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Krzesiak A, Lavoie JL, Sebille S, Cognard C, Bosquet L, Delpech N. Post-exercise hypotension in male spontaneously hypertensive rats: The issue of calculation method. Physiol Rep 2023; 11:e15524. [PMID: 36807709 PMCID: PMC9937782 DOI: 10.14814/phy2.15524] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/08/2022] [Revised: 10/30/2022] [Accepted: 11/07/2022] [Indexed: 02/19/2023] Open
Abstract
In spontaneously hypertensive rats, exercise can lead to a post-exercise decrease in blood pressure, named post-exercise hypotension (PEH). This can be following physical training but also after a single bout of mild to moderate exercise when measured with tail-cuff or externalized catheter methods. Our aim was to assess the PEH obtained with different calculation methods and to compare the magnitude of this effect induced by a moderate-intensity continuous exercise or a high-intensity intermittent exercise. Thirteen 16-week-old male spontaneously hypertensive rats performed two types of aerobic exercise (continuous or intermittent) on a treadmill. Arterial pressure was recorded by telemetry for 24 h which was started 3 h before physical exercise. Based on the literature, PEH was first evaluated with two different baseline values, and then with three different approaches. We observed that the identification of PEH depended on the method used to measure the rest value, and that its amplitude was also influenced by the calculation approach and the type of exercise performed. Hence, the calculation method and the amplitude of the detected PEH can significantly influence their physiological and pathophysiological inferences.
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Affiliation(s)
- Amandine Krzesiak
- Laboratoire MObilité, Vieillissement et Exercice; EA 6314, Faculté des Sciences du SportPoitiersFrance
- Laboratoire Signalisation & Transports Ioniques MembranairesERL CNRS/Université de Poitiers no 7368PoitiersFrance
| | - Julie L. Lavoie
- Centre de recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM)MontréalCanada
- École de kinésiologie et des sciences de l'activité physiqueUniversité de MontréalMontréalCanada
| | - Stéphane Sebille
- Laboratoire Signalisation & Transports Ioniques MembranairesERL CNRS/Université de Poitiers no 7368PoitiersFrance
| | - Christian Cognard
- Laboratoire Signalisation & Transports Ioniques MembranairesERL CNRS/Université de Poitiers no 7368PoitiersFrance
| | - Laurent Bosquet
- Laboratoire MObilité, Vieillissement et Exercice; EA 6314, Faculté des Sciences du SportPoitiersFrance
- École de kinésiologie et des sciences de l'activité physiqueUniversité de MontréalMontréalCanada
| | - Nathalie Delpech
- Laboratoire MObilité, Vieillissement et Exercice; EA 6314, Faculté des Sciences du SportPoitiersFrance
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Araujo JES, Santos RMD, Oliveira DPM, Macedo FN, Quintans JSS, Barreto RSS, Santos SL, Santos MRV, Junior LJQ, Barreto AS. Resistance training increases insulin-induced vasodilation in the mesenteric artery of healthy rats. AN ACAD BRAS CIENC 2021; 93:e20210222. [PMID: 34909827 DOI: 10.1590/0001-3765202120210222] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/19/2021] [Accepted: 09/07/2021] [Indexed: 11/22/2022] Open
Abstract
This study evaluated the ability of resistance training (RT) of moderate intensity to promote vascular changes in insulin-induced vasodilation in healthy animals. Wistar rats were divided into two groups: control (CON) and trained (eight weeks of training, performing 3 sets with 10 repetitions at 60% of maximum intensity). Forty-eight hours after the last session of the RT, the animals were sacrificed and vascular reactivity to insulin in the absence and presence of LY294002 (phosphatidylinositol 3-kinase inhibitors (PI3K), L-NAME (nitric oxide synthase (NOS) inhibitors) and BQ123 (endothelin A antagonist (ET-A) receptor). In addition, phenylephrine (Phe)-induced vasoconstriction in the absence and presence of L-NAME was also evaluated. The RT group showed greater vasodilation in maximal response compared to the CON group. After PI3K inhibition, vasodilation was reduced in both groups. However, when the NOS participation was evaluated, the RT group showed contraction in relation to the CON group, which was abolished by BQ123. In addition, the RT group had an increase in nitrite levels compared to the CON group. When the Phe response was evaluated, there was a reduction in tension in the RT group compared to the CON group. The results suggest that RT improves vascular reactivity.
