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Lehmann K, Kadler G, Kalyanov A, Schweizer TA, Walt H, Essig H. Zirconium Dental Implants as Potential Optical Waveguides in Photodynamic Inactivation of Bacterial Biofilms-A Pilot Study. Microorganisms 2025; 13:850. [PMID: 40284686 PMCID: PMC12029833 DOI: 10.3390/microorganisms13040850] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/26/2025] [Revised: 03/27/2025] [Accepted: 04/03/2025] [Indexed: 04/29/2025] Open
Abstract
In patients with predisposing risk factors, bacterial colonization of dental implants can lead to periimplantitis (PI). Established individual treatment protocols can be effective, but antimicrobial resistance (AMR) and biofilm formation may impede successful treatment, therefore requiring surgical intervention. Photodynamic Inactivation (PDI) combined with optical waveguides could eradicate such pathogens without the risk of new AMR emergence and reduce the need for surgery. In this pilot study, we investigated the waveguiding function of light-transmitting zirconium dioxide (ZrO2) dental implants of different diameters by quantifying their transmission spectrum, fraction of transmitted red-light intensity, and potential polarizing properties. In addition, PDI experiments involving in vitro grown Staphylococcus epidermidis biofilms on ZrO2 and titanium alloy (TAV) discs were performed. Colonized discs were treated with Methylene Blue (MB) photosensitizer before red-light illumination (670 nm) at various intensities. A reduction in bacterial colony-forming units (CFUs) of up to 85% was observed on ZrO2 discs. Meanwhile, the biofilms grown on TAV discs showed no significant reduction in CFUs. These findings make ZrO2 a potential candidate in augmentative PDI treatment of PI. The successful use of PDI combined with waveguiding ZrO2 dental implants can support the reduction in antibiotic prescriptions, thus advancing the WHO's One Health approach of antibiotic stewardship.
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Affiliation(s)
- Kolja Lehmann
- Department of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland; (K.L.); (G.K.)
| | - Gabor Kadler
- Department of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland; (K.L.); (G.K.)
| | - Alexander Kalyanov
- Biomedical Optics Research Laboratory, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland
| | - Tiziano A. Schweizer
- Department of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland; (K.L.); (G.K.)
- Department of Dermatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland
| | - Heinrich Walt
- Department of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland; (K.L.); (G.K.)
| | - Harald Essig
- Department of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland; (K.L.); (G.K.)
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Yamaguchi Y, Yoshii D, Katsuragi H, Shinkai K. Effect of Laser Irradiation Modes and Photosensitizer Types on Antimicrobial Photodynamic Therapy (aPDT) for Streptococcus sobrinus in the Crown Dentin of Bovine Teeth: An Experimental In Vitro Study. Dent J (Basel) 2024; 12:59. [PMID: 38534283 DOI: 10.3390/dj12030059] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2023] [Revised: 02/27/2024] [Accepted: 02/28/2024] [Indexed: 03/28/2024] Open
Abstract
This study aimed to assess the impact of different laser irradiation modes and photosensitizer types on the bactericidal efficacy of antimicrobial photodynamic therapy (aPDT). Dentin plates were prepared by sectioning the crown dentin of bovine teeth infected with Streptococcus sobrinus (n = 11). Nine aPDTs involving the combination of three 1% solutions of photosensitizers (brilliant blue, BB; acid red, AR; and methylene blue, MB) and three irradiation modes of semiconductor lasers (50 mW for 120 s, 100 mW for 60 s, and 200 mW for 30 s) were performed for each infected dentin plate, and the control consisted of the specimens not applied with aPDT. The bactericidal effects in 10 groups were evaluated using both assays of the colony count (colony-forming-unit: CFU) and adenosine triphosphate (ATP) (relative-light-unit: RLU). The data obtained were analyzed using the Kruskal-Wallis test (α = 0.05). The most aPDT groups exhibited significantly lower RLU and CFU values compared with the control (p < 0.05). The effect of irradiation modes on RLU and CFU values was significant in the aPDT group using BB (p < 0.05) but not in the aPDT group using AR or MB. The aPDT performed with AR or MB exerted a remarkable bactericidal effect.
