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Speroni S, Polizzi E. Green Dentistry: State of the Art and Possible Development Proposals. Dent J (Basel) 2025; 13:38. [PMID: 39851612 PMCID: PMC11764173 DOI: 10.3390/dj13010038] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/22/2024] [Revised: 11/19/2024] [Accepted: 12/19/2024] [Indexed: 01/26/2025] Open
Abstract
Objectives: The objective of this narrative literature review was to highlight all dental procedures attributable to sectoral waste and to consider possible alternatives in line with the concept of sustainable development. Methods: An extensive search of electronic databases, including the Cochrane Oral Health Group Specialized Register, Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science, PubMed, EMBASE, and Google Scholar. Search words included 'Green Dentistry', 'Dental Pollution', 'Pollutants and Dentistry', 'Disinfectants and Dentistry', and 'High-tech Dentistry'. All of them allowed an assessment of the impact of dental practice on the external environment, and new frontiers currently applied or possibly applicable for green dentistry were included in the study. Non-full-text papers, animal studies, studies in languages other than English, and studies not related to the topic under consideration were excluded. Results: According to the inclusion criteria, 76 papers were selected for the study. The topics analyzed were the impact of dental practice on the outdoor environment, currently applied and potentially applicable principles of green dentistry, and the 'Four Rs' model (Rethink, Reduce, Reuse, and Recycle). Conclusions: With the limitations of the present study, the concept of green dentistry could be applicable provided that the measures already taken to reduce indoor and outdoor risk factors are continued and improved.
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Affiliation(s)
- Stefano Speroni
- Department of Dentistry, Dental School, IRCCS San Raffaele Hospital, Vita-Salute San Raffaele University, 20132 Milan, Italy;
| | - Elisabetta Polizzi
- Chair Center for Oral Hygiene and Prevention, Department of Dentistry, Dental School, IRCCS San Raffaele Hospital, Vita-Salute San Raffaele University, 20132 Milan, Italy
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Bai Y, Zhou Y, Chang R, Hu X, Zhou Y, Chen J, Zhang Z, Yao J. Transcription profiles and phenotype reveal global response of Staphylococcus aureus exposed to ultrasound and ultraviolet stressors. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 912:169146. [PMID: 38061661 DOI: 10.1016/j.scitotenv.2023.169146] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/24/2023] [Revised: 11/05/2023] [Accepted: 12/04/2023] [Indexed: 12/19/2023]
Abstract
Ultrasound and ultraviolet light have good inactivation performance against pathogens in sewage. In this study, the inactivation mechanisms of 60 kHz ultrasound and ultraviolet radiation against Staphylococcus aureus (S. aureus) were studied from the perspectives of cell phenotype and transcriptome for the first time. The results showed that both ultrasound and ultraviolet treatments had adverse impacts on the cellular morphology of S. aureus to varying degrees at cellular level. The transcriptomic analysis revealed that there were 225 and 1077 differentially expressed genes (DEGs) in the ultrasound and ultraviolet treatments, respectively. The result revealed that both ultrasound and ultraviolet could interfere with the expression of the genes involved in ABC transporters, amino acid and fatty acid metabolism to influence the membrane permeability. Besides the membrane permeability, ultraviolet also could disturb the ATP synthesis, DNA replication and cell division through restraining the expression of several genes related to carbohydrate metabolism, peptidoglycan synthesis, DNA-binding/repair protein synthesis. Compared with the single inactivation pathway of ultrasound, ultraviolet inactivation of S. aureus is multi-target and multi-pathway. We believe that the bactericidal mechanisms of ultrasound and ultraviolet radiation presented by this study could provide theoretical guidance for the synergistic inactivation of pathogens in sewage by ultrasound and ultraviolet radiation in the future.
