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Simakin AV, Baimler IV, Dikovskaya AO, Kazantseva DV, Yanykin DV, Voronov VV, Uvarov OV, Astashev ME, Sarimov RM, Ivanov VE, Bruskov VI, Kozlov VA. Laser fragmentation of amorphous and crystalline selenium of various morphologies and assessment of their antioxidant and protection properties. Front Chem 2024; 12:1459477. [PMID: 39185370 PMCID: PMC11341537 DOI: 10.3389/fchem.2024.1459477] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/04/2024] [Accepted: 07/30/2024] [Indexed: 08/27/2024] Open
Abstract
Introduction: The process of laser-induced breakdown of amorphous and crystalline selenium nanoparticles (Se NPs) of various shapes during nanosecond laser fragmentation of aqueous colloidal solutions of nanoparticles with different concentrations has been studied. Methods: The methods of studying the characteristics of plasma and acoustic oscillations induced by optical breakdown are applied. The methods of assessing the concentration of hydrogen peroxide and hydroxyl radicals, the amount of long-lived reactive species of protein and 8-oxoguanine are applied. Results: It has been established that in the process of laser fragmentation of selenium nanoparticles at a wavelength of 532 nm, corresponding to the maximum absorption of selenium, the highest probability of breakdown, the number of plasma flashes, their luminosity and the amplitude of acoustic signals are achieved at concentrations of the order of 109 NPs/mL. It has been shown that the use of selenium nanoparticles of various shapes and structures leads to a change in the photoacoustic signal during laser-induced breakdown. When crystalline selenium nanoparticles are irradiated, the intensity of the photoacoustic response during breakdown turns out to be greater (1.5 times for flash luminosity and 3 times for acoustics) than when amorphous particles are irradiated at the same concentration. It has been shown that selenium nanoparticles exhibit significant antioxidant properties. Selenium nanoparticles effectively prevent the formation of reactive oxygen species (ROS) during water radiolysis, eliminate radiation-induced long-lived reactive species of protein, and reduce the radiation-chemical yield of a key marker of oxidative DNA damage - 8-oxoguanine. Discussion: In general, the intensity of processes occurring during laser fragmentation of amorphous and crystalline selenium nanoparticles differs significantly. The antioxidant properties are more pronounced in amorphous selenium nanoparticles compared to crystalline selenium nanoparticles.
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Affiliation(s)
- Alexander V. Simakin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Ilya V. Baimler
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | | | - Dina V. Kazantseva
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Denis V. Yanykin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Valery V. Voronov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Oleg V. Uvarov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Maxim E. Astashev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Ruslan M. Sarimov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
| | - Vladimir E. Ivanov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia
| | - Vadim I. Bruskov
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia
| | - Valeriy A. Kozlov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
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Sass JO, Henke P, Mitrovic A, Weinmann M, Kluess D, Johannsen J, Sellin ML, Lembke U, Reimer D, Lork C, Jonitz-Heincke A, Bader R. Multifunctional Hybrid Material for Endoprosthetic Implants Based on Alumina-Toughened Zirconia Ceramics and Additively Manufactured TiNbTa Alloys. MATERIALS (BASEL, SWITZERLAND) 2024; 17:1838. [PMID: 38673194 PMCID: PMC11051168 DOI: 10.3390/ma17081838] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/14/2024] [Revised: 04/10/2024] [Accepted: 04/11/2024] [Indexed: 04/28/2024]
Abstract
Aseptic implant loosening after a total joint replacement is partially influenced by material-specific factors when cobalt-chromium alloys are used, including osteolysis induced by wear and corrosion products and stress shielding. Here, we aim to characterize a hybrid material consisting of alumina-toughened zirconia (ATZ) ceramics and additively manufactured Ti-35Nb-6Ta (TiNbTa) alloys, which are joined by a glass solder. The structure of the joint, the static and fatigue shear strength, the influence of accelerated aging, and the cytotoxicity with human osteoblasts are characterized. Furthermore, the biomechanical properties of the functional demonstrators of a femoral component for total knee replacements are evaluated. The TiNbTa-ATZ specimens showed a homogenous joint with statistically distributed micro-pores and a slight accumulation of Al-rich compounds at the glass solder-TiNbTa interface. Shear strengths of 26.4 ± 4.2 MPa and 38.2 ± 14.4 MPa were achieved for the TiNbTa-ATZ and Ti-ATZ specimens, respectively, and they were not significantly affected by the titanium material used, nor by accelerated aging (p = 0.07). All of the specimens survived 107 cycles of shear loading to 10 MPa. Furthermore, the TiNbTa-ATZ did not impair the proliferation and metabolic activity of the human osteoblasts. Functional demonstrators made of TiNbTa-ATZ provided a maximum bearable extension-flexion moment of 40.7 ± 2.2 Nm. The biomechanical and biological properties of TiNbTa-ATZ demonstrate potential applications for endoprosthetic implants.