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Affiliation(s)
- Yohei Yamaguchi
- Advance Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan
| | - Daiki Yoshii
- Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan
| | - Hiroaki Katsuragi
- Research Center for Odontology, The Nippon Dental University School of Life Dentistry, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan
| | - Koichi Shinkai
- Advance Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan
- Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan
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Takahashi T, Sato F, Shinkai K. The Effects of Antimicrobial Photodynamic Therapy Used to Sterilize Carious Dentin on Rat Dental Pulp Tissue. Dent J (Basel) 2023; 11:283. [PMID: 38132421 PMCID: PMC10743003 DOI: 10.3390/dj11120283] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/19/2023] [Revised: 11/28/2023] [Accepted: 12/04/2023] [Indexed: 12/23/2023] Open
Abstract
Antimicrobial photodynamic therapy (aPDT) used to sterilize carious dentin may irritate pulp tissues because of tissue-penetrating laser and singlet oxygen generation. This study aimed to assess the effects of aPDT on rat pulp tissues. A cavity formed in a rat maxillary first molar was treated with aPDT. The combined photosensitizer and laser irradiation conditions in the aPDT groups were as follows: methylene blue and 100 mW for 60 s, brilliant blue (BB) and 100 mW for 60 s, BB and 50 mW for 120 s, and BB and 200 mW for 30 s. Each cavity was treated with an all-in-one adhesive and filled with flowable resin. aPDT was not applied for the control. In each group, the rats were sacrificed on postoperative days 1 and 14, and thin sections of the treated teeth were prepared. Pulp tissue disorganization (PTD), inflammatory cell infiltration (ICI), and tertiary dentin formation (TDF) were evaluated. At 1-day evaluation, there were significant differences between the aPDT group and controls with respect to PTD and ICI (p < 0.01); 14 days later, almost all specimens showed tertiary dentin formation. The application of aPDT caused reversible damage to the rat pulp, while in the long term, healing occurred with the formation of tertiary dentin.
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Affiliation(s)
- Tenyu Takahashi
- Advanced Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;
| | - Fumiaki Sato
- Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;
| | - Koichi Shinkai
- Advanced Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;
- Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;
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Utkina EI, Gorbatova MA, Grjibovski AM, Gorbatova LN, Simakova AA. [Potentialities of photoactivated disinfection in dentistry]. STOMATOLOGIIA 2023; 102:84-90. [PMID: 37144773 DOI: 10.17116/stomat202310202184] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/06/2023]
Abstract
Infection control is an essential part of dental practice. Oral antiseptics should be highly effective against the most common oral pathogens without causing microbial resistance, biocompatible with the human tissues with no interaction with fillings materials. Photoactivated disinfection (PAD) is based on activation of photosensitizers - specific substances releasing active oxygen forms after absorption of the light. The active oxygen forms destroy bacterial cell structures without affecting human cells. The overwhelming majority of Russian and international researchers have reported high effectiveness of PAD in periodontics, implantology and endodontics, whereas the use of PAD in caries treatment and prevention is not so fully understood yet. Earlier studies have demonstrated high sensitivity of cariogenic bacteria to PAD, suggesting it as an additional minimally invasive caries therapy improving treatment effectiveness. PAD spares dental tissues without reduction in the effectiveness of disinfection. It is particularly important in treatment of deep carious lesions and disinfection of thin dentine layer near the pulp. Effectiveness of PAD in caries treatment has been demonstrated for both permanent and deciduous dentition. PAD doesn't affect bond strength to fillings, improves plasticity of dental pulp and dental hard tissues mineralization in children. Effective control of a wide range of bacteria without causing resistance makes PAD a prospective method of treatment and prevention of caries.
