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Selestin Raja I, Kim C, Oh N, Park JH, Hong SW, Kang MS, Mao C, Han DW. Tailoring photobiomodulation to enhance tissue regeneration. Biomaterials 2024; 309:122623. [PMID: 38797121 DOI: 10.1016/j.biomaterials.2024.122623] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2024] [Revised: 04/25/2024] [Accepted: 05/19/2024] [Indexed: 05/29/2024]
Abstract
Photobiomodulation (PBM), the use of biocompatible tissue-penetrating light to interact with intracellular chromophores to modulate the fates of cells and tissues, has emerged as a promising non-invasive approach to enhancing tissue regeneration. Unlike photodynamic or photothermal therapies that require the use of photothermal agents or photosensitizers, PBM treatment does not need external agents. With its non-harmful nature, PBM has demonstrated efficacy in enhancing molecular secretions and cellular functions relevant to tissue regeneration. The utilization of low-level light from various sources in PBM targets cytochrome c oxidase, leading to increased synthesis of adenosine triphosphate, induction of growth factor secretion, activation of signaling pathways, and promotion of direct or indirect gene expression. When integrated with stem cell populations, bioactive molecules or nanoparticles, or biomaterial scaffolds, PBM proves effective in significantly improving tissue regeneration. This review consolidates findings from in vitro, in vivo, and human clinical outcomes of both PBM alone and PBM-combined therapies in tissue regeneration applications. It encompasses the background of PBM invention, optimization of PBM parameters (such as wavelength, irradiation, and exposure time), and understanding of the mechanisms for PBM to enhance tissue regeneration. The comprehensive exploration concludes with insights into future directions and perspectives for the tissue regeneration applications of PBM.
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Affiliation(s)
| | - Chuntae Kim
- Institute of Nano-Bio Convergence, Pusan National University, Busan, 46241, Republic of Korea; Center for Biomaterials Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
| | - Nuri Oh
- Department of Chemistry and Biology, Korea Science Academy of KAIST, Busan, 47162, Republic of Korea
| | - Ji-Ho Park
- Department of Bio and Brain Engineering and KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea
| | - Suck Won Hong
- Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea
| | - Moon Sung Kang
- Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea
| | - Chuanbin Mao
- Department of Biomedical Engineering, The Chinese University of Hong Kong, Sha Tin, Hong Kong SAR, China.
| | - Dong-Wook Han
- Institute of Nano-Bio Convergence, Pusan National University, Busan, 46241, Republic of Korea; Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
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Patil MB, Pai DD, Prakash S. Effect of low-level laser therapy on proliferation and cytotoxicity of mouse fibroblasts and human fibroblasts: An in vitro study. J Indian Soc Periodontol 2024; 28:338-348. [PMID: 39742060 PMCID: PMC11684573 DOI: 10.4103/jisp.jisp_376_23] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/16/2023] [Accepted: 09/20/2024] [Indexed: 01/03/2025] Open
Abstract
Background Diode laser is known for its biostimulatory effects on various cell populations such as osteoblasts and fibroblasts. The usage of low-level laser therapy for photobiomodulation depends on its type, emission wavelength, and energy selected. Previously performed in vitro studies have determined its effect on cell proliferation and cytotoxicity; however, the results were inconsistent. Objectives The objectives of this study were to evaluate and compare diode laser effect on mouse embryonic fibroblasts (MEFs) and human gingival fibroblasts (HGFs) for proliferation and cytotoxicity. Materials and Methods 32 samples, 16 wells of MEF cells (Group I) and HGF cells (Group II) each with 8 subgroups containing control (no laser irradiation) and experimental (laser irradiated) groups were cultured after being seeded at 4000 cells/ well with 4cm inter-well distance. Experimental group cell cultures were irradiated with a single dose of 810 nm diode laser (energy 1J/cm2, 12.5 mW, 80 s/well) using continuous wave mode after 1 day of incubation. Spectrophotometric analysis was done after 24 h of laser irradiation for cell proliferation and cell cytotoxicity on the 2nd, 3rd, and 7th days on all groups. Results Human and mouse fibroblast groups showed positive results when irradiated with an 810 nm laser. A hundred percent proliferation was seen for MEF in 7 days and HGF in 2 days. Two percent cytotoxicity was seen on the 2nd and 3rd day in MEF. Increased cell proliferation and minimal cytotoxic effects were seen with diode laser usage. Conclusion Low-level laser irradiation indicates an increase in cell proliferation with less cytotoxicity on mouse and human fibroblasts.
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Affiliation(s)
- Mallanagouda B. Patil
- Department of Periodontics, College of Dental Sciences, Davanagere, Karnataka, India
| | - Deeksha D. Pai
- Department of Periodontics, College of Dental Sciences, Davanagere, Karnataka, India
| | - Shobha Prakash
- Department of Periodontics, College of Dental Sciences, Davanagere, Karnataka, India
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Wiegand C, Dirksen A, Tittelbach J. Treatment with a red-laser-based wound therapy device exerts positive effects in models of delayed keratinocyte and fibroblast wound healing. PHOTODERMATOLOGY, PHOTOIMMUNOLOGY & PHOTOMEDICINE 2024; 40:e12926. [PMID: 37957888 DOI: 10.1111/phpp.12926] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/21/2023] [Revised: 09/21/2023] [Accepted: 10/31/2023] [Indexed: 11/15/2023]
Abstract
BACKGROUND Light therapy is widely used in medicine. Specifically, photobiomodulation has been shown to exert beneficial effects in wound healing disorders, which present a major challenge in health care. The study's aim was providing information on the effect of a novel, red-laser-based wound therapy device (WTD) on keratinocytes and fibroblasts during wound healing under optimal and non-optimal conditions. METHODS The scratch wound assay was employed as a wound healing model for mechanical damage with readjustment of specific cell milieus, explicitly chronic TH1 inflammation and TH2-dominant conditions. Furthermore, gene expression analysis of pro-inflammatory cytokines (IL1A, IL6, CXCL8), growth factors (TGFB1, PDGFC), transcription factors (NFKB1, TP53) and heat shock proteins (HSP90AA1, HSPA1A, HSPD1) as well as desmogleins (DSG1, DSG3) in keratinocytes and collagen (COL1A1, COL3A1) in fibroblasts was performed after WTD treatment. RESULTS It was shown that WTD treatment is biocompatible and supports scratch wound closure under non-optimal conditions. A distinct enhancement of desmoglein and collagen gene expression as well as induction of early growth factor gene expression was observed under chronic inflammatory conditions. Moreover, WTD increased HSPD1 transcript levels in keratinocytes and augmented collagen expression in fibroblasts during wound healing under TH2 conditions. WTD treatment also alleviated the inflammatory response in keratinocytes and induced early growth factor gene expression in fibroblasts under physiological conditions. CONCLUSION Positive effects described for wound treatment with WTD could be replicated in vitro and seem to be to be conferred by a direct influence on cellular processes taking place in keratinocytes and fibroblasts during wound healing.
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Affiliation(s)
- Cornelia Wiegand
- Department of Dermatology, Jena University Hospital, Friedrich Schiller University, Jena, Germany
| | | | - Jörg Tittelbach
- Department of Dermatology, Jena University Hospital, Friedrich Schiller University, Jena, Germany
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Nowak-Terpiłowska A, Zeyland J, Hryhorowicz M, Śledziński P, Wyganowska M. Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture. Life (Basel) 2023; 13:life13051136. [PMID: 37240781 DOI: 10.3390/life13051136] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2023] [Revised: 04/24/2023] [Accepted: 05/02/2023] [Indexed: 05/28/2023] Open
Abstract
Phototherapy plays a key role in wound healing and tissue regeneration. The use of lasers has the potential to become an effective and minimally invasive treatment in periodontal and peri-implant disease. The aim of this study was to evaluate the influence of three laser wavelengths with the combination of parameters such as power density and energy density on human gingival fibroblasts (hGFs) in vitro culture. Isolated cells were seeded in 96-well plates with culture medium (DMEM, Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum (FBS). After 24 h cells were irradiated (1064, 980 and 635 nm, various energy density value). After 24, 48 and 72 h, cells were evaluated for viability. Data were analyzed by ANOVA followed by Tukey's HSD test. We found the best outcomes for hGFs irradiated with laser 1064 nm for all combinations of power output (50/400/1000 mW) and energy dose (3/25/64 J/cm2) after 48 h and 72 h compared with control group. Cell viability increase ranged from 0.6× (3 J/cm2, 50 mW) to 1.3× (64 J/cm2, 1000 mW). Our findings indicate that the appropriate use of low-level laser irradiation (LLLI) can increase the proliferation rate of cultured cells. The use of LLLI can be extremely useful in tissue engineering and regenerative medicine.
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Affiliation(s)
| | - Joanna Zeyland
- Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 60-632 Poznan, Poland
| | - Magdalena Hryhorowicz
- Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 60-632 Poznan, Poland
| | - Paweł Śledziński
- Department of Genome Engineering, Institute of Bioorganic Chemistry, Polish Academy of Science, 61-704 Poznań, Poland
| | - Marzena Wyganowska
- Department of Dental Surgery, Periodontology and Oral Mucosa Diseases, Poznan University of Medical Sciences, 60-812 Poznan, Poland
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Kasowanjete P, Abrahamse H, Houreld NN. Photobiomodulation at 660 nm Stimulates In Vitro Diabetic Wound Healing via the Ras/MAPK Pathway. Cells 2023; 12:cells12071080. [PMID: 37048153 PMCID: PMC10093328 DOI: 10.3390/cells12071080] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2023] [Revised: 03/23/2023] [Accepted: 03/28/2023] [Indexed: 04/07/2023] Open
Abstract
Diabetic foot ulcers (DFUs) are open chronic wounds that affect diabetic patients due to hyperglycaemia. DFUs are known for their poor response to treatment and frequently require amputation, which may result in premature death. The present study evaluated the effect of photobiomodulation (PBM) at 660 nm on wound healing via activation of Ras/MAPK signalling in diabetic wounded cells in vitro. This study used four human skin fibroblast cell (WS1) models, namely normal (N), wounded (W), diabetic (D), and diabetic wounded (DW). Cells were irradiated at 660 nm with 5 J/cm2. Non-irradiated cells (0 J/cm2) served as controls. Cells were incubated for 24 and 48 h post-irradiation, and the effect of PBM on cellular morphology and migration rate, viability, and proliferation was assessed. Basic fibroblast growth factor (bFGF), its phosphorylated (activated) receptor FGFR, and phosphorylated target proteins (Ras, MEK1/2 and MAPK) were determined by enzyme-linked immunosorbent assay (ELISA) and Western blotting; nuclear translocation of p-MAPK was determined by immunofluorescence. PBM resulted in an increase in bFGF and a subsequent increase in FGFR activation. There was also an increase in downstream proteins, p-Ras, p-MEK1/2 and p-MAPK. PBM at 660 nm led to increased viability, proliferation, and migration as a result of increased bFGF and subsequent activation of the Ras/MAPK signalling pathway. Therefore, this study can conclude that PBM at 660 nm stimulates in vitro diabetic wound healing via the bFGF-activated Ras/MAPK pathway.
