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Altom FM, Bedair GY, Eysawi EA, Hammoudah DK, Khoja LA, Yaseen RA, Sabooni GM, Al Qahtani ZA. Evaluation of the Cytological Changes of the Oral Mucosa Among Smokers in Al Madinah Al Munawara Using Argyrophilic Nucleolar Organizer Region (AgNOR) Counts and Papanicolaus Stain. Cureus 2023; 15:e39367. [PMID: 37362451 PMCID: PMC10285572 DOI: 10.7759/cureus.39367] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 05/21/2023] [Indexed: 06/28/2023] Open
Abstract
Objective To evaluate the cytological changes of the oral mucosa among smokers using Argyrophilic nucleolar organizer region (AgNOR) counts and Papanicolaou (Pap) staining. Methodology The oral mucosal exfoliate smears of 500 individuals (200 nonsmokers and 300 smokers) aged between 18 and 80 years were prepared in Al Madinah. The AgNOR count and Pap stain were used to generate a cytogenic smear to assess the presence of cytological changes suggestive of atypia, inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. Results Smokers have a considerably higher number of AgNORs per nucleus than nonsmokers (1.99 3.53 vs. 0.42 1.22). There were inflammatory changes in 127 (42.3%) of the cases and 40 (20%) of the controls. Multinucleated cells and atypia were found in 33 (11%) and 14 (4.5%) of the cases but not in the controls. The results indicate higher proliferative activity in smoking patients compared to nonsmoking patients, even in the absence of clinical lesions. Conclusion To detect the effects of smoking on the oral mucosa, Pap staining alone is insufficient. Combining Pap staining with the AgNOR technique produces the desired results.
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Affiliation(s)
- Faris M Altom
- College of Medicine, Al-Rayan Colleges, Madinah, SAU
| | | | - Eman A Eysawi
- College of Medicine, Al-Rayan Colleges, Madinah, SAU
| | | | - Lina A Khoja
- College of Medicine, Al-Rayan Colleges, Madinah, SAU
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Stähelin H, Francisco ALN, Mariano FV, Kowalski LP, Gondak R. Impact of smoking on dendritic cells in patients with oral squamous cell carcinoma. Braz Oral Res 2021; 35:e075. [PMID: 34495136 DOI: 10.1590/1807-3107bor-2021.vol35.0075] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2020] [Accepted: 12/12/2020] [Indexed: 11/22/2022] Open
Abstract
Smoking has been shown to alter innate and adaptive immune responses and is directly associated with the onset of oral squamous cell carcinoma (OSCC). The purpose of this study was to evaluate the effect of cigarette smoke exposure on dendritic cells (DCs) from OSCC patients. CD1a and CD83 antibodies were used to identify immature and mature DCs, respectively, by immunohistochemistry in OSCC samples of 24 smokers and 24 non-smokers. Density of DCs was calculated in intra and peritumoral areas. Clinical and microscopic findings were reviewed and analyzed for all patients. Smokers with OSCC had a lower density of intra and peritumoral DCs when compared to non-smokers. Tumors classified as moderately/poorly differentiated had lower peritumoral CD1a+ DCs than well-differentiated tumors (p < 0.001). Smoking contributed to a depletion of immature and mature DCs in the OSCC.
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Affiliation(s)
- Heron Stähelin
- Universidade Federal de Santa Catarina - UFSC, Department of Dentistry, Florianópolis, SC, Brazil
| | | | - Fernanda Viviane Mariano
- Universidade Estadual de Campinas - Unicamp, School of Medical Sciences, Department of Pathology, Campinas, SP, Brazil
| | | | - Rogério Gondak
- Universidade Federal de Santa Catarina - UFSC, Department of Pathology, Florianópolis, SC, Brazil
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Vyas T, Verma P, Abidullah M, Kushwaha SS, Sahoo PK, Priyadarshini SR, Subudhi SK, Rana V. Quantitative analysis of AgNOR counts and pap stain in exfoliative cytology specimens of oral mucosa in bidi smokers and nonsmokers. Ann Afr Med 2019; 17:210-214. [PMID: 30588935 PMCID: PMC6330784 DOI: 10.4103/aam.aam_69_17] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
Background: Bidi smoking is a serious health hazard which is common throughout South Asia and parts of the Middle East. It has been strongly implicated to various benign and malignant lesions of oral cavity and oropharynx. These tobacco-filled leaves deliver more than three times the amount of nicotine, carbon monoxide, and tar as cigarettes which exert injurious effects on cells reflected in terms of accelerated proliferative activity in normal oral mucosal cells. Aim: This study aimed to compare the exfoliated cells from the oral mucosa of bidi smokers and nonsmokers, with emphasis on proliferative activity. Materials and Methods: Exfoliative smears were obtained from the oral mucosa of forty participants (twenty nonsmokers and twenty smokers) with age group ranging from 30-80 years, in and around Barwala (Haryana). The cytologic smears were evaluated using Papanicolaou (PAP) stain and AgNOR in order to evaluate the presence of cytological alterations suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. Only PAP Class I and Class II smears were observed. Results: Comparison of the mean number of AgNORs showed a significant difference between nonsmokers and smokers. Inflammatory alterations were found in 70% of smokers and 55% of nonsmokers. A significant difference in proliferative activity was observed between smokers and nonsmokers classified as PAP Class II. Conclusion: A significant difference of AgNORs/nucleus was observed between bidi smokers and nonsmokers.