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Affiliation(s)
- E I Utkina
- Northern State Medical University, Arkhangelsk, Russia
| | - M A Gorbatova
- Northern State Medical University, Arkhangelsk, Russia
| | - A M Grjibovski
- Northern State Medical University, Arkhangelsk, Russia
- West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan
- Ammosov North-Eastern Federal University, Yakutsk, Russia
| | - L N Gorbatova
- Northern State Medical University, Arkhangelsk, Russia
| | - A A Simakova
- Northern State Medical University, Arkhangelsk, Russia
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Clinical and radiographic evaluation of indirect pulp treatment of young permanent molars using photo-activated oral disinfection versus calcium hydroxide: a randomized controlled pilot trial. BDJ Open 2020; 6:4. [PMID: 32194986 PMCID: PMC7078204 DOI: 10.1038/s41405-020-0030-z] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/23/2019] [Revised: 12/28/2019] [Accepted: 01/03/2020] [Indexed: 11/25/2022] Open
Abstract
Background Calcium hydroxide is the most commonly used material in indirect pulp treatment (IPT). However, its drawbacks required its replacement by other materials. Aim This study aims to estimate clinically and radiographically the success of indirect pulp treatment of young permanent molars with either photo-activated oral disinfection (PAD) or calcium hydroxide. Design This Randomized Controlled Pilot Trial included 32 vital first permanent molars with deep caries that were treated by indirect pulp treatment with either PAD (group 1) or calcium hydroxide (group 2). Clinical and radiographic success in addition to newly-formed dentin thickness were evaluated regularly at 2, 6, 9, and 12 months. Results The success for both groups was 100% clinically and radiographically at all follow-up periods. Regarding the mean thickness of newly-formed dentin for both groups at different follow-up periods, there was no statistically significant difference between both groups at 2, 6, 9, and 12 months, with P values = 0.825, 0.146, 0.280, and 0.400, respectively. Conclusions The clinical and radiographic success for indirect pulp treatment of young permanent molars with both PAD and calcium hydroxide were comparable.
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Prosa M, Bolognesi M, Fornasari L, Grasso G, Lopez-Sanchez L, Marabelli F, Toffanin S. Nanostructured Organic/Hybrid Materials and Components in Miniaturized Optical and Chemical Sensors. NANOMATERIALS (BASEL, SWITZERLAND) 2020; 10:E480. [PMID: 32155993 PMCID: PMC7153587 DOI: 10.3390/nano10030480] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/08/2020] [Revised: 02/28/2020] [Accepted: 03/04/2020] [Indexed: 01/16/2023]
Abstract
In the last decade, biochemical sensors have brought a disruptive breakthrough in analytical chemistry and microbiology due the advent of technologically advanced systems conceived to respond to specific applications. From the design of a multitude of different detection modalities, several classes of sensor have been developed over the years. However, to date they have been hardly used in point-of-care or in-field applications, where cost and portability are of primary concern. In the present review we report on the use of nanostructured organic and hybrid compounds in optoelectronic, electrochemical and plasmonic components as constituting elements of miniaturized and easy-to-integrate biochemical sensors. We show how the targeted design, synthesis and nanostructuring of organic and hybrid materials have enabled enormous progress not only in terms of modulation and optimization of the sensor capabilities and performance when used as active materials, but also in the architecture of the detection schemes when used as structural/packing components. With a particular focus on optoelectronic, chemical and plasmonic components for sensing, we highlight that the new concept of having highly-integrated architectures through a system-engineering approach may enable the full expression of the potential of the sensing systems in real-setting applications in terms of fast-response, high sensitivity and multiplexity at low-cost and ease of portability.
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Affiliation(s)
- Mario Prosa
- Institute of Nanostructured Materials (ISMN), National Research Council (CNR), via P. Gobetti 101, 40129 Bologna, Italy; (M.P.); (M.B.)
| | - Margherita Bolognesi
- Institute of Nanostructured Materials (ISMN), National Research Council (CNR), via P. Gobetti 101, 40129 Bologna, Italy; (M.P.); (M.B.)
| | - Lucia Fornasari
- Plasmore s.r.l., viale Vittorio Emanuele II 4, 27100 Pavia, Italy; (L.F.); (L.L.-S.)
| | - Gerardo Grasso
- Institute of Nanostructured Materials (ISMN), National Research Council (CNR) c/o Department of Chemistry, ‘Sapienza’ University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy;
| | - Laura Lopez-Sanchez
- Plasmore s.r.l., viale Vittorio Emanuele II 4, 27100 Pavia, Italy; (L.F.); (L.L.-S.)
| | - Franco Marabelli
- Physics Department, University of Pavia, via A. Bassi 6, 27100 Pavia, Italy;
| | - Stefano Toffanin
- Institute of Nanostructured Materials (ISMN), National Research Council (CNR), via P. Gobetti 101, 40129 Bologna, Italy; (M.P.); (M.B.)