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Affiliation(s)
- Jan-Oliver Sass
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
| | - Paul Henke
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
| | - Aurica Mitrovic
- ZM Praezisionsdentaltechnik GmbH, Breite Str. 16, D-18057 Rostock, Germany (C.L.)
| | | | - Daniel Kluess
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
- INNOPROFF GmbH, Joachim-Jungius-Straße 9, D-18059 Rostock, Germany
| | - Jan Johannsen
- Fraunhofer Research Institution for Additive Manufacturing Technologies IAPT, Am Schleusengraben 14, D-21029 Hamburg, Germany;
| | - Marie-Luise Sellin
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
| | - Ulrich Lembke
- DOT GmbH, Charles-Darwin-Ring 1A, D-18059 Rostock, Germany
| | - Daniel Reimer
- FMZ GmbH, Charles-Darwin-Ring 3A, D-18059 Rostock, Germany
| | - Cornelia Lork
- ZM Praezisionsdentaltechnik GmbH, Breite Str. 16, D-18057 Rostock, Germany (C.L.)
| | - Anika Jonitz-Heincke
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
| | - Rainer Bader
- Research Laboratory for Biomechanics and Implant Technology, Department of Orthopaedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock, Germany; (P.H.)
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3
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Rawat N, Benčina M, Paul D, Kovač J, Lakota K, Žigon P, Kralj-Iglič V, Ho HC, Vukomanović M, Iglič A, Junkar I. Fine-Tuning the Nanostructured Titanium Oxide Surface for Selective Biological Response. ACS APPLIED BIO MATERIALS 2023; 6:5481-5492. [PMID: 38062750 PMCID: PMC10731649 DOI: 10.1021/acsabm.3c00686] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/21/2023] [Revised: 11/07/2023] [Accepted: 11/20/2023] [Indexed: 12/19/2023]
Abstract
Cardiovascular diseases are a pre-eminent global cause of mortality in the modern world. Typically, surgical intervention with implantable medical devices such as cardiovascular stents is deployed to reinstate unobstructed blood flow. Unfortunately, existing stent materials frequently induce restenosis and thrombosis, necessitating the development of superior biomaterials. These biomaterials should inhibit platelet adhesion (mitigating stent-induced thrombosis) and smooth muscle cell proliferation (minimizing restenosis) while enhancing endothelial cell proliferation at the same time. To optimize the surface properties of Ti6Al4V medical implants, we investigated two surface treatment procedures: gaseous plasma treatment and hydrothermal treatment. We analyzed these modified surfaces through scanning electron microscopy (SEM), water contact angle analysis (WCA), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) analysis. Additionally, we assessed in vitro biological responses, including platelet adhesion and activation, as well as endothelial and smooth muscle cell proliferation. Herein, we report the influence of pre/post oxygen plasma treatment on titanium oxide layer formation via a hydrothermal technique. Our results indicate that alterations in the titanium oxide layer and surface nanotopography significantly influence cell interactions. This work offers promising insights into designing multifunctional biomaterial surfaces that selectively promote specific cell types' proliferation─which is a crucial advancement in next-generation vascular implants.
