• Rh antibody
• Rh antigen
• Rh phenotype
• compatible blood transfusion

• alloimmunization
• blood donors
• blood group antigens
• phenotype

• alloimmunization
• indirect antihuman globulin test
• pregnant women

• funding

• compatibility tests
• local red blood cell panel
• performance
• unexpected antibodies

• Child
• Humans
• Female
• Pregnancy
• Child, Preschool
• Indicators and Reagents
• Burkina Faso
• Feasibility Studies
• Antibodies
• Erythrocytes

• Addis Ababa
• Ethiopia
• RH
• Rh-D sensitization
• alloimmunization
• associated factors
• prevalence

Erythrocyte alloantibodies are mainly produced after immune stimulation, such as blood transfusion, pregnancy, and transplantation, and are the leading causes of severe hemolytic transfusion reactions and difficulty in blood grouping and matching. Therefore, antibody screening is critical to prevent and improve red cell alloantibodies. Routine tube assay is the primary detection method of antibody screening. Recently, erythrocyte-magnetized technology (EMT) has been increasingly used in clinical practice. This study intends to probe the application and efficacy of the conventional tube and EMT in red blood cell alloantibody titration to provide a reference for clinical blood transfusion.

To investigate the application value of conventional tube and EMT in red blood cell alloantibody titration and enhance the safety of blood transfusion practice.

A total of 1298 blood samples were harvested from blood donors at the Department of Blood Transfusion of our hospital from March 2021 to December 2022. A 5 mL blood sample was collected in tubing, which was then cut, and the whole blood was put into a test tube for centrifugation to separate the serum. Different red blood cell blood group antibody titers were simultaneously detected using the tube polybrene test, tube antiglobulin test (AGT), and EMT screening irregular antibody methods to determine the best test method.

Simultaneous detection was performed through the tube polybrene test, tube AGT and EMT screening irregular antibodies. It was discovered that the EMT screening irregular antibody method could detect all immunoglobulin G (IgG) and immunoglobulin M (IgM) irregular antibodies, and the results of manual tube AGT were satisfactory, but the operation time was lengthy, and the equipment had a large footprint. The EMT screening irregular antibody assay was also conducted to determine its activity against type O Rh (D) red blood cells, and the outcomes were satisfactory. Furthermore, compared to the conventional tube method, the EMT screening irregular antibody method was more cost-effective and had significantly higher detection efficiency.

With a higher detection rate, the EMT screening irregular antibody method can detect both IgG and IgM irregular antibodies faster and more effectively than the conventional tube method.

• Erythrocyte-magnetized technology
• Conventional tube
• Red blood cell alloantibodies
• Transfusion reactions
• Application

• alloimmunization
• blood transfusion
• irregular antibodies
• paediatrics
• red blood cells

• alloantibodies
• antibodies
• red blood cells
• transfusion

In Burkina Faso, red blood cell (RBC) transfusion remains the crucial anaemia treatment following chronic renal failure (CRF) as erythropoietin and its analogues are unavailable. However, blood group matching beyond the ABO and Rhesus is not common in Burkina Faso. Thus, alloimmunisation is a potential issue for transfused patients.

Our study aimed to identify anti-erythrocyte antibodies in multi-transfused CRF patients at the Yalgado Ouedraogo Teaching Hospital, Ouagadougou, Burkina Faso.

This cross-sectional study, conducted from October 2018 to November 2019, included CRF patients who had received at least two RBC units. We screened patients for the presence of RBC antibodies using three commercial Cells panels and identified antibody specificities for positive screenings using 11 Cells panels for an indirect antiglobulin test (IAT) in a low ionic strength microcolumn gel-card system.

Two hundred and thirty-five patients (45.1% female; average age: 41.5 years) were included. The median number of blood units received per patient was 10 (interquartile range: 5–20). The overall alloimmunisation rate was 5.9% (14/235). Antibodies identified included: anti-D (1 case), anti-C (1 case), anti-D+C (4 cases), anti-C^W (1 case), anti-E (1 case), anti-S (1 case) and anti-Le^a (1 case). In four positive patients, the specificity of the antibodies was indeterminate. No risk factors were associated with alloimmunisation.

In Burkina Faso, screening for RBC alloantibodies should be mandated for patients at risk. The high rate of indeterminate antibodies suggests the need to develop a local RBC antibody panel adapted to the local population.

• Hydroxyurea
• Sickle cell anemia
• Stroke
• Sub-Saharan Africa
• Transcranial Doppler

• Child
• Humans
• Female
• Child, Preschool
• Adolescent
• Male
• Hydroxyurea/adverse effects
• Prospective Studies
• Splenomegaly/complications
• Stroke/etiology
• Stroke/prevention & control
• Stroke/epidemiology
• Anemia, Sickle Cell/complications
• Anemia, Sickle Cell/drug therapy
• Africa South of the Sahara

• K23 HL153763 NHLBI NIH HHS

• alloimmunization
• antibodies
• extended phenotyping
• red blood cells
• transfusion

• Rh-ee
• alloimmunization
• anti-E
• blood transfusion

• alloantibodies
• cryoprotectant
• diagnostics
• freeze-drying
• reagent red blood cells
• transfusion

• Alloimmunity
• Antibody
• Asian people
• Erythrocyte
• Gender
• Japanese
• Patients
• Pregnancy
• Transfusion

• Alloimmunization
• Child
• Neonate
• Red blood cell
• Transfusion

• Alloimmunization
• Sickle cell disease
• Sub-Saharan Africa
• Systematic review

• Allosensitization
• Antigens
• Blood groups
• Blood transfusion

• blood banking
• gel testing
• hemolytic transfusion reaction
• pretransfusion testing
• transfusion medicine
• tube testing

The realization of red cell exchange (RCE) in Africa faces the lack of blood, transfusion safety, and equipment. We evaluated its efficacy and safety in severe complications of sickle cell disease.

Manual partial RCE was performed among sickle cell patients who had severe complications. Efficacy was evaluated by clinical evolution, blood count, and electrophoresis of hemoglobin. Safety was evaluated on adverse effects, infections, and alloimmunization.

We performed 166 partial RCE among 44 patients including 41 homozygous (SS) and 2 heterozygous composites SC and 1 S/β0-thalassemia. The mean age was 27.9 years. The sex ratio was 1.58. The regression of symptoms was complete in 100% of persistent vasoocclusive crisis and acute chest syndrome, 56.7% of intermittent priapism, and 30% of stroke. It was partial in 100% of leg ulcers and null in acute priapism. The mean variations of hemoglobin and hematocrit rate after one procedure were, respectively, +1.4 g/dL and +4.4%. That of hemoglobin S after 2 consecutive RCE was −60%. Neither alloimmunization nor viral seroconversion was observed.

This work shows the feasibility of manual partial RCE in a low-resource setting and its efficacy and safety during complications of SCD outside of acute priapism.

• Red blood cell (RBC)
• delayed cord clamping (DCC)
• exchange transfusions (ET)