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Affiliation(s)
- Tarun Vyas
- Department of Oral Medicine and Radiology, RR Dental College, Udaipur, Rajasthan, India
| | - Parul Verma
- Department of Conservative Dentistry and Endodontics, IDS Sehora, Jammu and Kashmir, India
| | - Mohammed Abidullah
- Department of Oral Pathology and Microbiology, SB Patil Dental College and Hospital, Bider, Karnataka, India
| | - Sandhya Singh Kushwaha
- Department of Oral Pathology and Microbiology, Rishiraj College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India
| | - Pradyumna Kumar Sahoo
- Department of Prosthodontics, Institute of Dental Sciences, Bhubaneswar, Odisha, India
| | - Smita R Priyadarshini
- Department of Oral Medicine and Radiology, Institute of Dental Sciences, Bhubaneswar, Odisha, India
| | - Santosh Kumar Subudhi
- Department of Oral and Maxillofacial Surgery, Institute of Dental Sciences, Bhubaneswar, Odisha, India
| | - Vivek Rana
- Private Practitioner, Lajpat Nagar, New Delhi, India
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Abstract
The carcinogenesis in the oral cavity occurs as a multistep process and is often preceded by potentially malignant lesions. The main risk factors for the development of oral cancer are smoking and alcohol intake. The current challenge is to identify patients at greatest risk for the development of oral cancer using noninvasive and effective methods. The aim of this study is to evaluate the microsatellite mutations in the 9p21 locus, the cell proliferative activity, the pattern of epithelial desquamation, and the nucleus/cytoplasm ratio of exfoliated epithelial cells. Cytopathological samples were collected from 131 individuals divided into four groups: control (n = 26), alcohol-smoking (n = 32), leukoplakia (n = 38), and the oral squamous cell carcinoma group (OSCC, n = 35). From the cytological scraping, a slide was silver impregnated for Ag-stained nucleolar organizer region analysis and another slide was stained using the Papanicolaou technique. The remaining cells were used for DNA extraction, followed by PCR amplification and capillary electrophoresis. The cell proliferation velocity rate was higher in the leukoplakia and OSCC groups compared with the control group (P < 0.05). The leukoplakia group showed increased anucleated scales, whereas the nucleated superficial predominated in the control group and the parabasal cells in the OSCC group (P < 0.05). An increased nucleus/cytoplasm ratio was detected only in the OSCC group (P < 0.05). The 9p21 locus mutation frequency was higher in the alcohol-smoking and leukoplakia groups. 9p21 analysis and Ag-stained nucleolar organizer region methods are promising for the screening and monitoring of individuals at higher risk for the development of oral cancer.