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Affiliation(s)
- Niharika Rawat
- Laboratory
of Physics, Faculty of Electrical Engineering,
University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia
| | - Metka Benčina
- Laboratory
of Physics, Faculty of Electrical Engineering,
University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia
- Department
of Surface Engineering, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
| | - Domen Paul
- Department
of Surface Engineering, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
| | - Janez Kovač
- Department
of Surface Engineering, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
| | - Katja Lakota
- Department
of Rheumatology, University Medical Centre
Ljubljana, Vodnikova 62, SI-1000 Ljubljana, Slovenia
| | - Polona Žigon
- Department
of Rheumatology, University Medical Centre
Ljubljana, Vodnikova 62, SI-1000 Ljubljana, Slovenia
| | - Veronika Kralj-Iglič
- Laboratory
of Clinical Biophysics, Faculty of Health
Sciences, University of Ljubljana, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia
| | - Hsin-Chia Ho
- Advanced
Materials Department, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
| | - Marija Vukomanović
- Advanced
Materials Department, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
| | - Aleš Iglič
- Laboratory
of Physics, Faculty of Electrical Engineering,
University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia
- Chair of
Orthopaedic Surgery, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia
| | - Ita Junkar
- Department
of Surface Engineering, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia
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Gudkov SV, Gao M, Simakin AV, Baryshev AS, Pobedonostsev RV, Baimler IV, Rebezov MB, Sarimov RM, Astashev ME, Dikovskaya AO, Molkova EA, Kozlov VA, Bunkin NF, Sevostyanov MA, Kolmakov AG, Kaplan MA, Sharapov MG, Ivanov VE, Bruskov VI, Kalinichenko VP, Aiyyzhy KO, Voronov VV, Pimpha N, Li R, Shafeev GA. Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress. MATERIALS (BASEL, SWITZERLAND) 2023; 16:5164. [PMID: 37512437 PMCID: PMC10386620 DOI: 10.3390/ma16145164] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/27/2023] [Revised: 06/25/2023] [Accepted: 07/17/2023] [Indexed: 07/30/2023]
Abstract
With the help of laser ablation, a technology for obtaining nanosized crystalline selenium particles (SeNPs) has been created. The SeNPs do not exhibit significant toxic properties, in contrast to molecular selenium compounds. The administration of SeNPs can significantly increase the viabilities of SH-SY5Y and PCMF cells after radiation exposure. The introduction of such nanoparticles into the animal body protects proteins and DNA from radiation-induced damage. The number of chromosomal breaks and oxidized proteins decreases in irradiated mice treated with SeNPs. Using hematological tests, it was found that a decrease in radiation-induced leukopenia and thrombocytopenia is observed when selenium nanoparticles are injected into mice before exposure to ionizing radiation. The administration of SeNPs to animals 5 h before radiation exposure in sublethal and lethal doses significantly increases their survival rate. The modification dose factor for animal survival was 1.2. It has been shown that the introduction of selenium nanoparticles significantly normalizes gene expression in the cells of the red bone marrow of mice after exposure to ionizing radiation. Thus, it has been demonstrated that SeNPs are a new gene-protective and radioprotective agent that can significantly reduce the harmful effects of ionizing radiation.
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Affiliation(s)
- Sergey V Gudkov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
- Russian Scientific-Research Institute of Phytopathology of Russian Academy of Sciences, 143050 Big Vyazemy, Russia
- Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 603022 Nizhny Novgorod, Russia
| | - Meng Gao
- State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou 215123, China
| | - Alexander V Simakin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Alexey S Baryshev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Roman V Pobedonostsev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Ilya V Baimler
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Maksim B Rebezov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Ruslan M Sarimov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Maxim E Astashev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
- Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center "Push-chino Scientific Center for Biological Research of the Russian Academy of Sciences", Institutskaya St., 3, 142290 Pushchino, Russia
| | - Anastasia O Dikovskaya
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Elena A Molkova
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Valery A Kozlov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
- Department of Fundamental Sciences, Bauman Moscow State Technical University, 2-nd Baumanskaya Str. 5, 105005 Moscow, Russia
| | - Nikolay F Bunkin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
- Department of Fundamental Sciences, Bauman Moscow State Technical University, 2-nd Baumanskaya Str. 5, 105005 Moscow, Russia
| | - Mikhail A Sevostyanov
- Russian Scientific-Research Institute of Phytopathology of Russian Academy of Sciences, 143050 Big Vyazemy, Russia
- A. A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Alexey G Kolmakov
- A. A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Mikhail A Kaplan
- A. A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Mars G Sharapov
- Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center "Push-chino Scientific Center for Biological Research of the Russian Academy of Sciences", Institutskaya St., 3, 142290 Pushchino, Russia
| | - Vladimir E Ivanov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Institutskaya St. 3, 142290 Pushchino, Russia
| | - Vadim I Bruskov
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Institutskaya St. 3, 142290 Pushchino, Russia
| | - Valery P Kalinichenko
- Russian Scientific-Research Institute of Phytopathology of Russian Academy of Sciences, 143050 Big Vyazemy, Russia
- Institute of Fertility of Soils of South Russia, 346493 Persianovka, Russia
| | - Kuder O Aiyyzhy
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Valery V Voronov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
| | - Nuttaporn Pimpha
- National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA) 111, Phahonyotin Rd, Klong Luang 12120, Thailand
| | - Ruibin Li
- State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou 215123, China