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Affiliation(s)
- Patricia Kasowanjete
- Laser Research Centre, University of Johannesburg, Johannesburg 2006, South Africa
| | - Heidi Abrahamse
- Laser Research Centre, University of Johannesburg, Johannesburg 2006, South Africa
| | - Nicolette N. Houreld
- Laser Research Centre, University of Johannesburg, Johannesburg 2006, South Africa
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Kasowanjete P, Houreld NN, Abrahamse H. The effect of photomodulation on fibroblast growth factor and the Ras/MAPK signalling pathway: a review. J Wound Care 2022; 31:832-845. [DOI: 10.12968/jowc.2022.31.10.832] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/17/2023]
Abstract
Objective: Current therapies and technologies used to treat hard-to-heal diabetic wounds are limited to a 50% healing rate. The rise in the percentage of lower limb non-traumatic amputations in patients with diabetes has caused an increased demand for alternative, effective and safe treatment modalities. Photobiomodulation therapy (PBMT) utilises light to induce physiological changes and provide therapeutic benefits and has been shown to increase the healing of hard-to-heal wounds through the release of growth factors. The aim of this narrative review is to investigate the effect of photobiomodulation (PBM) on fibroblast growth factor (FGF) and the role of the Ras/MAPK signalling pathway in diabetic wound healing. Method: Relevant journal articles were obtained through PubMed and Google Scholar. Results: Experimental and clinical findings from the review show that PBM can stimulate the release of growth factors, including FGF, an essential cytokine in wound healing, and one which is present at lower concentrations in diabetic wounds. There is also activation of the Ras/MAPK signalling pathway. Conclusion: One mechanism through which healing may be stimulated by PBM is via the FGF-Ras/MAPK signalling pathway, although strong evidence under hyperglycaemic conditions is lacking.
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Affiliation(s)
| | - Nicolette N Houreld
- Laser Research Centre, University of Johannesburg, Johannesburg, South Africa
| | - Heidi Abrahamse
- Laser Research Centre, University of Johannesburg, Johannesburg, South Africa
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Bakshi PV, Setty SB, Kulkarni MR. Photobiomodulation of human gingival fibroblasts with diode laser - A systematic review. J Indian Soc Periodontol 2022; 26:5-12. [PMID: 35136310 PMCID: PMC8796789 DOI: 10.4103/jisp.jisp_90_21] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/09/2021] [Revised: 09/19/2021] [Accepted: 10/24/2021] [Indexed: 11/21/2022] Open
Abstract
Low-level laser therapy (LLLT) is being extensively studied in the field of periodontics as a noninvasive technique to achieve better results after nonsurgical and surgical therapy. However, there is a lack of definitive guidelines for the use of LLLT to promote gingival and periodontal wound healing. The primary objective of this systematic review was to critically analyze the studies evaluating the effect of low-level diode laser on human gingival fibroblasts in vitro and to develop wavelength-specific guidelines for photobiomodulation of human gingival fibroblasts. A thorough electronic and manual search was conducted for relevant articles published until December 2019. Nine studies were included in the review after the initial screening of 1334 articles. Our data analysis revealed that LLLT with diode laser stimulates human gingival fibroblasts as there was the increase in cell viability, proliferation, migration, and protein synthesis in irradiated cells. The diode lasers in the 600–700 nm spectrum were effective in the 10 mW to 30 mW power range. Lasers in the 700–800 nm range were effective in the 25–50 mW power range and diode lasers in the 800–900 nm range were effective at a power setting of 10 mW. It was possible to ascertain a suitable power setting for a particular wavelength spectrum, but no other parameters could be defined due to a lack of reporting of details. Hence, the authors have developed guidelines for comprehensive reporting of in–vitro studies to facilitate future research and overcome existing lacunae in knowledge.
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Affiliation(s)
- Purva Vijay Bakshi
- Department of Periodontics, SDM College of Dental Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India
| | - Swati Badarinarayan Setty
- Department of Periodontics, SDM College of Dental Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India
| | - Mihir Raghavendra Kulkarni
- Department of Periodontics, SDM College of Dental Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India
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Mármora BC, Brochado FT, Schmidt TR, Santos LG, Araújo AAD, Medeiros CACXD, Ribeiro SB, Martins MAT, Pilar EFS, Wagner VP, Martins MD. Defocused high-power diode laser accelerates skin repair in a murine model through REDOX state modulation and reepithelization and collagen deposition stimulation. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2021; 225:112332. [PMID: 34653929 DOI: 10.1016/j.jphotobiol.2021.112332] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/07/2021] [Revised: 09/08/2021] [Accepted: 10/07/2021] [Indexed: 12/15/2022]
Abstract
Skin wounds represent a burden in healthcare. Our aim was to investigate for the first time the effects of defocused high-power diode laser (DHPL) on skin healing in an animal experimental model and compare it with gold standard low-level laser therapy. Male Wistar rats were divided into 5 groups: Negative control; Sham; 0.1 W laser (L0.1 W); DHPL Dual 1 W (DHPLD1 W); and DHPL Dual 2 W (DHPLD2 W). Rats were euthanized on days 3, 5, 10, 14 and 21. Clinical, morphological, PicroSirus, oxidative stress (MDA, SOD and GSH) and cytokines (IL-1β, IL-10 and TNF-α) analyses were performed. A faster clinical repair was observed in all laser groups at D10 and D14. DHPLD1 W exhibited lower inflammation and better reepithelization compared to other groups at D10. DHPL protocols modulated oxidative stress by decreasing MDA and increasing SOD and GSH. Collagen maturation was triggered by all protocols tested and L0.1 W modulated cytokines release (IL-1β and TNF-α) at D3. In conclusion, DHPL, especially DHPL1 W protocol, accelerated skin healing by triggering reepithelization and collagen maturation and modulating inflammation and oxidative stress.
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Affiliation(s)
- Belkiss Câmara Mármora
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Fernanda Thomé Brochado
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Tuany Rafaelli Schmidt
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Lucas Gonçalves Santos
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Aurigena Antunes de Araújo
- Post-Graduation Program Oral Science, Post-Graduation Program in Pharmaceutical Science, Department of Biophysics and Pharmacology, Federal University of Rio Grande do Norte, Natal, Brazil
| | - Caroline Addison Carvalho Xavier de Medeiros
- Department of Biophysics and Pharmacology, Postgraduate Program in Biological Science and Rede Nordeste de Biotecnologia, Renorbio, Federal University of Rio Grande Norte, Natal, Brazil
| | - Susana Barbosa Ribeiro
- Postgraduate Program in Biological Science and Rede Nordeste de Biotecnologia, Renorbio, Federal University of Rio Grande Norte, Natal, Brazil
| | - Marco Antonio Trevizani Martins
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil; Department of Oral Medicine, Hospital de Clínicas de Porto Alegre (HCPA/UFRGS), Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Emily Ferreira Salles Pilar
- Experimental Pathology Unit, Clinics Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Vivian Petersen Wagner
- Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil
| | - Manoela Domingues Martins
- Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil; Department of Oral Medicine, Hospital de Clínicas de Porto Alegre (HCPA/UFRGS), Federal University of Rio Grande do Sul, Porto Alegre, Brazil; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.
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Etemadi A, Imani N, Seyed Jafari E, Chiniforush N. In Vitro Effect of Photodynamic Therapy with Indocyanine Green Followed by 660 nm Photobiomodulation Therapy on Fibroblast Viability. Photochem Photobiol 2021; 98:498-503. [PMID: 34553775 DOI: 10.1111/php.13524] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/11/2021] [Accepted: 09/20/2021] [Indexed: 11/29/2022]
Abstract
This in␣vitro study sought to assess the effect of repetitive PBMT on the viability of fibroblasts following aPDT with indocyanine green (ICG). In this in␣vitro experimental study, human gingival fibroblasts (HGFs) were obtained and incubated in a culture medium. After reaching 10 000 cells cm-2 , the cells were divided into five groups of control, aPDT with ICG and 808 nm (energy density of 24 J cm-2 ), PBMT immediately after aPDT, PBMT with 660 nm (energy density of 7.2 J cm-2 ) immediately and 24 h after aPDT and PBMT immediately and 24 and 48 h after aPDT in 48-well plates. Cell viability was evaluated using the methyl thiazolyl tetrazolium (MTT) assay after 1, 4 and 7 days of incubation. Statistical analyses were performed using one-way ANOVA. Cell viability significantly decreased in group 2 (P < 0.002). We observed no significant increase in cell viability at any time point in group 3 (P > 0.05). Cell viability significantly increased in groups 4 and 5 after the first day of incubation (P < 0.000). Emission of 660 nm as PBMT for two and three times along with passage of time would increase the viability of HGFs following aPDT with ICG.
