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Chen B, Liu Y, Liu Y, Xu S. Distribution characteristics of pathogens in different stages of pressure ulcers and the therapeutic effect of linear polarized polychromatic light combined with silver sulfadiazine cream. Medicine (Baltimore) 2023; 102:e35772. [PMID: 37861479 PMCID: PMC10589588 DOI: 10.1097/md.0000000000035772] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/11/2023] [Accepted: 10/03/2023] [Indexed: 10/21/2023] Open
Abstract
To investigate the distribution characteristics of pathogens in different stages of pressure ulcers and observe the application of linear polarized polychromatic light (LPPL) combined with silver sulfadiazine cream in treating varying stages of pressure ulcers. This study comprised 88 patients with pressure ulcers who were enrolled in the department of burn and plastic surgery of our hospital from April 2019 to April 2022. The wound exudates from patients were collected, followed by analyzing the distribution of pathogens in different stages of pressure ulcers. Patients were randomly divided into 2 groups. The first group (n = 44) received LPPL combined with silver sulfadiazine. The other group was intervened with LPPL group only for 2 weeks. The clinical efficacy, condition, and pain in the 2 groups, as well as the healing timeframes for patients were measured at different stages. The findings showed that among 88 patients with pressure ulcers, 62 were infected, and the infection rate was 70.45%. The pathogens that were observed in stage II and III to IV pressure ulcers were mainly Gram-negative bacteria. The total effective rate in the combined group was 90.91%, which was much higher than that of LPPL group (70.45%). Compared with LPPL group, the pressure ulcer scale for healing and visual analogue scale scores in the combined group were markedly lower (P < .05). It is important to note that in LPPL group, the healing time of patients in stage II and stage III to IV in the combined arm were 9.76 ± 2.38 days and 13.19 ± 2.54 days, respectively. The corresponding time in the LPPL group was prolonged to 13.20 ± 3.76 and 18.82 ± 4.17 days, respectively. The main pathogens associated with wound infection in patients with pressure ulcers are Gram-negative bacteria. The curative effects of LPPL combined with sulfadiazine silver cream on patients with pressure ulcer is obviously improved, and the recovery and pain relief are faster while the healing time of pressure ulcer is shorter.
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Affiliation(s)
- Binxiong Chen
- Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong, China
| | - Yang Liu
- Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong, China
| | - Yueming Liu
- Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong, China
| | - Shi Xu
- Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong, China
- Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, Guangdong, China
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Zaki Ewiss MA, Mahmoud MA, Steiner R. Effect of femtosecond laser interaction with human fibroblasts: a preliminary study. Lasers Med Sci 2023; 38:83. [PMID: 36867297 PMCID: PMC9984333 DOI: 10.1007/s10103-023-03740-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/23/2022] [Accepted: 02/18/2023] [Indexed: 03/04/2023]
Abstract
In in vitro methods and cell culture models, femtosecond (fs) laser interaction has been employed to assess its effect on the proliferation and morphology of human skin fibroblasts. We cultured a primary human skin fibroblast cell line on a glass plate, passages 17-23. The cells were irradiated with a 90-fs laser at a wavelength of 800 nm and a repetition rate of 82 MHz. The target received an average power of 320 mW for 5, 20, and 100 s, corresponding to the radiation exposures of 22.6, 90.6, and 452.9 J/cm2, respectively. Using a laser scanning microscopy technique, the photon densities were measured to be 6.4 × 1018, 2.6 × 1019, and 1.3 × 1020 photons/cm2 in a spot area of 0.07 cm2; the recorded spectra were obtained from the laser interaction after 0.00, 1.00, 25.00, and 45.00 h. The cell count and morphological changes showed that the cultured cells were affected by laser irradiation under photon stress; some fibroblasts were killed, while others were injured and survived. We discovered evidence of the formation of several coenzyme compounds, such as flavin (500-600 nm), lipopigments (600-750 nm), and porphyrin (500-700 nm). This study is motivated by the future development of a novel, ultra-short fs laser system and the need to develop a basic in vitro understanding of photon-human cell interaction. The cell proliferation indicated that cells are partly killed or wounded. The exposure of fibroblasts to fs laser fluence up to 450 J/cm2 accelerates cell growth of the viable residual cell.
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Affiliation(s)
- M A Zaki Ewiss
- Department of Physics, Faculty of Science, Cairo University, Giza, 12630, Egypt.
| | - M A Mahmoud
- Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt
| | - R Steiner
- Institute of Laser Technologies in Medicine and Metrology at the University of Ulm, 89081, Ulm, Germany
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Efficacy of Low-Level Laser Therapy in a Rabbit Model of Rhinosinusitis. Int J Mol Sci 2023; 24:ijms24010760. [PMID: 36614203 PMCID: PMC9820841 DOI: 10.3390/ijms24010760] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/18/2022] [Revised: 12/12/2022] [Accepted: 12/16/2022] [Indexed: 01/04/2023] Open
Abstract
Little is known about alternative treatment options for rhinosinusitis (RS). We aimed to evaluate the efficacy of low-level laser therapy (LLLT) for RS in experimentally induced rabbit models of RS. A total of 18 rabbits were divided into four groups: a negative control group (n = 3), an RS group without treatment (n = 5, positive control group), an RS group with natural recovery (n = 5, natural recovery group), and an RS group with laser irradiation (n = 5, laser-treated group). Computed tomography and histopathological staining were performed for each group. mRNA and protein expression levels of local cytokines (IFN-γ, IL-17, and IL-5) were also measured. Tissue inflammation revealed a significant improvement in the laser-treated group compared with the RS and natural recovery groups (p < 0.01). In addition, sinus opacification in the CT scans and cytokine expression was reduced in the laser-treated group, though without statistical significance. LLLT could be an effective option for the management of RS concerning radiological, histological, and molecular parameters.
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Tripodi N, Sidiroglou F, Fraser S, Husaric M, Kiatos D, Apostolopoulos V, Feehan J. The effects of polarized photobiomodulation on cellular viability, proliferation, mitochondrial membrane potential and apoptosis in human fibroblasts: Potential applications to wound healing. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY. B, BIOLOGY 2022; 236:112574. [PMID: 36179581 DOI: 10.1016/j.jphotobiol.2022.112574] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/07/2022] [Revised: 09/05/2022] [Accepted: 09/20/2022] [Indexed: 06/16/2023]
Abstract
Photobiomodulation (PBM) is a widely used therapeutic intervention used to treat several chronic conditions. Despite this, fundamental research underpinning its effectiveness is lacking, highlighted by the lack of a definitive mechanism of action. Additionally, there are many treatment variables which remain underexplored, one of those being the effect of polarization the property of light that specifies the direction of the oscillating electric field. When applied to PBM, using linearly polarized light, when compared to otherwise identical non-polarized light, may enhance its biological efficacy. As such, we investigated the potential biological effects of polarized PBM when compared to non-polarized and non-irradiated controls in the domains of cellular viability, proliferation, apoptosis and mitochondrial membrane potential (ΔΨ) within cells exposed to oxidative stress. It was noted that polarized PBM, when compared to non-polarized PBM and non-irradiated controls, demonstrated mostly increased levels of cellular proliferation and ΔΨ, whilst decreasing the amount of cellular apoptosis. These results indicate that polarization may have utility in the clinical application of PBM. Future research is needed to further elucidate the underpinning mechanisms of PBM and polarization.
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Affiliation(s)
- Nicholas Tripodi
- Institute for Health and Sport, Victoria University, Melbourne, Australia; First Year College, Victoria University, Melbourne, Australia; Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia
| | - Fotios Sidiroglou
- First Year College, Victoria University, Melbourne, Australia; Institute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne, Australia
| | - Sarah Fraser
- Institute for Health and Sport, Victoria University, Melbourne, Australia
| | - Maja Husaric
- Institute for Health and Sport, Victoria University, Melbourne, Australia; First Year College, Victoria University, Melbourne, Australia
| | - Dimitrios Kiatos
- College of Health and Biomedicine, Victoria University, Melbourne, Australia
| | - Vasso Apostolopoulos
- Institute for Health and Sport, Victoria University, Melbourne, Australia; Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia
| | - Jack Feehan
- Institute for Health and Sport, Victoria University, Melbourne, Australia; Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, Australia; Department of Medicine-Western Health, Melbourne Medical School, The University of Melbourne, St. Albans, Australia.