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Souto GR, Matias MDP, Nunes LFM, Ferreira RC, Mesquita RA. Mature dendritic cell density is affected by smoking habit, lesion size, and epithelial dysplasia in oral leukoplakia samples. Arch Oral Biol 2018; 95:51-57. [PMID: 30056280 DOI: 10.1016/j.archoralbio.2018.07.008] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/06/2018] [Revised: 05/24/2018] [Accepted: 07/12/2018] [Indexed: 02/08/2023]
Abstract
OBJECTIVE To compare the densities of CD1a + immature and CD83+ mature dendritic cells, and inflammatory infiltrate cells between smokers and non-smokers with oral leukoplakia. Parameters associated with malignant transformation were also evaluated. DESIGN 21 smokers and 23 non-smokers diagnosed with oral leukoplakia were obtained. Densities of inflammatory infiltrate cells were calculated in H&E sections. Immunohistochemistry using anti-CD1a and anti-CD83 was performed and densities were calculated. Comparisons and statistical analyses were performed among the groups and parameters as gender, lesion size, site, and presence of cell dysplasia were analyzed. RESULTS A lower density of CD83+ cells was observed in smokers compared to non-smokers (P < 0.05). For samples of smokers, a lower density of CD1a + cells, CD83+ cells, and inflammatory infiltrate cells was observed in samples with <10 mm compared to samples ≥10 mm of diameter (P < 0.05), and a lower density of CD83+ cells was also observed between samples without dysplasia compared to samples with dysplasia (P < 0.05). CONCLUSION In oral leukoplakia samples, dendritic cell density decreases in the presence of smoking habit, and increases in larger lesions and with epithelial dysplasia. Smoking habit is an external factor that contribute to alteration of the anti-tumoral immune defense system in lesions of oral leucoplakia, reinforcing that smoking elimination is important to control the development of this disease.
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Affiliation(s)
- Giovanna Ribeiro Souto
- Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Hospital Público Regional de Betim, Betim, Minas Gerais, Brasil.
| | - Michelle Danielle Porto Matias
- Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
| | - Laiz Fernandes Mendes Nunes
- Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
| | - Raquel Conceição Ferreira
- Department of Social and Preventive Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
| | - Ricardo Alves Mesquita
- Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
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da Silva AD, Daroit NB, Cardoso FB, Laureano NK, Maraschin BJ, Bündrich L, Danilevicz CK, Magnusson AS, Visioli F, Rados PV. Epithelial oral mucosal cells: Do they behave differently when exposed to oral carcinogens? Cytopathology 2017; 29:49-57. [PMID: 28960602 DOI: 10.1111/cyt.12468] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 07/19/2017] [Indexed: 11/30/2022]
Abstract
OBJECTIVE To assess the level of maturation and proliferation of epithelial cells and the correlation with immunocytochemical expression of adhesion (E-cadherin) and cell differentiation (involucrin) markers. METHODS Cytopathological samples were obtained from four groups of patients: control (CG, n=30); alcohol/tobacco (ATG, n=31), leucoplakia (LG, n=31), and squamous cell carcinoma (SCCG, n=22). Cytopathological smears were collected from all groups for AgNOR, Papanicolaou and immunocytochemical staining. RESULTS There was an increase in anucleated cells in ATG compared to CG and in LG compared to lesion-free groups (P<.05). In addition, there was a higher rate of intermediate cells in lesion-free groups than in LG (P=.001). When these findings were correlated with positive E-cadherin expression, there was a smaller number of anucleated and intermediate cells (P<.05). The proliferation rate was higher in the SCCG than in the CG (P<.05) and in the ATG compared to LG (P<.05). Moreover, cell proliferation increased in the presence of positive E-cadherin expression in the ATG and LG. No statistically significant results were obtained for involucrin analysis. CONCLUSION Cytopathology combined with quantitative techniques such as Papanicolaou, AgNOR, and immunocytochemical expression of E-cadherin detects changes associated with oral carcinogenesis. The innovative approach used in this study allows assessing the expression of cell adhesion (E-cadherin) and differentiation (involucrin) markers by means of oral mucosal cytopathology. The E-cadherin imunocytochemical expression indicated changes associated with the oral carcinogenesis process. An increase in cell proliferation rate in oral squamous cell carcinoma group was associated with the lower immunoexpression of E-cadherin. Cytopathology combined with quantitative techniques and immunocytochemical expression of E-cadherin may detect early alterations associated with oral carcinogenesis.