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Affiliation(s)
- Ardavan Etemadi
- Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | - Negar Imani
- Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | | | - Nasim Chiniforush
- Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
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10
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Etemadi A, Sadatmansouri S, Sodeif F, Jalalishirazi F, Chiniforush N. Photobiomodulation Effect of Different Diode Wavelengths on the Proliferation of Human Gingival Fibroblast Cells. Photochem Photobiol 2021; 97:1123-1128. [PMID: 34107547 DOI: 10.1111/php.13463] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/26/2021] [Revised: 05/16/2021] [Accepted: 06/02/2021] [Indexed: 12/01/2022]
Abstract
This study is focused on comparing the effect of various energy densities and wavelengths of diode lasers on the proliferation of human gingival fibroblast (HGF) cells in vitro. In this study, 204 sample cells were examined in 4 test groups (laser radiation) and 1 control group (non-laser radiation). The proliferation rate of radiated cells with wavelengths of 635, 660, 808 and 980 nm and the densities of 1, 1.5, 2.5 and 4 J cm-2 was measured after 1, 3 and 5 days using the MTT assay. The proliferation rate of human gingival fibroblast (HGF) cells in test groups was increased on day 1 at wavelengths of 635, 808 and 980 nm and on day 3 at the wavelength of 980 nm compared with the control group. Our findings denoted that the photobiomodulation therapy increased the proliferation rate of HGF. The most desirable laser radiation setting, which led to the highest proliferation rate of the cells, included 980 nm wavelength with 1, 1.5 and 4 J cm-2 energy densities and 635 nm wavelength with 4 J cm-2 energy density.
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Affiliation(s)
- Ardavan Etemadi
- Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.,Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
| | - Saeed Sadatmansouri
- Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | - Fatemeh Sodeif
- Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
| | - Fatemeh Jalalishirazi
- Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Nasim Chiniforush
- Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.,Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, Italy
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11
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In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration. Int J Mol Sci 2020; 21:ijms21239002. [PMID: 33256246 PMCID: PMC7730548 DOI: 10.3390/ijms21239002] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/10/2020] [Revised: 11/18/2020] [Accepted: 11/21/2020] [Indexed: 02/06/2023] Open
Abstract
Periodontal disease is a chronic inflammatory disease caused by periodontal bacteria. Recently, periodontal phototherapy, treatment using various types of lasers, has attracted attention. Photobiomodulation, the biological effect of low-power laser irradiation, has been widely studied. Although many types of lasers are applied in periodontal phototherapy, molecular biological effects of laser irradiation on cells in periodontal tissues are unclear. Here, we have summarized the molecular biological effects of diode, Nd:YAG, Er:YAG, Er,Cr:YSGG, and CO2 lasers irradiation on cells in periodontal tissues. Photobiomodulation by laser irradiation enhanced cell proliferation and calcification in osteoblasts with altering gene expression. Positive effects were observed in fibroblasts on the proliferation, migration, and secretion of chemokines/cytokines. Laser irradiation suppressed gene expression related to inflammation in osteoblasts, fibroblasts, human periodontal ligament cells (hPDLCs), and endothelial cells. Furthermore, recent studies have revealed that laser irradiation affects cell differentiation in hPDLCs and stem cells. Additionally, some studies have also investigated the effects of laser irradiation on endothelial cells, cementoblasts, epithelial cells, osteoclasts, and osteocytes. The appropriate irradiation power was different for each laser apparatus and targeted cells. Thus, through this review, we tried to shed light on basic research that would ultimately lead to clinical application of periodontal phototherapy in the future.
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do Valle IB, Prazeres PHDM, Mesquita RA, Silva TA, de Castro Oliveira HM, Castro PR, Freitas IDP, Oliveira SR, Gomes NA, de Oliveira RF, Marquiore LF, Macari S, do Amaral FA, Jácome-Santos H, Barcelos LS, Menezes GB, Marques MM, Birbrair A, Diniz IMA. Photobiomodulation drives pericyte mobilization towards skin regeneration. Sci Rep 2020; 10:19257. [PMID: 33159113 PMCID: PMC7648092 DOI: 10.1038/s41598-020-76243-7] [Citation(s) in RCA: 21] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/16/2020] [Accepted: 10/26/2020] [Indexed: 12/11/2022] Open
Abstract
Photobiomodulation is being widely applied for improving dermal or mucosal wound healing. However, the underlying cellular and molecular processes that directly contribute to its effects remain poorly understood. Pericytes are relevant cells involved in the wound microenvironment and could be one of the main targets of photobiomodulation due to their plasticity and perivascular localization. Herein, we investigate tissue repair under the photobiomodulation stimulus using a pericyte labeled (or reporter) transgenic mice. Using a model of two contralateral back wounds, one the control and the other photoactivated daily (660 nm, 20 mW, 0.71 W/cm2, 5 J/cm2, 7 s, 0.14 J), we showed an overall influx of immune and undifferentiated cells and higher mobilization of a potent pericyte subpopulation (Type-2 pericytes) in the photoactivated wounds in comparison to the controls. Doppler analysis showed a significant increase in the blood flow in the photoactivated wounds, while marked vascular supply was observed histologically. Histochemical analysis has indicated more advanced stages of tissue repair after photoactivation. These data suggest that photobiomodulation significantly accelerates tissue repair through its vascular effects with direct recruitment of pericytes to the injury site.
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Affiliation(s)
- Isabella Bittencourt do Valle
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
- Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | | | - Ricardo Alves Mesquita
- Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Tarcília Aparecida Silva
- Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | | | - Pollyana Ribeiro Castro
- Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Iuri Dornelas Prates Freitas
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
- School of Dentistry, Faculdade Sete Lagoas, Sete Lagoas, Minas Gerais, Brazil
| | - Sicília Rezende Oliveira
- Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Natália Aparecida Gomes
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
| | - Rafaela Férrer de Oliveira
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
| | - Larissa Fassarela Marquiore
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
| | - Soraia Macari
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
| | - Flávio Almeida do Amaral
- Department of Biochemistry and Immunology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Humberto Jácome-Santos
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil
- Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Lucíola Silva Barcelos
- Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Gustavo Batista Menezes
- Department of Morphology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | | | - Alexander Birbrair
- Departament of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
| | - Ivana Márcia Alves Diniz
- Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31.270-901, Brazil.
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Alves FAM, Marques MM, Cavalcanti SCSXB, Pedroni ACF, Ferraz EP, Miniello TG, Moreira MS, Jerônimo T, Deboni MCZ, Lascala CA. Photobiomodulation as adjunctive therapy for guided bone regeneration. A microCT study in osteoporotic rat model. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2020; 213:112053. [PMID: 33142216 DOI: 10.1016/j.jphotobiol.2020.112053] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/06/2020] [Revised: 10/01/2020] [Accepted: 10/06/2020] [Indexed: 02/02/2023]
Abstract
Regeneration of diseased bone is challenging. Guided bone regeneration (GBR) has been applied to favor the bone repair. Photobiomodulation (PBM) is also a recognized therapy able to improve bone repair in healthy and diseased individuals. Thus, with the hypothesis that PBM therapy could improve the GBR of diseased bone, this study evaluated the effect of PBM as adjunctive therapy to GBR in osteoporotic rats. Osteoporosis was induced in rats using the oophorectomy model. Then, 5-mm calvaria bone defects were created and treated according to the experimental groups, as follows: with no further treatment (Control); conventional GBR (Membrane), GBR and PBM applied with 3 s, 4 J/cm2 and 0.12 J per point (PBM-1) and GBR and PBM applied with 10s, 14 J/cm2, 0.4 J per point (PBM-2). PBM therapy (808 nm, 40 mW, 1.42 W/cm2) was applied immediately, 48 and 96 h postoperatively. Four and eight weeks later, the samples were harvested and processed for micro-computerized tomography (Micro CT). Data were statistically compared (p < 0.05). From 4 to 8 weeks mostly significant changes were observed in the PBM groups. The bone volume fraction and number of trabeculae of the PBM groups, especially the PBM-1, were significantly higher than those of Control (p < 0.0001). The values of thickness and separation of the trabeculae and structural model index of the PBM groups were significantly smaller than Control (p < 0.0001). The connectivity density was significantly higher on Membrane and PBM groups than Control (p < 0.0004). The application of PBM as adjunctive therapy to GBR results in enhanced bone formation and maturation in comparison to the conventional GBR in the regeneration of lesions of osteoporotic bone in rats. Overviewing the challenges that face bone regeneration in patients with osteoporosis, our findings open new perspectives on the treatment of bone defects under osteoporotic conditions.
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Affiliation(s)
| | | | | | | | | | | | | | - Tomaís Jerônimo
- Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil
| | | | - Cesar Angelo Lascala
- Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
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Cardoso MV, do Vale Placa R, Sant'Ana ACP, Greghi SLA, Zangrando MSR, de Rezende MLR, Oliveira RC, Damante CA. Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique. Lasers Med Sci 2020; 36:541-553. [PMID: 32514865 DOI: 10.1007/s10103-020-03056-5] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/24/2019] [Accepted: 06/01/2020] [Indexed: 12/16/2022]
Abstract
The purposes of this study are to evaluate the effects of photobiomodulation (PBM) with laser and LED on rat calvaria osteoblasts (rGO lineage), cultured in osteogenic (OST) or regular (REG) medium, after induction of a quiescent state and to test if PBM is capable of osteogenic induction and if there is a sum of effects when combining OST medium with PBM. Before irradiation, the cells were put in a quiescent state (1% FBS) 24 h, when red (AlGaInP-660 nm) and infrared laser (GaAlAs-808 nm) and LED (637 ± 15 nm) were applied. The groups were as follows: red laser (RL3-5 J/cm2, 3 s and RL5-8.3 J/cm2, 5 s, 1.66 W/cm2); infrared laser (IrL3-5 J/cm2, 3 s and IrL5-8.3 J/cm2, 5 s); LED (LED3-3 s and LED5-5 s, 0.02 J/cm2, 0.885 W/cm2); positive (C+, 10% FBS) and negative control (C-, 1% FBS). For alkaline phosphatase (ALP) and mineralization assays, the cells were cultured in REG (DMEM 10% FBS) and OST medium (DMEM 10% FBS, 50 μg/mL ascorbic acid, 10 mM β-glycerophosphate). Statistical analysis was performed using ANOVA and Tukey's tests (p < 0.05). RL5 and LED5 increased proliferation, in vitro wound closure, ALP, and mineralization in rGO cells (p < 0.05). PBM with red laser and LED induced mineralization by itself, without osteogenic medium, not observed for infrared laser (p < 0.05). A sum of effects was observed in osteogenic medium and PBM by infrared, red laser, and LED (5 s). Red laser and LED increased proliferation, migration, and secretory phases in rGO cells in a dose-dependent manner. PBM with red laser and LED promotes osteogenic induction by itself. PBM with infrared laser and osteogenic medium potentializes mineralization.