| | - Georgy A Shafeev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia
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Gudkov SV, Li R, Serov DA, Burmistrov DE, Baimler IV, Baryshev AS, Simakin AV, Uvarov OV, Astashev ME, Nefedova NB, Smolentsev SY, Onegov AV, Sevostyanov MA, Kolmakov AG, Kaplan MA, Drozdov A, Tolordava ER, Semenova AA, Lisitsyn AB, Lednev VN. Fluoroplast Doped by Ag 2O Nanoparticles as New Repairing Non-Cytotoxic Antibacterial Coating for Meat Industry. Int J Mol Sci 2023; 24:ijms24010869. [PMID: 36614309 PMCID: PMC9821803 DOI: 10.3390/ijms24010869] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 12/20/2022] [Accepted: 12/28/2022] [Indexed: 01/05/2023] Open
Abstract
Foodborne infections are an important global health problem due to their high prevalence and potential for severe complications. Bacterial contamination of meat during processing at the enterprise can be a source of foodborne infections. Polymeric coatings with antibacterial properties can be applied to prevent bacterial contamination. A composite coating based on fluoroplast and Ag2O NPs can serve as such a coating. In present study, we, for the first time, created a composite coating based on fluoroplast and Ag2O NPs. Using laser ablation in water, we obtained spherical Ag2O NPs with an average size of 45 nm and a ζ-potential of -32 mV. The resulting Ag2O NPs at concentrations of 0.001-0.1% were transferred into acetone and mixed with a fluoroplast-based varnish. The developed coating made it possible to completely eliminate damage to a Teflon cutting board. The fluoroplast/Ag2O NP coating was free of defects and inhomogeneities at the nano level. The fluoroplast/Ag2O NP composite increased the production of ROS (H2O2, OH radical), 8-oxogualnine in DNA in vitro, and long-lived active forms of proteins. The effect depended on the mass fraction of the added Ag2O NPs. The 0.01-0.1% fluoroplast/NP Ag2O coating exhibited excellent bacteriostatic and bactericidal properties against both Gram-positive and Gram-negative bacteria but did not affect the viability of eukaryotic cells. The developed PTFE/NP Ag2O 0.01-0.1% coating can be used to protect cutting boards from bacterial contamination in the meat processing industry.
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Affiliation(s)
- Sergey V. Gudkov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
- All-Russia Research Institute of Phytopathology of the Russian Academy of Sciences, Institute St., 5, Big Vyazyomy, 143050 Moscow, Russia
- Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 603105 Nizhny Novgorod, Russia
| | - Ruibin Li
- School for Radiologic and Interdisciplinary Science, Soochow University, Suzhou 215123, China
| | - Dmitriy A. Serov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
- Institute of Cell Biophysics, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya St., 3, 142290 Pushchino, Russia
| | - Dmitriy E. Burmistrov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
| | - Ilya V. Baimler
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
| | - Alexey S. Baryshev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
| | - Alexander V. Simakin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
| | - Oleg V. Uvarov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
| | - Maxim E. Astashev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
- Institute of Cell Biophysics, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya St., 3, 142290 Pushchino, Russia
| | - Natalia B. Nefedova
- Institute of Cell Biophysics, Russian Academy of Sciences, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya St., 3, 142290 Pushchino, Russia
- Federal State Budget Educational Institution of Higher Education Pushchino State Institute of Natural Science, Science Av. 3, 142290 Pushchino, Russia
| | | | - Andrey V. Onegov
- Mari State University, pl. Lenina, 1, 424001 Yoshkar-Ola, Russia
| | - Mikhail A. Sevostyanov
- All-Russia Research Institute of Phytopathology of the Russian Academy of Sciences, Institute St., 5, Big Vyazyomy, 143050 Moscow, Russia
- A.A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Alexey G. Kolmakov
- A.A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Mikhail A. Kaplan
- A.A. Baikov Institute of Metallurgy and Materials Science (IMET RAS) of the Russian Academy of Sciences, Leninsky Prospect, 49, 119334 Moscow, Russia
| | - Andrey Drozdov
- Institute for Analytical Instrumentation of the Russian Academy of Sciences, Ulitsa Ivana Chernykh, 31–33, lit. A, 198095 St. Petersburg, Russia
| | - Eteri R. Tolordava
- V. M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, Talalikhina St., 26, 109316 Moscow, Russia
| | - Anastasia A. Semenova
- V. M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, Talalikhina St., 26, 109316 Moscow, Russia
| | - Andrey B. Lisitsyn
- V. M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, Talalikhina St., 26, 109316 Moscow, Russia
| | - Vasily N. Lednev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia
- Correspondence:
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6
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Sotniczuk A, Jastrzębska A, Chlanda A, Kwiatek A, Garbacz H. How Streptococcus mutans Affects the Surface Topography and Electrochemical Behavior of Nanostructured Bulk Ti. Biomolecules 2022; 12:biom12101515. [PMID: 36291724 PMCID: PMC9599476 DOI: 10.3390/biom12101515] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2022] [Revised: 10/07/2022] [Accepted: 10/15/2022] [Indexed: 11/19/2022] Open
Abstract
The metabolization of carbohydrates by Streptococcus mutans leads to the formation of lactic acid in the oral cavity, which can consequently accelerate the degradation of dental implants fabricated from commercially available microcrystalline Ti. Microstructure influences surface topography and hence interaction between bacteria cells and Ti surfaces. This work offers the first description of the effect of S. mutans on the surface topography and properties of nanostructured bulk Ti, which is a promising candidate for modern narrow dental implants owing to its superior mechanical strength. It was found that S. mutans incubation resulted in the slight, unexpected decrease of surface nanoroughness, which was previously developed owing to privileged oxidation in areas of closely spaced boundaries. However, despite the changes in nanoscale surface topography, bacteria incubation did not reduce the high level of protection afforded by the oxide layer formed on the nanostructured Ti surface. The results highlight the need–hitherto ignored–to consider Ti microstructure when analyzing its behavior in the presence of carbohydrate-metabolizing bacteria.
