28/2022] Open
Abstract
OBJECTIVES The clinical and molecular effects of whole-body polarized light treatment on children suffering from recurrent respiratory infection were studied. METHODS The incidence and duration of respiratory symptoms as well as the length of appropriate antibiotic therapy were measured. Simultaneously, the genome-wide gene expression pattern was examined by whole genome cDNA microarray in peripheral lymphocytes of children. RESULTS Twenty of 25 children showed a marked clinical improvement, while in five of 25 had poor response or no changes. The gene expression pattern of the patients' peripheral lymphocytes was compared in favorable and poor responders. The lymphocytes of the children with a documented improved clinical response to polarized light therapy showed a decrease in the expression of chemokine genes, such as CXCL1, CXCL2, CXCL3, and IL-8, and in that of the TNFα gene. On the contrary, a rapid elevation was found in the expression of the gene encoding for CYP4F2, a leukotriene B4-metabolizing enzyme. In children with poor clinical response to polarized light therapy, no similar changes were detected in the gene expression pattern of the lymphocytes. CONCLUSIONS The improved clinical symptoms and modified gene expression profile of lymphocytes reveals an anti-inflammatory effect of whole-body polarized light irradiation.
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Affiliation(s)
- A Falus
- Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary.
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Jin G, Prabhakaran MP, Liao S, Ramakrishna S. Photosensitive materials and potential of photocurrent mediated tissue regeneration. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2010; 102:93-101. [PMID: 20951603 DOI: 10.1016/j.jphotobiol.2010.09.010] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/04/2010] [Accepted: 09/22/2010] [Indexed: 10/19/2022]
Abstract
Photocurrent therapy with participation of light and electrical stimulations could be an innovative and promising approach in regenerative medicine, especially for skin and nerve regeneration. Photocurrent is generated when light irradiates on a photosensitive device, and with more and more types of photosensitive materials being synthesized, photocurrent could be applied for enhanced regeneration of tissue. Photosensitive scaffolds such as composite poly (3-hexylthiophene)/polycaprolactone (P3HT/PCL) nanofibers are fabricated by electrospinning process in our lab for skin regeneration in presence of applied photocurrent. This review article discuss on the various in vitro, in vivo and clinical studies that utilized the principle of 'electrotherapy' and 'phototherapy' for regenerative medicine and evaluates the potential application of photocurrent in regenerative medicine. We conclude that photocurrent therapy will play an important role in regenerative medicine.
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Affiliation(s)
- Guorui Jin
- Department of Mechanical Engineering, National University of Singapore, Singapore, Singapore
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Activation of the extracellular signal-regulated kinase signal pathway by light emitting diode irradiation. Lasers Med Sci 2010; 25:531-7. [PMID: 20162318 DOI: 10.1007/s10103-009-0743-7] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/30/2009] [Accepted: 11/17/2009] [Indexed: 10/19/2022]
Abstract
Irradiation by light emitting diode (LED) promotes fibroblast proliferation and wound healing. However, its mechanism is still unknown. The purpose of this study was to clarify the mechanism of fibroblast proliferation by LED irradiation. Cultured NIH3T3 fibroblasts from normal mice were irradiated by LED with a center wavelength of 627 nm. LED irradiation was performed with an energy density of 4 J/cm(2), at subculture and 24 h later. The expression of several growth factors and their receptors was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR): platelet-derived growth factor (PDGF)-A, PDGF-B, and PDGF-C, transforming growth factor-beta (TGF-beta), basic fibroblast growth factor (bFGF), PDGF-alpha receptor, and TGF-beta receptor. Then, the activation of the extracellular signal-regulated kinase (ERK) pathway was examined by Western blotting with and without the PDGF receptor inhibitor. LED irradiation induced cell growth of NIH3T3 fibroblasts. The expression of PDGF-C had significantly increased in the irradiated group (P < 0.01). Although strong activation of the ERK pathway was observed in the irradiated group, its activation was completely suppressed by the PDGF receptor inhibitor. We concluded that LED irradiation promotes fibroblast proliferation by increasing autocrine production of PDGF-C and activating the ERK pathway through phosphorylation of the PDGF receptor.
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