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Affiliation(s)
- Matheus Völz Cardoso
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil.
| | - Rebeca do Vale Placa
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil
| | | | - Sebastião Luiz Aguiar Greghi
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil
| | | | - Maria Lucia Rubo de Rezende
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil
| | - Rodrigo Cardoso Oliveira
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil
| | - Carla Andreotti Damante
- Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil
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Vitor LLR, Bergamo MTOP, Lourenço-Neto N, Sakai VT, Oliveira RC, Cruvinel T, Rios D, Garlet GP, Santos CF, Machado MAAM, Oliveira TM. Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells. Clin Oral Investig 2020; 24:4343-4354. [PMID: 32333178 DOI: 10.1007/s00784-020-03298-1] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/26/2019] [Accepted: 04/17/2020] [Indexed: 11/29/2022]
Abstract
OBJECTIVE To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs). MATERIAL AND METHODS HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm2 and 3.7 J/cm2. The control group was not irradiated. MTT, crystal violet, and ELISA assays respectively verified viability, proliferation, and angiogenic protein (supernatant/lysate) at 6 h, 12 h, and 24 h after photobiomodulation. Capillary-like structure formation assay verified functional role. Two-way ANOVA/Tukey's test and ANOVA/Bonferroni's multiple comparisons test respectively verified cell viability/proliferation and intragroup and intergroup comparisons of protein synthesis (p < 0.05). RESULTS Irradiated and non-irradiated HPFs showed statistically similar cell viability and proliferation pattern. Intragroup comparisons showed similar patterns of protein synthesis for all groups: VEGF-A, VEGF-C, and vascular endothelial growth factor receptor 1 (VEGFR1) increased significantly in the supernatant, while FGF-2 and VEGF-A increased significantly in the lysate. The lower fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (6 h and 12 h) and VEGF-D (24 h) in the lysate, while the higher fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (12 h) in the lysate. Regardless of the time, both fluences statistically downregulated placental growth factor (PLGF) and PDGF secretion. Both fluences statistically decreased VEGF-A secretion (24 h) and PLGF production (6 h). CONCLUSION Photobiomodulation produced stimulatory effects on angiogenic protein secretion by pulp fibroblasts. In terms of photobiomodulation, over time, both fluences significantly increased the secretion of VEGF-A, VEGF-C, and VEGFR1 and significantly upregulated BMP-9 (6 h) in the supernatant; for capillary-like structure formation, the fluence of 2.5 J/cm2 was better than the fluence of 3.7 J/cm2. CLINICAL RELEVANCE This study results addressed effective photobiomodulation parameters tailored for pulp angiogenesis.
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Affiliation(s)
- Luciana Lourenço Ribeiro Vitor
- Department of Pediatric Dentistry, University of Sacred Heart, Rua Irmã Arminda, 10-50, Bauru, São Paulo, 17011-160, Brazil.
| | | | - Natalino Lourenço-Neto
- Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | - Vivien Thiemy Sakai
- Department of Clinics and Surgery, School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil
| | - Rodrigo Cardoso Oliveira
- Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | - Thiago Cruvinel
- Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | - Daniela Rios
- Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | - Gustavo Pompermaier Garlet
- Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | - Carlos Ferreira Santos
- Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
| | | | - Thais Marchini Oliveira
- Department of Pediatric Dentistry, Orthodontics, and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil
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Abstract
Diabetic patients frequently develop chronic ulcers of the lower extremities, which are a frequent cause for hospitalization and amputation, placing strain on patients, their families, and healthcare systems. Present therapies remain a challenge, with high recurrence rates. Photobiomodulation (PBM), which is the non-invasive application of light at specific wavelengths, has been shown to speed up healing of chronic wounds, including diabetic foot ulcers (DFUs). PBM produces photophysical and photochemical changes within cells without eliciting thermal damage. It has been shown to promote tissue regeneration and speed up wound repair by reducing inflammation and oxidative stress, accelerating cell migration and proliferation, and promoting extracellular matrix production and release of essential growth factors. The shortage of rigorous, well-designed clinical trials makes it challenging to assess the scientific impact of PBM on DFUs, and lack of understanding of the underlying mechanisms also hinders the conventional use of this therapy. This review gives a glimpse into diabetic wound healing and PBM, and the effects of PBM on diabetic wound healing.
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Illescas-Montes R, Melguizo-Rodríguez L, García-Martínez O, de Luna-Bertos E, Manzano-Moreno FJ, Ruiz C, Ramos-Torrecillas J. Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser. Sci Rep 2019; 9:12037. [PMID: 31427686 PMCID: PMC6700136 DOI: 10.1038/s41598-019-48595-2] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/26/2018] [Accepted: 08/08/2019] [Indexed: 12/26/2022] Open
Abstract
Low-Level Laser Therapy is used as regenerative therapy in different clinical fields. This is due to its photobiomodulation effect via cell signaling on different cell populations, Including fibroblasts, cells involved in tissue regeneration and healing. The aim was to analyze the effect of 940 nm diode laser on the gene expression of different markers involved in fibroblast growth, differentiation, and migration. Real-time polymerase chain reaction (q-RT-PCR) was used to quantify the expression of fibroblast growth factor (FGF), connective tissue growth factor (CTGF), vascular-endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), TGFβ-receptors (TGFβR1, TGFβR2, and TGFβR3), discoidin-domain receptor-2 (DDR2), matrix metalloproteinase-2 (MMP2), α-actin, fibronectin, decorin, and elastin on human fibroblast, treated with single dose (T1) or two doses (T2) of diode laser at 0.5 Watts and 4 J/cm2. A significant increase in the expression of FGF, TGF-β1, TGFβR1, TGFβR2, α-actin, fibronectin, decorin, DDR2 and MMP2 was observed after both treatments. A decrease was observed in expression of elastin (T1 and T2), and CTGF (T2). These changes underlie the biostimulatory effect of laser on fibroblasts, which translates into an increase in short-term proliferation and in long-term differentiation to myofibroblasts. These data support the therapeutic potential of diode laser for wound repair.
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Affiliation(s)
- Rebeca Illescas-Montes
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain
| | - Lucía Melguizo-Rodríguez
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain
| | - Olga García-Martínez
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain
| | - Elvira de Luna-Bertos
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain
| | - Francisco Javier Manzano-Moreno
- Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain.,Biomedical Group (BIO277). Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain
| | - Concepción Ruiz
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain. .,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain. .,Institute of Neuroscience, University of Granada, Centro de Investigación Biomédica (CIBM). Parque de Tecnológico de la Salud (PTS), Granada, Spain.
| | - Javier Ramos-Torrecillas
- Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.,Instituto Investigación Biosanitaria ibs.Granada, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, Granada, 18012, Spain
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18
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Root surface demineralization by citric acid/tetracycline gel and aPDT associated to subepithelial connective tissue graft improves root coverage outcomes. A 12-month preliminary randomized clinical trial. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2019; 197:111528. [DOI: 10.1016/j.jphotobiol.2019.111528] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/12/2018] [Revised: 03/14/2019] [Accepted: 06/03/2019] [Indexed: 01/19/2023]
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19
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Effect of single and multiple doses of low-level laser therapy on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). Lasers Med Sci 2019; 34:1917-1924. [PMID: 31267320 DOI: 10.1007/s10103-019-02836-y] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/18/2018] [Accepted: 06/25/2019] [Indexed: 01/09/2023]
Abstract
The present study aimed to evaluate in vitro whether the low-level laser (LLL) delivering fractionated total energy (multiple irradiation) or single irradiation stimulates regeneration-associated events (viability and proliferation) in stem cells from human exfoliated deciduous teeth (SHED). Cells received LLL irradiation (InGaAlP-660 nm), according to the following experimental groups: G1 (single irradiation 2.5 J/cm2, 10 mW, 10 s, 0.10 J), G2 (single irradiation 5.0 J/cm2, 10 mW, 20 s, 0.20 J), G3 (single irradiation 7.5 J/cm2, 10 mW, 30 s, 0.30 J), G4 (two irradiations 2.5 J/cm2, 10 mW, 10 s; total energy 0.20 J), G5 (three irradiations 2.5 J/cm2, 10 mW, 10 s; total energy 0.30 J), and G6 (non-irradiated). Cell viability was assessed by MTT and trypan blue exclusion (TBE) methods, while cell proliferation was evaluated by crystal violet (CV) and sulforhodamine B (SRB) assays after 24, 48, and 72 h after the first irradiation. By MTT, there was no difference between groups at 24 and 72 h. At 48 h, the groups subjected to multiple irradiation (G4 and G5) presented higher cell viability rates. The average percentages of viable cells for all groups by TBE method were 91.04%, 96.63%, and 97.48% at 24, 48, and 72 h, respectively. By CV, there was no significant difference between groups at 24 and 48 h; at 72 h, G2, G3, and G4 presented higher cell proliferation. By SRB, G1 and G4 presented lower proliferation rates in all the periods. When the groups presenting the same total energy were compared, G2 (0.20 J) presented lower cell viability rates and higher cell proliferation rates in comparison with G4; G3 (0.30 J) presented similar results to those of G5, with higher cell viability and proliferation. The application of laser delivering fractionated total energy (two or three applications of 2.5 J/cm2) induced higher cell viability at 48 h, while the single irradiation with 2.5 J/cm2 did not stimulate metabolic activity in such period and the proliferation over time. The 5.0 and 7.5 J/cm2 single doses and the three applications of 2.5 J/cm2 maintained cell viability and stimulated proliferation of SHED at 72 h.