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Affiliation(s)
- Yun Bai
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Yuanhang Zhou
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Ruiting Chang
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Xueli Hu
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Yingying Zhou
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Jiabo Chen
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China
| | - Zhi Zhang
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
| | - Juanjuan Yao
- Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
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Lin Y, He Y, Sun Q, Ping Q, Huang M, Wang L, Li Y. Underlying the mechanisms of pathogen inactivation and regrowth in wastewater using peracetic acid-based disinfection processes: A critical review. JOURNAL OF HAZARDOUS MATERIALS 2024; 463:132868. [PMID: 37944231 DOI: 10.1016/j.jhazmat.2023.132868] [Citation(s) in RCA: 2] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/27/2023] [Revised: 10/17/2023] [Accepted: 10/24/2023] [Indexed: 11/12/2023]
Abstract
Peracetic acid (PAA) disinfection is an emerging wastewater disinfection process. Its advantages include excellent pathogen inactivation performance and little generation of toxic and harmful disinfection byproducts. The objective of this review is to comprehensively analyze the experimental data and scientific information related to PAA-based disinfection processes. Kinetic models and modeling frameworks are discussed to provide effective tools to assess pathogen inactivation efficacy. Then, the efficacy of PAA-based disinfection processes for pathogen inactivation is summarized, and the inactivation mechanisms involved in disinfection and the interactions of PAA with conventional disinfection processes are elaborated. Subsequently, the risk of pathogen regrowth after PAA-based disinfection process is clearly discussed. Finally, to address ecological risks related to PAA-based disinfection, its impact on the spread of antibiotic-resistant bacteria and the transfer of antibiotic resistance genes (ARGs) is also assessed. Among advanced PAA-based disinfection processes, ultraviolet/PAA is promising not only because it has practical application value but also because pathogen regrowth can be inhibited and ARGs transfer risk can be significantly reduced via this process. This review presents valuable and comprehensive information to provide an in-depth understanding of PAA as an alternative wastewater disinfection technology.
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Affiliation(s)
- Yuqian Lin
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China
| | - Yunpeng He
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China
| | - Qiya Sun
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China
| | - Qian Ping
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, People's Republic of China
| | - Manhong Huang
- Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, People's Republic of China; Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, College of Environmental Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China
| | - Lin Wang
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, People's Republic of China.
| | - Yongmei Li
- State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, People's Republic of China
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Liu B, Jun Y, Zhao C, Zhou C, Zhu T, Shao S. Using Fe(II)/Fe(VI) activated peracetic acid as pretreatment of ultrafiltration for secondary effluent treatment: Water quality improvement and membrane fouling mitigation. WATER RESEARCH 2023; 244:120533. [PMID: 37659184 DOI: 10.1016/j.watres.2023.120533] [Citation(s) in RCA: 8] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/13/2023] [Revised: 08/25/2023] [Accepted: 08/26/2023] [Indexed: 09/04/2023]
Abstract
Ultrafiltration (UF) is a technology commonly used to treat secondary effluents in wastewater reuse; however, it faces two main challenges: 1) membrane fouling and 2) inadequate nitrogen (N), phosphorus (P), and organic micropollutants (OMPs) removal. To address these two issues, in this study, we applied peracetic acid (PAA), Fe(VI)/PAA, and Fe(II)/PAA as UF pretreatments. The results showed that the most effective pretreatment was Fe(II)/200 μM PAA, which reduced the total fouling resistance by 90.2%. In comparison, the reduction was only 29.7% with 200 μM PAA alone and 64.3% with Fe(VI)/200 μM PAA. Fe(II)/200 μM PAA could effectively remove fluorescent components and hydrophobic organics in effluent organic matter (EfOM), and enhance the repulsive force between foulants and membrane (according to XDLVO analysis), and consequently, mitigate pore blocking and delay cake layer formation. Regarding pollutant removal, Fe(II)/200 μM PAA effectively degraded OMPs (>85%) and improved P removal by 58.2% via in-situ Fe(Ⅲ) co-precipitation. The quencher and probe experiments indicated that FeIVO2+, •OH, and CH3C(O)OO•/CH3C(O)O• all played important roles in micropollutant degradation with Fe(II)/PAA. Interestingly, PAA oxidation produced highly biodegradable products such as acetic acid, which significantly elevated the BOD5 level and increased the BOD5/total nitrogen (BOD5/TN) ratio from 0.8 to 8.6, benefiting N removal with subsequent denitrification. Overall, the Fe(II)/PAA process exhibits great potential as a UF pretreatment to control membrane fouling and improve water quality during secondary effluent treatment.
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Affiliation(s)
- Bin Liu
- Hunan Engineering Research Center of Water Security Technology and Application, College of Civil Engineering, Hunan University, Changsha 410082, PR China
| | - Yin Jun
- Hunan Engineering Research Center of Water Security Technology and Application, College of Civil Engineering, Hunan University, Changsha 410082, PR China
| | - Changrong Zhao
- Hunan Engineering Research Center of Water Security Technology and Application, College of Civil Engineering, Hunan University, Changsha 410082, PR China
| | - Chu Zhou
- School of Civil Engineering, Wuhan University, Wuhan 430072, PR China
| | - Tingting Zhu
- Hunan Engineering Research Center of Water Security Technology and Application, College of Civil Engineering, Hunan University, Changsha 410082, PR China.
| | - Senlin Shao
- School of Civil Engineering, Wuhan University, Wuhan 430072, PR China.