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Wenzler JS, Böcher S, Frankenberger R, Braun A. Feasibility of transgingival laser irradiation for antimicrobial photodynamic therapy. Photodiagnosis Photodyn Ther 2019; 28:75-79. [PMID: 31470119 DOI: 10.1016/j.pdpdt.2019.08.030] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/03/2019] [Revised: 08/08/2019] [Accepted: 08/26/2019] [Indexed: 11/16/2022]
Abstract
AIM Diode lasers are commonly used for antimicrobial photodynamic therapy (aPDT). This study aimed to assess the feasibility of transgingival laser irradiation during aPDT and evaluate whether the photosensitizer can be activated. MATERIALS AND METHODS Four diode laser settings were assessed for transgingival irradiation: 120 mW, 80 mW, 60 mW, and 40 mW. Fifteen soft-tissue pieces from a pig's lower jaw were prepared. The specimens' thickness was measured and transgingival laser irradiation was performed. A digital power meter measured laser power on the other side of the tissue. The power outcome after staining of the nonbuccal aspect of the tissue with photosensitizer dye was assessed similarly. RESULTS Transgingival laser irradiation (average soft-tissue thickness: 0.84 ± 0.06 mm) resulted in different power transmission depending on the power settings and photosensitizer. The lowest values were observed with the 40 mW setting and photosensitizer (median 3.3 mW, max. 5.0 mW, min. 2.3 mW, interquartile range 1.2), and the highest at 120 mW without photosensitizer (median 41.3 mW, max. 42.7 mW, min. 38.0 mW; interquartile range 1.5). CONCLUSIONS This study indicates that transgingival irradiation may be suitable for aPDT, since power transmission through the gingival tissue was observed in all specimens. However, the decrease in laser power caused by both the soft tissue and the photosensitizer has to be taken into account.
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Affiliation(s)
- Johannes-Simon Wenzler
- Clinic for Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Aachen, Aachen, Germany.
| | - Sarah Böcher
- Clinic for Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Aachen, Aachen, Germany
| | - Roland Frankenberger
- Department of Operative Dentistry and Endodontology, University of Marburg, Marburg, Germany
| | - Andreas Braun
- Clinic for Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Aachen, Aachen, Germany
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Dentinal Hypersensitivity Treatment Using Diode Laser 980 nm: In Vivo Study. Dent J (Basel) 2019; 7:dj7010005. [PMID: 30634421 PMCID: PMC6473910 DOI: 10.3390/dj7010005] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/08/2018] [Revised: 12/11/2018] [Accepted: 01/04/2019] [Indexed: 11/24/2022] Open
Abstract
The discomfort of patients due to dentinal hypersensitivity (DH) is one of the main challenges that dentists face in daily practice. Difficulties in DH treatment gave rise to many protocols which are currently used. The aim of this clinical study is to evaluate the effectiveness of a new protocol on the reduction of dentinal hypersensitivity with diode laser 980 nm and the application of a graphite paste. 184 patients enrolled in the study, the degree of pain was evaluated by visual analog scale (VAS), graphite paste was applied on the exposed dentine before irradiation, the application of diode laser 980 nm with continuous mode, backward motion, tangential incidence of the beam in non-contact mode and a delivery output of 1 W. Fiber’s diameter was 320 μm and total exposure time depended on the time necessary to remove the graphite paste from the teeth. Statistical analyses were performed with Prism 5® software. Pain in post-operative significantly decreased immediately after the treatment. Mean values stayed stable until a 6-month follow-up. The application is considered to be safe with long-term effectiveness.
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Cieplik F, Deng D, Crielaard W, Buchalla W, Hellwig E, Al-Ahmad A, Maisch T. Antimicrobial photodynamic therapy - what we know and what we don't. Crit Rev Microbiol 2018; 44:571-589. [PMID: 29749263 DOI: 10.1080/1040841x.2018.1467876] [Citation(s) in RCA: 511] [Impact Index Per Article: 73.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Abstract
Considering increasing number of pathogens resistant towards commonly used antibiotics as well as antiseptics, there is a pressing need for antimicrobial approaches that are capable of inactivating pathogens efficiently without the risk of inducing resistances. In this regard, an alternative approach is the antimicrobial photodynamic therapy (aPDT). The antimicrobial effect of aPDT is based on the principle that visible light activates a per se non-toxic molecule, the so-called photosensitizer (PS), resulting in generation of reactive oxygen species that kill bacteria unselectively via an oxidative burst. During the last 10-20 years, there has been extensive in vitro research on novel PS as well as light sources, which is now to be translated into clinics. In this review, we aim to provide an overview about the history of aPDT, its fundamental photochemical and photophysical mechanisms as well as photosensitizers and light sources that are currently applied for aPDT in vitro. Furthermore, the potential of resistances towards aPDT is extensively discussed and implications for proper comparison of in vitro studies regarding aPDT as well as for potential application fields in clinical practice are given. Overall, this review shall provide an outlook on future research directions needed for successful translation of promising in vitro results in aPDT towards clinical practice.