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Garrido P, Pedroni A, Cury D, Moreira M, Rosin F, Sarra G, Marques M. Effects of photobiomodulation therapy on the extracellular matrix of human dental pulp cell sheets. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2019; 194:149-157. [DOI: 10.1016/j.jphotobiol.2019.03.017] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/08/2018] [Revised: 12/19/2018] [Accepted: 03/25/2019] [Indexed: 12/11/2022]
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Lemes CHJ, da Rosa WLDO, Sonego CL, Lemes BJ, Moraes RR, da Silva AF. Does laser therapy improve the wound healing process after tooth extraction? A systematic review. Wound Repair Regen 2018; 27:102-113. [PMID: 30324666 DOI: 10.1111/wrr.12678] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/14/2018] [Revised: 05/23/2018] [Accepted: 10/08/2018] [Indexed: 01/18/2023]
Abstract
Laser therapy could amplify the oral wound healing process by stimulating cell regeneration after injury, attenuating pain, and modulating the immune system. The purpose of this systematic review was to evaluate whether the application of laser therapy improved alveolar healing after tooth extractions. Eight electronic databases were screened: MedLine (PubMed), The Cochrane Library, Web of Science, Scopus, Lilacs, Ibecs, Scielo, and BBO. Three reviewers independently assessed the title and abstracts of potentially relevant studies. Only clinical trials and animal experiments that evaluated the wound healing effect of laser therapy after tooth extraction were included. A total of 16 studies fulfilled all criteria, thus 8 animal experiments and 8 clinical trials were included. Different types of laser were evaluated, such as CO2 , GaAlAs, Nd:YAG, Diode Laser, HeNe, and High-frequency Pulsed Diode Laser. Although HF, Diode and GaAs lasers were able to enhance wound healing process in clinical studies, four trials and one animal experiment showed no improvement in wound healing with laser therapy after tooth extractions. In general, the current available evidence in the literature showed that laser therapy improved the wound healing process, but these findings were limited to the type of laser applied and its specific settings. Further well-designed and randomized controlled trials are needed to support a benefit effect of using laser therapy after tooth extraction. PROSPERO registration number: CRD42014007509 (2014).
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Affiliation(s)
| | | | - Camila Leal Sonego
- Department of Buco-maxillofacial Surgery, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
| | | | - Rafael Ratto Moraes
- Department of Restorative Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
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Brassolatti P, de Andrade ALM, Bossini PS, Orth DL, Duarte FO, dos Anjos Souza AB, Parizotto NA, de Freitas Anibal F. Photobiomodulation on critical bone defects of rat calvaria: a systematic review. Lasers Med Sci 2018; 33:1841-1848. [DOI: 10.1007/s10103-018-2653-z] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/16/2018] [Accepted: 09/27/2018] [Indexed: 11/29/2022]
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23
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A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED. Lasers Med Sci 2018; 34:465-471. [DOI: 10.1007/s10103-018-2615-5] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/30/2018] [Accepted: 08/09/2018] [Indexed: 01/01/2023]
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Stein S, Schauseil M, Hellak A, Korbmacher-Steiner H, Braun A. Influence of Photobiomodulation Therapy on Gingivitis Induced by Multi-Bracket Appliances: A Split-Mouth Randomized Controlled Trial. Photomed Laser Surg 2018; 36:399-405. [PMID: 29775553 DOI: 10.1089/pho.2017.4404] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022] Open
Abstract
OBJECTIVE The objective of this split-mouth trial was to investigate the influence of photobiomodulation therapy (PBMT) on adjuvant treatment of gingivitis induced by multi-bracket appliances, after bracket debonding and professional tooth cleaning. MATERIALS AND METHODS Thirteen patients (mean age 16.15 years; standard deviation ±2.12 years) who had completed active orthodontic treatment with fixed orthodontic appliances in an orthodontic clinic were included on a randomized basis. At time point T0, after bracket debonding and professional tooth cleaning, the papilla bleeding index (PBI) and bleeding on probing (BOP) were assessed in the upper jaw by the blinded investigator (M.S.), who was not aware at any time of which quadrant received PBMT. The study was based on a patient-blinded split-mouth design. In each patient, PBMT was administered by a practitioner (S.S.) in one upper quadrant (wavelength: 660 nm; Power: 100 mW; Power density: 100 mW/cm2; Energy density per application point = 2 J/cm2; Energy per application point = 2 J; Total dose = 52 J/cm2; Total energy = 52 J; Irradiation time: 26 × 20 sec), while the other upper quadrant received a simulated laser application with the laser system turned off. Randomized equal allocation of the sides was accomplished. The second PBI and BOP assessment followed 4-6 days after laser irradiation (T1) by M.S. RESULTS No statistical differences were observed between the sides with regard to PBI and BOP values at T0 (p > 0.05). The PBI and BOP values decreased significantly between T0 and T1 on both sides (p < 0.05). At T1, the PBI and BOP values were significantly lower in the laser side in comparison with the control side (p < 0.05). CONCLUSIONS On the basis of these results and study parameters, adjuvant PBMT is able to accelerate the healing process in patients with gingivitis induced by multi-bracket appliances.
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Affiliation(s)
- Steffen Stein
- 1 Department of Orthodontics, University of Marburg , Marburg, Germany
| | - Michael Schauseil
- 1 Department of Orthodontics, University of Marburg , Marburg, Germany
| | - Andreas Hellak
- 1 Department of Orthodontics, University of Marburg , Marburg, Germany
| | | | - Andreas Braun
- 2 Department of Operative Dentistry and Endodontology, University of Marburg , Marburg, Germany
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Soares ASLS, Scelza MZ, Spoladore J, Gallito MA, Oliveira F, Moraes RDCM, Alves GG. Comparison of primary human gingival fibroblasts from an older and a young donor on the evaluation of cytotoxicity of denture adhesives. J Appl Oral Sci 2018; 26:e20160594. [PMID: 29489936 PMCID: PMC5829547 DOI: 10.1590/1678-7757-2016-0594] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/02/2016] [Accepted: 09/14/2017] [Indexed: 01/13/2023] Open
Abstract
Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age.
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Affiliation(s)
- Aline S L Santoro Soares
- Laboratório Experimental de Cultura Celular, Faculdade de Odontologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil
| | - Miriam Zaccaro Scelza
- Laboratório Experimental de Cultura Celular, Faculdade de Odontologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil
| | - Janaína Spoladore
- Unidade de Pesquisa Clínica, Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil
| | - Marcos Antônio Gallito
- Faculdade de Odontologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil
| | - Felipe Oliveira
- Laboratório Experimental de Cultura Celular, Faculdade de Odontologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brasil
| | | | - Gutemberg Gomes Alves
- Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminenes, Niterói, Rio de Janeiro, Brasil
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26
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Asl RM, Ghoraeian P, Monzavi A, Bahador A. Analysis of gene expression of basic fibroblast growth factor (bFGF) following photodynamic therapy in human gingival fibroblasts. Photodiagnosis Photodyn Ther 2017; 20:144-147. [DOI: 10.1016/j.pdpdt.2017.09.010] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2017] [Revised: 09/06/2017] [Accepted: 09/17/2017] [Indexed: 12/12/2022]
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27
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Marques NCT, Neto NL, Prado MTO, Vitor LLR, Oliveira RC, Sakai VT, Santos CF, Machado MAAM, Oliveira TM. Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers Med Sci 2017; 32:1621-1628. [DOI: 10.1007/s10103-017-2301-z] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2017] [Accepted: 08/03/2017] [Indexed: 02/06/2023]
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28
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Sağlam M, Köseoğlu S, Pekbağrıyanık T, Savran L, Enhoş Ş. Effects of high power-pulsed Nd:YAG laser irradiation on the release of transforming growth factor-beta (TGF-β) and vascular endothelial growth factor (VEGF) from human gingival fibroblasts. J COSMET LASER THER 2017; 19:469-474. [PMID: 28665155 DOI: 10.1080/14764172.2017.1342042] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]
Abstract
The purpose of this study was to investigate the effect of different high-power energy settings of a neodymium:yttrium-aluminum-garnet (Nd:YAG) laser (1064 nm) on cell viability of human gingival fibroblasts (GFs) and release of transforming growth factor-beta (TGF-β) and vascular endothelial growth factor (VEGF) on these cells. GFs were isolated from human gingival connective tissues during the crown lengthening procedure. GFs were irradiated with different laser parameters as follows: group 1: 1 W (100 mJ, 10 Hz) 10 seconds; group 2: 1.5 W (150 mJ, 10 Hz) 10 seconds; group 3: 2 W (200 mJ, 10 Hz) 10 seconds; group 4: 1 W (100 mJ, 10 Hz) 20 seconds; group 5: 1.5 W (150 mJ, 10 Hz) 20 seconds; and group 6: 2 W (200 mJ, 10 Hz) 20 seconds. Cell viability/cell proliferation was analyzed with XTT (tetrazolium salt, cell proliferation kit) staining. The release levels of TGF-β and VEGF were analyzed by the enzyme-linked immunosorbent assay. No significant differences were observed in the different laser irradiation groups compared to the control group in terms of cell viability (p > 0.05). The release of TGF-β was not affected by different laser irradiation settings (p > 0.05). Only group 6 promoted significantly higher VEGF release from GFs in 24 hours compared to the control group (p ˂ 0.05). These findings suggest that high-power Nd:YAG laser is probably safe but has a very limited effect for wound healing.
