Original Article
Copyright ©2014 Baishideng Publishing Group Co.
World J Cardiol. Apr 26, 2014; 6(4): 183-195
Published online Apr 26, 2014. doi: 10.4330/wjc.v6.i4.183
Figure 1
Figure 1 Generation of B19V-genotype 1 to 3 specific restriction fragment length polymorphism-polymerase chain reaction. A: Schematic representation of the B19V genome showing localization of the B19V-genotype-specific restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) in the B19V NS1-VP1u region. Sequences of B19V-1, B19V-2 and B19V-3 showing the RFLP-PCR fragment and HpaI and TaqI restriction enzyme sites (lower panel). Primer positions for 1st and 2nd RFLP-PCR are indicated (1. PCR and 2. PCR, see also Table 2); B: Expected fragment size and digestion pattern after HpaI and TaqI digestions (right panel). Agarose gel electrophoresis showing respective PCR-fragments after HpaI and TaqI digestion for each B19V-genotype (left panel); C: Representative agarose gel electrophoresis of patient-specific B19V RFLP-PCRs. B19V-1: B19V-genotype 1.