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Affiliation(s)
- João E S Araujo
- Universidade Tiradentes, Departamento de Educação Física, Rua José Paulo Santana, 1254, 49500-000 Itabaiana, SE, Brazil.,Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil
| | - Rodrigo M Dos Santos
- Universidade Federal de Sergipe, Laboratório de Biologia Cardiovascular e Estresse Oxidativo, Departamento de Fisiologia, Av. Marechal Rondon, s/n, Rosa Elze, 49100-100 São Cristovão, SE, Brazil
| | - Davi P M Oliveira
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil
| | - Fabrício N Macedo
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Centro Universitário Estácio de Sergipe, Rua Teixeira de Freitas, 10, Salgado Filho, 49020-490 Aracajú, SE, Brazil
| | - Jullyana S S Quintans
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Programa de Pós-Graduação em Ciências da Saúde, Rua Cláudio Batista, s/n, Cidade Nova, 49060-108 Aracajú, SE, Brazil
| | - Rosana S S Barreto
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Programa de Pós-Graduação em Ciências da Saúde, Rua Cláudio Batista, s/n, Cidade Nova, 49060-108 Aracajú, SE, Brazil
| | - Sandra L Santos
- Universidade Federal de Sergipe, Laboratório de Biologia Cardiovascular e Estresse Oxidativo, Departamento de Fisiologia, Av. Marechal Rondon, s/n, Rosa Elze, 49100-100 São Cristovão, SE, Brazil
| | - Marcio R V Santos
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Programa de Pós-Graduação em Ciências da Saúde, Rua Cláudio Batista, s/n, Cidade Nova, 49060-108 Aracajú, SE, Brazil
| | - Lucindo J Q Junior
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Programa de Pós-Graduação em Ciências da Saúde, Rua Cláudio Batista, s/n, Cidade Nova, 49060-108 Aracajú, SE, Brazil
| | - André S Barreto
- Universidade Federal de Sergipe, Laboratório de Farmacologia Cardiovascular, Departamento de Fisiologia, Rosa Elze, Av. Marechal Rondon, s/n, 49100-100, São Cristovão, SE, Brazil.,Programa de Pós-Graduação em Ciências da Saúde, Rua Cláudio Batista, s/n, Cidade Nova, 49060-108 Aracajú, SE, Brazil
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Facioli TDP, Buranello MC, Regueiro EMG, Basso-Vanelli RP, Durand MDT. Effect of Physical Training on Nitric Oxide Levels in Patients with Arterial Hypertension: An Integrative Review. INTERNATIONAL JOURNAL OF CARDIOVASCULAR SCIENCES 2021. [DOI: 10.36660/ijcs.20200244] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/23/2023] Open
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Linear periodization of strength training in blocks attenuates hypertension and diastolic dysfunction with normalization of myocardial collagen content in spontaneously hypertensive rats. J Hypertens 2020; 38:73-81. [PMID: 31335510 DOI: 10.1097/hjh.0000000000002188] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/24/2022]
Abstract
BACKGROUND AND METHOD This study evaluated the effects of a linear block strength training programme on the parameters of cardiac remodelling in spontaneously hypertensive rats. Thirty-nine rats were equally distributed in four groups: normotensive sedentary, normotensive trained, hypertensive sedentary and hypertensive trained. The strength training protocol was organized in three mesocycles of 4 weeks, with an increase in the training load organized in a linear fashion for each block, considering the weight established in the maximum loaded load test. The following parameters were evaluated: ventricular function assessed by echocardiogram, caudal blood pressure, ventricular haemodynamics and cardiac masses. Two-way analysis of variance was used to determine the differences between the group and time. RESULTS After 12 weeks of training, the hypertensive trained group presented the following results: increased muscle strength, reduced blood pressure, reduced heart rate, isovolumetric relaxation time and total collagen content, with increased cardiac function, without promoting changes in the mass and nuclear volume of cardiomyocytes. Also, blood pressure reduction seems to be associated with both muscle strength adjustments and total load progress. CONCLUSION The findings of this study showed that the training programme carried out attenuated systemic arterial pressure and preserved the ventricular function of spontaneously hypertensive rats without cardiac mass change.
