The surface Plasmon resonance (SPR) sensing technique combines rapid response, high sensitivity, non-destructive operation, high specificity, real-time on-site detection, and continuous monitoring advantages. These distinctive features enable its broad application in detecting disease biomarkers, viruses, foodborne contaminants, and hazardous gases. To enhance SPR sensing signals, metal-organic frameworks (MOFs), which emerge as a category of highly porous-organic frameworks, have demonstrated superior performance as SPR sensitive layers due to their features of large specific surface areas, high porosity, regular skeletons, tunable chemical components and functionality, and promising optoelectronic performances. Varieties of MOFs (such as UiO-66, porphyrin-based MOFs, and zeolitic imidazolate frameworks) and MOFs-based composites with improved plasmon-exciton coupling and charge carrier mobility exhibit amplified SPR responses through the integration of diverse effects. This review systematically summarizes the construction principle of MOFs-based SPR sensors and discusses their advancements for the inspection of biomarkers, drug residuals, viruses, and volatile organic gases. Finally, we also analyze the current challenge and provide the perspective about this field, which can inspire readers to a certain extent.

Breast cancer is the second-most common cause of cancer-related death among women worldwide, with a gradual annual increase of 0.5 % in its occurrence rate in recent years. This complex ailment exhibits considerable diversity, with a mortality rate of 2.5 %. One promising area of research for its treatment is the development of MOFs, which are intricate three-dimensional (3D) structures constructed from metal ions or clusters joined with organic ligands through coordinate bonds. MOFs have emerged as versatile platform overcoming the limitations of conventional chemotherapeutics including poor drug solubility, non-specific targeting, and multidrug resistance. These applications are attributed to their adjustable porosity, chemical makeup, dimensions, straightforward surface customization capabilities, biocompatibility, nontoxicity etc. These properties position MOFs as excellent candidates for diverse regimes of cancer therapeutics including innovative approaches such as phototherapy, chemotherapy, immunotherapy, gene therapy, sonodynamic therapy, and various combination therapies. The article emphasizes the functionalization and applications of MOFs, with a primary focus on their therapeutic capabilities, synergistic approaches, and theranostic strategies that integrate diagnostic and therapeutic functions. Strategies to improve MOF biocompatibility and stability, such as surface modifications and biocompatible coatings are also discussed. Insights on various challenges and future prospects are provided to address current limitations and inspire further research, paving the way for clinical translation of MOF-based breast cancer therapies.

l-Cysteine (L-Cys), one kind of aminothiol, ubiquitously exists in proteins and plays important roles in the food industry and biological processes. Herein, an electrochemiluminescence (ECL) sensor was constructed for the sensitive detection of L-Cys. As an ECL emitter, europium-porphyrin coordination polymer (Eu-PCP) was synthesized and subsequently modified with copper benzene-1,4-dicarboxylate (CuBDC) as a coreaction accelerator, which achieved high self-enhanced ECL responses. CuBDC on Eu-PCP was decomposed by the introduced L-Cys due to the coordination between Cu2+ and L-Cys, resulting in the decrease of ECL signal. Besides, L-Cys could consume the co-reactant of K2S2O8 to reduce the production of free radical SO4•-, further decrease ECL signal. Thus, this L-Cys coordination-driven decomposition of coreaction accelerator and consumption of co-reactant strategy was employed to doubly suppress the ECL signal for detecting L-Cys without additional recognition and amplification elements. The constructed ECL sensor exhibited a linear range of 1.0 × 10-10-5.0 × 10-5 mol/L with a limit of detection of 2.0 × 10-11 mol/L. This work has opened up new prospects for developing novel ECL sensing strategies in food safety monitoring and disease diagnosis.

Monitoring mercury (Hg) is critical for environmental and public health. Metal-organic framework (MOF)-based sensors demonstrate the advantage of high sensitivity and rapid response. We summarize the advances of MOF sensors for Hg2+ detection from the perspective of MOF type and role in the sensors. First, we introduce three MOFs used in Hg sensors-UIO, ZIF, and MIL-that have demonstrated superior performance. Then, we discuss the specifics of MOF-based sensors for Hg2+ detection in terms of the recognition and signal elements. Currently, the recognition elements include T-rich aptamers, noble metal nanoparticles, central metal ions, and organic functional groups inherent to MOFs. Sensors with fluorescence and colorimetric signals are the two main types of optical MOF sensors used for Hg detection. Electrochemical sensors have also been fabricated, but these are less frequently reported, potentially due to the limited conductivity and cycling stability of MOFs. Notably, dual-signal sensors mitigate background signals interference and enhance the accuracy of Hg2+ detection. Furthermore, to facilitate portability and user-friendliness, portable devices such as microfluidics, paper-based devices, and smartphones have been developed for Hg2+ detection, showcasing potential applications. We also address the challenges related to MOF-based sensors for Hg2+ and future outlook.

Skin cancer, a common malignancy worldwide, has increased incidence and mortality. Thus, it is a public health issue and a significant illness burden, which increases treatment costs. Chemotherapy and surgery are used to treat skin cancer. However, conventional skin cancer treatments have several limitations, demanding the development of innovative, safe, and effective methods. To overcome these limitations of conventional topical dosage forms, many nanocarriers have been developed and tested for the targeted delivery of anticancer drugs.

The main objective of the present review was to discuss the utility of various vesicular nanocarrier systems to deliver anticancer drugs following topical administration to treat skin cancer.

For this review article, we scoured the scholarly literature using Science Direct, Google Scholar, and PubMed.

The vesicular drug delivery system has been intensively explored and developed as an alternative to conventional skin cancer drug delivery systems, especially for melanoma. They improve the penetration of anticancer drugs via the skin, reaching the cancer area with enough and killing cancer cells. Vesicles minimize skin irritation and drug degradation. This improves therapy efficacy and reduces systemic toxicity.