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Low-level laser therapy (LLLT) improves alveolar bone healing in rats. Lasers Med Sci 2021; 37:961-969. [PMID: 34002343 DOI: 10.1007/s10103-021-03340-y] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/30/2020] [Accepted: 05/09/2021] [Indexed: 10/21/2022]
Abstract
The main objective of the present study was to evaluate the effect of low-level laser therapy (LLLT) in enhancing bone healing in irradiated alveolus post-tooth extraction. Sixty male Wistar rats (180 ± 10 g) were used in the present study. The left maxillary first molars were extracted, and the alveolar region was irradiated by diode laser device (GaAlAs) immediately after extraction and for more 3-day daily applications. The animals were randomly assigned into two groups: control group (n = 30, with left maxillary molar extraction-CG) and experimental group (n = 30, with tooth extraction and low-level laser therapy applied to the dental alveolus for 42 s-EG). These groups were divided into subgroups (five rats per subgroup) according to the observation time point-1, 2, 3, 5, 7, and 10 days-post-tooth extraction. The maxillary bone was separated, and the specimens were stained with hematoxylin and eosin, Masson's trichrome, and picrosirius red and immunohistochemistry for RUNX-2. Parametric and nonparametric tests were used with a significance level of 5%. LLLT accelerated bone healing with mature collagen fiber bundles and early new bone formation. Histomorphometric analysis revealed an increase of osteoblast (RUNX-2) and osteoclast (TRAP) activity and in the area percentage of cancellous bone in the lased alveolus compared to the control group. This increase was statistically significant (p < 0.05). Application of LLLT with a GaAlAs diode laser device enhanced bone healing and mineralization on alveolar region.
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Mohamed Abdelgawad L, Abd El-hamed MM, Sabry D, Abdelgwad M. Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway. Rep Biochem Mol Biol 2021; 10:30-40. [PMID: 34277866 PMCID: PMC8279709 DOI: 10.52547/rbmb.10.1.30] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2020] [Accepted: 08/29/2020] [Indexed: 12/11/2022]
Abstract
BACKGROUND Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium. METHODS HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of Nrf2, Keap1, PIK3, and HO-1 and the effect of PBMT on Keap1/Nrf2/Ho-1 Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation. RESULTS Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of Nrf2, Keap1, PIK3, and HO-1 levels (p< 0.05). CONCLUSION photobiomodulation with 3 J/cm2 combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.
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Affiliation(s)
- Latifa Mohamed Abdelgawad
- Department of Medical Laser applications, National Institute of laser enhanced science, Cairo University, Egypt.
| | - Manar Mohy Abd El-hamed
- Department of Medical Laser application, National Institute of laser enhanced science, Cairo University, Egypt.
| | - Dina Sabry
- Department of biochemistry & molecular biology, faculty of medicine, Cairo University, Egypt.
| | - Marwa Abdelgwad
- Department of biochemistry & molecular biology, faculty of medicine, Cairo University, Egypt.
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Silveira GRC, de Lima DC, Cintra LTÂ, Brigagão MRPL, Ervolino E, Fernandes LA. Influence of Doxycycline and InGaAlP Diode Laser at 660 nm Wavelength in the Treatment of Periodontitis Induced in Rats: In Vivo Study. Photochem Photobiol 2021; 97:1104-1115. [PMID: 33713346 DOI: 10.1111/php.13414] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/14/2020] [Revised: 03/05/2021] [Accepted: 03/06/2021] [Indexed: 11/28/2022]
Abstract
This study evaluated the influence of Doxycycline (DOX) and Low-Intensity Laser (LIL) (InGaAlP diode laser) as scaling and root planing (SRP) adjuvants in the treatment of periodontitis induced in rats. The rats received periodontal disease induction, and after 7 days, the ligature was removed, and the animals were divided into groups/treatments: NT-receive no treatment; SRP-submitted only to SRP; DOX-submitted to SRP and DOX irrigation; LIL-submitted to SRP and LIL irradiation; and DOX + LIL-submitted to SRP treatments, DOX irrigation and LIL irradiation. The animals were sacrificed at 7, 15 and 30 days, and then, the analyses were performed. A lower concentration of Alpha-glycoprotein acid and Complement 3 was observed in the DOX + LIL group compared to all the other groups in all the periods, and for Complement 4 at 15 and 30 days (P < 0.01). A lower bone loss (BL) was observed in the DOX + LIL group compared to all the other groups in all the periods (P < 0.01). It can be concluded that LIL was effective in the reduction of proteins, and its association with DOX was effective in the reduction of proteins and BL, in the treatment of periodontal induction in rats.
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Affiliation(s)
| | - Daniela Coelho de Lima
- Faculty of Dentistry, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais, Brazil
| | - Luciano Tavares Ângelo Cintra
- Faculty of Dentistry, Department of Restorative Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil
| | | | - Edílson Ervolino
- Faculty of Dentistry, Department of Basic Sciences, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil
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Photobiomodulation and Oxidative Stress: 980 nm Diode Laser Light Regulates Mitochondrial Activity and Reactive Oxygen Species Production. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2021; 2021:6626286. [PMID: 33763170 PMCID: PMC7952159 DOI: 10.1155/2021/6626286] [Citation(s) in RCA: 44] [Impact Index Per Article: 11.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/23/2020] [Revised: 02/09/2021] [Accepted: 02/17/2021] [Indexed: 12/20/2022]
Abstract
Photobiomodulation with 808 nm laser light electively stimulates Complexes III and IV of the mitochondrial respiratory chain, while Complexes I and II are not affected. At the wavelength of 1064 nm, Complexes I, III, and IV are excited, while Complex II and some mitochondrial matrix enzymes seem to be not receptive to photons at that wavelength. Complex IV was also activated by 633 nm. The mechanism of action of wavelengths in the range 900–1000 nm on mitochondria is less understood or not described. Oxidative stress from reactive oxygen species (ROS) generated by mitochondrial activity is an inescapable consequence of aerobic metabolism. The antioxidant enzyme system for ROS scavenging can keep them under control. However, alterations in mitochondrial activity can cause an increment of ROS production. ROS and ATP can play a role in cell death, cell proliferation, and cell cycle arrest. In our work, bovine liver isolated mitochondria were irradiated for 60 sec, in continuous wave mode with 980 nm and powers from 0.1 to 1.4 W (0.1 W increment at every step) to generate energies from 6 to 84 J, fluences from 7.7 to 107.7 J/cm2, power densities from 0.13 to 1.79 W/cm2, and spot size 0.78 cm2. The control was equal to 0 W. The activity of the mitochondria's complexes, Krebs cycle enzymes, ATP production, oxygen consumption, generation of ROS, and oxidative stress were detected. Lower powers (0.1–0.2 W) showed an inhibitory effect; those that were intermediate (0.3–0.7 W) did not display an effect, and the higher powers (0.8–1.1 W) induced an increment of ATP synthesis. Increasing the power (1.2–1.4 W) recovered the ATP production to the control level. The interaction occurred on Complexes III and IV, as well as ATP production and oxygen consumption. Results showed that 0.1 W uncoupled the respiratory chain and induced higher oxidative stress and drastic inhibition of ATP production. Conversely, 0.8 W kept mitochondria coupled and induced an increase of ATP production by increments of Complex III and IV activities. An augmentation of oxidative stress was also observed, probably as a consequence of the increased oxygen consumption and mitochondrial isolation experimental conditions. No effect was observed using 0.5 W, and no effect was observed on the enzymes of the Krebs cycle.
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The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2021; 214:112100. [DOI: 10.1016/j.jphotobiol.2020.112100] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/23/2020] [Revised: 10/28/2020] [Accepted: 11/30/2020] [Indexed: 12/16/2022]
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Varma SR, AlShayeb M, Narayanan J, Abuhijleh E, Hadi A, Jaber M, Abu Fanas S. Applications of Lasers in Refractory Periodontitis: A Narrative Review. J Int Soc Prev Community Dent 2020; 10:384-393. [PMID: 33042878 PMCID: PMC7523935 DOI: 10.4103/jispcd.jispcd_241_20] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/29/2020] [Revised: 05/15/2020] [Accepted: 05/22/2020] [Indexed: 01/17/2023] Open
Abstract
OBJECTIVE The purpose of the study is to evaluate the various lasers in dentistry and to investigate if it can be used for treatment of refractory periodontitis. METHODS The study followed partially the PRISMA guidelines as it is a narrative review. A number of articles were selected from a period of 1980 to 2020 from databases, PubMed, PubMed central, Cochrane and Scopus. Articles related to the effects of lasers on periodontitis both refractory and aggressive were investigated. RESULTS After reviewing the literature, 70 articles were found, related to application of lasers in periodontal diseases. Out of the 70, 11 articles pertained to the effect of laser for the treatment of Refractory and inflammatory periodontitis. 5 articles related to experimental animal models, one pertaining to in-vitro and six studies related to in-vivo in human cohorts. DISCUSSION It was found that lasers if used in controlled parameters by incorporating laser assisted treatment such as Photodynamic therapy and low level laser therapy can be of use as an adjunct therapy for treatment of refractory periodontitis. The use of different wavelengths in the initial and maintenance phase of periodontal disease plays a positive role. The presence of in-vitro and animal model studies is one of the limitation to this study. The available studies have shown marked reduction in inflammation and better clinical and microbiological parameters. The drawback of this study is the limited literature involving laser management for refractory periodontitis in human cohorts. CONCLUSION Different wavelengths of laser and choice of laser assisted periodontal treatment plays an important role in the overall progress and prognosis of periodontal disease activity.