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Rhee CH, Lee HS, Yun HJ, Lee GH, Kim SJ, Song S, Lee MH, Her M, Jeong W. Chemical stability of active ingredients in diluted veterinary disinfectant solutions under simulated storage conditions. Front Chem 2023; 11:1204477. [PMID: 37398983 PMCID: PMC10311561 DOI: 10.3389/fchem.2023.1204477] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2023] [Accepted: 06/07/2023] [Indexed: 07/04/2023] Open
Abstract
Introduction: The product labels of veterinary disinfectants specify their expiration dates to prevent the use of outdated products, as these may result in disinfection and biosecurity failures during outbreak situations. However, a clear standard for the storage conditions of diluted disinfectant solutions has not yet been established, and the effects of storage conditions have scarcely been investigated. To fill this research gap, our study examined the stability of the active ingredients of diluted veterinary disinfectants based on their change in concentrations when stored at various temperatures for various time periods. Methods: Twenty veterinary disinfectants effective against either foot-and-mouth disease or avian influenza viruses were selected. The disinfectants were diluted to effective concentrations following the manufacturer's instructions. Using selective analytical techniques, the concentrations of the active ingredients of the samples that had been stored for varying intervals at different temperatures (4, 20, 30, and 45°C) were determined. These samples included soaps and detergents, acids, oxidizing agents, aldehydes, and copper compounds. The active ingredient concentrations of two of the samples were determined following freezing/thawing cycle, to establish their stability when exposed to simulated winter conditions. Results: Our results showed that most of the active ingredients had concentrations of 90% or greater of their initial concentrations, indicating ≥90% stability over a 21-day period under the experimental storage conditions. However, there were some exceptions. Glutaraldehyde, formaldehyde, and malic acid are over 90% stable at ≤ 30°C for 21 days, but their concentrations decreased to below 90% of their initial concentrations at 45°C, indicating a decline in stability when stored at 45°C for 21 days. The concentrations of potassium peroxymonosulfate and peracetic acid rapidly declined with increasing time and temperature to less than 90% of their initial concentrations. Discussion: Based on our findings, we propose that diluted disinfectant solutions should preferably be prepared daily. However, if the daily preparation of a diluted disinfectant solution is not feasible, then our results can be used as a reference, providing basic scientific data on the chemical stability of diluted disinfectant solutions commonly used in the veterinary field, thus indicating suitable storage conditions.
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Affiliation(s)
- Chae Hong Rhee
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
- Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea
| | - Hye-sook Lee
- Korea Animal Health Products Association, Seongnam-si, Gyeonggi-do, Republic of Korea
| | - Hyeong-jun Yun
- Korea Animal Health Products Association, Seongnam-si, Gyeonggi-do, Republic of Korea
| | - Ga-Hee Lee
- Korea Animal Health Products Association, Seongnam-si, Gyeonggi-do, Republic of Korea
| | - Su-Jeong Kim
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
| | - Sok Song
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
| | - Myoung-Heon Lee
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
| | - Moon Her
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
| | - Wooseog Jeong
- Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea
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Maione A, Galdiero E, Cirillo L, Gambino E, Gallo MA, Sasso FP, Petrillo A, Guida M, Galdiero M. Prevalence, Resistance Patterns and Biofilm Production Ability of Bacterial Uropathogens from Cases of Community-Acquired Urinary Tract Infections in South Italy. Pathogens 2023; 12:pathogens12040537. [PMID: 37111423 PMCID: PMC10145297 DOI: 10.3390/pathogens12040537] [Citation(s) in RCA: 10] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/23/2023] [Revised: 03/23/2023] [Accepted: 03/28/2023] [Indexed: 04/29/2023] Open
Abstract
Community-acquired urinary tract infections represent the most common infectious diseases in the community setting. Knowing the antibiotic resistance patterns of uropathogens is crucial for establishing empirical treatment. The aim of the current study is to determine the incidence of the causative agents of UTIs and their resistance profiles. Patients of all ages and both sexes were enrolled in the study, and admitted to San Ciro Diagnostic Center in Naples between January 2019 and Jun 2020. Bacterial identification and antibiotic susceptibility testing were carried out using Vitek 2 system. Among the 2741 urine samples, 1702 (62.1%) and 1309 (37.9%) were negative and positive for bacterial growth, respectively. Of 1309 patients with infection, 760 (73.1%) were females and 279 (26.9%) were males. The greatest number of positive cases were found in the in the elderly (>61 years). Regarding uropathogens, 1000 (96.2%) were Gram-negative while 39 (3.8%) were Gram-positive strains. The three most isolated pathogenic strains were Escherichia coli (72.2%), Klebsiella pneumoniae (12.4%), and Proteus mirabilis (9.0%). Strong biofilm formation ability was observed in about 30% of the tested isolates. The low resistance rates recorded against nitrofurantoin, fosfomycin, piperacillin-tazobactam, and gentamicin could suggest them as the most appropriate therapies for CA-UTIs.