Utilizing the vesicular drug delivery system shows promise in treating skin cancer. Therefore, further research and inquiries are necessary to explore the therapeutic potential of these substances in treating skin cancer, intending to develop a personalized, efficient, and secure therapy approach for patients with this condition.

The oxidative-reduction electrochemiluminescence (ECL) potential of a luminophore is one of the most significant parameters during light generation processes when considering the growing demand for anti-interference analysis techniques, electrode compatibility and the reduction of damage to biological molecules due to excessive excitation potential. Nanoparticle luminophores, including quantum dots (QDs) and metal nanoclusters (NCs), possess tremendous potential for forming various ECL sensors due to their adjustable surface states. However, few reviews focused on nanoparticle luminophore-based ECL systems for low-triggering-potential (LTP) oxidative-reduction ECL to avoid the possible interference and oxidative damage of biological molecules. This review summarizes the recent advances in the LTP oxidative-reduction ECL potential strategy with nanoparticle luminophores as ECL emitters, including matching efficient coreactants and nanoparticle luminophores, doping nanoparticle luminophores, constructing donor-acceptor systems, choosing suitable working electrodes, combining multiplex nanoparticle luminophores, and employing surface-engineering strategies. In the context of the different LTP ECL systems, potential-lowering strategies and bio-related applications are discussed in detail. Additionally, the future trends and challenges of low ECL-triggering-potential strategies are discussed.

Metal-organic frameworks (MOFs) are composed of metal nodes and organic linkers that can self-assemble into an infinite network. The high porosity and large surface area of MOFs facilitate the effective enrichment and mass transfer of analytes, which can enhance the signal response and improve the sensitivity of electrochemical sensors. Additionally, MOFs and their derivatives possess the properties of unsaturated metal sites and tunable structures, collectively demonstrating their potential for electrochemical sensing. This paper summarizes the preparation methods, structural properties, and applications of MOFs and their derivatives in electrochemical sensing, emphasizing sensors' selectivity and sensitivity from the perspectives of direct and indirect detection. Additionally, it also explores future directions and prospects for MOFs in electrochemical sensing, with the aim of overcoming current limitations through innovative approaches.

Ochratoxin A (OTA) is a group of mycotoxins that are widely distributed in food and feed and are closely associated with human health, so it is particularly important to detect OTA in cereal-based foods. Porphyrins and their derivatives have been widely investigated for their excellent electrochemical luminescence properties. Tetrakis 4-carboxyphenyl) porphyrin (TCPP) has limited applications because of its tendency to aggregate in water.

To enhance the luminescence efficiency of TCPP, the porphyrin can be immobilized as an organic ligand in a metal-organic framework. This allows the preparation of a novel zinc-porphyrin-based MOF (Zn-TCPP nanorods), which in turn provides highly efficient and stable cathodic ECL signals. Herein, an ultra-sensitive electrochemiluminescence immunoassay was proposed using Zn-TCPP nanorods as high-efficiency luminophores and Bi2S3@Au nanoflowers as electrode substrate materials for the detection of ochratoxin A in foodstuffs. Zn-TCPP has a strong and stable signal, and has been used as an immunosensor probe material. The Bi2S3@Au nanoflowers was used to decorate the glass carbon electrode and support for antibody immobilization due to its good electrical conductivity and large specific surface area. Under the optimized conditions, the constructed immunosensor could realize the sensitive detection of ochratoxin A in the detection range of 0.0004 ng mL-1 to 500 ng mL-1 with the detection limit as low as 0.13 pg mL-1. In addition, the sensing platform has been used for the detection of OTA in wheat flour and feed.

Hence, it is worth believing that this strategy can pave a bright research direction for the detection of ochratoxin A or other small molecule mycotoxins content in foods, as well as contributing to the further study of MOF in the field of ECL.

To enhance the precision and reliability of early disease detection, especially in malignancies, an exhaustive investigation of multi-target biomarkers is essential. In this study, an advanced integrated electrochemical biosensor array that demonstrates exceptional performance was constructed. This biosensor was developed through a controllable porous-size mechanism and in-situ modification of carbon nanotubes (CNTs) to quantify multiplex biomarkers-specifically, C-reaction protein (CRP), carbohydrate antigen 125 (CA125), and carcinoembryonic antigen (CEA)-in human serum plasma. The fabrication process involved creating a highly ordered three-dimensional inverse-opal structure with the CNTs (pCNTs) modifier through microdroplet-based microfluidics, confined spatial self-assembly of nanoparticles, and chemical wet-etching. This innovative approach allowed for direct in-situ modification of nanomaterial onto the surface of electrode array, eliminating secondary transfer and providing exceptional control over structure and stability. The outstanding electrochemical performance was achieved through the synergistic effect of the pCNTs nanomaterial, aptamer, and horseradish peroxidase-labeled (HRP-) antibody. Additionally, the integrated biosensor array platform comprised multiple individually addressable electrode units (n = 11), enabling simultaneous multi-parallel/target testing, thereby ensuring accuracy and high throughput. Crucially, this integrated biosensor array accurately quantified multiplex biomarkers in human serum, yielding results comparable to commercial methods. This integrated technology holds promise for point-of-care testing (POCT) in early disease diagnosis and biological analysis.