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Affiliation(s)
- Sudhir Rama Varma
- Department of Periodontics, College of Dentistry, Ajman University, Ajman, UAE
| | - Maher AlShayeb
- Department of Oral Surgery, College of Dentistry, Ajman University, Ajman, UAE
| | - Jayaraj Narayanan
- Department of Basic Sciences, University of Science and Technology of Fujairah, Fujairah, UAE
| | - Eyas Abuhijleh
- Department of Orthodontics, University of Science and Technology, Fujairah, UAE
| | - Abdul Hadi
- Department of Restorative Dentistry, University of Science and Technology, Fujairah, UAE
| | - Mohammad Jaber
- Department of Oral Surgery, College of Dentistry, Ajman University, Ajman, UAE
| | - Salim Abu Fanas
- Department of Periodontics, College of Dentistry, Ajman University, Ajman, UAE
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Ferrando S, Agas D, Mirata S, Signore A, De Angelis N, Ravera S, Utyuzh AS, Parker S, Sabbieti MG, Benedicenti S, Amaroli A. The 808 nm and 980 nm infrared laser irradiation affects spore germination and stored calcium homeostasis: A comparative study using delivery hand-pieces with standard (Gaussian) or flat-top profile. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2019; 199:111627. [PMID: 31536925 DOI: 10.1016/j.jphotobiol.2019.111627] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/27/2019] [Revised: 08/20/2019] [Accepted: 09/10/2019] [Indexed: 01/21/2023]
Abstract
Photobiomodulation relies on the transfer of energy from incident photons to a cell photoacceptor. For many years the concept of photobiomodulation and its outcome has been based upon a belief that the sole receptor within the cell was the mitochondrion. Recently, it has become apparent that there are other photoacceptors operating in different regions of the electromagnetic spectrum. Alternative photoacceptors would appear to be water and mechanisms regulating calcium homeostasis, despite a direct effect of laser photonic energy on intracellular calcium concentration outwith mitochondrial activity or influence, have not been clearly demonstrated. Therefore, to increase the knowledge of intracellular‑calcium and laser photon interaction, as well as to demonstrate differences in irradiation profiles with modern hand-pieces, we tested and compared the photobiomodulatory effect of 808 nm and 980 nm diode laser light by low- and higher-energy (60s, 100 mW/cm2, 100 mW/cm2, 500 mW/cm2, 1000 mW/cm2, 1500 mW/cm2, 2000 mW/cm2) irradiated with a "standard" (Gaussian fluence distribution) hand-piece or with a "flat-top" (uniform fluence) hand-piece. For this purpose, we used the eukaryote unicellular-model Dictyostelium discoideum. The 808 nm and 980 nm infrared laser light, at the energy tested directly affect the stored Ca2+ homeostasis, independent of the mitochondrial respiratory chain activities. From an organism perspective, the effect on Ca2+-dependent signal transduction as the regulator of spore germination in Dictyostelium, demonstrates how a cell can respond quickly to the correct laser photonic stimulus through a different cellular pathway than the known light-chromophore(mitochondria) interaction. Additionally, both hand-piece designs tested were able to photobiomodulate the D. discoideum cell; however, the hand-piece with a flat-top profile, through uniform fluence levels allows more effective and reproducible effects.
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Affiliation(s)
- Sara Ferrando
- Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genova, Genova, Italy
| | - Dimitrios Agas
- School of Biosciences and Veterinary Medicine, University of Camerino, Camerino,Macerata, Italy
| | - Serena Mirata
- Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genova, Genova, Italy
| | - Antonio Signore
- Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C), University of Genova, Genova, Italy; Faculty of Therapeutic Stomatology, Institute of Dentistry, I. M. Sechenov First Moscow State Medical University, Moscow, Russia
| | - Nicola De Angelis
- Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C), University of Genova, Genova, Italy; University of Technology MARA, Department of Dentistry, Sungai Buloh, Malaysia
| | - Silvia Ravera
- Department of Experimental Medicine, University of Genova, Genova, Italy
| | - Anatoliy S Utyuzh
- Department of Orthopaedic Dentistry, Sechenov First Moscow State Medical University, Trubetzkaya St., 8, Bd. 2, 119991 Moscow, Russian Federation
| | - Steven Parker
- Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C), University of Genova, Genova, Italy
| | - Maria Giovanna Sabbieti
- School of Biosciences and Veterinary Medicine, University of Camerino, Camerino,Macerata, Italy
| | - Stefano Benedicenti
- Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C), University of Genova, Genova, Italy
| | - Andrea Amaroli
- Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C), University of Genova, Genova, Italy; Department of Orthopaedic Dentistry, Sechenov First Moscow State Medical University, Trubetzkaya St., 8, Bd. 2, 119991 Moscow, Russian Federation.
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Habashneh RA, Mashal MA, Khader Y, Qudah R. Clinical and Biological Effects of Adjunctive Photodynamic Therapy in Refractory Periodontitis. J Lasers Med Sci 2019; 10:139-145. [PMID: 31360383 PMCID: PMC6499576 DOI: 10.15171/jlms.2019.22] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/24/2022]
Abstract
Introduction: To date, no novel treatment approach is available for optimum outcomes regarding refractory periodontitis. The aim of the present study was to assess the efficiency of photodynamic therapy (PDT) in treating patients diagnosed with refractory periodontitis and compare the clinical and biological outcomes of conventional periodontal treatment with or without adjunctive PDT in these patients, by assessing clinical parameters (plaque index [PI], gingival recession [GR], bleeding on probing [BOP], periodontal probing depth [PPD] and clinical attachment level [CAL]) as well as biological parameters (IL-1β) in the gingival crevicular fluid (GCF). Methods: Sixteen patients within the age of 30 to 60 years, with a mean age of 40 years old, diagnosed with refractory periodontitis were included. In this split mouth design study, 2 quads (1 upper + 1 lower) from the same patient were randomly treated with (scaling and root planing [SRP]+PDT) together. The other 2 quadrants (1 upper + 1 lower) were treated by SRP only and selected to serve as controls. Clinical parameters including PI, GR, BOP, PPD and CAL and biological parameters (IL-1β) in the GCF were measured at baseline, then at, 2 and 6 months after therapy. Results: A statistically significant reduction in several clinical parameters as, BOP (P < 0.001), PI (P < 0.001), PPD (P < 0.001) and CAL (P < 0.001) in quadrant treated with SRP and adjunctive PDT when compared to control group treated with SRP alone was observed and both therapies showed non-statistically significant differences in the reduction of IL-1β level. Conclusion: The inclusion of PDT as an adjunctive measure to nonsurgical conventional periodontal treatment seems to be a useful therapeutic measure in refractory periodontitis treatment.
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Affiliation(s)
- Rola Al Habashneh
- Department of Preventive Dentistry, Jordan University of Science and Technology, Ramtha, Jordan
| | - Mohammad A Mashal
- Department of Preventive Dentistry, Jordan University of Science and Technology, Ramtha, Jordan
| | - Yousef Khader
- Medical Education and Biostatistics, Department of Community Medicine, Public Health and Family Medicine, Faculty of Medicine, Jordan University of Science & Technology, Ramtha, Jordan
| | - Rana Qudah
- Department of Preventive Dentistry, Jordan University of Science and Technology, Ramtha, Jordan
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13
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Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results. Lasers Med Sci 2019; 34:1253-1260. [PMID: 30737587 DOI: 10.1007/s10103-019-02733-4] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/22/2017] [Accepted: 01/21/2019] [Indexed: 12/15/2022]
Abstract
The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment, n = 5), SRPG (SRP, n = 10), aPDTW (SRP+aPDT-MB/water, n = 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose, n = 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (p < 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm2) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (p > 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.