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Affiliation(s)
- Mehmet Sağlam
- a Department of Periodontology, Faculty of Dentistry , Izmir Katip Celebi University , Izmir , Turkey
| | - Serhat Köseoğlu
- a Department of Periodontology, Faculty of Dentistry , Izmir Katip Celebi University , Izmir , Turkey
| | - Tuğba Pekbağrıyanık
- a Department of Periodontology, Faculty of Dentistry , Izmir Katip Celebi University , Izmir , Turkey
| | - Levent Savran
- a Department of Periodontology, Faculty of Dentistry , Izmir Katip Celebi University , Izmir , Turkey
| | - Şükrü Enhoş
- a Department of Periodontology, Faculty of Dentistry , Izmir Katip Celebi University , Izmir , Turkey
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29
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Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature. ACTA ACUST UNITED AC 2017. [DOI: 10.1007/s41547-017-0002-3] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/25/2022]
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30
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Santamaria MP, Fernandes-Dias SB, Araújo CF, Lucas da Silva Neves F, Mathias IF, Rebelato Bechara Andere NM, Neves Jardini MA. 2-Year Assessment of Tissue Biostimulation With Low-Level Laser on the Outcomes of Connective Tissue Graft in the Treatment of Single Gingival Recession: A Randomized Clinical Trial. J Periodontol 2017; 88:320-328. [DOI: 10.1902/jop.2016.160391] [Citation(s) in RCA: 19] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/24/2022]
Affiliation(s)
- Mauro Pedrine Santamaria
- Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, São José dos Campos, Brazil
| | - Stephanie Botti Fernandes-Dias
- Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, São José dos Campos, Brazil
| | - Cassia Fernandes Araújo
- Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, São José dos Campos, Brazil
| | - Felipe Lucas da Silva Neves
- Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, São José dos Campos, Brazil
| | | | | | - Maria Aparecida Neves Jardini
- Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University - UNESP, São José dos Campos, Brazil
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31
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Souza DM, Alves PM, Silva MLF, Paulino TP, Coraspe HO, Mendonça MMS, Ribeiro BM, da Silva MV, Rodrigues Júnior V, Rodrigues DBR. 5-ALA-mediated photodynamic therapy reduces the parasite load in mice infected withLeishmania braziliensis. Parasite Immunol 2017; 39. [DOI: 10.1111/pim.12403] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2016] [Accepted: 11/17/2016] [Indexed: 11/30/2022]
Affiliation(s)
- D. M. Souza
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - P. M. Alves
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - M. L. F. Silva
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - T. P. Paulino
- Cefores - Centro de Educação Profissional; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - H. O. Coraspe
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - M. M. S. Mendonça
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - B. M. Ribeiro
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - M. V. da Silva
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - V. Rodrigues Júnior
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
| | - D. B. R. Rodrigues
- Immunology Laboratory; Department of Biological Sciences; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
- Cefores - Centro de Educação Profissional; Federal University of Triângulo Mineiro; Uberaba Minas Gerais Brazil
- Department of Immunology and Molecular Biology; University of Uberaba; Uberaba Minas Gerais Brazil
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32
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de Almeida JM, de Moraes RO, Gusman DJR, Faleiros PL, Nagata MJH, Garcia VG, Theodoro LH, Bosco AF. Influence of low-level laser therapy on the healing process of autogenous bone block grafts in the jaws of systemically nicotine-modified rats: A histomorphometric study. Arch Oral Biol 2017; 75:21-30. [DOI: 10.1016/j.archoralbio.2016.12.003] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2016] [Revised: 10/21/2016] [Accepted: 12/01/2016] [Indexed: 10/20/2022]
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33
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In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2017; 166:180-186. [DOI: 10.1016/j.jphotobiol.2016.11.022] [Citation(s) in RCA: 19] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/07/2016] [Revised: 10/24/2016] [Accepted: 11/25/2016] [Indexed: 12/13/2022]
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34
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Fernandes AP, Junqueira MDA, Marques NCT, Machado MAAM, Santos CF, Oliveira TM, Sakai VT. Effects of low-level laser therapy on stem cells from human exfoliated deciduous teeth. J Appl Oral Sci 2016; 24:332-7. [PMID: 27556203 PMCID: PMC4990361 DOI: 10.1590/1678-775720150275] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/12/2015] [Accepted: 03/01/2016] [Indexed: 12/13/2022] Open
Abstract
Low-Level Laser Therapy stimulates the proliferation of a variety of types of cells. However, very little is known about its effect on stem cells from human exfoliated deciduous teeth (SHED).
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Affiliation(s)
- Ana Paula Fernandes
- - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Odontopediatria, Ortodontia e Saúde Coletiva, Bauru, SP, Brasil
| | | | - Nádia Carolina Teixeira Marques
- - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Odontopediatria, Ortodontia e Saúde Coletiva, Bauru, SP, Brasil
| | | | - Carlos Ferreira Santos
- - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil
| | - Thais Marchini Oliveira
- - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Odontopediatria, Ortodontia e Saúde Coletiva, Bauru, SP, Brasil
| | - Vivien Thiemy Sakai
- - Universidade Federal de Alfenas, Departamento de Clínica e Cirurgia, Alfenas, MG, Brasil
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35
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Moura-Netto C, Ferreira LS, Maranduba CM, Mello-Moura ACV, Marques MM. Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency. Braz Oral Res 2016; 30:S1806-83242016000100265. [PMID: 27253140 DOI: 10.1590/1807-3107bor-2016.vol30.0080] [Citation(s) in RCA: 24] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/07/2015] [Accepted: 03/16/2016] [Indexed: 01/28/2023] Open
Abstract
Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cells, which could improve tissue engineering. SHEDs (eighth passage) were seeded into 96-well culture plates (103 cells/well) and were grown in culture medium supplemented with 15% defined fetal bovine serum (FBS) for 12 h. After determining the appropriate nutrition deficiency status (5% FBS), the cells were assigned into four groups: 1) G1 - 15% FBS (positive control); 2) G2 - 5% FBS (negative control); 3) G3 - 5% FBS+LPT 3 J/cm2; and 4) G4 - 5% FBS+LPT 5 J/cm2. For the LPT groups, two laser irradiations at 6 h intervals were performed using a continuous wave InGaAlP diode laser (660 nm, with a spot size of 0.028 cm2, 10 mW) in punctual and contact mode. Cell viability was assessed via an MTT reduction assay immediately after the second laser irradiation (0 h) and 24, 48, and 72 h later. We found that G3 and G4 presented a significantly higher cell growth rate when compared with G2 (p < 0.01). Moreover, G4 exhibited a similar cell growth rate as G1 throughout the entire experiment (p > 0.05). These findings indicate that LPT with 5 J/cm2 can enhance the growth of SHEDs during situations of nutritional deficiency. Therefore, LPT could be a valuable adjunct treatment in tissue engineering when using stem cells derived from the dental pulp of primary teeth.
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Affiliation(s)
- Cacio Moura-Netto
- Universidade Cruzeiro do Sul, Universidade Cruzeiro do Sul, School of Dentistry, São Paulo SP , Brazil, Universidade Cruzeiro do Sul, School of Dentistry, São Paulo, SP, Brazil
| | - Leila Soares Ferreira
- Universidade Ibirapuera, Universidade Ibirapuera, School of Dentistry, São Paulo SP , Brazil, Universidade Ibirapuera, School of Dentistry, São Paulo, SP, Brazil
| | - Carlos Magno Maranduba
- Universidade Federal de Juiz de Fora, Universidade Federal de Juiz de Fora, Institute of Biological Sciences, Department of Biology, Juiz de Fora MG , Brazil, Universidade Federal de Juiz de Fora, Institute of Biological Sciences, Department of Biology, Juiz de Fora, MG, Brazil
| | - Anna Carolina Volpi Mello-Moura
- Universidade Ibirapuera, Universidade Ibirapuera, School of Dentistry, São Paulo SP , Brazil, Universidade Ibirapuera, School of Dentistry, São Paulo, SP, Brazil
| | - Marcia Martins Marques
- Universidade de São Paulo, Universidade de São Paulo, School of Dentistry, Department of Restorative Dentistry, São Paulo SP , Brazil, Universidade de São Paulo (USP), School of Dentistry, Department of Restorative Dentistry, São Paulo, SP, Brazil
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36
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da Cruz Andrade PV, Euzebio Alves VT, de Carvalho VF, De Franco Rodrigues M, Pannuti CM, Holzhausen M, De Micheli G, Conde MC. Photodynamic therapy decrease immune-inflammatory mediators levels during periodontal maintenance. Lasers Med Sci 2016; 32:9-17. [PMID: 27704296 DOI: 10.1007/s10103-016-2076-7] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/23/2016] [Accepted: 09/05/2016] [Indexed: 12/14/2022]
Abstract
Antimicrobial photodynamic therapy (aPDT) was introduced as a promising adjuvant therapy on the periodontal treatment. The aim of this study was to evaluate the effect of aPDT on inflammatory mediator levels in residual periodontal pockets of patients with severe chronic periodontitis under periodontal maintenance, during 12 months follow-up. A randomized controlled trial study was conducted in 28 patients with severe chronic periodontitis. After non-surgical periodontal treatment, patients with at least four teeth with residual pocket probing depth (PPD) ≥4 mm were randomly assigned to either aPDT or control group. The aPDT (low power laser: 660 nm, 40 mW, 90 J/cm2, methylene blue 0.01 %) was performed at baseline and 3, 6, and 9 months. Clinical parameters were collected before and 3 and 12 months after the intervention, and gingival crevicular fluid was collected in the same times, including 1 week after the intervention. Immunological evaluation was carried out using the Luminex assay which quantified the expression of ten cytokines: interleukin (IL)-1α, IL-1β, IL-8, IL-1ra, fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-4, and IL-10. All clinical variables showed significant improvement for both groups, but there was no statistical difference between groups with no clinical benefits. IL-1α, IL-1β, IL-8, and VEGF showed significant differences (p < 0.05) between groups, whereas IL-1ra mediators, IFN-γ, and IL-10 demonstrated a statistical difference (p < 0.01) over time in the same group. At any time, FGF, IL-4, and TNF-α showed no statistical difference between groups (p > 0.05). aPDT therapy can improve the benefits on inflammation control during the periodontal maintenance.
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Affiliation(s)
- Priscila Vivas da Cruz Andrade
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Vanessa Tubero Euzebio Alves
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Verônica Franco de Carvalho
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Michelle De Franco Rodrigues
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Claudio Mendes Pannuti
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Marinella Holzhausen
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Giorgio De Micheli
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil
| | - Marina Clemente Conde
- Division of Periodontics, Department of Stomatology, School of Dentistry, University of Sao Paulo, Avenida Professor Lineu Prestes, 2227, Cidade Universitaria, Sao Paulo, SP, CEP: 05508-000, Brazil.
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37
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Pansani TN, Basso FG, Turrioni APS, Soares DG, Hebling J, de Souza Costa CA. Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals. Lasers Med Sci 2016; 32:45-52. [PMID: 27677475 DOI: 10.1007/s10103-016-2081-x] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/09/2016] [Accepted: 09/19/2016] [Indexed: 11/28/2022]
Abstract
This study evaluated the effects of low-level laser therapy (LLLT) and epidermal growth factor (EGF) on fibroblasts obtained from young and elderly individuals. Gingival fibroblasts from young (Y) and elderly (E) individuals were seeded in wells of 24-well plates with Dulbecco's modified Eagle's medium (DMEM) containing 10 % of fetal bovine serum (FBS). After 24 h, the cells were irradiated (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J/cm2) or exposed to EGF (100 μM). After 72 h, cells were evaluated for viability, migration, collagen and vascular endothelial growth factor (VEGF) synthesis, and gene expression of growth factors. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 5 %). Y and E fibroblasts irradiated with laser or exposed to EGF showed increased viability and collagen synthesis. Enhanced cell migration was observed for Y fibroblasts after both treatments, whereas only the LLLT stimulated migration of E cells. VEGF synthesis was higher for Y and E cells exposed to EGF, while this synthesis was reduced when E fibroblasts were irradiated. Increased gene expression of VEGF was observed only for Y and E fibroblasts treated with LLLT. Regardless of a patient's age, the LLLT and EGF applications can biostimulate gingival fibroblast functions involved in tissue repair.