A metasurface as an artificial electromagnetic structure can concentrate optical energy into nanometric volumes to strongly enhance the light-matter interaction, which has been becoming a powerful platform for optical sensing, nonlinear effects, and quantum optics. Herein, we developed a novel hybrid plasmonic-dielectric metasurface consisting of Au nanorings (Au NRs) and TiO2 nanoparticles derived from MXene (TiO2 NPs@MXene). The hybrid metasurface simultaneously benefited from the high near-field enhancement effect of plasmonic materials and the low loss of dielectric materials. Furthermore, the optical modulation efficiency of the hybrid metasurface can be regulated by a magnetic mirror configuration. The magnetic mirror acted like a mirror, confining the electrons to a limited region and increasing the density of the surface plasmon. Moreover, the electrochemiluminescence (ECL) of the Cu2BDC metal-organic framework (Cu2BDC-MOF) served as a light source for the Au NRs/TiO2 NPs@MXene metasurface. Due to the exceptional light manipulation capability of the hybrid metasurface and the coordination of the magnetic mirror, the isotropic ECL signal can be dynamically amplified and converted into polarized emission. Finally, a metasurface-regulated ECL (MECL)-based biosensor with a dual-positive membrane protein recognition strategy was developed for the accurate identification of gastric cancer-derived extracellular vesicles. The novel MECL research opened up a new route in the realization of dynamically tunable metasurfaces for optical sensing and novel nanophotonic devices.

Abnormal cell growth and proliferation can lead to tumor formation and cancer, one of the most fatal diseases worldwide. Hydrogen peroxide (H2O2) has emerged as a cancer biomarker, with its concentration being crucial for distinguishing cancer cells from normal cells. Herein, a cost-effective and enzymeless electrochemical sensing system for the monitoring of intracellular H2O2 has been constructed. The sensor is fabricated using gold nanoparticles embedded bimetallic copper/nickel metal organic framework (Au-CNMOF) immobilized reduced graphene oxide (RGO) modified screen printed electrode (SPE). The synthesized materials were characterized and confirmed by XRD, FTIR, SEM with EDS, and electrochemical analysis. The fabricated sensor displayed a redox peak at a formal potential (E°) of -0.155 V, corresponding to CuII/I redox couple of CNMOF in 0.1 M phosphate buffer. Electrochemical investigations revealed that the proposed sensor has a large electrochemical active surface area (1.113 cm2) and a higher surface roughness (5.67). Additionally, the sensor demonstrated excellent electrocatalytic activity towards H2O2 at -0.3 V, over a wide linear detection range from 28.5 µM to 4.564 mM with a limit of detection of 4.2 µM (S/N=3). Furthermore, the proposed sensor exhibits excellent stability, repeatability, reproducibility, and good anti-interference activity. Ultimately, the sensor was validated through real-time analysis of H2O2 released from cancer cells, successfully quantifying the released H2O2. The developed sensor holds great promise for real-time H2O2 analysis, with potential applications in clinical diagnostics, biological research and environmental monitoring.

COVID-19 as an infectious disease with rapid transmission speed is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), so, early and accurate diagnostics of COVID-19 is quite challenging. In this work, the selective and sensitive self-enhanced ECL method to detect of SARS-CoV-2 protein was designed with magnetic N-doped carbon derived from dual-ligand metal-organic frameworks (MOF) (CoO@N-C) with the primary and tertiary amino groups as a novel coreactant that covalently combined with Ru(bpy)2(phen-NH2)2+ as electrochemiluminescence (ECL) emitter. Mixed-ligand strategy and selected nitrogen-containing ligands, 4,4',4''-((1,3,5-triazine-2,4,6-triyl) tris-(azanediyl)) tribenzoic acid (H3TATAB) with 2-aminoterephthalic acid (BDC-NH2) were used for synthesis of the proposed MOF. Also, magnetic CoO@N-C with high synergistically charge transfer kinetics and good stability can be used as an effective platform/coreactor on the ITO electrode which load more Ru-complex as signal producing compound and SARS-CoV-2 N protein antibody to increase the sensitivity of the immunosensor. Furthermore, (CoO@N-C) as coreactor improved the ECL signal of the Ru (II)-complex more than 2.1 folds compared to tripropylamine. In view of these competences, the novel "on-off" ECL biosensor performed with great stability and repeatability for detection of SARS-CoV-2 protein, which exhibited a broad linearity from 8 fg. mL-1 to 4 ng. mL-1 (6 order of magnitude) and an ultra-low limit of detection 1.6 fg. mL-1. Finally, this proposed method was successfully applied to detect of SARS-CoV-2 N protein in serum sample with satisfactory results, indicating the proposed immunosensor has the potential for quick analysis of SARS-CoV-2.

Exploring the construction of an interface with bright emission, fabulous stability, and good function to develop high-performance electrochemiluminescence (ECL) biosensors for tumor biomarkers is in high demand but faces a huge challenge. Herein, we report an oriented attachment and in situ self-assembling strategy for one-step fabrication of CdTe QD-encapsulated Hf polymer membrane onto an ITO surface (Hf-CP/CdTe QDs/APS/ITO). Hf-CP/CdTe QDs/APS/ITO is fascinating with excellent stability, high ECL emission, and specific adsorption toward ssDNA against dsDNA and mononucleotides (mNs). These interesting properties make it an ideal interface to rationally develop an immobilization-free ECL biosensor for cancer antigen 125 (CA125), used as a proof-of-concept analyte, based on target-aptamer recognition-promoted exonuclease III (Exo III)-assisted digestion. The recognition of ON by CA125 leads to the formation of CA125@ON, which hybridizes with Fc-ssDNA to switch Exo III-assisted digestion, decreasing the amount of Fc groups anchored onto the electrode's surface and blocking electron transfer. As compared to the case where CA125 was absent, significant ECL emission recovery is determined and relies on CA125 concentration. Thus, highly sensitive analysis of CA125 against other biomarkers was achieved with a limit of detection down to 2.57 pg/mL. We envision this work will provide a new path to develop ECL biosensors with excellent properties, which shows great potential for early and accurate diagnosis of cancer.