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ARAUJO JOÃOE, MACEDO FABRÍCION, OLIVEIRA DAVIP, BRITTO RAQUELM, QUINTANS JULLYANAS, BARRETO ROSANAS, SANTOS MARCIOR, QUINTANS-JUNIOR LUCINDOJ, BARRETO ANDRÉS. Resistance training prevents the reduction of insulin-mediated vasodilation in the mesenteric artery of dexamethasone-treated rats. AN ACAD BRAS CIENC 2020; 92:e20200316. [DOI: 10.1590/0001-3765202020200316] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/05/2020] [Accepted: 07/22/2020] [Indexed: 12/30/2022] Open
Affiliation(s)
- JOÃO E.S. ARAUJO
- Universidade Federal de Sergipe, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Brazil; Universidade Tiradentes, Brazil
| | | | | | | | - JULLYANA S.S. QUINTANS
- Universidade Federal de Sergipe, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Brazil
| | - ROSANA S.S. BARRETO
- Universidade Federal de Sergipe, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Brazil
| | - MARCIO R.V. SANTOS
- Universidade Federal de Sergipe, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Brazil
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Melo S, da Silva Júnior N, Barauna V, Oliveira E. Cardiovascular Adaptations Induced by Resistance Training in Animal Models. Int J Med Sci 2018; 15:403-410. [PMID: 29511376 PMCID: PMC5835711 DOI: 10.7150/ijms.23150] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/03/2017] [Accepted: 12/05/2017] [Indexed: 02/06/2023] Open
Abstract
In the last 10 years the number of studies showing the benefits of resistance training (RT) to the cardiovascular system, have grown. In comparison to aerobic training, RT-induced favorable adaptations to the cardiovascular system have been ignored for many years, thus the mechanisms of the RT-induced cardiovascular adaptations are still uncovered. The lack of animal models with comparable protocols to the RT performed by humans hampers the knowledge. We have used squat-exercise model, which is widely used by many others laboratories. However, to a lesser extent, other models are also employed to investigate the cardiovascular adaptations. In the subsequent sections we will review the information regarding cardiac morphological adaptations, signaling pathway of the cardiac cell, cardiac function and the vascular adaptation induced by RT using this animal model developed by Tamaki et al. in 1992. Furthermore, we also describe cardiovascular findings observed using other animal models of RT.