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Affiliation(s)
- Angela Maione
- Department of Biology, University of Naples 'Federico II', Via Cinthia, 80126 Naples, Italy
| | - Emilia Galdiero
- Department of Biology, University of Naples 'Federico II', Via Cinthia, 80126 Naples, Italy
| | - Luigi Cirillo
- Department of Neurosciences, Reproductive Sciences and Odontostomatology, University of Naples "Federico II", 80131 Naples, Italy
| | - Edvige Gambino
- Department of Biology, University of Naples 'Federico II', Via Cinthia, 80126 Naples, Italy
| | | | - Francesca Paola Sasso
- Department of Dermatology and Venereology, University of Rome "La Sapienza", 00161 Rome, Italy
| | | | - Marco Guida
- Department of Biology, University of Naples 'Federico II', Via Cinthia, 80126 Naples, Italy
| | - Marilena Galdiero
- Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 81100 Naples, Italy
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Folliero V, Ricciardi M, Dell’Annunziata F, Pironti C, Galdiero M, Franci G, Motta O, Proto A. Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens. TOXICS 2022; 10:768. [PMID: 36548601 PMCID: PMC9780819 DOI: 10.3390/toxics10120768] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 11/03/2022] [Revised: 12/03/2022] [Accepted: 12/05/2022] [Indexed: 06/17/2023]
Abstract
BACKGROUND The disinfection process represents an important activity closely linked to the removal of micro-organisms in common processing systems. Traditional disinfectants are often not sufficient to avoid the spread of food pathogens; therefore, innovative strategies for decontamination are crucial to countering microbial transmission. This study aims to assess the antimicrobial efficiency of tetrapotassium iminodisuccinic acid salt (IDSK) against the most common pathogens present on surfaces, especially in food-borne environments. METHODS IDSK was synthesized from maleic anhydride and characterized through nuclear magnetic resonance (NMR) spectroscopy (both 1H-NMR and 13C-NMR), thermogravimetric analysis (TGA) and Fourier Transform Infrared (FTIR) spectroscopy. The antibacterial activity was performed via the broth microdilution method and time-killing assays against Escherichia coli, Staphylococcus aureus, Salmonella enterica, Enterococcus faecalis and Pseudomonas aeruginosa (IDSK concentration range: 0.5-0.002 M). The biofilm biomass eradicating activity was assessed via a crystal violet (CV) assay. RESULTS The minimum inhibitory concentration (MIC) of IDSK was 0.25 M for all tested strains, exerting bacteriostatic action. IDSK also reduced biofilm biomass in a dose-dependent manner, reaching rates of about 50% eradication at a dose of 0.25 M. The advantages of using this innovative compound are not limited to disinfecting efficiency but also include its high biodegradability and its sustainable synthesis. CONCLUSIONS IDSK could represent an innovative and advantageous disinfectant for food processing and workers' activities, leading to a better quality of food and safer working conditions for the operators.
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Affiliation(s)
- Veronica Folliero
- Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, Italy
| | - Maria Ricciardi
- Department of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132-84084 Fisciano, Italy
| | - Federica Dell’Annunziata
- Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, Italy
| | - Concetta Pironti
- Department of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, Italy
| | - Massimiliano Galdiero
- Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, Italy
| | - Gianluigi Franci
- Department of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, Italy
| | - Oriana Motta
- Department of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, Italy
| | - Antonio Proto
- Department of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132-84084 Fisciano, Italy
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Chen X, Chen Z, Liu H, Huang N, Mao Y, Cao K, Shi Q, Lu Y, Hu HY. Synergistic effects of UV and chlorine in bacterial inactivation for sustainable water reclamation and reuse. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 845:157320. [PMID: 35839898 DOI: 10.1016/j.scitotenv.2022.157320] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/18/2022] [Revised: 06/30/2022] [Accepted: 07/08/2022] [Indexed: 06/15/2023]
Abstract
Disinfection is a necessity in water and wastewater treatment and reclamation. This study examined the inactivation of a disinfectant resistant but widely existed opportunistic pathogen in reclaimed water, Staphylococcus aureus (S. aureus), by sequential UV and chlorine disinfection or simultaneous UV and chlorine disinfection (UV/Cl). It was identified that UV/Cl greatly promoted the inactivation efficacy and inhibited photoreactivation of S. aureus by the generation of free radicals (i.e. OH and Cl), which reached a 7-log10 reduction at UV and chlorine doses of 18 mJ/cm2 and 2 mg-Cl/L, respectively. The changes of bacterial viability and morphology and the increase of extracellular ATP concentration confirmed the enhancement of cell membranes damages (>21.4 %) due to free radicals generated in UV/Cl process, which caused a dramatic reduction in metabolic activity and suppressed the photoreactivation. Furthermore, this study demonstrated that UV/Cl effectively removed heterotrophic plate count bacteria and aromatic organic fluorophores in reclaimed water samples. This study is of significant theoretical and applicable importance in guaranteeing safe microbial levels for water reclamation and reuse.