Metal-organic frameworks (MOFs), as porous materials, have great potential for exploring high-performance electrochemiluminescence (ECL) probes. However, the constrained applicability of MOFs in the realm of ECL biosensing is primarily attributed to their inadequate water stability, which consequently impairs the overall ECL efficiency. Herein, we developed a competitive ECL biosensor based on a novel tightest structural ruthenium-based organic framework emitter combining the proximity hybridization-induced catalytic hairpin assembly (CHA) strategy and the quenching effect between the Ru-MOF and ferrocene for detecting paraquat (PQ). Through a simple hydrothermal synthesis strategy, ruthenium and 2,2'-bipyrimidine (bpm) are head-to-head self-assembled to obtain a novel tightest structural Ru-MOF. Due to the metal-ligand charge-transfer (MLCT) effect between ruthenium and the bpm ligand and the connectivity between the internal chromophore units, the Ru-MOF exhibits strong ECL emissions. Meanwhile, the coordination-driven Ru-MOF utilizes strong metal-organic coordination bonds as building blocks, which effectively solves the problem of serious leakage of chromophores caused by water solubility. The sensitive analysis of PQ is realized in the range of 1 pg/mL to 1 ng/mL with a detection limit of 0.352 pg/mL. The tightest structural Ru-MOF driven by the coordination of ruthenium and bridging ligands (2,2'-bipyrimidine, bpm) provides new horizons for exploring high-performance MOF-based ECL probes for quantitative analysis of biomarkers.

Polycyclic aromatic hydrocarbons (PAHs) are excellent alternative luminophores for electrochemiluminescence (ECL) immunoassays. However, they are inevitably limited by the aggregation-caused quenching effect. In this study, aimed at eliminating the aggregation quenching of PAHs, luminescent metal-organic frameworks (MOFs) with 1,3,6,8-tetra(4-carboxybenzene)pyrene (H4TBAPy) as the ligand were exploited as a novel nano-emitter for the construction of ECL immunoassays. The luminophore exhibits efficient aggregation-induced emission enhancement, good acid-base resistance property and unusual ECL reactivity. In addition, the simultaneous use of potassium persulfate and hydrogen peroxide as dual co-reactants resulted in a synergistic enhancement of the cathodic ECL efficiency. The use of magnetic iron-nickel alloys as the multifunctional sensing platform can further enhance the ECL activity, and its enriched zero-valent iron as a co-reactant accelerator effectively drives ECL analytical performance. Profiting from the excellent characteristics, signal-on ECL immunoassays have been constructed. With carcinoembryonic antigen as the model analysis target, a detection limit of 0.63 pg/mL was obtained within the linear range of 1 pg/mL to 50 ng/mL, accompanied by excellent analytical performance. This report opens a new window for the rational design of efficient ECL illuminators, and the proposed ECL immunoassays may find promising applications in the detection of disease markers.

The reported organic electrochemiluminescence (ECL) luminophors for the detection of various markers often suffer from intermolecular π-π stacking-induced luminophore quenching. Herein, we demonstrate one-pot synthesis of a new aggregation-induced electrochemiluminescence (AIECL) emitter (i.e., TPE@SiO2/rGO composite) for sensitive measurement of microcystin-leucine arginine (MC-LR). The TPE@SiO2/rGO composite is constructed by embedding the silica-encapsuled 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TPE) in the reduced graphene oxide. In comparison with the monomer TPE, this composite exhibit high luminescence efficiency and strong ECL emission, because the AIECL phenomenon triggered by the spatial confinement effect in the SiO2 cage induces the restriction of the internal motion and vibration of molecules. Notably, this composite has distinct advantages of easy preparation, simple functionalization, and stable luminescence. Especially, the TPE@SiO2/rGO-based ECL-RET system exhibits a high quenching efficiency (ΦET) of 69.7%. When target MC-LR is present, it triggers DNA strand displacement reaction (SDR), inducing the quenching of the ECL signal of TPE@SiO2/rGO composite due to ECL resonance energy transfer between TPE@SiO2/rGO composite and methylene blue (MB). The proposed biosensor enables highly sensitive, low-cost, and robust measurement of MC-LR with a large dynamic range of 7 orders of magnitude and a detection limit of 3.78 fg/mL, and it displays excellent detection performance in complex biological matrices, holding potential applications in food safety and water monitoring.

The construction of assays is capable of accurately detecting cytokeratin-19 (CYFRA 21-1), which is critical for the rapid diagnosis of nonsmall cell lung cancer. In this work, a novel electrochemiluminescence (ECL) immunosensor based on the co-reaction promotion of luminol@Au@Ni-Co nanocages (NCs) as ECL probe by Ti3C2Tx MXene@TiO2-MoS2 hybrids as co-reaction accelerator was proposed to detect CYFRA 21-1. Ni-Co NCs, as a derivative of Prussian blue analogs, can be loaded with large quantities of Au NPs, luminol, and CYFRA 21-1 secondary antibodies due to their high specific surface area. To further improve the sensitivity of the developed ECL immunosensor, Ti3C2Tx MXene@TiO2-MoS2 hybrids were prepared by in situ growth of TiO2 nanosheets on highly conductive Ti3C2Tx MXene, and MoS2 was homogeneously grown on Ti3C2Tx MXene@TiO2 surfaces by the hydrothermal method. Ti3C2Tx MXene@TiO2-MoS2 hybrids possess excellent catalytic performance on the electro-redox of H2O2 generating more O2·- and obtaining optimal ECL intensity of the luminol/H2O2 system. Under the appropriate experimental conditions, the quantitative detection range of CYFRA 21-1 was from 0.1 pg mL-1 to 100 ng mL-1, and the limit of detection (LOD) was 0.046 pg mL-1. The present sensor has a lower LOD with a wider linear range, which provides a new analytical assay for the early diagnosis of small-cell-type lung cancer labels.