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14
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Efficacy of Biophysical Energies on Healing of Diabetic Skin Wounds in Cell Studies and Animal Experimental Models: A Systematic Review. Int J Mol Sci 2019; 20:ijms20020368. [PMID: 30654555 PMCID: PMC6359711 DOI: 10.3390/ijms20020368] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/18/2018] [Revised: 01/04/2019] [Accepted: 01/14/2019] [Indexed: 12/22/2022] Open
Abstract
We have systematically assessed published cell studies and animal experimental reports on the efficacy of selected biophysical energies (BPEs) in the treatment of diabetic foot ulcers. These BPEs include electrical stimulation (ES), pulsed electromagnetic field (PEMF), extracorporeal shockwave (ECSW), photo energies and ultrasound (US). Databases searched included CINAHL, MEDLINE and PubMed from 1966 to 2018. Studies reviewed include animal and cell studies on treatment with BPEs compared with sham, control or other BPEs. Information regarding the objective measures of tissue healing and data was extracted. Eighty-two studies were eventually selected for the critical appraisal: five on PEMF, four each on ES and ECSW, sixty-six for photo energies, and three about US. Based on the percentage of original wound size affected by the BPEs, both PEMF and low-level laser therapy (LLL) demonstrated a significant clinical benefit compared to the control or sham treatment, whereas the effect of US did not reveal a significance. Our results indicate potential benefits of selected BPEs in diabetic wound management. However, due to the heterogeneity of the current clinical trials, comprehensive studies using well-designed trials are warranted to confirm the results.
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15
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Evaluation of scars in children after treatment with low-level laser. Lasers Med Sci 2018; 33:1991-1995. [DOI: 10.1007/s10103-018-2572-z] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/17/2018] [Accepted: 06/22/2018] [Indexed: 10/28/2022]
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16
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Houreld NN, Ayuk SM, Abrahamse H. Cell Adhesion Molecules are Mediated by Photobiomodulation at 660 nm in Diabetic Wounded Fibroblast Cells. Cells 2018; 7:cells7040030. [PMID: 29659538 PMCID: PMC5946107 DOI: 10.3390/cells7040030] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/09/2018] [Revised: 04/09/2018] [Accepted: 04/12/2018] [Indexed: 12/13/2022] Open
Abstract
Diabetes affects extracellular matrix (ECM) metabolism, contributing to delayed wound healing and lower limb amputation. Application of light (photobiomodulation, PBM) has been shown to improve wound healing. This study aimed to evaluate the influence of PBM on cell adhesion molecules (CAMs) in diabetic wound healing. Isolated human skin fibroblasts were grouped into a diabetic wounded model. A diode laser at 660 nm with a fluence of 5 J/cm2 was used for irradiation and cells were analysed 48 h post-irradiation. Controls consisted of sham-irradiated (0 J/cm2) cells. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of CAM-related genes. Ten genes were up-regulated in diabetic wounded cells, while 25 genes were down-regulated. Genes were related to transmembrane molecules, cell–cell adhesion, and cell–matrix adhesion, and also included genes related to other CAM molecules. PBM at 660 nm modulated gene expression of various CAMs contributing to the increased healing seen in clinical practice. There is a need for new therapies to improve diabetic wound healing. The application of PBM alongside other clinical therapies may be very beneficial in treatment.
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Affiliation(s)
- Nicolette N Houreld
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.
| | - Sandra M Ayuk
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.
| | - Heidi Abrahamse
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.
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17
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George S, Hamblin MR, Abrahamse H. Current and Future Trends in Adipose Stem Cell Differentiation into Neuroglia. Photomed Laser Surg 2018; 36:230-240. [PMID: 29570423 DOI: 10.1089/pho.2017.4411] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/15/2023] Open
Abstract
BACKGROUND Neurological diseases and disorders pose a challenge for treatment and rehabilitation due to the limited capacity of the nervous system to repair itself. Adipose stem cells (ASCs) are more pliable than any adult stem cells and are capable of differentiating into non-mesodermal tissues, including neurons. Transdifferentiating ASCs to specific neuronal lineage cells enables us to deliver the right type of cells required for a replacement therapy into the nervous system. METHODS Several methodologies are being explored and tested to differentiate ASCs to functional neurons and glia with cellular factors and chemical compounds. However, none of these processes and prototypes has been wholly successful in changing the cellular structure and functional status of ASCs to become identical to neuroglial cells. In addition, successful integration and functional competence of these cells for use in clinical applications remain problematic. Photobiomodulation or low-level laser irradiation has been successfully applied to not only improve ASC viability and proliferation but has also shown promise as a possible enhancer of ASC differentiation. CONCLUSIONS Studies have shown that photobiomodulation improves the use of stem cell transplantation for neurological applications. This review investigates current neuro-differentiation inducers and suitable methodologies, including photobiomodulation, utilizing ASCs for induction of differentiation into neuronal lineages.
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Affiliation(s)
- Sajan George
- 1 Laser Research Centre, Faculty of Health Sciences, University of Johannesburg , Doornfontein, South Africa
| | - Michael R Hamblin
- 2 Wellman Centre for Photomedicine, Massachusetts General Hospital , Boston, Massachusetts.,3 Department of Dermatology, Harvard Medical School , Boston, Massachusetts.,4 Harvard-MIT Division of Health Sciences and Technology , Cambridge, Massachusetts
| | - Heidi Abrahamse
- 1 Laser Research Centre, Faculty of Health Sciences, University of Johannesburg , Doornfontein, South Africa
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18
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Djavid GE, Bigdeli B, Goliaei B, Nikoofar A, Hamblin MR. Photobiomodulation leads to enhanced radiosensitivity through induction of apoptosis and autophagy in human cervical cancer cells. JOURNAL OF BIOPHOTONICS 2017; 10:1732-1742. [PMID: 28464474 PMCID: PMC5668202 DOI: 10.1002/jbio.201700004] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/07/2017] [Revised: 02/05/2017] [Accepted: 02/15/2017] [Indexed: 06/07/2023]
Abstract
The radiomodulatory effect of photobiomodulation (PBM) has recently been studied in cancer cells. The aim of this study was to investigate cellular mechanisms involved in the X-ray radiosensitivity of HeLa cells pre-exposed to PBM. HeLa cells were irradiated with 685 nm laser at different energy densities prior to X-ray ionizing radiation. After irradiation, clonogenic cell survival, cell death due to apoptosis and autophagy were determined. Levels of intracellular reactive oxygen species (ROS), DNA damage and, cell cycle distribution after PBM were measured. PBM at different energy densities (5-20 J/cm2 ) was not cytotoxic. However, HeLa cells pre-exposed to 20 J/cm2 showed enhanced inhibition of colony formation following ionizing radiation. Enhanced radiosensitivity was due to increased oxidative stress, DNA damage, and radiation-induced apoptosis and autophagy. These results suggest that 685 nm PBM at a higher energy density could possibly be a promising radiosensitizing agent in cervical cancer, to decrease the radiation dose delivered, and therefore prevent the side-effects that are associated with cancer radiotherapy.
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Affiliation(s)
- Gholamreza Esmaeeli Djavid
- Medical Laser Research Center, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran
| | - Bahareh Bigdeli
- Laboratory of Biophysics and Molecular Biology, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
| | - Bahram Goliaei
- Laboratory of Biophysics and Molecular Biology, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
| | - Alireza Nikoofar
- Radiotherapy Department, Firoozgar Hospital, Iran University of Medical sciences. Tehran, Iran
| | - Michael R Hamblin
- Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA
- Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USAHarvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, USA
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19
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The JAK/STAT signaling pathway and photobiomodulation in chronic wound healing. Cytokine Growth Factor Rev 2017; 38:73-79. [DOI: 10.1016/j.cytogfr.2017.10.001] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/05/2017] [Revised: 09/29/2017] [Accepted: 10/02/2017] [Indexed: 12/26/2022]
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Lugongolo MY, Manoto SL, Ombinda-Lemboumba S, Maaza M, Mthunzi-Kufa P. The effects of low level laser therapy on both HIV-1 infected and uninfected TZM-bl cells. JOURNAL OF BIOPHOTONICS 2017; 10:1335-1344. [PMID: 28128530 DOI: 10.1002/jbio.201600217] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/05/2016] [Revised: 12/14/2016] [Accepted: 12/15/2016] [Indexed: 06/06/2023]
Abstract
Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, the quest for new therapies continues. Low level laser therapy (LLLT) involves the exposure of cells to low levels of red or infrared light. LLLT has been widely used in different medical conditions, but not in HIV-1 infection. This study aimed to determine the effects of LLLT on HIV-1 infected and uninfected TZM-bl cells. Both infected and uninfected cells were irradiated at a wavelength of 660 nm with different fluences from 2 J/cm2 to 10 J/cm2 . Changes in cellular responses were assessed using cell morphology, viability, proliferation, cytotoxicity and luciferase activity assays. Upon data analysis, uninfected irradiated cells showed no changes in cell morphology, viability, proliferation and cytotoxicity, while the infected irradiated cells did. In addition, laser irradiation reduced luciferase activity in infected cells. Finally, laser irradiation had no inhibitory effect in uninfected cells, whereas it induced cell damage in a dose dependent manner in infected cells.