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Affiliation(s)
- S.F.S. Melo
- Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo. Address: Av. Marechal Campos, 1468 Maruípe, Espírito Santo, Vitória, Brazil. Postal code: 29043900. Telephone number: (5527)996892407
- Laboratory of Biochemistry and Molecular Biology of Exercise, School of Physical Education and Sport, University of São Paulo. Address: Av. Prof. Mello Moraes, 65, Cidade Universitária, São Paulo, São Paulo, Brazil. Postal code: 05508-9000. Telephone number: (5511) 30913136
| | - N.D. da Silva Júnior
- Laboratory of Biochemistry and Molecular Biology of Exercise, School of Physical Education and Sport, University of São Paulo. Address: Av. Prof. Mello Moraes, 65, Cidade Universitária, São Paulo, São Paulo, Brazil. Postal code: 05508-9000. Telephone number: (5511) 30913136
| | - V.G. Barauna
- Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo. Address: Av. Marechal Campos, 1468 Maruípe, Espírito Santo, Vitória, Brazil. Postal code: 29043900. Telephone number: (5527)996892407
| | - E.M. Oliveira
- Laboratory of Biochemistry and Molecular Biology of Exercise, School of Physical Education and Sport, University of São Paulo. Address: Av. Prof. Mello Moraes, 65, Cidade Universitária, São Paulo, São Paulo, Brazil. Postal code: 05508-9000. Telephone number: (5511) 30913136
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Ribeiro F, Ribeiro IP, Gonçalves AC, Alves AJ, Melo E, Fernandes R, Costa R, Sarmento-Ribeiro AB, Duarte JA, Carreira IM, Witkowski S, Oliveira J. Effects of resistance exercise on endothelial progenitor cell mobilization in women. Sci Rep 2017; 7:17880. [PMID: 29259281 PMCID: PMC5736626 DOI: 10.1038/s41598-017-18156-6] [Citation(s) in RCA: 44] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2017] [Accepted: 11/28/2017] [Indexed: 12/11/2022] Open
Abstract
This study aimed to determine the effect of a single bout of resistance exercise at different intensities on the mobilization of circulating EPCs over 24 hours in women. In addition, the angiogenic factors stromal cell-derived factor 1 (SDF-1α), vascular endothelial growth factor (VEGF), hypoxia-inducible factor 1-alpha (HIF-1α) and erythropoietin (EPO) were measured as potential mechanisms for exercise-induced EPCs mobilization. Thirty-eight women performed a resistance exercise session at an intensity of 60% (n = 13), 70% (n = 12) or 80% (n = 13) of one repetition maximum. Each session was comprised of three sets of 12 repetitions of four exercises: bench press, dumbbell curl, dumbbell squat, and standing dumbbell upright row. Blood was sampled at baseline and immediately, 6 hours, and 24 hours post-exercise. Circulating EPC and levels of VEGF, HIF-1α and EPO were significantly higher after exercise (P < 0.05). The change in EPCs from baseline was greatest in the 80% group (P < 0.05), reaching the highest at 6 hours post-exercise. The change in EPCs from baseline to 6 hours post-exercise was correlated with the change in VEGF (r = 0.492, P = 0.002) and HIF-1α (r = 0.388, P = 0.016). In general, a dose-response relationship was observed, with the highest exercise intensities promoting the highest increases in EPCs and angiogenic factors.
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Affiliation(s)
- Fernando Ribeiro
- School of Health Sciences and Institute of Biomedicine - iBiMED, University of Aveiro, Aveiro, Portugal.
| | - Ilda P Ribeiro
- Center of Investigation on Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, and Center for Neuroscience and Cell Biology and Institute for Biomedical Imaging and Life Sciences (CNC.IBILI), Coimbra, Portugal
| | - Ana C Gonçalves
- Center of Investigation on Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, and Center for Neuroscience and Cell Biology and Institute for Biomedical Imaging and Life Sciences (CNC.IBILI), Coimbra, Portugal.,Laboratory of Oncobiology and Hematology, University Clinic of Hematology and Applied Molecular Biology, Faculty of Medicine, and Clinical Hematology Department, Centro Hospitalar Universitário de Coimbra (CHUC), Coimbra, Portugal
| | - Alberto J Alves
- Research Centre in Sports Sciences, Health and Human Development, CIDESD, University Institute of Maia, ISMAI, Maia, Portugal
| | - Elsa Melo