Carcinoembryonic antigens (CEAs) are prominent cancer biomarkers that enable the early detection of numerous cancers. For effective CEA screening, rapid, portable, efficient, and sensitive diagnosis approaches should be devised. Metal-organic frameworks (MOFs) are porous crystalline materials that have received major attention for application in high-efficiency signal probes owing to their advantages such as large specific surface area, superior chemical stability and tunability, high porosity, easy surface functional modification, and adjustable size and morphology. Immunoassay strategies using antigen-antibody specific interaction are one of the imperative means for rapid and accurate measurement of target molecules in biochemical fields. The emerging MOFs and their nanocomposites are synthesized with excellent features, providing promising potential for immunoassays. This article outlines the recent breakthroughs in the synthesis approaches of MOFs and overall functionalization mechanisms of MOFs with antigen/antibody and their uses in the CEA immunoassays, which operate according to electrochemical, electrochemiluminescent and colorimetric techniques. The prospects and limitations of the preparation and immunoassay applications of MOF-derived hybrid nanocomposites are also discussed at the end.

Improving the sensitivity in electrochemiluminescence (ECL) detection systems necessitates the integration of robust ECL luminophores and efficient signal transduction. In this study, we report a novel ECL nanoprobe (Zr-MOF) that exhibits strong and stable emission by incorporating aggregation-induced emission ligands into Zr-based metal-organic frameworks (MOFs). Meanwhile, we designed a high-performance signal modulator through the implementation of a well-designed controlled release system with a self-on/off function. ZnS quantum dots (QDs) encapsulated within the cavities of aminated mesoporous silica nanoparticles (NH2-SiO2) serve as the ECL quenchers, while adenosine triphosphate (ATP) aptamers adsorbed on the surface of NH2-SiO2 through electrostatic interaction act as "gatekeepers." Based on the target-triggered ECL resonance energy transfer between Zr-MOF and ZnS QDs, we establish a coreactant-free ECL aptasensor for the sensitive detection of ATP, achieving an impressive low detection limit of 0.033 nM. This study not only demonstrates the successful combination of ECL with controlled release strategies but also opens new avenues for developing highly efficient MOFs-based ECL systems.

Cancer antigen 125 (CA125) is a typical tumor marker of ovarian cancer. Here, a multi-amplified electrochemiluminescence (ECL) aptasensor was developed for efficient recognition of CA125 using tetrahedral DNA nanostructure (TDN) enhanced toehold-mediated strand displacement (TMSD) coupled with gold nanoparticles/Ru(bpy)32+/metal-organic framework (AuNPs/Ru/ZIF-MOF) signal probe. AuNPs and Ru(bpy)32+ modified ZIF-MOF acted as initial ECL signal and further used for the immobilization of TDN, the activated DNA templates on the surface of TDN were firstly hybridized with ferrocene labeled DNA probe (S6) and S5, in which, S6 acted as the energy acceptor of ECL signal from Ru(bpy)32+, making the sensor in a "signal-off" state. After the specific recognition of aptamer (AP) with CA125, DNA initiator (S7) was freed to induce the happen of TMSD by using S8 as the helper DNA, accompanying with the release of S6 from the electrode surface and the recovery of Ru(bpy)32+ ECL signal, making the sensor in a "signal-on" state. Then, S7 was recycled for the next TMSD, making the sensor highly sensitive with a detection limit of 6 × 10-3 pg/mL. Moreover, the proposed aptasensor achieved high performance for CA125 detection in human serum samples, illustrating the reliability of the sensor in clinical analysis.

The commercialized electrochemiluminescence (ECL) immunoassay is carried out by holding luminophore Ru(bpy)32+ at a given potential. Designing an electrochemiluminophore with a narrow triggering potential window is strongly anticipated to decrease the electrochemical cross-talk and improve the flux of the commercialized ECL immunoassay in a potential-resolved way. Herein, L-penicillamine-capped silver nanoclusters (LPA-AgNCs) are facilely synthesized and utilized as tags to perform the ECL immunoassay with a sole and narrow triggering potential window of 0.24 V by employing hydrazine (N2H4) as a coreactant. The maximum ECL emission of the LPA-AgNCs/N2H4 system is located ca. +1.27 V. Upon immobilizing LPA-AgNCs onto the electrode surface via forming a sandwich immunocomplex, the ECL of LPA-AgNCs/N2H4 can be utilized to sensitively and selectively determine human carcinoembryonic antigen from 0.5 to 1000 pg/mL with a low limit of detection of 0.1 pg/mL (S/N = 3). This work might open a way to screen electrochemiluminophores for the multiple ECL immunoassay in a potential-resolved way.

Carcinoembryonic antigen (CEA) is a significant glycosylated protein, and the unusual expression of CEA in human serum is used as a tumor marker in the clinical diagnosis of many cancers. Although scientists have reported many ways to detect CEA in recent years, such as electrochemistry, photoelectrochemistry, and fluorescence, their operation is complex and sensitivity is average. Therefore, finding a convenient method to accurately detect CEA is significance for the prevention of malignant tumors. With high sensitivity, quick reaction, and low background, electrochemiluminescence (ECL) has emerged as an essential method for the detection of tumor markers in blood.

In this work, a "signal on-off" ECL immunosensor for sensitive analysis of CEA ground on the ternary extinction effects of CuFe2O4@PDA-MB towards a self-enhanced Ru(dcbpy)32+ functionalized metal-organic layer [(Hf)MOL-Ru-PEI-Pd] was prepared. The high ECL efficiency of (Hf)MOL-Ru-PEI-Pd originated from the dual intramolecular self-catalysis, including intramolecular co-reaction between polyethylenimine (PEI) and Ru(dcbpy)32+. At the same time, loading Pd NPs onto (Hf)MOL-Ru-PEI could not only improve the electron transfer ability of (Hf)MOL-Ru-PEI, but also provide more active sites for the reaction of Ru(dcbpy)32+ and PEI. In the presence of CEA, CuFe2O4@PDA-MB-Ab2 efficiently quenches the excited states of (Hf)MOL-Ru-PEI-Pd by PDA, Cu2+, and methylene blue (MB) via energy and electron transfer, leading to an ECL signal decrease. Under optimal conditions, the proposed CEA sensing strategy showed satisfactory properties ranging from 0.1 pg mL-1 to 100 ng mL-1 with a detection limit of 20 fg mL-1.