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Affiliation(s)
- Agata Sotniczuk
- Faculty of Materials Science and Engineering, Warsaw University of Technology, 02-507 Warsaw, Poland
- Correspondence:
| | - Agnieszka Jastrzębska
- Faculty of Materials Science and Engineering, Warsaw University of Technology, 02-507 Warsaw, Poland
| | - Adrian Chlanda
- Łukasiewicz Research Network—Institute of Microelectronics and Photonics, 01-919 Warsaw, Poland
| | - Agnieszka Kwiatek
- Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland
| | - Halina Garbacz
- Faculty of Materials Science and Engineering, Warsaw University of Technology, 02-507 Warsaw, Poland
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7
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Abstract
Nanocomposites based on polymers and nanoparticles are used in agriculture for photoconversion of solar radiation, as a basis for covering material, as a packaging material, and as functional films. At the same time, nanocomposites are almost never used in agriculture as biosafe structural materials. In this work, we have developed a technology for obtaining a nanocomposite based on PLGA and iron oxide nanoparticles. The nanocomposite has unique physical and chemical properties and also exhibits pronounced antibacterial properties at a concentration of iron oxide nanoparticles of more than 0.01%. At the same time, the nanocomposite does not affect the growth and development of pepper and is biocompatible with mammalian cells. Nanocomposites based on PLGA and iron oxide nanoparticles can be an attractive candidate for the manufacture of structural and packaging materials in agriculture.
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8
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Trueba P, Navarro C, Giner M, Rodríguez-Ortiz JA, Montoya-García MJ, Delgado-Pujol EJ, Rodríguez-Albelo LM, Torres Y. Approach to the Fatigue and Cellular Behavior of Superficially Modified Porous Titanium Dental Implants. MATERIALS 2022; 15:ma15113903. [PMID: 35683200 PMCID: PMC9182243 DOI: 10.3390/ma15113903] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/11/2022] [Revised: 05/25/2022] [Accepted: 05/27/2022] [Indexed: 12/04/2022]
Abstract
In this work, the fatigue and cellular performance of novel superficially treated porous titanium dental implants made up using conventional powder metallurgy and space-holder techniques (30 vol.% and 50 vol.%, both with a spacer size range of 100–200 µm) are evaluated. Before the sintering stage, a specific stage of CNC milling of the screw thread of the implant is used. After the consolidation processing, different surface modifications are performed: chemical etching and bioactive coatings (BG 45S5 and BG 1393). The results are discussed in terms of the effect of the porosity, as well as the surface roughness, chemical composition, and adherence of the coatings on the fatigue resistance and the osteoblast cells’ behavior for the proposed implants. Macro-pores are preferential sites of the nucleation of cracks and bone cell adhesion, and they increase the cellular activity of the implants, but decrease the fatigue life. In conclusion, SH 30 vol.% dental implant chemical etching presents the best bio-functional (in vitro osseointegration) and bio-mechanical (stiffness, yield strength and fatigue life) balance, which could ensure the required characteristics of cortical bone tissue.