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Affiliation(s)
- Masixole Yvonne Lugongolo
- Council of Scientific and Industrial Research, National Laser Centre, P.O. Box 395, Building 46A, Pretoria, 0001, South Africa
- Department of Physics, University of South Africa, Science Campus, Florida, 1710, South Africa
| | - Sello Lebohang Manoto
- Council of Scientific and Industrial Research, National Laser Centre, P.O. Box 395, Building 46A, Pretoria, 0001, South Africa
| | - Saturnin Ombinda-Lemboumba
- Council of Scientific and Industrial Research, National Laser Centre, P.O. Box 395, Building 46A, Pretoria, 0001, South Africa
| | - Malik Maaza
- Department of Physics, University of South Africa, Science Campus, Florida, 1710, South Africa
| | - Patience Mthunzi-Kufa
- Council of Scientific and Industrial Research, National Laser Centre, P.O. Box 395, Building 46A, Pretoria, 0001, South Africa
- Department of Physics, University of South Africa, Science Campus, Florida, 1710, South Africa
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21
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Eissa M, Salih WHM. The influence of low-intensity He-Ne laser on the wound healing in diabetic rats. Lasers Med Sci 2017; 32:1261-1267. [PMID: 28547073 DOI: 10.1007/s10103-017-2230-x] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2015] [Accepted: 05/12/2017] [Indexed: 10/19/2022]
Abstract
The low-level laser irradiation at certain wavelengths is reported to facilitate the healing process of diabetic wounds. Thus, this study carried out to look for the suitable laser parameters that could speed up the healing process. Fourteen healthy male and female rats were used in which a circular wound with a diameter of 2.5 ± 0.2 cm was created on the dorsum in each rat after injected them with alloxan to induced diabetic. They have been divided into two groups: control group (N = 7) and study group (N = 7) to conduct the study. He-Ne laser with a wavelength of 632.8 nm at power density of 4.0 mW/cm2 was used to irradiate the study group for five times a week until the wound healed (closed) completely, while the control group was kept untreated. The results showed that the laser-treated group healed (wounds were totally closed) faster compared to the control group. In numbers, the laser-treated group healed on average at the 21st day (0.0 ± 0.0 cm) (P ≤ 0.005), whereas the control group healed after 40 days or even 60 days in some cases (sample no. 2). This confirms that laser promotes the tissue repair process of diabetic wounds and reduces the healing period to the half.
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Affiliation(s)
- Maha Eissa
- Department of Laser, Alneelain University, Khartoum, Sudan
| | - Wasil H M Salih
- Department of Biomedical Physics, Alneelain University, Khartoum, Sudan.
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22
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de Almeida JM, de Moraes RO, Gusman DJR, Faleiros PL, Nagata MJH, Garcia VG, Theodoro LH, Bosco AF. Influence of low-level laser therapy on the healing process of autogenous bone block grafts in the jaws of systemically nicotine-modified rats: A histomorphometric study. Arch Oral Biol 2017; 75:21-30. [DOI: 10.1016/j.archoralbio.2016.12.003] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2016] [Revised: 10/21/2016] [Accepted: 12/01/2016] [Indexed: 10/20/2022]
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Goymen M, Isman E, Taner L, Kurkcu M. Histomorphometric evaluation of the effects of various diode lasers and force levels on orthodontic mini screw stability. Photomed Laser Surg 2016; 33:29-34. [PMID: 25594769 DOI: 10.1089/pho.2014.3833] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
OBJECTIVE The purpose of this study was to evaluate the effects of different laser dose and force levels on the stability of orthodontic mini screws used for anchorage, by histomorphometric analyses. BACKGROUND DATA Low-level laser therapy speeds up blood flow, improves the mechanism of the revitalization processes, reduces the risk of infection, boosts metabolic activities, and accelerates the healing of the damaged tissue. Although there are many research studies about low-level laser therapy applications in a variety of areas, no investigations were found concerning mini screw stability using various laser dose levels with different force level applications. METHODS Seventeen New Zealand white rabbits were used. A total of 68 cylindrical, self-drilling orthodontic mini screws were threaded at the fibula. Experimental subjects were divided into six groups; force application was not performed in the first three groups, whereas 150 g of force was applied via nickel-titanium closed-coil springs placed between two mini screws in the other three groups. Measurements of the initial torque values (10 Ncm) were manipulated by a digital portable torque gauge. Various low-level laser doses were applied to the groups during the postoperative 10 days. After 4 weeks, bone-to-implant contact and cortical bone thickness were histomorphometrically analyzed. RESULTS In the 150 g force plus 20 J/cm(2) dosage group, the highest bone-to-implant contact values were observed. (p<0.05) There were no statistically significant correlations between cortical bone thickness and bone-to-implant contact values; on the other hand, no significant difference was found among the same groups in terms of cortical bone thickness values (p>0.05). CONCLUSIONS Low-level laser therapy was noticed to induce the mini screw-bone contact area. Low-level laser therapy may be a supplementary treatment method to increase the stability of the orthodontic mini screw.
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Affiliation(s)
- Merve Goymen
- 1 Department of Orthodontics, Faculty of Dentistry, Gaziantep University , Gaziantep, Turkey
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An 808-nm Diode Laser with a Flat-Top Handpiece Positively Photobiomodulates Mitochondria Activities. Photomed Laser Surg 2016; 34:564-571. [PMID: 27622977 DOI: 10.1089/pho.2015.4035] [Citation(s) in RCA: 45] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023] Open
Abstract
OBJECTIVE Photobiomodulation is proposed as a non-linear process. Only the action of light at a low intensity and fluence is assumed to have stimulation on cells; whereas a higher light intensity and fluence generates negative effects, exhausting the cell's energy reserve as a consequence of a too strong stimulation. In our work, we detected the photobiomodulatory effect of an 808-nm higher-fluence diode laser [64 J/cm2-1 W, continuous wave (CW)] irradiated by a flat-top handpiece on mitochondria activities, such as oxygen consumption, activity of mitochondria complexes I, II, III, and IV, and cytochrome c as well as ATP synthesis. MATERIALS AND METHODS The experiments are performed by standard procedure on mitochondria purified from bovine liver. RESULTS Our higher-fluence diode laser positively photobiomodulates the mitochondria oxygen consumption, the activity of the complexes III and IV, and the ATP production, with a P/O = 2.6. The other activities are not influenced. CONCLUSION Our data show for the first time that even the higher fluences (64 J/cm2-1 W), similar to the low fluences, can photobiostimulate the mitochondria respiratory chain without uncoupling them and can induce an increment in the ATP production. These results suggest that the negative effects of higher fluences observed to date are not unequivocally due to higher fluence per se but might be a consequence of the irradiation carried by handpieces with a Gaussian profile.
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25
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Hendudari F, Piryaei A, Hassani SN, Darbandi H, Bayat M. Combined effects of low-level laser therapy and human bone marrow mesenchymal stem cell conditioned medium on viability of human dermal fibroblasts cultured in a high-glucose medium. Lasers Med Sci 2016; 31:749-757. [PMID: 26984346 DOI: 10.1007/s10103-016-1867-1] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/17/2015] [Accepted: 01/03/2016] [Indexed: 02/07/2023]
Abstract
Low-level laser therapy (LLLT) exhibited biostimulatory effects on fibroblasts viability. Secretomes can be administered to culture mediums by using bone marrow mesenchymal stem cells conditioned medium (BM-MSCs CM). This study investigated the combined effects of LLLT and human bone marrow mesenchymal stem cell conditioned medium (hBM-MSCs CM) on the cellular viability of human dermal fibroblasts (HDFs), which was cultured in a high-glucose (HG) concentration medium. The HDFs were cultured either in a concentration of physiologic (normal) glucose (NG; 5.5 mM/l) or in HG media (15 mM/l) for 4 days. LLLT was performed with a continuous-wave helium-neon laser (632.8 nm, power density of 0.00185 W/cm(2) and energy densities of 0.5, 1, and 2 J/cm(2)). About 10% of hBM-MSCs CM was added to the HG HDF culture medium. The viability of HDFs was evaluated using dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. A significantly higher cell viability was observed when laser of either 0.5 or 1 J/cm(2) was used to treat HG HDFs, compared to the control groups. The cellular viability of HG-treated HDFs was significantly lower compared to the LLLT + HG HDFs, hBM-MSCs CM-treated HG HDFs, and LLLT + hBM-MSCs CM-treated HG HDFs. In conclusion, hBM-MSCs CM or LLLT alone increased the survival of HG HDFs cells. However, the combination of hBM-MSCs CM and LLLT improved these results in comparison to the conditioned medium.