The (Hf)MOL-Ru-PEI-Pd and CuFe2O4@PDA-MB were prepared in this work might open up innovative directions to synthesize luminescence-functionalized MOLs and effective quencher. Besides, the ECL quenching mechanism of Ru(dcbpy)32+ by MB was successfully explained by the inner filter effect (ECL-IFE). At last, the proposed immunosensor exhibits excellent repeatability, stability, and selectivity, and may provide an attractive way for CEA and other disease markers determination.

2D metal-organic frameworks-based (2D MOF-related) materials benefit from variable topological structures, plentiful open active sites, and high specific surface areas, demonstrating promising applications in gas storage, adsorption and separation, energy conversion, and other domains. In recent years, researchers have innovatively designed multiple strategies to avoid the adverse effects of conventional methods on the synthesis of high-quality 2D MOFs. This review focuses on the latest advances in creative synthesis techniques for 2D MOF-related materials from both the top-down and bottom-up perspectives. Subsequently, the strategies are categorized and summarized for synthesizing 2D MOF-related composites and their derivatives. Finally, the current challenges are highlighted faced by 2D MOF-related materials and some targeted recommendations are put forward to inspire researchers to investigate more effective synthesis methods.

The modern lifestyle has increased our utilization of pollutants such as heavy metals, aromatic compounds, and contaminants that are of rising concern, involving pharmaceutical and personal products and other materials that may have an important environmental impact. In particular, the ultimate results of the intense use of highly stable materials, such as heavy metals and chemical restudies, are that they turn into waste materials, which, when discharged, accumulate in environmental water bodies. In this context, the present review presents the application of metal-organic frameworks (MOFs) in electrochemiluminescent (ECL) sensing for water pollutant detection. MOF composites applied as innovative luminophore or luminophore carriers, materials for electrode modification, and the enhancement of co-reaction in ECL sensors have enabled the sensitive monitoring of some of the most common contaminants of emerging concern such as heavy metals, volatile organic compounds, pharmaceuticals, industrial chemicals, and cyanotoxins. Moreover, we provide future trends and prospects associated with ECL MOF composites for environmental sensing.

The non-specific adsorption behaviors of various interferents on the surface of a molecularly imprinted polymer (MIP) are adverse for the selectivity of an MIP-based sensor, which can be overcome via a differential strategy by using the differential signal between MIP- and non-imprinted polymer (NIP)-based sensors. However, the normal differential mode is not suitable for the MIP-based sensors with non-linear calibration curves. Herein, an improved differential strategy is reported for an MIP-based sensor with a semi-logarithmic calibration curve, demonstrated by an electrochemiluminescence (ECL) sensor for dopamine (DA). Glassy carbon electrode (GCE) was modified by the mixture of g-C3N4, TiO2 nanoparticles (NPs) and carbon nanotubes (CNTs). MIP membrane for DA was fabricated on the surface of g-C3N4/TiO2NPs/CNTs/GCE using chitosan for film-forming, obtained MIP@GCE. To enhance the anti-interference ability of the MIP-based DA sensor, the difference between exponential functions ECL intensities of MIP@GCE and NIP@GCE is used as the analytical signal in the improved differential strategy. The differential signal was increased linearly with increasing DA concentration ranging from 10 pM to 0.10 μM, with the detection limit of 5.6 pM. The interference level of Cu2+ on DA determination in the improved differential mode is only 9.7% of that in the normal MIP mode. The improved differential strategy can be used in other MIP-based sensors with semi-logarithmic calibration curves.

In this work, a super-sensitive electrochemiluminescence (ECL) aptamer sensor was constructed using a multiple signal amplification strategy to realize ultra-sensitive detection of di-(2-ethylhexyl) phthalate (DEHP). The incorporation of a highly efficient electrocatalytic metal-organic framework (NH2-Zr-MOF) and graphdiyne (GDY) composite has significantly enhanced the overall electrochemically active surface area, facilitating electron transfer during the entire electrochemical reaction process, and the large number of pores in graphdiyne and NH2-Zr-MOF limited a series of redox reactions within a certain range. This resulted in the generation of a greater number of SO4˙- radicals, thereby boosting the ECL intensity of the GDY in the K2S2O8 system. To increase the performance of the sensor even further, sodium ascorbate (NaAsc) as an accelerator was added to the co-reactant system. Additionally, nitrogen micro-nano bubbles with higher stability and stronger mass transfer have been introduced into the ECL system for the first time. Based on these, the aptamer as the recognition element realized the ultra-sensitive detection of DEHP in the linear range of 1.0 × 10-12 to 1.0 × 10-4 mg mL-1 with the limit of detection (LOD) of 2.43 × 10-13 mg mL-1. In summary, we have utilized the electrocatalytic activity of the porous MOF and the reducing capability of sodium ascorbate to enhance the ECL emission of GDY, which has been successfully applied to the detection of DEHP in water samples.

Herein, a Faraday cage-type electrochemiluminescence biosensor was designed for the detection of human breast cancer cell MCF-7. Two kinds of nanomaterials, Fe₃O₄-APTs and GO@PTCA-APTs, were synthesized as capture unit and signal unit, respectively. In presence of the target MCF-7, the Faraday cage-type electrochemiluminescence biosensor was constructed by forming a complex "capture unit-MCF-7-signal unit". In this case, lots of electrochemiluminescence signal probes were assembled and could participate in the electrode reaction, achieving a significant increase in sensitivity. In addition, the double aptamer recognition strategy was adopted to improve the capture, enrichment efficiency and detection reliability. Under optimal experimental conditions, the limit of detection was 3 cells/mL. And, the sensor could afford the detection of actual human blood samples, which is the first report on the detection of intact circulating tumor cells by the Faraday cage-type electrochemiluminescence biosensor.