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Affiliation(s)
- Xiaowen Chen
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China
| | - Zhuo Chen
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Beijing Laboratory for Environmental Frontier Technologies, Beijing 100084, PR China.
| | - Hai Liu
- School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, PR China
| | - Nan Huang
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China
| | - Yu Mao
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China
| | - Kefan Cao
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China
| | - Qi Shi
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China
| | - Yun Lu
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Beijing Laboratory for Environmental Frontier Technologies, Beijing 100084, PR China
| | - Hong-Ying Hu
- Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Beijing Laboratory for Environmental Frontier Technologies, Beijing 100084, PR China; Research Institute for Environmental Innovation (Suzhou), Tsinghua, Jiangsu, Suzhou 215163, PR China
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9
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Zhao Y, Zhao Y, Yu X, Kong D, Fan X, Wang R, Luo S, Lu D, Nan J, Ma J. Peracetic acid integrated catalytic ceramic membrane filtration for enhanced membrane fouling control: Performance evaluation and mechanism analysis. WATER RESEARCH 2022; 220:118710. [PMID: 35687976 DOI: 10.1016/j.watres.2022.118710] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/01/2022] [Revised: 05/22/2022] [Accepted: 05/31/2022] [Indexed: 05/09/2023]
Abstract
Endowing ceramic membrane (CM) catalytic reactivity can enhance membrane fouling control in the aid of in situ oxidation process. Peracetic acid (PAA) oxidant holds great prospect to integrate with CM for membrane fouling control, owing to the prominent advantages of high oxidation efficacy and easy activation. Herein, this study, for the first time, presented a PAA/CM catalytic filtration system achieving highly-efficient protein fouling alleviation. A FeOCl functionalized CM (FeOCl-CM) was synthesized, possessing high hydrophilicity, low surface roughness, and highly-efficient activation towards PAA oxidation. Using bovine serum albumin (BSA) as the model protein foulant, the PAA/FeOCl-CM catalytic filtration notably alleviated fouling occurring in both membrane pores and surface, and halved the flux reduction degree as compared with the conventional CM filtration. The PAA/FeOCl-CM catalytic oxidation allows quick and complete disintegration of BSA particles, via the breakage of the amide I and II bands and the ring opening of the aromatic amino acids (e.g., Tryptophan, Tyrosine). In-depth investigation revealed that the in situ generated •OH and 1O2 were the key reactive species towards BSA degradation during catalytic filtration, while the organic radical oxidation and the direct electron transfer pathway from BSA to PAA via FeOCl-CM played minor roles. Overall, our findings highlight a new PAA/CM catalytic filtration strategy for achieving highly-efficient membrane fouling control and provide an understanding of the integrated PAA catalytic oxidation - membrane filtration behaviors.
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Affiliation(s)
- Yumeng Zhao
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Yanxin Zhao
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Xin Yu
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Dezhen Kong
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Xinru Fan
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Runzhi Wang
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Shuangjiang Luo
- Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
| | - Dongwei Lu
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
| | - Jun Nan
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
| | - Jun Ma
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
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10
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Impact of Escherichia coli Outer Membrane Vesicles on Sperm Function. Pathogens 2022; 11:pathogens11070782. [PMID: 35890027 PMCID: PMC9319964 DOI: 10.3390/pathogens11070782] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/25/2022] [Revised: 07/06/2022] [Accepted: 07/08/2022] [Indexed: 01/27/2023] Open
Abstract
Reproductive tract infections account for approximately 15% of male infertility cases. Escherichia coli (E. coli) represents the most frequently isolated bacterial strain in the semen of infertile men. All Gram-negative bacteria constitutively produce outer membrane vesicles (OMVs). The present study proved, for the first time, the involvement of OMVs in human sperm function. E. coli OMVs were isolated by ultracentrifugation and characterized via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Human sperm was exposed to OMVs (8 µg/mL) for different times (30, 45, 60 and 90 min). The vitality, motility, morphology, ROS level and DNA fragmentation of spermatozoa were evaluated. OMVs reduced the progressive motility and increased the immobile spermatozoa amount after 30 min of treatment. In addition, a significant increase in the percentage of intracellular ROS and sperm DNA fragmentation was recorded for each vesicular exposure time. These preliminary findings prove that OMVs contribute to altering human sperm function via two mechanisms: (i) impaired motility and (ii) DNA fragmentation.