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Affiliation(s)
- A D da Silva
- Department of Oral Pathology, Universidade Luterana do Brasil, Canoas, Brazil
| | - N B Daroit
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - F B Cardoso
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - N K Laureano
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - B J Maraschin
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - L Bündrich
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - C K Danilevicz
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - A S Magnusson
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - F Visioli
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - P V Rados
- Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
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Udayashankar U, Guduru VS, Ananthaneni A, Ramisetty SD, Kuberappa PH, Namala S. Evaluation of cytomorphometric changes in tobacco users and diagnosed oral squamous cell carcinoma individuals. J Cytol 2016; 33:125-129. [PMID: 27756983 PMCID: PMC4995868 DOI: 10.4103/0970-9371.188047] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022] Open
Abstract
Aims: To determine the cellular and nuclear area of keratinocytes in smears obtained from the oral mucosa of tobacco users, those with oral squamous cell carcinoma (OSCC), and from normal healthy persons and resolve if any significant difference exists in these three groups. Materials and Methods: The study group comprised 100 subjects 20 controls, (40 OSCC patients-20 from lesional sites and 20 from nonlesional sites, 20 tobacco smokers and 20 tobacco chewers) in the age group of 25-75 years. Oral mucosal smears obtained by using a cytobrush were stained with Papanicolaou (PAP) stain and using 20X objective in trinocular Olympus model BX53 with Jenoptik scientific grade-dedicated microphotographic camera images were taken. With ProgRes version 8.0 image analysis software, 20 cells with defined borders were evaluated from each slide. Finally, one-way analysis of variance (ANOVA) was used to compare the above parameters in the studied groups. Statistical Analysis Used: Minitab and Excel software were used to analyze the data. One-way ANOVA was used to compare the above parameters in the studied groups. Results: The mean value of the cell area for groups I, II, III, IV, and V were 2838 ± 275.2, 2762.1 ± 511.4, 2861.9 ± 512.9, 2643.8 ± 333.3, and 3064.3 ± 362.7, respectively, the nuclear area (NA) was 83.88 ± 9.86, 106.19 ± 13.45, 95.11 ± 14.24, 85.55 ± 21.11, and 80.83 ± 13.45, respectively, and nuclear-to-cellular (N:C) ratio was 0.0297, 0.03924, 0.0337, 0.03257, and 0.02678, respectively. Conclusions: Thus, our study elucidates that cytomorphology gauges the effect of tobacco on the oral mucosa and possibly establishes a link between premalignant and malignant transformations even before a lesion is visibly noted.
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Affiliation(s)
- Urmila Udayashankar
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
| | - Vijay Srinivas Guduru
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
| | - Anuradha Ananthaneni
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
| | - Sabitha Devi Ramisetty
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
| | - Puneeth Horatti Kuberappa
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
| | - Srilekha Namala
- Department of Oral and Maxillofacial Pathology, St. Joseph Dental College, Eluru, Andhra Pradesh, India
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Webber LP, Pellicioli ACA, Magnusson AS, Danilevicz CK, Bueno CC, Sant’Ana Filho M, Rados PV, Carrard VC. Nuclear changes in oral mucosa of alcoholics and crack cocaine users. Hum Exp Toxicol 2015; 35:184-93. [DOI: 10.1177/0960327115579430] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/31/2022]
Abstract
The effects of drugs of abuse on oral mucosa are only partly understood. The aims of the present study were to: (1) evaluate the frequency of nuclear changes in normal-appearing oral mucosa of alcoholics and crack cocaine users and (2) assess their association with cell proliferation rate. Oral smears were obtained from the border of the tongue and floor of the mouth of 26 crack cocaine users (24 males and 2 females), 29 alcoholics (17 males and 12 females), and 35 controls (17 males and 18 females). Histological slides were submitted to Feulgen staining to assess the frequency of micronuclei (MN), binucleated cells (BN), broken eggs (BE), and karyorrhexis (KR). A significant increase in the frequency of MN was observed in cells exfoliated from the tongue of crack cocaine users ( p = 0.01), and alcoholics showed a higher frequency of KR in cells obtained from the floor of the mouth ( p = 0.01). Our findings suggest that the use of crack cocaine induces clastogenic effects, whereas alcoholism is associated with higher degrees of keratinization in the floor of the mouth.