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Affiliation(s)
- Taisa Nogueira Pansani
- Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil
| | - Fernanda Gonçalves Basso
- Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil
| | - Ana Paula Silveira Turrioni
- Department of Pediatric Dentistry, School of Dentistry, University Federal Uberlândia (UFU), Uberlândia, Brazil
| | - Diana Gabriela Soares
- Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil
| | - Josimeri Hebling
- Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil
| | - Carlos Alberto de Souza Costa
- Department of Physiology and Pathology, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Humaita, 1680. Centro, 14801903, Araraquara, SP, Brazil.
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38
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The effect of low-level laser therapy on non-surgical periodontal treatment: a randomized controlled, single-blind, split-mouth clinical trial. Lasers Med Sci 2016; 31:1767-1773. [DOI: 10.1007/s10103-016-2047-z] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/24/2016] [Accepted: 08/05/2016] [Indexed: 12/28/2022]
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39
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Ren C, McGrath C, Jin L, Zhang C, Yang Y. Effect of diode low-level lasers on fibroblasts derived from human periodontal tissue: a systematic review of in vitro studies. Lasers Med Sci 2016; 31:1493-510. [DOI: 10.1007/s10103-016-2026-4] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/21/2015] [Accepted: 07/05/2016] [Indexed: 10/21/2022]
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40
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Szymczyszyn A, Doroszko A, Szahidewicz-Krupska E, Rola P, Gutherc R, Jasiczek J, Mazur G, Derkacz A. Effect of the transdermal low-level laser therapy on endothelial function. Lasers Med Sci 2016; 31:1301-7. [PMID: 27299570 PMCID: PMC4999456 DOI: 10.1007/s10103-016-1971-2] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/01/2016] [Accepted: 05/24/2016] [Indexed: 12/16/2022]
Abstract
The effect of low-level laser therapy (LLLT) on the cardiovascular system is not fully established. Since the endothelium is an important endocrine element, establishing the mechanisms of LLLT action is an important issue.The aim of the study was to evaluate the effect of transdermal LLLT on endothelial function.In this study, healthy volunteers (n = 40, age = 20–40 years) were enrolled. N = 30 (14 female, 16 male, mean age 30 ± 5 years) constituted the laser-irradiated group (LG). The remaining 10 subjects (6 women, 4 men, mean age 28 ± 5 years) constituted the control group (CG). Participants were subjected to LLLT once a day for three consecutive days. Blood for biochemical assessments was drawn before the first irradiation and 24 h after the last session. In the LG, transdermal illumination of radial artery was conducted (a semiconductor laser λ = 808 nm, irradiation 50 mW, energy density 1.6 W/cm2 and a dose 20 J/day, a total dose of 60 J). Biochemical parameters (reflecting angiogenesis: vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), angiostatin; antioxidative status: glutathione (GSH) and the nitric oxide metabolic pathway: symmetric dimethylarginine (SDMA), asymmetric dimethylarginine (ADMA) and l-arginine) were assessed. In the LG, a significant increase in GSH levels and considerable decrease in angiostatin concentration following the LLLT were observed. No significant differences in levels of the VEGF, FGF, SDMA, ADMA were observed.LLLT modifies vascular endothelial function by increasing its antioxidant and angiogenic potential. We found no significant differences in levels of the nitric oxide pathway metabolites within 24 h following the LLLT irradiation.
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Affiliation(s)
- Alicja Szymczyszyn
- Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.,Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Adrian Doroszko
- Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.,Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Ewa Szahidewicz-Krupska
- Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.,Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Piotr Rola
- Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Radosław Gutherc
- Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Jakub Jasiczek
- Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland
| | - Grzegorz Mazur
- Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland
| | - Arkadiusz Derkacz
- Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland. .,Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
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O'Connor M, Patil R, Yu J, Hickey R, Premanand K, Kajdacsy-Balla A, Benedetti E, Bartholomew A. Mesenchymal Stem Cells Synergize with 635, 532, and 405 nm Laser Wavelengths in Renal Fibrosis: A Pilot Study. Photomed Laser Surg 2016; 34:556-563. [PMID: 27244220 DOI: 10.1089/pho.2015.4025] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/06/2023] Open
Abstract
OBJECTIVE To address whether a single treatment of one of three visible light wavelengths, 635, 532, and 405 nm (constant wave, energy density 2.9 J/m2), could affect the hallmarks of established renal fibrosis and whether these wavelengths could facilitate mesenchymal stem cell (MSC) beneficence. BACKGROUND DATA Chronic kidney disease is a global health problem with only 20% receiving care worldwide. Kidneys with compromised function have ongoing inflammation, including increased oxidative stress and apoptosis, peritubular capillary loss, tubular atrophy, and tubulointerstitial fibrosis. Promising studies have highlighted the significant potential of MSC-based strategies to mitigate fibrosis; however, reversal of established fibrosis has been problematic, suggesting that methods to potentiate MSC effects require further development. Laser treatments at visible wavelengths have been reported to enhance mitochondrial potential and available cellular ATP, facilitate proliferation, and inhibit apoptosis. We hypothesized that laser-delivered energy might provide wavelength-specific effects in the fibrotic kidney and enhance MSC responses. MATERIALS AND METHODS Renal fibrosis, established in C57BL6 mice following 21 days of unilateral ureter obstruction (UUO), was treated with one of three wavelengths alone or with autologous MSC. Mitochondrial activity, cell proliferation, apoptosis, and cytokines were measured 24 h later. RESULTS Wavelengths 405, 532, and 635 nm all significantly synergized with MSC to enhance mitochondrial activity and reduce apoptosis. Proliferative activity was observed in the renal cortices following combined treatment with the 532 nm laser and MSC; endothelial proliferation increased in response to the 635 nm laser alone and to the combined effects of MSC and the 405 nm wavelength. Reductions of transforming growth factor-β were observed with 532 nm alone and when combined with MSC. CONCLUSIONS Specific wavelengths of laser energy appear to induce different responses in renal fibrotic tissue. These findings support further study in the development of a customized laser therapy program of combined wavelengths to optimize MSC effects in the treatment of renal fibrosis.
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Affiliation(s)
- Megan O'Connor
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Rachana Patil
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Jiangzhou Yu
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Richard Hickey
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Kavitha Premanand
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Andre Kajdacsy-Balla
- 2 Department of Pathology, University of Illinois at Chicago , Chicago, Illinois
| | - Enrico Benedetti
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois.,3 Department of Transplant Surgery, University of Illinois at Chicago , Chicago, Illinois
| | - Amelia Bartholomew
- 1 Department of Surgery, University of Illinois at Chicago , Chicago, Illinois
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de Freitas LF, Hamblin MR. Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS : A PUBLICATION OF THE IEEE LASERS AND ELECTRO-OPTICS SOCIETY 2016; 22:7000417. [PMID: 28070154 PMCID: PMC5215870 DOI: 10.1109/jstqe.2016.2561201] [Citation(s) in RCA: 834] [Impact Index Per Article: 92.7] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/05/2023]
Abstract
Photobiomodulation (PBM) also known as low-level laser (or light) therapy (LLLT), has been known for almost 50 years but still has not gained widespread acceptance, largely due to uncertainty about the molecular, cellular, and tissular mechanisms of action. However, in recent years, much knowledge has been gained in this area, which will be summarized in this review. One of the most important chromophores is cytochrome c oxidase (unit IV in the mitochondrial respiratory chain), which contains both heme and copper centers and absorbs light into the near-infra-red region. The leading hypothesis is that the photons dissociate inhibitory nitric oxide from the enzyme, leading to an increase in electron transport, mitochondrial membrane potential and ATP production. Another hypothesis concerns light-sensitive ion channels that can be activated allowing calcium to enter the cell. After the initial photon absorption events, numerous signaling pathways are activated via reactive oxygen species, cyclic AMP, NO and Ca2+, leading to activation of transcription factors. These transcription factors can lead to increased expression of genes related to protein synthesis, cell migration and proliferation, anti-inflammatory signaling, anti-apoptotic proteins, antioxidant enzymes. Stem cells and progenitor cells appear to be particularly susceptible to LLLT.
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Affiliation(s)
- Lucas Freitas de Freitas
- Programa de Pós-Graduação
Interunidades Bioengenharia, University of São Paulo, São Carlos -
SP, Brazil
- Wellman Center for Photomedicine, Harvard Medical School,
Boston, MA 02114, USA
| | - Michael R Hamblin
- Wellman Center for Photomedicine, Harvard Medical School,
Boston, MA 02114, USA
- Department of Dermatology, Harvard Medical School, Boston,
MA 02115, USA
- Harvard-MIT Division of Health Sciences and Technology,
Cambridge, MA 02139, USA
- Correspondence: Michael R Hamblin,
; Tel 1-617-726-6182
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Keskiner I, Lutfioğlu M, Aydogdu A, Saygun NI, Serdar MA. Effect of Photobiomodulation on Transforming Growth Factor-β1, Platelet-Derived Growth Factor-BB, and Interleukin-8 Release in Palatal Wounds After Free Gingival Graft Harvesting: A Randomized Clinical Study. Photomed Laser Surg 2016; 34:263-71. [PMID: 27088277 PMCID: PMC4892223 DOI: 10.1089/pho.2016.4094] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022] Open
Abstract
Objective: This study evaluated the impact of photobiomodulation (PBM) on the healing of the donor palatal area following free gingival graft (FGG) harvesting by examining changes in transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF)-BB, and interleukin (IL)-8 levels in palatal wound fluid (PWF). Material and methods: Thirty patients were selected and randomly assigned to receive PBM (laser group) or PBM sham (sham group) in the palatine area after FGG harvesting. A neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (1064 nm) was applied to the test sites immediately after surgery and every 24 h thereafter for 4 days. PWF was collected on Days 7 and 12, and PWF TGF-β1, PDGF-BB, and IL-8 levels were analyzed by enzyme-linked immunosorbent assays (ELISA). Results: PWF TGF-β1, PDGF-BB, and IL-8 levels were significantly lower on Day 12 than on Day 7 for both groups. PWF TGF-β1, PDGF-BB, and IL-8 levels of the laser group were significantly higher than those of sham group on Day 7 (p < 0.05). PWF TGF-β1 levels were also significantly higher in laser group than in the sham group on Day 12; however, differences in PDGF-BB and IL-8 levels between groups on Day 12 were statistically nonsignificant. Conclusions: Observed increases in PWF TGF-β1, PDGF-BB, and IL-8 levels suggest that PBM may accelerate wound healing by stimulating production of selected mediators.