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11
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Jiang S, Li Q, Jia W, Wang F, Cao X, Shen X, Yao Z. Expanding the application of ion exchange resins for the preparation of antimicrobial membranes to control foodborne pathogens. CHEMOSPHERE 2022; 295:133963. [PMID: 35167836 DOI: 10.1016/j.chemosphere.2022.133963] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/11/2021] [Revised: 02/09/2022] [Accepted: 02/10/2022] [Indexed: 06/14/2023]
Abstract
Although ion exchange resins (IERs) have been extensively adopted in water treatment, there are no reports on the application thereof for synthesizing antibacterial materials against pathogenic bacteria. The present study is the first in which the ion exchange characteristic of IERs was utilized to introduce silver ions that possess efficient antibacterial properties. The resulting antibacterial materials were incorporated into polylactic acid (PLA) and/or polybutylene adipate terephthalate (PBAT) to prepare antibacterial membranes. XPS spectra revealed the occurrence of in-situ reduction of silver ions to metallic silver, which was preferable since the stability of silver in the materials was improved. EDS mapping analysis indicated that the distribution of silver was consistent with the distribution of sulfur in the membranes, verifying the ion exchange methodology proposed in the present study. To investigate the antibacterial performance of the prepared membranes, zone of inhibition tests and bacteria-killing tests were performed. The results revealed that neither bare polymeric membranes of PLA and PBAT nor IER-incorporated polymeric membranes exhibited noticeable antibacterial activities. In comparison, the antibacterial membranes demonstrated effective and sustainable antibacterial activities against pathogenic bacteria Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The prepared antibacterial membranes exhibited potential in food-related applications such as food packaging to delay food spoilage due to microbial growth.
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Affiliation(s)
- Shanxue Jiang
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Qirun Li
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Wenting Jia
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Fang Wang
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Xinyue Cao
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Xianbao Shen
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China
| | - Zhiliang Yao
- School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China; State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing, 100048, China.
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12
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Folliero V, Dell’Annunziata F, Roscetto E, Amato A, Gasparro R, Zannella C, Casolaro V, De Filippis A, Catania MR, Franci G, Galdiero M. Rhein: A Novel Antibacterial Compound Against Streptococcus mutans Infection. Microbiol Res 2022; 261:127062. [DOI: 10.1016/j.micres.2022.127062] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/28/2021] [Revised: 05/04/2022] [Accepted: 05/04/2022] [Indexed: 12/15/2022]
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13
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Abstract
Polyphenolic esters (PEs) are valuable chemical compounds that display a wide spectrum of activities (e.g., anti-oxidative effects). As a result, their production through catalytic routes is an attractive field of research. The present review aims to discuss recent studies from the literature regarding the catalytic production of PEs from biomass feedstocks, namely, naturally occurred polyphenolic compounds. Several synthetic approaches are reported in the literature, mainly bio-catalysis and to a lesser extent acid catalysis. Immobilized lipases (e.g., Novozym 435) are the preferred enzymes thanks to their high reactivity, selectivity and reusability. Acid catalysis is principally investigated for the esterification of polyphenolic acids with fatty alcohols and/or glycerol, using both homogeneous (p-toluensulfonic acid, sulfonic acid and ionic liquids) and heterogeneous (strongly acidic cation exchange resins) catalysts. Based on the reviewed publications, we propose some suggestions to improve the synthesis of PEs with the aim of increasing the greenness of the overall production process. In fact, much more attention should be paid to the use of new and efficient acid catalysts and their reuse for multiple reaction cycles.
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14
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Investigation of biocidal efficacy of commercial disinfectants used in public, private and workplaces during the pandemic event of SARS-CoV-2. Sci Rep 2022; 12:5468. [PMID: 35361869 PMCID: PMC8969816 DOI: 10.1038/s41598-022-09575-1] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/15/2021] [Accepted: 03/25/2022] [Indexed: 12/24/2022] Open
Abstract
This study investigated the performance of 24 commercial disinfectants present on the market during last year according to the manufacturer’s instructions. Recently, national and international organizations of public health performed studies on disinfection products due to the increasing awareness of the potential and growing risks on human health, such as skin damage and reactions in the mucosal lining, especially for the healthcare workers in their frequent daily use. However, there are many limitations in the common cleaning/disinfection products on market as in the selection of effective disinfectants to decontaminate inanimate surfaces. We analyzed the disinfection power of hydrogen peroxide, quaternary ammonium compounds, alcohols, phenols and aldehydes used as active principles according to international guidelines. The antimicrobial properties were assessed by broth microdilution, and antibiofilm properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus); their virucidal efficacy was tested against Herpes simplex virus type 1 (HSV-1) and Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The quaternary ammonium compounds demonstrated better efficacy than others and in some cases ready to use products had also virucidal and antimicrobial activities after dilution at 0.125%. The scientific evidence indicates that many commercial products are used at high concentrations and high doses and this could have deleterious effects both on human health and the environment. A lower concentration of active ingredients would avoid the excessive release of chemicals into the environment and improve skin tolerance, ensuring the health and safety protection of workers, including the healthcare operators at their workplace.