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Affiliation(s)
- LP Webber
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - ACA Pellicioli
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - AS Magnusson
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - CK Danilevicz
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - CC Bueno
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - M Sant’Ana Filho
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - PV Rados
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
| | - VC Carrard
- Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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9
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Gonzalez Segura I, Secchi D, Carrica A, Barello R, Arbelo D, Burgos A, Brunotto M, Zarate AM. Exfoliative cytology as a tool for monitoring pre-malignant and malignant lesions based on combined stains and morphometry techniques. J Oral Pathol Med 2014; 44:178-84. [PMID: 25065639 DOI: 10.1111/jop.12219] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 05/15/2014] [Indexed: 01/09/2023]
Abstract
BACKGROUND Prevention and early diagnosis have the greatest potential for public health and are the most effective method in the long-term to control oral cancer. The aim was to apply PAP staining together with AgNOR staining and morphometric analysis in oral exfoliative cytology, to determine the sensitivity and specificity of these methods in the detection of malignant changes for the purposes of both initial population monitoring and follow-up. METHODS AgNOR, Papanicolau, and morphometric tests were conducted in samples of patients with oral cancer, oral potentially malignant disorders and controls (opposite side of lesions). Specificity and sensitivity values for each stain method and the curve under ROC area were estimated. RESULTS The diagnostic variables which allowed greatest accuracy in identifying malignancy relative to the healthy control were cluster (76.92%), satellite (75.64%), and total (90%). The diagnosis was seen to be associated with PAP and total AgNOR, total AgNOR and PAP, total AgNOR and satellites and clusters, and total AgNOR nuclear area/cytoplasmic area ratio. CONCLUSIONS The total number of AgNOR is a reliable marker for detecting neoplastic cells; this method increases sensitivity and specificity by decreasing the likelihood of false negatives or positives, as the accuracy obtained was 90%. It is also a low-cost, non-invasive, simple methodology that can be recommended to help the early detection of oral cancer and monitoring of patients with a first diagnosis of cancer.
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Detection of epidermal growth factor receptor intensity in asymptomatic fully impacted lower third molar follicles of smoking and nonsmoking patients. J Craniofac Surg 2013; 24:435-8. [PMID: 23524710 DOI: 10.1097/scs.0b013e31828014b2] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022] Open
Abstract
OBJECTIVE The objective of this study was to determine whether smoking causes pathological changes, comparing intensity of epidermal growth factor receptor (EGFR) expression in smokers' and nonsmokers' pericoronal follicles located around asymptomatic impacted lower third molars. STUDY DESIGN Eighty-two dental follicles were collected from asymptomatic mandibular third molars of 41 smoker and 41 nonsmoker patients. Specimens were examined immunohistochemically using antibody against EGFR. RESULTS The expression of EGFR in smokers' pericoronal follicles was higher as compared with nonsmokers (P = 0.036). Also, high EGFR expression was detected in female smokers than in female nonsmokers (P = 0.01). There was a statistically significant correlation between pack-years and EGFR expression intensity in male patients. CONCLUSIONS The risk of pathological differentiation in pericoronal tissues of smoking patients is higher than in the nonsmoking patients. This factor may be taken into account when deciding whether to remove an asymptomatic impacted lower third molar.
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11
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Souto GR, Caliari MV, Lins CEC, De Aguiar MCF, De Abreu MHNG, Mesquita RA. Tobacco use increase the number of aneuploid nuclei in the clinically healthy oral epithelium. J Oral Pathol Med 2010; 39:605-10. [PMID: 20618610 DOI: 10.1111/j.1600-0714.2010.00907.x] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
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Ahmed HG, Babiker AEA. Assessment of cytological atypia, AgNOR and nuclear area in epithelial cells of normal oral mucosa exposed to toombak and smoking. Rare Tumors 2009; 1:e18. [PMID: 21139889 PMCID: PMC2994448 DOI: 10.4081/rt.2009.e18] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/29/2009] [Accepted: 06/30/2009] [Indexed: 11/23/2022] Open
Abstract
The purpose of this study was to assess cellular proliferative activity of clinically healthy oral mucosal epithelial cells of toombak dippers and smokers by means of AgNOR counts and nuclear areas via nuclear morphometry. Smears were collected from normal-appearing mouth floor mucosa and tongue of 75 toombak dippers, 75 smokers and 50 non-tobacco users between the ages of 20 and 70 with a mean age of 36 years. AgNORs were counted in the first 50 well-fixed, nucleated squamous cells and nuclear areas were calculated via microscopic stage micrometer. Cytological atypia was ascertained in 6 tobacco users and could not be ascertained in non-tobacco users. Statistically mean AgNOR numbers per nucleus in the non-tobacco users (2.45±0.30) was lower than the toombak dippers (3.081±0.39, p<0.004), and the smokers (2.715±0.39, p<0.02), and mean nuclear areas of epithelial cells of toombak dippers (6.081±0.39, p<0.009) and smokers (5.68±10.08, p<0.01) was also significantly higher than non-smokers (5.39±9.4). The mean number of nuclei having more than 3 AgNORs was 28%, 19% and 7% in toombak dippers, smokers and non-tobacco users, respectively. These findings support the view that toombak dipping and smoking are severe risk factors for oral mucosal proliferative lesions and exfoliative cytology is valid for screening of oral mucosal lesions.