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Affiliation(s)
- Ilker Keskiner
- Department of Periodontology, Ondokuz Mayis University Faculty of Dentistry, Samsun, Turkey
| | - Muge Lutfioğlu
- Department of Periodontology, Ondokuz Mayis University Faculty of Dentistry, Samsun, Turkey
| | - Ahmet Aydogdu
- Department of Periodontology, Baskent University Faculty of Dentistry, Istanbul Application and Research Center, Istanbul, Turkey
| | - N. Isil Saygun
- Department of Periodontology, Gülhane Military Medical Academy, Ankara, Turkey
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Basso FG, Turrioni APS, Almeida LF, Soares DG, Oliveira CF, Hebling J, de Souza Costa CA. Nutritional deprivation and LPS exposure as feasible methods for induction of cellular - A methodology to validate for vitro photobiomodulation studies. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2016; 159:205-10. [PMID: 27085052 DOI: 10.1016/j.jphotobiol.2016.04.001] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/11/2015] [Accepted: 04/03/2016] [Indexed: 12/19/2022]
Abstract
Previous studies have demonstrated that high biostimulation takes place when cells under stress are subjected to phototherapy by laser or light-emitting-diode (LED) devices. Several studies selected nutritional deprivation by reducing the concentration of fetal bovine serum (FBS) in the culture medium or the exposure of cultured cells to lipopolysaccharide (LPS) as an in vitro cellular stress condition. However, there are no data certifying that these stimuli cause stressful conditions for cultured cells. This investigation assessed the induction of cellular stress by decreasing the concentration of FBS or adding LPS to culture medium. Odontoblast-like cells (MDPC-23) were cultured in complete culture medium (DMEM) containing 10% FBS. After a 12-hour incubation period, the DMEM was replaced by fresh medium containing 10% FBS (control), low concentrations of FBS (0, 0.2, 0.5, 2, or 5%) or LPS from Escherichia coli (10μg/ml). After an additional 12-hour incubation, cell viability, total cell-counting, total protein production, and gene expression of heat shock protein 70 (HSP70) were assessed. Data were statistically analyzed by ANOVA complemented by the Tukey test, with 5% considered significant. Cell viability was negatively affected only for 0% FBS, while reduced viable cell numbers and total protein production were detected for FBS concentrations lower than 2%. Higher HSP70 gene expression was also observed for FBS concentrations lower than 2% and for cells exposed to LPS. The nutritional deprivation model with culture medium lower than 2% of FBS can be safely used to induce cellular stress for in vitro photobiomodulation studies.
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Affiliation(s)
- F G Basso
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil
| | - A P S Turrioni
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil
| | - L F Almeida
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil
| | - D G Soares
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil
| | - C F Oliveira
- Universidade de Ribeirão Preto, UNAERP - Ribeirão Preto, SP, Brazil
| | - J Hebling
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil
| | - C A de Souza Costa
- Araraquara School of Dentistry - Univ. Estadual Paulista, Araraquara, SP, Brazil.
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Micro-computed tomography and histomorphometric analysis of human alveolar bone repair induced by laser phototherapy: a pilot study. Int J Oral Maxillofac Surg 2015; 44:1521-8. [DOI: 10.1016/j.ijom.2015.08.989] [Citation(s) in RCA: 29] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/28/2015] [Revised: 06/30/2015] [Accepted: 08/24/2015] [Indexed: 12/14/2022]
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Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts. Int J Dent 2015; 2015:258941. [PMID: 26504463 PMCID: PMC4609450 DOI: 10.1155/2015/258941] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/13/2015] [Revised: 09/05/2015] [Accepted: 09/09/2015] [Indexed: 11/26/2022] Open
Abstract
Aim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors' secretion (EGF, bFGF, and VEGF). Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the following energy densities: 2.6 J/cm2, 5.3 J/cm2, 7.9 J/cm2, and 15.8 J/cm2. Controls were not irradiated. Cultures were examined for cell proliferation and growth factors' secretion after 24, 48, and 72 hours. All experimental procedures were performed in duplicate. Data were analyzed by Student's t-test (p < 0.05). Results. All laser-irradiation doses applied promoted a higher cell proliferation at 48 hours in a dose-response relationship compared to controls. This difference reached statistical significance for the cultures receiving 15.8 J/cm2 (p = 0.03). Regarding EGF, all laser irradiation doses applied promoted a higher secretion at 48 hours in a reverse dose-response pattern compared to controls. This difference reached statistical significance for the cultures receiving 2.6 J/cm2 (p = 0.04). EGF levels at the other time points, bFGF, and VEGF showed a random variation between the groups. Conclusion. Within the limits of this study, LLLT (Nd:YAG) may induce gingival fibroblasts' proliferation and upregulate the secretion of EGF. Further studies are needed to confirm these results.
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Low-level laser therapy in the treatment of recurrent aphthous ulcers: a systematic review. ScientificWorldJournal 2015; 2015:150412. [PMID: 25879049 PMCID: PMC4386290 DOI: 10.1155/2015/150412] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2014] [Accepted: 10/31/2014] [Indexed: 11/23/2022] Open
Abstract
Recurrent aphthous ulcers (RAUs) are the most common lesion found in the oral cavity. There is no definitive cure for RAUs and current treatments are aimed at minimizing symptoms. Since low-level laser therapy (LLLT) modulates inflammatory responses, and promotes pain reduction and cellular biostimulation, LLLT can be suggested as an alternative treatment for RAUs. The literature concerning the potential of LLLT in the treatment of RAUs was evaluated. A systematic literature review identified 22 publications, of which only 2 studies were adopted. The eligibility criteria consisted of randomized controlled trials (RCTs). Both RCTs achieved significant results concerning LLLT and pain-level reductions and reduced healing times. Despite the variance in irradiation conditions applied in both studies, very similar wavelengths were adopted. There is accordingly strong evidence that wavelength plays an important role in RAU treatment. Taking into account the different parameters applied by selected RCTs, it is not possible to suggest that a specific protocol should be used. However, in light of the significant results found in both studies, LLLT can be suggested as an alternative for RAU treatment. Additional RCTs should be performed in order to reach a clinical protocol and better understand the application of LLLT in RAU treatment.
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Passanezi E, Damante CA, de Rezende MLR, Greghi SLA. Lasers in periodontal therapy. Periodontol 2000 2014; 67:268-91. [DOI: 10.1111/prd.12067] [Citation(s) in RCA: 38] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 02/02/2014] [Indexed: 12/12/2022]
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Pacheco PS, de Oliveira FA, Oliveira RC, Sant'ana ACP, de Rezende MLR, Greghi SLA, Damante CA. Laser phototherapy at high energy densities do not stimulate pre-osteoblast growth and differentiation. Photomed Laser Surg 2014; 31:225-9. [PMID: 23639292 DOI: 10.1089/pho.2012.3434] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022] Open
Abstract
OBJECTIVE The aim of this study is to evaluate the effects of red and infrared lasers at high energy densities on pre-osteoblast MC3T3 proliferation and differentiation. BACKGROUND DATA The acceleration of bone regeneration by low intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. MATERIALS AND METHODS Cells were irradiated with red (660 nm) and infrared (780 nm) lasers (90 and 150 J/cm2, 40 mW). The control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, 96 h) and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72, 96 h; 7, 14 days). RESULTS None of the irradiation groups had an enhancement in cell growth (p<0.05). The production of ALP was not influenced by irradiation at any period of time (p>0.05). CONCLUSIONS The low intensity laser stimulated neither cell growth nor the production of alkaline phosphatase.
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50
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Pansani TN, Basso FG, Turirioni APS, Kurachi C, Hebling J, de Souza Costa CA. Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid. Int J Oral Maxillofac Surg 2014; 43:1030-4. [PMID: 24656494 DOI: 10.1016/j.ijom.2014.02.011] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/11/2013] [Revised: 02/20/2014] [Accepted: 02/24/2014] [Indexed: 11/30/2022]
Abstract
Low-level laser therapy (LLLT) has been indicated as an adjuvant therapy for bisphosphonate-induced osteonecrosis. However, the effects of LLLT on bisphosphonate-treated cells are not yet clear. This study evaluated the effects of LLLT on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid (ZA). Cells were exposed to ZA at 5μM for 48h. Irradiation was performed using a laser diode prototype (LaserTABLE, InGaAsP; 780nm±3nm, 25mW) at 0.5 or 3J/cm(2), three times every 24h. Cell proliferation and apoptosis were evaluated by fluorescence microscopy. Data were analyzed by Mann-Whitney test at the 5% level of significance. ZA decreased cell proliferation to 47.62% (interquartile range (IQR) 23.80-57.14%; P=0.007) and increased apoptosis of gingival fibroblasts to 27.7% (IQR 20.9-33.4%; P=0.0001). LLLT increased cell proliferation compared with non-irradiated cells, at 0.5J/cm(2) (57.14%, IQR 57.14-71.43%; P=0.003) and at 3J/cm(2) (76.19%, IQR 61.90-76.19%; P=0.0001), but did not increase cell proliferation in ZA-treated cells. Irradiated fibroblasts presented lower apoptosis rates than the ZA-treated cells, but apoptosis was no different in ZA-treated cells compared to those that were ZA-treated and also irradiated.
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Affiliation(s)
- T Nogueira Pansani
- Araraquara School of Dentistry, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil
| | - F Gonçalves Basso
- Araraquara School of Dentistry, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil.
| | - A P Silveira Turirioni
- Araraquara School of Dentistry, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil
| | - C Kurachi
- Physics Institute, USP - Universidade de São Paulo, São Carlos, SP, Brazil
| | - J Hebling
- Araraquara School of Dentistry, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil
| | - C A de Souza Costa
- Araraquara School of Dentistry, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil
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