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Affiliation(s)
- Fabian Cieplik
- a Department of Conservative Dentistry and Periodontology , University Medical Center Regensburg , Regensburg , Germany.,b Department of Preventive Dentistry , Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands
| | - Dongmei Deng
- b Department of Preventive Dentistry , Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands
| | - Wim Crielaard
- b Department of Preventive Dentistry , Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands
| | - Wolfgang Buchalla
- a Department of Conservative Dentistry and Periodontology , University Medical Center Regensburg , Regensburg , Germany
| | - Elmar Hellwig
- c Department of Operative Dentistry and Periodontology, Faculty of Medicine , Center for Dental Medicine, University of Freiburg , Freiburg , Germany
| | - Ali Al-Ahmad
- c Department of Operative Dentistry and Periodontology, Faculty of Medicine , Center for Dental Medicine, University of Freiburg , Freiburg , Germany
| | - Tim Maisch
- d Department of Dermatology , University Medical Center Regensburg , Regensburg , Germany
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Cieplik F, Buchalla W, Hellwig E, Al-Ahmad A, Hiller KA, Maisch T, Karygianni L. Antimicrobial photodynamic therapy as an adjunct for treatment of deep carious lesions-A systematic review. Photodiagnosis Photodyn Ther 2017; 18:54-62. [PMID: 28099873 DOI: 10.1016/j.pdpdt.2017.01.005] [Citation(s) in RCA: 55] [Impact Index Per Article: 6.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2016] [Revised: 12/29/2016] [Accepted: 01/13/2017] [Indexed: 11/15/2022]
Abstract
For deep carious lesions, a more conservative treatment modality ("selective caries removal") has been proposed, where only the heavily contaminated dentine is removed. In this regard, effective adjuncts for cavity disinfection such as the antimicrobial photodynamic therapy (aPDT) can be valuable clinically prior to definitive restoration. Therefore, the aim of this study was to systematically assess clinical studies on the effectiveness of aPDT as a supplementary tool in the treatment of deep caries lesions. Searches were performed in four databases (PubMed, EMBASE, ISI Web of Science, ClinicalTrials.gov) from 1st January, 2011 until 21st June, 2016 for search terms relevant to the observed parameters, pathological condition, intervention and anatomic entity. The pooled information was evaluated according to PRISMA guidelines. At first, 1651 articles were recovered, of which 1249 full-text articles were evaluated, 270 articles thereof were reviewed for eligibility and finally 6 articles met all inclusion criteria. The aPDT protocols involved Methylene Blue, Toluidine Blue and aluminium-chloride-phthalocyanine as photosensitizers and diode lasers, light-emitting diodes and halogen light-sources. The data from five reports, utilizing both culture-dependent and -independent methods, disclosed significant reduction of cariogenic bacterial load after mechanical caries removal with adjunct aPDT. As these studies exhibit some methodological limitations, e.g. lack of positive controls, this systematic review can support the application of aPDT to a limited extent only in terms of reducing the microbial load in deep carious lesions before restorative treatment.
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Affiliation(s)
- Fabian Cieplik
- Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany.