- School of Health Sciences, University of Aveiro, Aveiro, Portugal
| | - Raquel Fernandes
- School of Health Sciences and Institute of Biomedicine - iBiMED, University of Aveiro, Aveiro, Portugal
| | - Rui Costa
- School of Health Sciences and CINTESIS.UA, University of Aveiro, Aveiro, Portugal
| | - Ana B Sarmento-Ribeiro
- Center of Investigation on Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, and Center for Neuroscience and Cell Biology and Institute for Biomedical Imaging and Life Sciences (CNC.IBILI), Coimbra, Portugal.,Laboratory of Oncobiology and Hematology, University Clinic of Hematology and Applied Molecular Biology, Faculty of Medicine, and Clinical Hematology Department, Centro Hospitalar Universitário de Coimbra (CHUC), Coimbra, Portugal.,Hematology Department, Centro Hospitalar Universitário de Coimbra, Coimbra, Portugal
| | - José A Duarte
- Research Center in Physical Activity, Health and Leisure, CIAFEL, Faculty of Sport, University of Porto, Porto, Portugal
| | - Isabel M Carreira
- Center of Investigation on Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, and Center for Neuroscience and Cell Biology and Institute for Biomedical Imaging and Life Sciences (CNC.IBILI), Coimbra, Portugal
| | - Sarah Witkowski
- Department of Kinesiology, University of Massachusetts Amherst, Amherst, Massachusetts, United States of America
| | - José Oliveira
- Research Center in Physical Activity, Health and Leisure, CIAFEL, Faculty of Sport, University of Porto, Porto, Portugal
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Mota MM, Silva TLTBD, Macedo FN, Mesquita TRR, Quintans LJ, Santana-Filho VJD, Lauton-Santos S, Santos MRV. Effects of a Single Bout of Resistance Exercise in Different Volumes on Endothelium Adaptations in Healthy Animals. Arq Bras Cardiol 2017; 108:436-442. [PMID: 28591321 PMCID: PMC5444890 DOI: 10.5935/abc.20170060] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2016] [Accepted: 12/19/2016] [Indexed: 12/14/2022] Open
Abstract
Background: Resistance exercise (RE) has been recommended for patients with cardiovascular diseases. Recently, a few studies have demonstrated that the intensity of a single bout of RE has an effect on endothelial adaptations to exercise. However, there is no data about the effects of different volumes of RE on endothelium function. Objective: The aim of the study was to evaluate the effects of different volumes of RE in a single bout on endothelium-dependent vasodilatation and nitric oxide (NO) synthesis in the mesenteric artery of healthy animals. Methods: Male Wistar rats were divided into three groups: Control (Ct); low-volume RE (LV, 5 sets x 10 repetitions) and high-volume RE (HV, 15 sets x 10 repetitions). The established intensity was 70% of the maximal repetition test. After the exercise protocol, rings of mesenteric artery were used for assessment of vascular reactivity, and other mesenteric arteries were prepared for detection of measure NO production by DAF-FM fluorescence. Insulin responsiveness on NO synthesis was evaluated by stimulating the vascular rings with insulin (10 nM). Results: The maximal relaxation response to insulin increased in the HV group only as compared with the Ct group. Moreover, the inhibition of nitric oxide synthesis (L-NAME) completely abolished the insulin-induced vasorelaxation in exercised rats. NO production showed a volume-dependent increase in the endothelial and smooth muscle layer. In endothelial layer, only Ct and LV groups showed a significant increase in NO synthesis when compared to their respective group under basal condition. On the other hand, in smooth muscle layer, NO fluorescence increased in all groups when compared to their respective group under basal condition. Conclusions: Our results suggest that a single bout of RE promotes vascular endothelium changes in a volume-dependent manner. The 15 sets x 10 repetitions exercise plan induced the greatest levels of NO synthesis. Fundamentos: O exercício resistido (ER) tem sido recomendado para pacientes com doenças cardiovasculares. Recentemente, alguns estudos demonstraram que a intensidade de uma sessão de ER exerce um efeito sobre a disfunção endotelial. No entanto, não há dados sobre os efeitos de diferentes volumes de ER sobre a função