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Affiliation(s)
- Paloma Trueba
- Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Seville, Spain; (P.T.); (J.A.R.-O.); (E.J.D.-P.); (L.M.R.-A.); (Y.T.)
| | - Carlos Navarro
- Departamento de Ingeniería Mecánica y Fabricación, Escuela Técnica Superior de Ingeniería, Universidad de Sevilla, 41092 Seville, Spain;
| | - Mercè Giner
- Departamento de Citología e Histología Normal y Patológica, Universidad de Sevilla, 41009 Sevilla, Spain
- Correspondence: ; Tel.: +34-(9)-54551796
| | - José A. Rodríguez-Ortiz
- Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Seville, Spain; (P.T.); (J.A.R.-O.); (E.J.D.-P.); (L.M.R.-A.); (Y.T.)
| | | | - Ernesto J. Delgado-Pujol
- Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Seville, Spain; (P.T.); (J.A.R.-O.); (E.J.D.-P.); (L.M.R.-A.); (Y.T.)
| | - Luisa M. Rodríguez-Albelo
- Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Seville, Spain; (P.T.); (J.A.R.-O.); (E.J.D.-P.); (L.M.R.-A.); (Y.T.)
| | - Yadir Torres
- Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Seville, Spain; (P.T.); (J.A.R.-O.); (E.J.D.-P.); (L.M.R.-A.); (Y.T.)
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9
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Addressing the Needs of the Rapidly Aging Society through the Development of Multifunctional Bioactive Coatings for Orthopedic Applications. Int J Mol Sci 2022; 23:ijms23052786. [PMID: 35269928 PMCID: PMC8911303 DOI: 10.3390/ijms23052786] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2022] [Revised: 02/27/2022] [Accepted: 02/28/2022] [Indexed: 12/15/2022] Open
Abstract
The unprecedented aging of the world's population will boost the need for orthopedic implants and expose their current limitations to a greater extent due to the medical complexity of elderly patients and longer indwelling times of the implanted materials. Biocompatible metals with multifunctional bioactive coatings promise to provide the means for the controlled and tailorable release of different medications for patient-specific treatment while prolonging the material's lifespan and thus improving the surgical outcome. The objective of this work is to provide a review of several groups of biocompatible materials that might be utilized as constituents for the development of multifunctional bioactive coatings on metal materials with a focus on antimicrobial, pain-relieving, and anticoagulant properties. Moreover, the review presents a summary of medications used in clinical settings, the disadvantages of the commercially available products, and insight into the latest development strategies. For a more successful translation of such research into clinical practice, extensive knowledge of the chemical interactions between the components and a detailed understanding of the properties and mechanisms of biological matter are required. Moreover, the cost-efficiency of the surface treatment should be considered in the development process.
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10
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Antibacterial behavior of organosilicon composite with nano aluminum oxide without influencing animal cells. REACT FUNCT POLYM 2022. [DOI: 10.1016/j.reactfunctpolym.2021.105143] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
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11
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Burmistrov DE, Simakin AV, Smirnova VV, Uvarov OV, Ivashkin PI, Kucherov RN, Ivanov VE, Bruskov VI, Sevostyanov MA, Baikin AS, Kozlov VA, Rebezov MB, Semenova AA, Lisitsyn AB, Vedunova MV, Gudkov SV. Bacteriostatic and Cytotoxic Properties of Composite Material Based on ZnO Nanoparticles in PLGA Obtained by Low Temperature Method. Polymers (Basel) 2021; 14:49. [PMID: 35012071 PMCID: PMC8747160 DOI: 10.3390/polym14010049] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/28/2021] [Revised: 12/13/2021] [Accepted: 12/20/2021] [Indexed: 12/13/2022] Open
Abstract
A low-temperature technology was developed for producing a nanocomposite based on poly (lactic-co-glycolic acid) and zinc oxide nanoparticles (ZnO-NPs), synthesized by laser ablation. Nanocomposites were created containing 0.001, 0.01, and 0.1% of zinc oxide nanoparticles with rod-like morphology and a size of 40-70 nm. The surface of the films from the obtained nanomaterial was uniform, without significant defects. Clustering of ZnO-NPs in the PLGA matrix was noted, which increased with an increase in the concentration of the dopant in the polymer. The resulting nanomaterial was capable of generating reactive oxygen species (ROS), such as hydrogen peroxide and hydroxyl radicals. The rate of ROS generation increased with an increase in the concentration of the dopant. It was shown that the synthesized nanocomposite promotes the formation of long-lived reactive protein species, and is also the reason for the appearance of a key biomarker of oxidative stress, 8-oxoguanine, in DNA. The intensity of the process increased with an increase in the concentration of nanoparticles in the matrix. It was found that the nanocomposite exhibits significant bacteriostatic properties, the severity of which depends on the concentration of nanoparticles. In particular, on the surface of the PLGA-ZnO-NPs composite film containing 0.001% nanoparticles, the number of bacterial cells was 50% lower than that of pure PLGA. The surface of the composite is non-toxic to eukaryotic cells and does not interfere with their adhesion, growth, and division. Due to its low cytotoxicity and bacteriostatic properties, this nanocomposite can be used as coatings for packaging in the food industry, additives for textiles, and also as a material for biomedicine.
