Identification of key genes controlling cancer stem cell characteristics in gastric cancer

Figure 1 Differential analysis of mRNA expression-based stemness index and screening of differentially expressed genes. A: Expression of mRNA expression-based stemness index in gastric tumor tissues and normal gastric tissues; B: Significantly differentially expressed genes in gastric tumor tissues and normal gastric tissues.

Figure 2 Weighted gene coexpression network analysis. A: Clustering dendrograms of differentially expressed genes. Each piece of the leaves on the cluster dendrogram corresponds to a gene. The first color band indicates the modules detected by dynamic tree cut methods. The second color band indicates the modules after merging similar modules; B: Correlation between the gene module and mRNA expression-based stemness index (mRNAsi). The correlation coefficient in each cell represents the correlation between the gene module and mRNAsi, which increases in size from blue to red. The corresponding P value is also annotated; C: Scatter plot of module eigengenes in brown. The top right corner is where the key genes are located.

Figure 3 Analysis of key genes. A: Expression of the key genes in gastric tumor tissues and normal gastric tissues. The red histogram indicates gastric tumor tissue. The blue histogram indicates normal gastric tissue. ^cP < 0.001; B: Correlation of key genes. The number in the box represents the size of the correlation, and the larger the number, the higher the correlation; C: Protein-protein interaction networks of key genes. The color of the lines represents the source of the evidence; D: Connection nodes of key genes. The number represents the number of connection nodes.

Figure 4 Significantly enriched gene ontology biological process terms. The larger the circle, the more number of enrichment, and the redder the color, the more significant the difference. BP: Biological process; CC: Cell component; MF: Molecular function.

Figure 5 Survival curves of key genes using the online tool Kaplan-Meier Plotter. The red indicates high expression and black indicates low expression. A log-rank P < 0.05 was considered statistically significant.

Figure 6 Expression of key genes in gastric cancer based on the Oncomine online database. The key genes were highly expressed in gastric cancer tissues.