Review
Copyright ©2014 Baishideng Publishing Group Co.
World J Clin Oncol. May 10, 2014; 5(2): 114-124
Published online May 10, 2014. doi: 10.5306/wjco.v5.i2.114
Table 1 Detection of circulating and disseminated tumour cells in oral squamous cell carcinoma and head neck squamous cell cancer
SourceEntitynAssay systemCTC-positive (%)DTC-positive (%)Clinical relevanceNumber of analysed OSCCRef. yr of release
PBOSCC20RT-PCR: CK19 mRNA20.0Detection of CK19-mRNA when tumour is incisedOSCC only[85], 2000
PBHNSCC77RT-PCR: CK19 mRNA2.1No significance for CTCsVarious sites n = 13 OSCC n = 23 oropharyngeal[79], 2001
PB/ CVB BMHNSCC40ICC: Keratins CK1-8, 10, 14-16 and 19 RT-PCR: E48 mRNA10.0 (PCR) 20.0 (ICC)20.0 (PCR) 32.0 (ICC)E48 mRNA and DMFS/DFS preoperative in BM (P = 0.001/P = 0.002) and CVB (P < 0.001/P = 0.013) E48-mRNA and DMFS/DFS intraoperative in CVB (P = 0.026/0.001) ICC and DMFS preoperative in BM (P = 0.0006) and CVB (P < 0.001) ICC and DFS preoperative in BM (P = 0.036) and CVB (P = 0.042)OSCC only[81], 2003
BMHNSCC162RT-PCR: E48 mRNA40.0DTC on DFS (P = 0.1460) DMFS (P = 0.2912) N > or = 2 DMFS (P = 0.0210)Various sites n = 13 OSCC n = 23 oropharyngeal[80], 2004
BMHNSCC176ICC: CK1930.7OS and N-involvement (P = 0.0001), OS and DTC (0.0066)Various sites n = 37 OSCC n = 43 oropharyngeal[29], 2004
PBHNSCC21Immunomagnetic separation: EpCAM33.3Occurrence of CTCs more frequent in stage III-IV than in stageI-II; connection with recurrence presumed; NSVarious sites n = 7 OSCC/ oropharyngeal[83], 2007
PBOSCC25RT-PCR: CK19 mRNA16Detection of CK19-mRNA when tumour is incisedOSCC only[108], 2008
PBHNSCC48Immunomagnetic separation: ICC: CK3-6H571DFS and NO CTC (P = 0.01) clinical outcome and CTCs (P = 0.04)Various sites n = 25 OSCC n = 10 oropharyngeal[30], 2010
PBHNSCC42Flow cytometry: EpCAM, CD45 RT-PCR: EGFR mRNA43CTC and N in flow cytometry (P = 0.014), CTC and N in RT-PCRVarious sites n = 9 OSCC n = 17 oropharyngeal[31], 2011
PBHNSCC15CellSearch™40CTC and M (P = 0.04)Various sites n = 4 OSCC n =7 oropharyngeal[84], 2012
PBHNSCC31Flow cytometry: EpCAM; EGFR, CD4529 pEGFR in 55% of CTC+-casesIncreasing number of CTC-positive cases at end of treatmentVarious sites n =5 OSCC n =18 oropharyngeal[50], 2012
PBUnder RCT HNSCC33Flow cytometry: EpCAM CD45 keratins 7, 8 EGFR33 pEGFR in 36.4% of CTC+-casesNone given for comparing two methodsVarious sites n = 0 OSCC (not specified) n = 13 oropharyngeal[51], 2012
PBHNSCC73CellSearch™15.1Decreasing CTC counts and clinical response (P = 0.017)Various sites n = 3 OSCC n = 39 oropharyngeal[82], 2012
BMHNSCC105ICC: keratins 8, 18 and 1914.5No significance for DTCs (76/105 tested)Various sites n = 72 OSCC n = 8 oropharyngeal[68], 2012
PB BMOSCC110CellSearch™ ICC: keratins 8, 18 and 1912.5 (Cell search)20.0 (ICC)CTCs and T (P = 0.04), N and DTCs (P = 0.02), M and CTCs (P = 0.004), M and DTCs (P = 0.005), DTCs and RFS (P < 0.001)OSCC only[35], 2014