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Affiliation(s)
- Dmitriy E. Burmistrov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
| | - Alexander V. Simakin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
| | - Veronika V. Smirnova
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
| | - Oleg V. Uvarov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
| | - Petr I. Ivashkin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
| | - Roman N. Kucherov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
- Moscow Engineering Physics Institute, National Research Nuclear University MEPhI, Kashirskoe Highway 31, 115409 Moscow, Russia
| | - Vladimir E. Ivanov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 3 Institutskaya St., 142290 Pushchino, Russia;
| | - Vadim I. Bruskov
- Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 3 Institutskaya St., 142290 Pushchino, Russia;
| | - Mihail A. Sevostyanov
- A. A. Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Leninsky Prospect 49, 119991 Moscow, Russia; (M.A.S.); (A.S.B.)
| | - Alexander S. Baikin
- A. A. Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Leninsky Prospect 49, 119991 Moscow, Russia; (M.A.S.); (A.S.B.)
| | - Valery A. Kozlov
- Faculty of Fundamental Sciences, Bauman Moscow State Technical University, Vtoraya Baumanskaya Ul. 5, 105005 Moscow, Russia;
| | - Maksim B. Rebezov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
- V. M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, 109316 Moscow, Russia; (A.A.S.); (A.B.L.)
| | - Anastasia A. Semenova
- V. M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, 109316 Moscow, Russia; (A.A.S.); (A.B.L.)
| | - Andrey B. Lisitsyn
- V. M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, 109316 Moscow, Russia; (A.A.S.); (A.B.L.)
| | - Maria V. Vedunova
- Institute of Biology and Biomedicine, Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, 603950 Nizhny Novgorod, Russia;
| | - Sergey V. Gudkov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (A.V.S.); (V.V.S.); (O.V.U.); (P.I.I.); (R.N.K.); (V.E.I.); (M.B.R.)
- Institute of Biology and Biomedicine, Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, 603950 Nizhny Novgorod, Russia;
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12
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Burmistrov DE, Yanykin DV, Paskhin MO, Nagaev EV, Efimov AD, Kaziev AV, Ageychenkov DG, Gudkov SV. Additive Production of a Material Based on an Acrylic Polymer with a Nanoscale Layer of Zno Nanorods Deposited Using a Direct Current Magnetron Discharge: Morphology, Photoconversion Properties, and Biosafety. MATERIALS 2021; 14:ma14216586. [PMID: 34772111 PMCID: PMC8585381 DOI: 10.3390/ma14216586] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 10/16/2021] [Revised: 10/30/2021] [Accepted: 11/01/2021] [Indexed: 01/31/2023]
Abstract
On the basis of a direct current magnetron, a technology has been developed for producing nanoscale-oriented nanorods from zinc oxide on an acrylic polymer. The technology makes it possible to achieve different filling of the surface with zinc oxide nanorods. The nanorods is partially fused into the polymer; the cross section of the nanorods is rather close to an elongated ellipse. It is shown that, with intense abrasion, no delamination of the nanorods from the acrylic polymer is observed. The zinc oxide nanorods abrades together with the acrylic polymer. Zinc oxide nanorods luminesces with the wavelength most preferable for the process of photosynthesis in higher plants. It was shown that plants grown under the obtained material grow faster and gain biomass faster than the control group. In addition, it was found that on surfaces containing zinc oxide nanorods, a more intense formation of such reactive oxygen species as hydrogen peroxide and hydroxyl radical is observed. Intensive formation of long-lived, active forms of the protein is observed on the zinc oxide coating. The formation of 8-oxoguanine in DNA in vitro on a zinc oxide coating was shown using ELISA method. It was found that the multiplication of microorganisms on the developed material is significantly hampered. At the same time, eukaryotic cells of animals grow and develop without hindrance. Thus, the material we have obtained can be used in photonics (photoconversion material for greenhouses, housings for LEDs), and it is also an affordable and non-toxic nanomaterial for creating antibacterial coatings.