endotelial. Objetivo: O objetivo deste estudo foi avaliar os efeitos de diferentes volumes de ER, realizados em uma única sessão, sobre a vasodilatação dependente do endotélio e síntese de óxido nítrico (NO) em artéria mesentérica de animais saudáveis. Métodos: Ratos Wistar machos foram divididos em três grupos: Controle (Ct); baixo volume (BV, 5 séries x 10 repetições) e alto volume de ER (AV, 15 séries x 10 repetições). Foi estabelecida a intensidade de 70% do teste de repetição máxima. Após o protocolo de exercício, anéis de artéria mesentérica foram utilizados na avaliação da reatividade vascular, e outras artérias mesentéricas foram preparadas para a detecção da produção de NO por fluorescência com para do DAF-FM. A resposta à insulina pela síntese de NO foi avaliada estimulando-se os anéis vasculares com insulina (10nM). Resultados: A resposta máxima do relaxamento induzido por insulina foi aumentada somente no grupo AV em comparação ao grupo Ct. Além disso, a inibição da síntese do NO (L-NAME), aboliu completamente o relaxamento vascular induzido por insulina em ratos exercitados. A produção de NO mostrou um aumento dependente do volume no endotélio e no músculo liso. No endotélio, apenas os grupos Ct e BV mostraram aumento significativo na síntese de NO quando comparado aos seus respectivos grupos sob condição basal. No entanto, no músculo liso, a fluorescência foi aumentada em todos os grupos quando comparados aos seus respectivos grupos sob a condição basal. Conclusões: Nossos resultados sugerem que uma única sessão de ER foi capaz de promover adaptações no endotélio vascular. Além disso, nós observamos que este efeito é volume-dependente e o volume de 15 séries x10 repetições induziu o maior aumento na síntese de NO.
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Affiliation(s)
- Marcelo Mendonça Mota
- Universidade Federal de Sergipe, Aracaju, SE - Brazil.,Curso de Educação Física da Faculdade Estácio de Sergipe (Estácio/FASE), Aracaju, SE - Brazil
| | | | - Fabricio Nunes Macedo
- Universidade Federal de Sergipe, Aracaju, SE - Brazil.,Curso de Educação Física da Faculdade Estácio de Sergipe (Estácio/FASE), Aracaju, SE - Brazil
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Faria TDO, Angeli JK, Mello LGM, Pinto GC, Stefanon I, Vassallo DV, Lizardo JHDF. A Single Resistance Exercise Session Improves Aortic Endothelial Function in Hypertensive Rats. Arq Bras Cardiol 2017; 108:228-236. [PMID: 28443955 PMCID: PMC5389872 DOI: 10.5935/abc.20170023] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/13/2016] [Accepted: 09/27/2016] [Indexed: 01/22/2023] Open
Abstract
BACKGROUND Physical exercise is an important tool for the improvement of endothelial function. OBJECTIVE To assess the effects of acute dynamic resistance exercise on the endothelial function of spontaneously hypertensive rats (SHR). METHODS Ten minutes after exercise, the aorta was removed to evaluate the expression of endothelial nitric oxide synthase (eNOS), phosphorylated endothelial nitric oxide synthase (p-eNOS1177) and inducible nitric oxide synthase (iNOS) and to generate concentration-response curves to acetylcholine (ACh) and to phenylephrine (PHE). The PHE protocol was also performed with damaged endothelium and before and after NG-nitro-L-arginine methyl ester (L-NAME) and indomethacin administration. The maximal response (Emax) and the sensitivity (EC50) to these drugs were evaluated. RESULTS ACh-induced relaxation increased in the aortic rings of exercised (Ex) rats (Emax= -80 ± 4.6%, p < 0.05) when compared to those of controls (Ct) (Emax = -50 ± 6.8%). The Emax to PHE was decreased following exercise conditions (95 ± 7.9%, p < 0.05) when compared to control conditions (120 ± 4.2%). This response was abolished after L-NAME administration or endothelial damage. In the presence of indomethacin, the aortic rings' reactivity to PHE was decreased in both groups (EC50= Ex -5.9 ± 0.14 vs. Ct -6.6 ± 0.33 log µM, p < 0.05 / Emax = Ex 9.5 ± 2.9 vs. Ct 17 ± 6.2%, p < 0.05). Exercise did not alter the expression of eNOS and iNOS, but increased the level of p-eNOS. CONCLUSION A single resistance exercise session improves endothelial function in hypertensive rats. This response seems to be mediated by increased NO production through eNOS activation.
