Copyright
©The Author(s) 2016.
World J Ophthalmol. Aug 12, 2016; 6(3): 20-27
Published online Aug 12, 2016. doi: 10.5318/wjo.v6.i3.20
Published online Aug 12, 2016. doi: 10.5318/wjo.v6.i3.20
Figure 4 Extracellular signal-regulated kinase-1/2 activation in cultured primary human ciliary muscle cells.
Effects of two B2-receptor antagonists on kinin-induced ERK1/2 activation by three BK agonists are shown in (A). Effect of bromfenac on kinin-mediated ERK1/2 activation are shown in (B). Serum-deprived h-CM cells were incubated for 10 min with 100 nmol/L B2 selective agonists BK, Met-Lys-BK and RMP-7. In experiments with antagonists or bromfenac, cells were pretreated with 100 nmol/L B2 receptor antagonist HOE-140 or WIN-64338 or bromfenac (1 μmol/L) for 15 min prior to addition of BK agonists. At the end of incubation, cell lysates were analyzed for phosphorylated ERK1/2 using Cellul’erk kit from CisBio. Significantly different than basal responses, aP < 0.05 by Student’s t-test. BK: Bradykinin; ERK1/2: Extracellular signal-regulated kinase-1/2; h-CM: Human ciliary muscle; BF: Bromfenac.
- Citation: Sharif NA, Patil R, Li L, Husain S. Human ciliary muscle cell responses to kinins: Activation of ERK1/2 and pro-matrix metalloproteinases secretion. World J Ophthalmol 2016; 6(3): 20-27
- URL: https://www.wjgnet.com/2218-6239/full/v6/i3/20.htm
- DOI: https://dx.doi.org/10.5318/wjo.v6.i3.20