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Affiliation(s)
- Dmitry E. Burmistrov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
| | - Denis V. Yanykin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
| | - Mark O. Paskhin
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
| | - Egor V. Nagaev
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
| | - Alexey D. Efimov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
| | - Andrey V. Kaziev
- Moscow Engineering Physics Institute, National Research Nuclear University MEPhI, Kashirskoe Sh. 31, 115409 Moscow, Russia; (A.V.K.); (D.G.A.)
| | - Dmitry G. Ageychenkov
- Moscow Engineering Physics Institute, National Research Nuclear University MEPhI, Kashirskoe Sh. 31, 115409 Moscow, Russia; (A.V.K.); (D.G.A.)
| | - Sergey V. Gudkov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia; (D.E.B.); (D.V.Y.); (M.O.P.); (E.V.N.); (A.D.E.)
- Correspondence:
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13
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Gudkov SV, Simakin AV, Sarimov RM, Kurilov AD, Chausov DN. Novel Biocompatible with Animal Cells Composite Material Based on Organosilicon Polymers and Fullerenes with Light-Induced Bacteriostatic Properties. NANOMATERIALS (BASEL, SWITZERLAND) 2021; 11:2804. [PMID: 34835569 PMCID: PMC8625234 DOI: 10.3390/nano11112804] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/17/2021] [Revised: 10/15/2021] [Accepted: 10/21/2021] [Indexed: 11/17/2022]
Abstract
A technology for producing a nanocomposite based on the borsiloxane polymer and chemically unmodified fullerenes has been developed. Nanocomposites containing 0.001, 0.01, and 0.1 wt% fullerene molecules have been created. It has been shown that the nanocomposite with any content of fullerene molecules did not lose the main rheological properties of borsiloxane and is capable of structural self-healing. The resulting nanomaterial is capable of generating reactive oxygen species (ROS) such as hydrogen peroxide and hydroxyl radicals in light. The rate of ROS generation increases with an increase in the concentration of fullerene molecules. In the absence of light, the nanocomposite exhibits antioxidant properties. The severity of antioxidant properties is also associated with the concentration of fullerene molecules in the polymer. It has been shown that the nanocomposite upon exposure to visible light leads to the formation of long-lived reactive protein species, and is also the reason for the appearance of such a key biomarker of oxidative stress as 8-oxoguanine in DNA. The intensity of the process increases with an increase in the concentration of fullerene molecules. In the dark, the polymer exhibits weak protective properties. It was found that under the action of light, the nanocomposite exhibits significant bacteriostatic properties, and the severity of these properties depends on the concentration of fullerene molecules. Moreover, it was found that bacterial cells adhere to the surfaces of the nanocomposite, and the nanocomposite can detach bacterial cells not only from the surfaces, but also from wetted substrates. The ability to capture bacterial cells is primarily associated with the properties of the polymer; they are weakly affected by both visible light and fullerene molecules. The nanocomposite is non-toxic to eukaryotic cells, the surface of the nanocomposite is suitable for eukaryotic cells for colonization. Due to the combination of self-healing properties, low cytotoxicity, and the presence of bacteriostatic properties, the nanocomposite can be used as a reusable dry disinfectant, as well as a material used in prosthetics.
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Affiliation(s)
- Sergey V. Gudkov
- Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilova St., 38, 119991 Moscow, Russia; (A.V.S.); (R.M.S.); (A.D.K.); (D.N.C.)
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Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System. NANOMATERIALS 2020; 10:nano10122531. [PMID: 33339399 PMCID: PMC7767124 DOI: 10.3390/nano10122531] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/09/2020] [Revised: 12/11/2020] [Accepted: 12/11/2020] [Indexed: 02/08/2023]
Abstract
Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15x(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs.
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New Nanostructured Carbon Coating Inhibits Bacterial Growth, but Does Not Influence on Animal Cells. NANOMATERIALS 2020; 10:nano10112130. [PMID: 33120890 PMCID: PMC7692575 DOI: 10.3390/nano10112130] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 10/02/2020] [Revised: 10/16/2020] [Accepted: 10/20/2020] [Indexed: 12/11/2022]
Abstract
An electrospark technology has been developed for obtaining a colloidal solution containing nanosized amorphous carbon. The advantages of the technology are its low cost and high performance. The colloidal solution of nanosized carbon is highly stable. The coatings on its basis are nanostructured. They are characterized by high adhesion and hydrophobicity. It was found that the propagation of microorganisms on nanosized carbon coatings is significantly hindered. At the same time, eukaryotic animal cells grow and develop on nanosized carbon coatings, as well as on the nitinol medical alloy. The use of a colloidal solution as available, cheap and non-toxic nanomaterial for the creation of antibacterial coatings to prevent biofilm formation seems to be very promising for modern medicine, pharmaceutical and food industries.
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