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Affiliation(s)
- Hussain Gadelkarim Ahmed
- Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan
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13
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Fontes PC, Corrêa GHM, Issa JS, Brandão AAH, Almeida JD. Comparison of exfoliative pap stain and AgNOR counts of the tongue in smokers and nonsmokers. Head Neck Pathol 2008; 2:157-62. [PMID: 20614310 PMCID: PMC2807565 DOI: 10.1007/s12105-008-0059-0] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/27/2008] [Accepted: 04/30/2008] [Indexed: 12/01/2022]
Abstract
OBJECTIVE To compare exfoliative cytology from the oral mucosa of smokers and nonsmokers, with emphasis on proliferative activity. METHODS Exfoliative cytology specimens were obtained from clinical normal mucosa from the lateral border of the tongue in 30 nonsmokers and 30 smokers ranging in age from 40 to 70 years of age, who were seen at the Heart Institute's Patient Center and the Smoking Cessation Program of the University Hospital, University of São Paulo Medical School (InCor-HCFMUSP). The cytologic specimens were evaluated by Papanicolaou staining and AgNOR quantification in order to evaluate the presence of cytological alterations suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. RESULTS Only Papanicolaou Class I and Class II smears were observed. Inflammatory alterations were found in 90% of smokers and in 87% of nonsmokers. The number of AgNORs/nucleus differed significantly between smokers and nonsmokers (3.372 +/- 0.375 versus 2.732 +/- 0.236). CONCLUSIONS Within the limitations of this research, the results indicate higher proliferative activity in smoking patients compared to nonsmoking patients, even in the absence of clinical lesions.
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Affiliation(s)
- Patrícia Campos Fontes
- Postgraduation Program in Oral Biopathology, São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Sao Paulo, Brazil
| | | | - Jaqueline Scholz Issa
- Smoking Cessation Program, Heart Institute (InCor), Medical School, University of São Paulo, Sao Paulo, SP Brazil
| | - Adriana Aigotti Haberbeck Brandão
- Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Av. Francisco José Longo, 777 São Dimas, Sao Paulo, 12245-000 Brazil
| | - Janete Dias Almeida
- Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Av. Francisco José Longo, 777 São Dimas, Sao Paulo, 12245-000 Brazil
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14
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Burzlaff JB, Bohrer PL, Paiva RL, Visioli F, Sant'Ana Filho M, da Silva VD, Rados PV. Exposure to alcohol or tobacco affects the pattern of maturation in oral mucosal cells: a cytohistological study. Cytopathology 2007; 18:367-75. [PMID: 17680816 DOI: 10.1111/j.1365-2303.2007.00473.x] [Citation(s) in RCA: 16] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Abstract
OBJECTIVE To assess the maturation pattern of oral mucosal cells of patients exposed to tobacco and alcohol. METHODS (i) Group without lesions. Smears obtained from the lower lip, border of the tongue and floor of the mouth of 31 control individuals (group I), 49 tobacco users (group II) and 27 tobacco/alcohol users (group III) were stained using the Papanicolaou method. The first 100 cells counted on each smear determined the maturation pattern and the keratinization index (KI). Analysis of variance (ANOVA) and the Tukey multiple comparison test were used for statistical analysis, at a 5% significance level. (ii) Group with lesions. Cytopathological and histopathological studies were conducted for 15 patients: eight with leucoplakia without epithelial dysplasia, two with epithelial dysplasia and five with squamous cell carcinoma. RESULTS (i) Group without lesions. Statistical analysis revealed a smaller number of superficial cells with nuclei in all sites of the group of tobacco/alcohol users (group III) when compared to the control group (group I), and this difference was statistically significant (P<0.005). (ii) Group with lesions. The severity of histopathological findings increased with the increase in the number of cells of the deeper epithelial layers, with a statistically significant difference in the number of intermediate (P=0.013) and parabasal cells (P=0.049), which increased with the severity of the epithelial maturation disorder: leucoplakias with dysplasia had a greater number of intermediate and parabasal cells than leucoplakias without dysplasia; and the number in squamous cell carcinomas was greater than in leucoplakias with dysplasia. CONCLUSION The maturation pattern of cells in the three anatomic sites showed changes that may be associated with the synergistic effect of tobacco and alcohol. Also, the severity of histopathological findings was associated with the increase in the number of cells in the deeper epithelial layers.