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Affiliation(s)
- Farzane Hendudari
- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran
| | - Abbas Piryaei
- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran.
- Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| | - Seyedeh-Nafiseh Hassani
- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
| | - Hasan Darbandi
- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mohammad Bayat
- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, 19395/4719, Tehran, Iran.
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26
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Kikuchi T, Mogi M, Okabe I, Okada K, Goto H, Sasaki Y, Fujimura T, Fukuda M, Mitani A. Adjunctive Application of Antimicrobial Photodynamic Therapy in Nonsurgical Periodontal Treatment: A Review of Literature. Int J Mol Sci 2015; 16:24111-26. [PMID: 26473843 PMCID: PMC4632741 DOI: 10.3390/ijms161024111] [Citation(s) in RCA: 55] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/30/2015] [Revised: 09/26/2015] [Accepted: 10/08/2015] [Indexed: 12/14/2022] Open
Abstract
Periodontal disease is caused by dental plaque biofilms, and the removal of these biofilms from the root surface of teeth plays a central part in its treatment. The conventional treatment for periodontal disease fails to remove periodontal infection in a subset of cases, such as those with complicated root morphology. Adjunctive antimicrobial photodynamic therapy (aPDT) has been proposed as an additional treatment for this infectious disease. Many periodontal pathogenic bacteria are susceptible to low-power lasers in the presence of dyes, such as methylene blue, toluidine blue O, malachite green, and indocyanine green. aPDT uses these light-activated photosensitizer that is incorporated selectively by bacteria and absorbs a low-power laser/light with an appropriate wavelength to induce singlet oxygen and free radicals, which are toxic to bacteria. While this technique has been evaluated by many clinical studies, some systematic reviews and meta-analyses have reported controversial results about the benefits of aPDT for periodontal treatment. In the light of these previous reports, the aim of this review is to provide comprehensive information about aPDT and help extend knowledge of advanced laser therapy.
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Affiliation(s)
- Takeshi Kikuchi
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Makio Mogi
- Department of Medicinal Biochemistry, School of Pharmacy, Aichi Gakuin University, Nagoya, Aichi 464-8650, Japan.
| | - Iichiro Okabe
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Kosuke Okada
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Hisashi Goto
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Yasuyuki Sasaki
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Takeki Fujimura
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Mitsuo Fukuda
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
| | - Akio Mitani
- Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
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Kurach LM, Stanley BJ, Gazzola KM, Fritz MC, Steficek BA, Hauptman JG, Seymour KJ. The Effect of Low-Level Laser Therapy on the Healing of Open Wounds in Dogs. Vet Surg 2015; 44:988-96. [DOI: 10.1111/vsu.12407] [Citation(s) in RCA: 32] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
Affiliation(s)
| | | | | | | | - Barbara A. Steficek
- Diagnostic Center for Population and Animal Health; College of Veterinary Medicine; Michigan State University; East Lansing Michigan
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28
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Photobiostimulation effect on diabetic wound at different power density of near infrared laser. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2015; 151:201-7. [DOI: 10.1016/j.jphotobiol.2015.08.009] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/23/2015] [Revised: 07/30/2015] [Accepted: 08/11/2015] [Indexed: 12/25/2022]
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Low-level laser therapy (LLLT) associated with aerobic plus resistance training to improve inflammatory biomarkers in obese adults. Lasers Med Sci 2015; 30:1553-63. [DOI: 10.1007/s10103-015-1759-9] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/19/2014] [Accepted: 04/23/2015] [Indexed: 02/07/2023]
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30
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Affiliation(s)
- Nicolette N Houreld
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg , Johannesburg, South Africa
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Sergio LPDS, Silva APAD, Amorim PF, Campos VMA, Magalhães LAG, de Paoli F, de Souza da Fonseca A. DNA damage in blood cells exposed to low-level lasers. Lasers Surg Med 2015; 47:361-8. [PMID: 25740459 DOI: 10.1002/lsm.22344] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 01/08/2015] [Indexed: 11/06/2022]
Abstract
BACKGROUND AND OBJECTIVE In regenerative medicine, there are increasing applications of low-level lasers in therapeutic protocols for treatment of diseases in soft and in bone tissues. However, there are doubts about effects on DNA, and an adequate dosimetry could improve the safety of clinical applications of these lasers. This work aimed to evaluate DNA damage in peripheral blood cells of Wistar rats induced by low-level red and infrared lasers at different fluences, powers, and emission modes according to therapeutic protocols. MATERIAL AND METHODS Peripheral blood samples were exposed to lasers and DNA damage was accessed by comet assay. In other experiments, DNA damage was accessed in blood cells by modified comet assay using formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III enzymes. RESULTS Data show that exposure to low-level red and infrared lasers induce DNA damage depending on fluence, power and emission mode, which are targeted by Fpg and endonuclease III. CONCLUSION Oxidative DNA damage should be considered for therapeutic efficacy and patient safety in clinical applications based on low-level red and infrared lasers.
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Affiliation(s)
- Luiz Philippe da Silva Sergio
- Laboratório, de Ciências, Radiológicas, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, 20550900, Brazil
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Amaroli A, Parker S, Dorigo G, Benedicenti A, Benedicenti S. Paramecium: A Promising Non-Animal Bioassay to Study the Effect of 808 nm Infrared Diode Laser Photobiomodulation. Photomed Laser Surg 2015; 33:35-40. [DOI: 10.1089/pho.2014.3829] [Citation(s) in RCA: 22] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Affiliation(s)
- Andrea Amaroli
- Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Genoa, Italy
| | - Steven Parker
- Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, Italy
| | - Gianluca Dorigo
- Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Genoa, Italy
| | - Alberico Benedicenti
- Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, Italy
| | - Stefano Benedicenti
- Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, Italy
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Chaves MEDA, Araújo ARD, Piancastelli ACC, Pinotti M. Effects of low-power light therapy on wound healing: LASER x LED. An Bras Dermatol 2014; 89:616-23. [PMID: 25054749 PMCID: PMC4148276 DOI: 10.1590/abd1806-4841.20142519] [Citation(s) in RCA: 130] [Impact Index Per Article: 11.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2013] [Accepted: 07/29/2013] [Indexed: 12/28/2022] Open
Abstract
Several studies demonstrate the benefits of low-power light therapy on wound
healing. However, the use of LED as a therapeutic resource remains
controversial. There are questions regarding the equality or not of biological
effects promoted by LED and LASER. One objective of this review was to determine
the biological effects that support the use of LED on wound healing. Another
objective was to identify LED´s parameters for the treatment of wounds. The
biological effects and parameters of LED will be compared to those of LASER.
Literature was obtained from online databases such as Medline, PubMed, Science
Direct and Scielo. The search was restricted to studies published in English and
Portuguese from 1992 to 2012. Sixty-eight studies in vitro and in animals were
analyzed. LED and LASER promote similar biological effects, such as decrease of
inflammatory cells, increased fibroblast proliferation, stimulation of
angiogenesis, granulation tissue formation and increased synthesis of collagen.
The irradiation parameters are also similar between LED and LASER. The
biological effects are dependent on irradiation parameters, mainly wavelength
and dose. This review elucidates the importance of defining parameters for the
use of light devices.
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Affiliation(s)
| | | | | | - Marcos Pinotti
- Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
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Passanezi E, Damante CA, de Rezende MLR, Greghi SLA. Lasers in periodontal therapy. Periodontol 2000 2014; 67:268-91. [DOI: 10.1111/prd.12067] [Citation(s) in RCA: 38] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 02/02/2014] [Indexed: 12/12/2022]
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Affiliation(s)
- Timon Cheng-Yi Liu
- Laboratory of Laser Sports Medicine, South China Normal University , Guangzhou 510006, China
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Shedding light on a new treatment for diabetic wound healing: a review on phototherapy. ScientificWorldJournal 2014; 2014:398412. [PMID: 24511283 PMCID: PMC3913345 DOI: 10.1155/2014/398412] [Citation(s) in RCA: 59] [Impact Index Per Article: 5.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2013] [Accepted: 10/09/2013] [Indexed: 12/22/2022] Open
Abstract
Impaired wound healing is a common complication associated with diabetes with complex pathophysiological underlying mechanisms and often necessitates amputation. With the advancement in laser technology, irradiation of these wounds with low-intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in wound healing. At the correct laser parameters, LILI has shown to increase migration, viability, and proliferation of diabetic cells in vitro; there is a stimulatory effect on the mitochondria with a resulting increase in adenosine triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective effect on these cells. In light of the ever present threat of diabetic foot ulcers, infection, and amputation, new improved therapies and the fortification of wound healing research deserves better prioritization. In this review we look at the complications associated with diabetic wound healing and the effect of laser irradiation both in vitro and in vivo in diabetic wound healing.