At the nanoscale, metals exhibit special electrochemical and optical properties, which play an important role in nanobiosensing. In particular, surface plasmon resonance (SPR) based on precious metal nanoparticles, as a kind of tag-free biosensor technology, has brought high sensitivity, high reliability, and convenient operation to sensor detection. By applying an electrochemical excitation signal to the nanoplasma device, modulating its surface electron density, and realizing electrochemical coupling SPR, it can effectively complete the joint transmission of electrical and optical signals, increase the resonance shift of the spectrum, and further improve the sensitivity of the designed biosensor. In addition, smartphones are playing an increasingly important role in portable mobile sensor detection systems. These systems typically connect sensing devices to smartphones to perceive different types of information, from optical signals to electrochemical signals, providing ideas for the portability and low-cost design of these sensing systems. Among them, electrochemiluminescence (ECL), as a special electrochemically coupled optical technology, has good application prospects in mobile sensing detection due to its strong anti-interference ability, which is not affected by background light. In this review, the SPR is introduced using nanoparticles, and its response process is analyzed theoretically. Then, the mechanism and sensing application of electrochemistry coupled with SPR and ECL are emphatically introduced. Finally, it extends to the relevant research on electrochemically coupled optical sensing on mobile detection platforms.

Ensuring food safety continues to be one of the major global challenges. For effective food safety monitoring, fast, sensitive, portable, and efficient food safety detection strategies must be devised. Metal organic frameworks (MOFs) are porous crystalline materials that have attracted attention for use in high-performance sensors for food safety detection owing to their advantages such as high porosity, large specific surface area, adjustable structure, and easy surface functional modification. Immunoassay strategies based on antigen-antibody specific binding are one of the important means for accurate and rapid detection of trace contaminants in food. Emerging MOFs and their composites with excellent properties are being synthesized, providing new ideas for immunoassays. This article summarizes the synthesis strategies of MOFs and MOF-based composites and their applications in the immunoassays of food contaminants. The challenges and prospects of the preparation and immunoassay applications of MOF-based composites are also presented. The findings of this study will contribute to the development and application of novel MOF-based composites with excellent properties and provide insights into advanced and efficient strategies for developing immunoassays.

Exosomes play an important role in the proliferation, adhesion and migration of cancer cells. In this study, we have developed a novel electrochemiluminescence (ECL) sensor based on MoS2 QDs-MXene heterostructure and Au NPs@biomimetic lipid layer to detect exosomal miRNA. MoS2 QDs-MXene heterostructure had been prepared as the luminescence probe. Ti3C2Tx MXene nanosheets possessed the large specific surface area, excellent flexibility and superior conductivity. MoS2 QDs on the MXene nanosheets worked as the radiation center to generate strong ECL signal. Meanwhile, Au NPs with biomimetic lipid layer have been modified on the electrode, which retained the lipid dynamics and excellent antifouling property. When miRNA-135b was recognized on the Au NPs@biomimetic lipid layer, MoS2 QDs-MXene heterostructure was linked on the electrode and further extended the outer Helmholtz plane. As a result, the self-luminous Faraday cage-mode sensing system has been used to detect miRNA-135b from 30 fM to 20 nM with a detection limit of 10 fM. Furthermore, gastric cancer exosomal miRNA in the ascites of clinical patients has been detected successfully. The sensing system can be served as a versatile platform with huge application potential in the field of exosome detection.

In this work, a novel electrochemiluminescence (ECL) sensor has been developed to detect the miRNA-522 in the tumor tissues of triple-negative breast cancer (TNBC) patients. Au NPs/Zn MOF heterostructure was obtained by in situ growth and used as novel luminescence probe. Firstly, zinc-metal organic framework nanosheets (Zn MOF NSs) were synthesized with Zn²⁺ as the central metal ion and 2-aminoterephthalic acid (NH₂-BDC) as the ligand. 2D MOF nanosheets with ultra-thin layered structure and relatively large specific surface areas can enhance the catalytic activity in the ECL generation. Furthermore, the electron transfer capacity and the electrochemical active surface area of MOF were greatly improved by the growth of Au NPs. Therefore, Au NPs/Zn MOF heterostructure showed the significant electrochemical activity in the sensing process. In addition, the magnetic Fe₃O₄@SiO₂@Au microspheres were used as capture units in the magnetic separation step. The magnetic spheres with hairpin aptamer H1 can capture target gene. Then the captured miRNA-522 triggered the target catalyzed hairpin assembly (CHA) sensing process and linked Au NPs/Zn MOF heterostructure. The concentration of miRNA-522 can be quantified by the ECL signal enhancement of the Au NPs/Zn MOF heterostructure. Due to the high catalytic activity of Au NPs/Zn MOF heterostructure and their unique structural and electrochemical properties, the prepared ECL sensor achieved high-sensitive detection of miRNA-522 in the range of 1 fM to 0.1 nM with the detection limit of 0.3 fM. This strategy can provide a potential alternative for miRNA detection in medical research and clinical diagnosis of triple negative breast cancer.

The ECL emission of simple and stable zirconium dioxide nanomaterials has always been a blank slate in the ECL sensors field. In this work, zirconium dioxide (ZrO₂)-titanium dioxide (TiO₂)-gold nanoparticle (AuNPs) composite (ZT-Au), a novel self-enhanced ECL emitter, was introduced the system of dual-quenching ECL immunosensor. The anodic luminescence of ZrO₂ in the system of tripropylamine (TPrA) as a co-reagent was first reported and explored. Meanwhile, TiO₂ was designed into the ECL scheme as a co-reaction accelerator to form the ZrO₂/TPrA/TiO₂ ternary system, which can efficiently amplify the ECL signal of the emitter. In addition, cuprous oxide-triaminophenol (Cu₂O-APF) as the quencher was devoted to the dual-quenching sensing strategy. The dual-quenching mechanism that effectively boosted the immunosensor sensitivity was adequately investigated and conjectured in this paper. The sensing model based on the luminophor ZT-Au and the quencher Cu₂O-APF was utilized for the detection of D-dimer, a reliable marker for the diagnosis and evaluation of thrombotic diseases. The short peptide ligands NARKFYKGC (NFC) with efficient biological affinity were used to site-directionally capture antibodies for adequately protecting the activity of antigen binding sites during the construction of the immunosensor. The implemented immunosensor was equipped with a broad linear range of 0.01-500 ng/mL and a low detection limit of 3.6 pg/mL. The original methodology opens up the field of vision for the detection of additional biomarkers.