| | - Wolfgang Buchalla
- Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany
| | - Elmar Hellwig
- Department of Operative Dentistry and Periodontology, Center for Dental Medicine, University of Freiburg, Freiburg, Germany
| | - Ali Al-Ahmad
- Department of Operative Dentistry and Periodontology, Center for Dental Medicine, University of Freiburg, Freiburg, Germany
| | - Karl-Anton Hiller
- Department of Conservative Dentistry and Periodontology, University Medical Center Regensburg, Regensburg, Germany
| | - Tim Maisch
- Department of Dermatology, University Medical Center Regensburg, Regensburg, Germany
| | - Lamprini Karygianni
- Department of Operative Dentistry and Periodontology, Center for Dental Medicine, University of Freiburg, Freiburg, Germany
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Mirzaie M, Yassini E, Ashnagar S, Hadadi A, Chiniforush N. Evaluation of temperature change during antimicrobial photodynamic therapy with two different photosensitizers in dental caries. Photodiagnosis Photodyn Ther 2016; 14:115-8. [PMID: 27012319 DOI: 10.1016/j.pdpdt.2016.03.005] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/17/2016] [Revised: 03/14/2016] [Accepted: 03/16/2016] [Indexed: 11/16/2022]
Abstract
INTRODUCTION Many attempts have been made in elimination of bacteria in infected and demineralized dentin to not only provide efficient bactericidal potential, but to have minimal damage for tooth structure. The aim of this study was to assess the temperature change during aPDT with ICG and TBO compared with conventional Diode laser irradiation. MATERIALS AND METHODS 48 premolar teeth which were selected for this study. A class I cavity was drilled in each teeth, with dimensions of 2mm width, 4mm length and depth of 2.5-3.5mm, providing a dentinal wall of approximately 0.5mm for pulp chamber. Then teeth were randomly allocated in 4 experimental groups (n=12); Group 1: TBO+LED, Group 2: ICG+Diode Laser, Group 3: Diode laser with output power of 0.5W, Group 4: Diode laser with output power of 1W. Thermocouple device was held by experimenter hand and the sensor was in pulp chamber of the teeth. Heat generated during irradiation was reported on LCD screen by NUX Plus software. Repeated measure ANOVA was used in order to compare the temperature before and after laser application. Tukey HSD was used to compare the results between groups. RESULTS Temperature was risen for about 0.54±0.05°C for group 1, 1.67±0.14°C for group 2, 4.21±0.83°C for group 3, and 4. 50±0.32°C for group 4. The difference between group 1 & 2, 1 & 3 and 1 & 4 was significant (p<0.0001). CONCLUSION According to results of this study, Diode (with or without photosensitizer- 0.5 and 1W) can be safely used as alternative approach for disinfection after caries removal in thermal point of view.
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Affiliation(s)
- Mansoreh Mirzaie
- Restorative Dentistry Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
| | - Esmael Yassini
- Restorative Dentistry Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
| | - Sajjad Ashnagar
- Department of Periodontics and Oral Medicine, Michigan School of Dentistry, MI, USA
| | | | - Nasim Chiniforush
- Laser Research Center of Dentistry, Dental Research Institute, Tehran University of Medial Sciences, Tehran, Iran.
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Sharma S, Logani A, Shah N. Comparative efficacy of photo-activated disinfection and calcium hydroxide for disinfection of remaining carious dentin in deep cavities: a clinical study. Restor Dent Endod 2014; 39:195-200. [PMID: 25110643 PMCID: PMC4125583 DOI: 10.5395/rde.2014.39.3.195] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/14/2014] [Accepted: 04/25/2014] [Indexed: 11/11/2022] Open
Abstract
OBJECTIVES To comparatively evaluate the efficacy of photo-activated disinfection (PAD), calcium hydroxide (CH) and their combination on the treatment outcome of indirect pulp treatment (IPT). MATERIALS AND METHODS Institutional ethical clearance and informed consent of the patients were taken. The study was also registered with clinical registry of India. Sixty permanent molars exhibiting deep occlusal carious lesion in patients with the age range of 18 - 22 yr were included. Clinical and radiographic evaluation and set inclusion and exclusion criteria's were followed. Gross caries excavation was accomplished. In group I (n = 20) PAD was applied for sixty seconds. In group II (n = 20), CH was applied to the remaining carious dentin, while in group III (n = 20), PAD application was followed by CH placement. The teeth were permanently restored. They were clinically and radiographically followed-up at 45 day, 6 mon and 12 mon. Relative density of the remaining affected dentin was measured by 'Radiovisiography (RVG) densitometric' analysis. RESULTS Successful outcome with an increase in radiographic grey values were observed in all three groups. However, on inter-group comparison, this change was not significant (p > 0.05). CONCLUSIONS PAD and CH both have equal disinfection efficacy in the treatment of deep carious dentin. PAD alone is as effective for treatment of deep carious lesion as calcium hydroxide and hence can be used as an alternative to CH. They can be used independently in IPT, since combining both does not offer any additional therapeutic benefits.