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Affiliation(s)
- J B Burzlaff
- Graduate Program in Dentistry, Department of Oral Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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Chang HW, Ling GS, Wei WI, Yuen APW. Smoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma. Cancer 2004; 101:125-32. [PMID: 15221997 DOI: 10.1002/cncr.20323] [Citation(s) in RCA: 54] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022]
Abstract
BACKGROUND The consumption of tobacco and alcohol has been implicated in the development of head and neck squamous cell carcinoma (HNSCC). Promoter methylation of tumor suppressor genes is common in HNSCC. In this study, the authors evaluated the effects of tobacco and alcohol on p15 gene methylation of cells in cells from the mouth and throat of physically healthy individuals and patients with HNSCC. METHODS The study participants were divided into 3 groups, including a group of 37 healthy nonsmokers and nondrinkers, a group of 22 healthy smokers and/or drinkers and a group of 31 patients with HNSCC. RESULTS Methylation of p15 was detected in cells obtained from mouth and throat (M&T) rinsing fluid samples from 3 of 37 healthy individuals (8%) who did not drink or smoke, from 15 of 22 healthy smokers and/or drinkers (68%), and from 15 of 31 patients (48%) with HNSCC. Among 31 patients with HNSCC, 20 patients (65%) had methylated p15 gene in their tumor biopsies. With the use of beta-actin as a reference, the ratio of methylated p15 to beta-actin was calculated as an index of the percentage of cells with p15 methylation. The percentage of exfoliated cells from M&T rinsing fluid samples that had p15 methylation ranged from 0% to 11% for patients with HNSCC and from 0% to 21% for healthy smokers/drinkers, respectively. The methylation index of tumor cells with p15 methylation ranged from 0% to 65%. CONCLUSIONS The results suggest that p15 gene methylation can be induced by chronic smoking and drinking and may play a role in the very early stages of carcinogenesis in HNSCC.
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Affiliation(s)
- Hsiao Wen Chang
- Department of Surgery, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong SAR, China
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Schwartz JL, Muscat JE, Baker V, Larios E, Stephenson GD, Guo W, Xie T, Gu X, Chung FL. Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis shows differences between smokers and non-smokers. Oral Oncol 2003; 39:842-54. [PMID: 13679208 DOI: 10.1016/s1368-8375(03)00107-6] [Citation(s) in RCA: 33] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/27/2022]
Abstract
Oral cytology and morphometric staining is used to identify malignant keratinocytes in oral premalignant or malignant lesions. To detect and to begin to assess changes in oral keratinocytes exposed to tobacco-derived carcinogens, which are at risk for malignant transformation, a novel method is required. The approach uses oral cytology harvested oral keratinocytes analyzed using flow cytometry (FC) for changes in DNA content, damage, cell cycle and apoptosis. Six smoker and six non-smoker oral keratinocytes were evaluated using flow cytometry in the form of laser scanning cytometry (LSC) and laser microdissection (LMD). Among smokers compared to non-smokers, the method detected and assessed DNA damage from tobacco smoke exposure quantifying an enhanced formation of DNA adducts, such as, 8-hydroxy-2'-deoxyguanine (8-OHdG) which creates oxidation lesions and benzo[a]pyrene(B[a]P), which produces a B[a]P)-N2-dG bulky adduct. Increased DNA content, aneuploidy, percentage of cells in synthesis (S) and G(2)+Mitosis (M), and apoptosis were recorded. Tissue and cell controls were used to verify these relationships. Data suggested healthy smokers were at increased risk for malignant transformation of oral keratinocytes because of the changes stated above. Using identical methods, keratinocytes exposed to the tobacco derived carcinogen, B[a]P parallel results obtained from smoke exposure indicating a direct link. Flow cytometric evaluation of oral cytology harvested keratinocytes can be used to measure exposure to tobacco carcinogens, and possibly establish a link to premalignant and malignant transformation before a lesion is noted.
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Affiliation(s)
- Joel L Schwartz
- Institute for Cancer Prevention, One Dana Road, Valhalla, NY 10595, USA.
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