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Effects of laser irradiation at different wavelengths (660, 810, 980, and 1,064 nm) on mucositis in an animal model of wound healing. Lasers Med Sci 2013; 29:1807-13. [DOI: 10.1007/s10103-013-1336-z] [Citation(s) in RCA: 46] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/18/2013] [Accepted: 04/22/2013] [Indexed: 10/26/2022]
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38
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Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats. Lasers Med Sci 2013; 29:203-12. [DOI: 10.1007/s10103-013-1312-7] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/28/2012] [Accepted: 03/15/2013] [Indexed: 10/27/2022]
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Esmaeelinejad M, Bayat M, Darbandi H, Bayat M, Mosaffa N. The effects of low-level laser irradiation on cellular viability and proliferation of human skin fibroblasts cultured in high glucose mediums. Lasers Med Sci 2013; 29:121-9. [PMID: 23455657 DOI: 10.1007/s10103-013-1289-2] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/25/2011] [Accepted: 02/18/2013] [Indexed: 01/31/2023]
Abstract
Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine. This study has aimed to evaluate the effects of low-level laser therapy (LLLT) on human skin fibroblasts (HSFs) cultured in a high glucose concentration. HSFs were cultured either in a concentration of physiologic glucose (5.5 mM/l) or high glucose media (11.1 and 15 mM/l) for either 1 or 2 weeks after which they were subsequently cultured in either the physiologic glucose or high concentration glucose media during laser irradiation. LLLT was carried out with a helium-neon (He-Ne) laser unit at energy densities of 0.5, 1, and 2 J/cm(2), and power density of 0.66 mW/cm(2) on 3 consecutive days. HSFs' viability and proliferation rate were evaluated with the dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. The LLLT at densities of 0.5 and 1 J/cm(2) had stimulatory effects on the viability and proliferation rate of HSFs cultured in physiologic glucose (5.5 mM/l) medium compared to their control cultures (p = 0.002 and p = 0.046, respectively). All three doses of 0.5, 1, and 2 J/cm(2) had stimulatory effects on the proliferation rate of HSFs cultured in high glucose concentrations when compared to their control cultures (p = 0.042, p = 0.000, and p = 0.000, respectively). This study showed that HSFs originally cultured for 2 weeks in high glucose concentration followed by culture in physiologic glucose during laser irradiation showed enhanced cell viability and proliferation. Thus, LLLT had a stimulatory effect on these HSFs.
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Masha RT, Houreld NN, Abrahamse H. Low-Intensity Laser Irradiation at 660 nm Stimulates Transcription of Genes Involved in the Electron Transport Chain. Photomed Laser Surg 2013; 31:47-53. [DOI: 10.1089/pho.2012.3369] [Citation(s) in RCA: 49] [Impact Index Per Article: 4.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/02/2023] Open
Affiliation(s)
- Roland T. Masha
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, Johannesburg, South Africa
| | - Nicolette N. Houreld
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, Johannesburg, South Africa
| | - Heidi Abrahamse
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, Johannesburg, South Africa
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Ayuk SM, Houreld NN, Abrahamse H. Collagen production in diabetic wounded fibroblasts in response to low-intensity laser irradiation at 660 nm. Diabetes Technol Ther 2012; 14:1110-7. [PMID: 23057714 DOI: 10.1089/dia.2012.0125] [Citation(s) in RCA: 58] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/12/2022]
Abstract
BACKGROUND Collagen type I (Col-I) is a major component of the extracellular matrix and is important in wound healing processes. Several studies have shown that low-intensity laser irradiation (LILI) biostimulates Col-I synthesis both in vitro and in vivo. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model. MATERIALS AND METHODS This study was performed on isolated human skin fibroblasts. Different cell models (normal and diabetic wounded) were used. Cells were irradiated with 5 J/cm(2) at a wavelength of 660 nm and incubated for 48 or 72 h. Nonirradiated cells (0 J/cm(2)) were used as controls. Cellular viability (Trypan blue exclusion test), morphology (bright-field microscopy), proliferation [VisionBlue™ quick cell proliferation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and Col-I (enzyme-linked immunoabsorbent assay) were assessed. RESULTS Diabetic wounded cells irradiated with 5 J/cm(2) at 660 nm showed a significant increase in cell migration, viability, proliferation, and collagen content. CONCLUSIONS This study shows that LILI stimulates Col-I synthesis in diabetic wound healing in vitro at 660 nm.
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Affiliation(s)
- Sandra M Ayuk
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa
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Peccin MS, Renno ACM, de Oliveira F, Giusti PR, Ribeiro DA. Helium-neon laser improves skin repair in rabbits. J COSMET LASER THER 2012; 14:286-9. [PMID: 23057697 DOI: 10.3109/14764172.2012.738917] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
The purpose of this study was to evaluate the influence of helium-neon laser on skin injury in rabbits. For this purpose, 15 New Zealand rabbits underwent bilateral skin damage in leg. Helium-neon laser light, at a fluence of 6 J∕cm2 and wavelength of 632.8 nm, was applied on the left legs (laser group). The right leg lesions (control group) served as negative control. All sections were histopathologically analyzed using HE sections. The results showed little infiltration of inflammatory cells, with proliferation of fibroblasts forming a few fibrous connective tissue after 1 week post-injury. The lesion on the 3rd week was characterized by granulation tissue, which formed from proliferated fibrous connective tissue, congested blood vessels and mild mononuclear cell infiltration. On the 5th week, it was observed that debris material surrounded by a thick layer of connective tissue and dense collage, fibroblasts cells present in the dermis covered by a thick epidermal layer represented by keratinized epithelium. Taken together, our results suggest that helium-neon laser is able to improve skin repair in rabbits at early phases of recovery.
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Affiliation(s)
- Maria Stella Peccin
- Department of Human Movement Sciences, Federal University of São Paulo, UNIFESP, SP, Brazil
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43
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Obradović R, Kesić L, Mihailović D, Jovanović G, Antić S, Brkić Z. Low-level lasers as an adjunct in periodontal therapy in patients with diabetes mellitus. Diabetes Technol Ther 2012; 14:799-803. [PMID: 22928615 PMCID: PMC3429330 DOI: 10.1089/dia.2012.0027] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/02/2023]
Abstract
BACKGROUND Diabetes mellitus (DM) increases the risk of periodontitis, and severe periodontitis often coexists with severe DM. The proposed dual pathway of tissue destruction suggests that control of chronic periodontal infection and gingival inflammation is essential for achieving long-term control of DM. The purpose this study is to evaluate the effects of low-level laser therapy (LLLT) by exfoliative cytology in patients with DM and gingival inflammation. SUBJECTS AND METHODS Three hundred patients were divided in three equal groups: Group 1 consisted of patients with periodontitis and type 1 DM, Group 2 of patients with periodontitis and type 2 DM, and Group 3 of patients with periodontitis (control group). After oral examination, smears were taken from gingival tissue, and afterward all of the patients received oral hygiene instructions, removal of dental plaque, and full-mouth scaling and root planing. A split-mouth design was applied; on the right side of jaws GaAlAs LLLT (670 nm, 5 mW, 14 min/day) (model Mils 94; Optica Laser, Sofia, Bulgaria) was applied for five consecutive days. After the therapy was completed, smears from both sides of jaws were taken. The morphometric analysis was done using the National Institutes of Health Image software program and a model NU2 microscope (Carl Zeiss, Jena, Germany). RESULTS Investigated parameters were significantly lower after therapy compared with values before therapy. After therapy on the side subjected to LLLT, there was no significantly difference between patients with DM and the control group. CONCLUSIONS It can be concluded that LLLT as an adjunct in periodontal therapy reduces gingival inflammation in patients with DM and periodontitis.
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Affiliation(s)
- Radmila Obradović
- Department of Periodontology and Oral Medicine, University of Niš, Niš, Serbia.