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15
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Chinchkar AV, Singh A, Singh SV, Acharya AM, Kamble MG. Potential sanitizers and disinfectants for fresh fruits and vegetables: A comprehensive review. J FOOD PROCESS PRES 2022. [DOI: 10.1111/jfpp.16495] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
Affiliation(s)
- Ajay V. Chinchkar
- Department of Food Science and Technology National Institute of Food Technology Entrepreneurship and Management Kundli, Sonipat Haryana‐131028 India
| | - Anurag Singh
- Department of Food Science and Technology National Institute of Food Technology Entrepreneurship and Management Kundli, Sonipat Haryana‐131028 India
| | - Sukh Veer Singh
- Department of Food Science and Technology National Institute of Food Technology Entrepreneurship and Management Kundli, Sonipat Haryana‐131028 India
| | | | - Meenatai G. Kamble
- Department of Food Science and Technology National Institute of Food Technology Entrepreneurship and Management Kundli, Sonipat Haryana‐131028 India
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16
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Casein-derived peptides from the dairy product kashk exhibit wound healing properties and antibacterial activity against Staphylococcus aureus: structural and functional characterization. Food Res Int 2022; 153:110949. [DOI: 10.1016/j.foodres.2022.110949] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/22/2021] [Revised: 12/31/2021] [Accepted: 01/08/2022] [Indexed: 11/19/2022]
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17
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Hu J, Li T, Zhang X, Ren H, Huang H. Degradation of steroid estrogens by UV/peracetic acid: Influencing factors, free radical contribution and toxicity analysis. CHEMOSPHERE 2022; 287:132261. [PMID: 34555579 DOI: 10.1016/j.chemosphere.2021.132261] [Citation(s) in RCA: 15] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/24/2021] [Revised: 09/08/2021] [Accepted: 09/14/2021] [Indexed: 06/13/2023]
Abstract
Steroid estrogens (SEs) are a group of refractory organic micropollutants detected in secondary effluent frequently. The advanced oxidation processes (AOPs) are usually used to deep remove the SEs from the secondary effluent. Herein, we first investigated the UV/peracetic acid (PAA), a PAA-based AOP, to degrade SEs. Using estrone (E1), 17β-estradiol (E2), estriol (E3), and 17α-ethinyl estradiol (EE2) as representatives, the results showed that UV can effectively activate PAA to enhance the degradation of the four SEs, which degradation followed the pseudo-first-order kinetics (R2 > 0.99), and the rate constant (kobs) of degradation increased with increasing the PAA dosage in the range investigated. Little pH dependence was also observed in the degradation of SEs by UV/PAA. Furthermore, the degradation of SEs was improved in the presence of coexisting substrates (Cl-, HCO- 3, NO- 3, and HA) in relatively low concentrations. Quenching experiments revealed that the carbon-centered radicals (R-C•) produced from the UV/PAA process were recognized as the predominant contributors to the degradation of the four SEs. Also, we found that the estrogenic activity decreased by more than 94%, but the acute toxicity inhibition increased to 37% in the solution after 30 min UV/PAA treatment. In addition, the 130% additional total organic carbon (TOC) was generated after UV/PAA process. These findings obtained in this work will facilitate the development of the UV/PAA process as a promising strategy for the deep removal of SEs in secondary effluent.
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Affiliation(s)
- Jun Hu
- State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China
| | - Tong Li
- State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China
| | - Xuxiang Zhang
- State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China
| | - Hongqiang Ren
- State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China
| | - Hui Huang
- State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China.