To develop an ultra-sensitive solid-state electrochemiluminescence (ECL) biosensor for detection of miRNA 24, three different forms of porphyrin metal-organic framework (MOF) nanomaterials with good biocompatibility were synthesized through small molecule ligand modulation. We investigated various properties of synthesized MOFs in the presence of different small molecule ligands. The as-obtained 2D MOF nanodisk exhibited high ECL intensity and outstanding stability in the presence of a co-reactant at low concentrations. We also synthesized zinc-based quantum dots (Zn-NGQDs) with excellent photovoltaic properties by doping zinc dithiothreitol (DTT-Zn) into quantum dots. Accordingly, an enzyme-free solid-state ECL biosensor for miRNA 24 based on the "on-off-on" signal conversion strategy was created. Dependent on the synergy between the luminophor 2D MOF and Zn-NGQDs, the biosensor achieves a wide linear range from 1.00 × 10^-16 to 1.00 × 10^-10 mol·L^-1 and an exceedingly low detection limit of 0.03 fM. Furthermore, the ECL biosensor exhibits outstanding selectivity, repeatability, and stability. The method has great potential for investigating sensitive detection models for various biomolecules and the design of highly efficient MOF luminescent materials.

New technologies have provided suitable tools for rapid diagnosis of cancer which can reduce treatment costs and even increase patients' survival rates. Recently, the development of electrochemical aptamer-based nanobiosensors has raised great hopes for early, sensitive, selective, and low-cost cancer diagnosis. Here, we reviewed the flagged recent research (2021-2023) developed as a series of biosensors equipped with nanomaterials and aptamer sequences (nanoaptasensors) to diagnose/prognosis of various types of cancers. Equipping these aptasensors with nanomaterials and using advanced biomolecular technologies have provided specified biosensing interfaces for more optimal and reliable detection of cancer biomarkers. The primary intention of this review was to present and categorize the latest innovations used in the design of these diagnostic tools, including the hottest surface modifications and assembly of sensing bioplatforms considering diagnostic mechanisms. The main classification is based on applying various nanomaterials and sub-classifications considered based on the type of analyte and other vital features. This review may help design subsequent electrochemical aptasensors. Likewise, the up-to-date status, remaining limitations, and possible paths for translating aptasensors to clinical cancer assay tools can be clarified.

Pressure has been a facile signal readout mode for developing point-of-care testing devices due to the attractive features of portability, accessibility, rapidity, and affordability. Herein, a pressure signal readout device was designed by integrating two homemade needle-type piezoresistive transducers, a controller for a thin-film piezoresistive sensor and a smartphone. Meanwhile, a bidirectional immunochromatographic test strip was designed as an immunoreaction platform for dual-analyte detection. Using PdCuPt nanoparticles with catalase-mimic activity as signal tags, the pressure signals triggered by catalyzed aerogenous reaction were monitored by the pressure signal readout device and read on a smartphone with the Bluetooth module. In this proof-of-principle work, imidacloprid and carbendazim were detected as model analytes. The dynamic ranges for quantitating imidacloprid and carbendazim are 20 pg mL^-1 to 50 ng mL^-1 and 50 pg mL^-1 to 50 ng mL^-1, respectively. The whole immunoassay process was completed within 16 min. The recovery values for imidacloprid and carbendazim spiked into herbal medicines are 82.0-110.0 and 84.0-116.0%, respectively, verifying its reliability for real sample detection. As the smartphone APP and controller for a thin-film piezoresistive sensor contain 12 signal channels, the system can be easily extended to meet the demand for high-throughput screening.

Recent years have witnessed the emergence of innovative analytical methods with high sensitivity and spatiotemporal resolution that allowed qualitative and quantitative analysis to be carried out at single-cell and subcellular levels. Electrochemiluminescence (ECL) is a unique chemiluminescence of high-energy electron transfer triggered by electrical excitation. The ingenious combination of electrochemistry and chemiluminescence results in the distinct advantages of high sensitivity, a wide dynamic range and good reproducibility. Specifically, single-cell ECL (SCECL) analysis with excellent spatiotemporal resolution has emerged as a promising toolbox in bioanalysis for revealing individual cells' heterogeneity and stochastic processes. This review focuses on advances in SCECL analysis and bioimaging. The history and recent advances in ECL probes and strategies for system design are briefly reviewed. Subsequently, the latest advances in representative SCECL analysis techniques for bioassays, bioimaging and therapeutics are also highlighted. Then, the current challenges and future perspectives are discussed.

Taking advantage of high porosity, large surface area, tunable nanostructures and ease of functionalization, metal-organic frameworks (MOFs) have been popularly applied in different fields, including adsorption and separation, heterogeneous catalysis, drug delivery, light harvesting, and chemical/biological sensing. The abundant active sites for specific recognition and adjustable optical and electrical characteristics allow for the design of various sensing platforms with MOFs as promising candidates. In this review, we systematically introduce the recent advancements of MOFs-based fluorescent chemosensors and biosensors, mainly focusing on the sensing mechanisms and analytes, including inorganic ions, small organic molecules and biomarkers (e.g., small biomolecules, nucleic acids, proteins, enzymes, and tumor cells). This review may provide valuable references for the development of novel MOFs-based sensing platforms to meet the requirements of environment monitoring and clinical diagnosis.