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44
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Houreld NN, Masha RT, Abrahamse H. Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells. Lasers Surg Med 2012; 44:429-34. [PMID: 22488690 DOI: 10.1002/lsm.22027] [Citation(s) in RCA: 47] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 03/14/2012] [Indexed: 12/13/2022]
Abstract
BACKGROUND AND OBJECTIVE Low-intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes. MATERIALS AND METHODS Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm(2). Non-irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post-irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively. RESULTS Irradiated diabetic mitochondria at a fluence of 15 J/cm(2) showed a significant decrease in complex III activity (P < 0.05). Normal (P < 0.01) and diabetic (P < 0.05) mitochondria irradiated at either 5 or 15 J/cm(2) showed a significant increase in complex IV activity. ATP results showed a significant increase in irradiated normal cells (5 J/cm(2); P < 0.05) and diabetic cells (15 J/cm(2); P < 0.01). There was a higher accumulation of active mitochondria in irradiated cells than non-irradiated cells. CONCLUSION Irradiation at 660 nm has the ability to influence mitochondrial enzyme activity, in particular cytochrome c oxidase. This leads to increased mitochondrial activity and ATP synthesis.
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Affiliation(s)
- Nicolette N Houreld
- Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa.
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45
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A histological evaluation of a low-level laser therapy as an adjunct to periodontal therapy in patients with diabetes mellitus. Lasers Med Sci 2012; 28:19-24. [DOI: 10.1007/s10103-012-1058-7] [Citation(s) in RCA: 31] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2011] [Accepted: 01/18/2012] [Indexed: 11/28/2022]
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Garcia VG, Fernandes LA, Macarini VC, Almeida JM, Martins TM, Bosco AF, Nagata MJH, Cirelli JA, Theodoro LH. Treatment of experimental periodontal disease with antimicrobial photodynamic therapy in nicotine-modified rats. J Clin Periodontol 2011; 38:1106-14. [DOI: 10.1111/j.1600-051x.2011.01785.x] [Citation(s) in RCA: 28] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 07/23/2011] [Indexed: 10/17/2022]
Affiliation(s)
| | | | - Valmir Campos Macarini
- Group of Research and Study on Laser in Dentistry (GEPLO); Department of Surgery and Integrated Clinic; Division of Periodontics; São Paulo State University; Araçatuba; São Paulo; Brazil
| | - Juliano Milanezi Almeida
- Group of Research and Study on Laser in Dentistry (GEPLO); Department of Surgery and Integrated Clinic; Division of Periodontics; São Paulo State University; Araçatuba; São Paulo; Brazil
| | - Thiago Marchi Martins
- Group of Research and Study on Laser in Dentistry (GEPLO); Department of Surgery and Integrated Clinic; Division of Periodontics; São Paulo State University; Araçatuba; São Paulo; Brazil
| | - Alvaro Francisco Bosco
- Group of Research and Study on Laser in Dentistry (GEPLO); Department of Surgery and Integrated Clinic; Division of Periodontics; São Paulo State University; Araçatuba; São Paulo; Brazil
| | - Maria José Hitomi Nagata
- Group of Research and Study on Laser in Dentistry (GEPLO); Department of Surgery and Integrated Clinic; Division of Periodontics; São Paulo State University; Araçatuba; São Paulo; Brazil
| | - Joni Augusto Cirelli
- Department of Diagnosis and Surgery; Division of Periodontics; São Paulo State University; Araraquara; São Paulo; Brazil
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Garcia VG, Macarini VC, de Almeida JM, Bosco AF, Nagata MJH, Okamoto T, Longo M, Theodoro LH. Influence of low-level laser therapy on wound healing in nicotine-treated animals. Lasers Med Sci 2011; 27:437-43. [DOI: 10.1007/s10103-011-0956-4] [Citation(s) in RCA: 13] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/25/2011] [Accepted: 06/24/2011] [Indexed: 12/17/2022]
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Bottura P, Milanezi J, Fernandes L, Caldas H, Abbud-Filho M, Garcia V, Baptista M. Nonsurgical Periodontal Therapy Combined with Laser and Photodynamic Therapies for Periodontal Disease in Immunosuppressed Rats. Transplant Proc 2011; 43:2009-16. [DOI: 10.1016/j.transproceed.2011.03.083] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2011] [Accepted: 03/29/2011] [Indexed: 11/16/2022]
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Fernandes LA, Martins TM, de Almeida JM, Theodoro LH, Garcia VG. Radiographic assessment of photodynamic therapy as an adjunctive treatment on induced periodontitis in immunosuppressed rats. J Appl Oral Sci 2010; 18:237-43. [PMID: 20857000 PMCID: PMC5349045 DOI: 10.1590/s1678-77572010000300007] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/01/2008] [Accepted: 02/16/2010] [Indexed: 12/13/2022] Open
Abstract
Objective The aim of this study was to assess radiographically the effect of photodynamic
therapy (PDT) as an adjunctive treatment to scaling and root planing (SRP) on
induced periodontitis in dexamethasone-induced immunosuppressed rats. Material and Methods The animals were divided into 2 groups: ND group (n=60): saline treatment; D group
(n=60): dexamethasone treatment. In both ND and D groups, periodontal disease was
induced by the placement of a ligature in the left first mandibular molar. After 7
days, ligature was removed and all animals received SRP, being divided according
to the following treatments: SRP: saline and PDT: phenothiazinium dye (TBO) plus
laser irradiation. Ten animals per treatment were killed at 7, 15 and 30 days. The
distance between the cementoenamel junction and the height of the alveolar bone
crest in the mesial surface of the mandibular left first molars was determined in
millimeters in each radiograph. The radiographic values were analyzed
statistically by ANOVA and Tukey's test at a p value <0.05. Results Intragroup radiographic assessment (ND and D groups) showed that there was
statistically significant less bone loss in the animals treated with PDT in all
experimental periods compared to those submitted to SRP. Intergroup radiographic
analysis (ND and D groups) demonstrated that there was greater bone loss in the ND
group treated with SRP compared to the D group treated with PDT at 7 and 30
days. Conclusion PDT was an effective adjunctive treatment to SRP on induced periodontitis in
dexamethasone-induced immunosuppressed rats.
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Affiliation(s)
- Leandro Araújo Fernandes
- Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil.
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Medeiros JL, Nicolau RA, Nicola EMD, dos Santos JN, Pinheiro ALB. Healing of surgical wounds made with lambda970-nm diode laser associated or not with laser phototherapy (lambda655 nm) or polarized light (lambda400-2000 nm). Photomed Laser Surg 2010; 28:489-96. [PMID: 19857050 DOI: 10.1089/pho.2009.2592] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
OBJECTIVE The aim of this study was to analyze the effect of two phototherapies, laser and polarized light, on diode laser (970lambda nm) wounds. BACKGROUND DATA Lasers have been used in surgery, and some wavelengths may cause thermal damage to the tissue and affect healing. Several studies have shown that some wavelengths are effective in improving healing. Coherent and noncoherent light have been successfully used on the modulation of biological phenomena of several origins. ANIMALS AND METHODS Thirty-one Wistar rats were divided into 3 groups (GI to GIII). A 20-mm x 2-mm wound was created on the dorsum of each animal with a diode laser (Sirolaser, Sirona, Bensheim, Germany). Group GI acted as control. On GII, laser light (lambda655 nm, 30 mW, phi approximately 3 mm, 12 J/cm(2)) was used and on GIII illumination with polarized light (lambda400-2000 nm, 40 mW, phi approximately 5.5 cm, 12 J/cm(2)) was used, every other day (GII) or daily (GIII) for 7 days. The animals were killed at 0, 7, and 14 days after surgery. Specimens were taken, routinely processed, stained and imunnomarked [HE (hematoxylin-eosin), sirius red, alpha-smooth muscle actin (SMA)], and underwent histological analysis. RESULTS GII showed better response at day 14 when re-epithelialization was in a more advanced stage. The number of myofibroblasts was significantly different over the healing time (7 to 14 days); this number was smaller than that observed on G1. On GIII at day 7, the number of myofibroblasts was significantly higher than for GII. At day 14, a more pronounced deposition of collagen matrix was also seen, and inflammation was discrete and more advanced for GIII. CONCLUSION The results of the present study showed that the effect of the use of laser light was more evident at early stages of healing and that the use of polarized light improved the resolution of the inflammatory reaction, increased the deposition of collagen, increased the number of myofibroblasts, and quickened re-epithelialization during the experimental time.
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Affiliation(s)
- Juliana L Medeiros
- Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil
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