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18
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Synthesis of Chitosan-Coated Silver Nanoparticle Bioconjugates and Their Antimicrobial Activity against Multidrug-Resistant Bacteria. APPLIED SCIENCES-BASEL 2021. [DOI: 10.3390/app11199340] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/24/2022]
Abstract
The increase in multidrug-resistant bacteria represents a true challenge in the pharmaceutical and biomedical fields. For this reason, research on the development of new potential antibacterial strategies is essential. Here, we describe the development of a green system for the synthesis of silver nanoparticles (AgNPs) bioconjugated with chitosan. We optimized a Prunus cerasus leaf extract as a source of silver and its conversion to chitosan–silver bioconjugates (CH-AgNPs). The AgNPs and CH-AgNPs were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible spectroscopy (UV–Vis), and zeta potential measurement (Z-potential). The cytotoxic activity of AgNPs and CH-AgNPs was assessed on Vero cells using the 3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The antibacterial activity of AgNPs and CH-AgNPs synthesized using the green system was determined using the broth microdilution method. We evaluated the antimicrobial activity against standard ATCC and clinically isolated multisensitive (MS) and multidrug-resistant bacteria (MDR) Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis), Klebsiella pneumonia (K. pneumoniae), and Staphylococcus aureus (S. aureus), using minimum inhibitory concentration (MIC) assays and the broth dilution method. The results of the antibacterial studies demonstrate that the silver chitosan bioconjugates were able to inhibit the growth of MDR strains more effectively than silver nanoparticles alone, with reduced cellular toxicity. These nanoparticles were stable in solution and had wide-spectrum antibacterial activity. The synthesis of silver and silver chitosan bioconjugates from Prunus cerasus leaf extracts may therefore serve as a simple, ecofriendly, noncytotoxic, economical, reliable, and safe method to produce antimicrobial compounds with low cytotoxicity.
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Dell’Annunziata F, Dell’Aversana C, Doti N, Donadio G, Dal Piaz F, Izzo V, De Filippis A, Galdiero M, Altucci L, Boccia G, Galdiero M, Folliero V, Franci G. Outer Membrane Vesicles Derived from Klebsiella pneumoniae Are a Driving Force for Horizontal Gene Transfer. Int J Mol Sci 2021; 22:ijms22168732. [PMID: 34445438 PMCID: PMC8395779 DOI: 10.3390/ijms22168732] [Citation(s) in RCA: 35] [Impact Index Per Article: 8.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/30/2021] [Revised: 08/11/2021] [Accepted: 08/12/2021] [Indexed: 12/17/2022] Open
Abstract
Gram-negative bacteria release Outer Membrane Vesicles (OMVs) into the extracellular environment. Recent studies recognized these vesicles as vectors to horizontal gene transfer; however, the parameters that mediate OMVs transfer within bacterial communities remain unclear. The present study highlights for the first time the transfer of plasmids containing resistance genes via OMVs derived from Klebsiella pneumoniae (K. pneumoniae). This mechanism confers DNA protection, it is plasmid copy number dependent with a ratio of 3.6 times among high copy number plasmid (pGR) versus low copy number plasmid (PRM), and the transformation efficiency was 3.6 times greater. Therefore, the DNA amount in the vesicular lumen and the efficacy of horizontal gene transfer was strictly dependent on the identity of the plasmid. Moreover, the role of K. pneumoniae-OMVs in interspecies transfer was described. The transfer ability was not related to the phylogenetic characteristics between the donor and the recipient species. K. pneumoniae-OMVs transferred plasmid to Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa and Burkholderia cepacia. These findings address the pivotal role of K. pneumoniae-OMVs as vectors for antimicrobial resistance genes spread, contributing to the development of antibiotic resistance in the microbial communities.
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Affiliation(s)
- Federica Dell’Annunziata
- Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy; (F.D.); (A.D.F.); (M.G.); (M.G.)
| | - Carmela Dell’Aversana
- Institute Experimental Endocrinology and Oncology “Gaetano Salvatore” (IEOS)-CNR, 80131 Naples, Italy;
- Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy;
| | - Nunzianna Doti
- Institute of Biostructures and Bioimaging (IBB), CNR, 80145 Naples, Italy;
| | - Giuliana Donadio
- Department of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84081 Salerno, Italy; (G.D.); (F.D.P.); (V.I.); (G.B.)
| | - Fabrizio Dal Piaz
- Department of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84081 Salerno, Italy; (G.D.); (F.D.P.); (V.I.); (G.B.)
| | - Viviana Izzo
- Department of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84081 Salerno, Italy; (G.D.); (F.D.P.); (V.I.); (G.B.)
| | - Anna De Filippis
- Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy; (F.D.); (A.D.F.); (M.G.); (M.G.)
| | - Marilena Galdiero
- Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy; (F.D.); (A.D.F.); (M.G.); (M.G.)
| | - Lucia Altucci
- Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy;
| | - Giovanni Boccia
- Department of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84081 Salerno, Italy; (G.D.); (F.D.P.); (V.I.); (G.B.)
| | - Massimiliano Galdiero
- Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy; (F.D.); (A.D.F.); (M.G.); (M.G.)
| | - Veronica Folliero
- Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy; (F.D.); (A.D.F.); (M.G.); (M.G.)
- Correspondence: (V.F.); (G.F.)
| | - Gianluigi Franci
- Department of Medicine, Surgery and Dentistry Scuola Medica Salernitana, University of Salerno, 84081 Salerno, Italy; (G.D.); (F.D.P.); (V.I.); (G.B.)
- Correspondence: (V.F.); (G.F.)
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