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Affiliation(s)
- Sidhartha Sharma
- Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, Ansari nagar, New Delhi, India
| | - Ajay Logani
- Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, Ansari nagar, New Delhi, India
| | - Naseem Shah
- Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, Ansari nagar, New Delhi, India
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Umana M, Heysselaer D, Tielemans M, Compere P, Zeinoun T, Nammour S. Dentinal tubules sealing by means of diode lasers (810 and 980 nm): a preliminary in vitro study. Photomed Laser Surg 2013; 31:307-14. [PMID: 23756100 DOI: 10.1089/pho.2012.3443] [Citation(s) in RCA: 40] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
OBJECTIVE The aim of this study was to evaluate the effect on dentinal surfaces of diode lasers (810 and 980 nm) at different parameters. MATERIALS AND METHODS Twenty-four caries-free human impacted wisdom teeth were used. The crowns were sectioned transversely in order to expose the dentin. The smear layer was removed by a 1 min application of ethylenediaminetetraacetic acid (EDTA). Each surface was divided into four quadrants irradiated at a different output power setting for each kind of laser: 0.8, 1, 1.6, and 2 W (energy densities: 2547, 3184, 5092, and 6366 J/cm(2), irradiation speed 1 mm/sec; optical fiber diameter: 200 μm; continuous and noncontact mode). Half of the samples were stained with a graphite paste. All specimens were sent for scanning electron microscopic (SEM) analysis. Pulp temperature increases in additional 20 teeth were measured by a thermocouple. RESULTS Diode laser irradiations at 0.8 and 1 W led to occlusion or narrowing of dentin tubules without provoking fissures or cracks. The application of graphite paste increased the thermal effects in dentin. Measurements of pulp temperature showed that irradiations at 0.8 and 1 W for a period of 10 sec in continuous mode increased pulp temperature (T ≤2°C). CONCLUSIONS Diode lasers (810 and 980 nm) used at 0.8 and 1 W for 10 sec in continuous mode were able to seal the dentin tubules. These parameters can be considered harmless for pulp vitality, and may be effective in the treatment of dentinal hypersensitivity.
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Affiliation(s)
- Monica Umana
- Department of Dental Sciences, Faculty of Medicine, University of Liège , Quai Godfroid Kurth, Liège, Belgium.
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Vescovi P, Merigo E, Fornaini C, Rocca JP, Nammour S. Thermal increase in the oral mucosa and in the jawbone during Nd:YAG laser applications. Ex vivo study. Med Oral Patol Oral Cir Bucal 2012; 17:e697-704. [PMID: 22322506 PMCID: PMC3476036 DOI: 10.4317/medoral.17726] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/27/2011] [Accepted: 09/27/2011] [Indexed: 01/14/2023] Open
Abstract
Objective: Literature reports bactericidal and biostimulant effects for Nd:YAG laser procedures on bone and oral mucosa but the possible overheating can cause damage to anatomical structures.
The aim of the study is the evaluation of thermal increase in different levels of oral tissues: mucosa, periosteum and bone during defocused application of Nd:YAG laser at different parameters.
Study Design: Superficial thermal evaluation was performed in pig jaws with a thermal camera device; deep thermal evaluation was realized by 4 thermocouples placed at a subperiosteal level and at 1,2 and 4 mm depth in the jaw bone. Laser applications of 1 minute were performed 5 times (with a pause of 1 minute) on a surface of 4 cm2 with a Nd:YAG laser (VSP mode, 320 micrometer fiber, defocused mode) with different parameters. Temperatures were recorded before and after laser applications and after each pause in order to evaluate also the thermal relaxation of tissues.
Results: At submucosal level, mean thermal increase was between 1.1°C and 13.2°C, at 1 mm depth between 1.1°C and 8.5°C, at 2 mm depth between 1.1°C and 6.8°C, at 4 mm depth between 1.0°C and 5.3°C. Temperature decrease during the rest time period was variable between 0°C and 2.5°C.
Conclusions: Temperatures reached during clinical procedures with parameters reported in the literature in biostimulation protocols (1.25-2 Watts) for the five minutes of application are not dangerous for biological structures. The decrease in temperature during the rest time period is less considerable in the bone in comparison to oral mucosa.
Key words:Nd:YAG laser, thermal increase, thermocouple, thermal camera, low level laser therapy.
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Affiliation(s)
- Paolo Vescovi
- Unit of Oral Medicine, Oral Pathology and Laser-Assisted Oral Surgery, Department of ENT-Dental Ophtalmological and Cervico-Facial Sciences, University of Parma, Italy
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