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Ashtary-Larky D, Lamuchi-Deli N, Milajerdi A, Bakhtiar Salehi M, Alipour M, Kooti W, Ashtary-Larky P, Alamiri F, Sheikhi A, Afrisham R. Inflammatory and Biochemical Biomarkers in Response to High Intensity Resistance Training in Trained and Untrained Men. Asian J Sports Med 2017; In Press. [DOI: 10.5812/asjsm.13739] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 05/17/2025] Open
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Physical Exercise Is a Potential "Medicine" for Atherosclerosis. ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY 2017; 999:269-286. [PMID: 29022268 DOI: 10.1007/978-981-10-4307-9_15] [Citation(s) in RCA: 25] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Cardiovascular disease (CVD) has been recognized as the number one killer for decades. The most well-known risk factor is atherosclerosis. Unlike the acuity of CVD, atherosclerosis is a chronic, progressive pathological change. This process involves inflammatory response, oxidative reaction, macrophage activity, and different interaction of inflammatory factors. Physical exercise has long been known as good for health in general. In recent studies, physical exercise has been demonstrated to be a therapeutic tool for atherosclerosis. However, its therapeutic effect has dosage-dependent effect. Un-proper over exercise might also cause damage to the heart. Here we summarize the mechanism of Physical exercise's beneficial effects and its potential clinical use.
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12
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Short-term high salt intake reduces brachial artery and microvascular function in the absence of changes in blood pressure. J Hypertens 2016; 34:676-84. [PMID: 26848993 DOI: 10.1097/hjh.0000000000000852] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/11/2023]
Abstract
OBJECTIVES The aims of this study were to test the hypothesis that short-term high salt intake reduces macrovascular and microvascular endothelial function in the absence of changes in blood pressure and to determine whether acute exercise restores endothelial function after high salt in women. MATERIALS AND METHODS Twelve women were administered high salt (11 g of sodium chloride for 7 days) and then underwent a weightlifting session. Brachial artery flow-mediated dilation and nitroglycerin dilation were measured with ultrasound at baseline, after high salt, and after weightlifting. Subcutaneous fat tissue biopsies were obtained at baseline, after high salt, and after weightlifting. Resistance arteries from biopsies were cannulated for vascular reactivity measurements in response to flow [flow-induced dilation (FID)] and acetylcholine. RESULTS Blood pressure was similar before and after high salt diet. Brachial flow-mediated dilation was reduced after high salt diet but was not affected by acute weightlifting. Brachial nitroglycerin dilations were similar before and after high salt. FID and acetylcholine-induced dilation of resistance arteries were similar to that of before and after high salt diet. FID and acetylcholine-induced dilation was not altered by weightlifting after high salt diet. However, N-nitro-L-arginine methyl ester significantly reduced FID at baseline and after exercise but had no effect dilator reactivity after high salt diet alone. CONCLUSION These data suggest that high salt intake reduces brachial artery endothelial function and switches the mediator of vasodilation in the microcirculation to a non-nitric oxide-dependent mechanism in healthy adults and acute exercise may switch the dilator mechanism back to nitric oxide during high salt diet.
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Neves RVP, Souza MK, Passos CS, Bacurau RFP, Simoes HG, Prestes J, Boim MA, Câmara NOS, Franco MDCP, Moraes MR. Resistance Training in Spontaneously Hypertensive Rats with Severe Hypertension. Arq Bras Cardiol 2016; 106:201-9. [PMID: 26840054 PMCID: PMC4811275 DOI: 10.5935/abc.20160019] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/05/2015] [Accepted: 11/13/2015] [Indexed: 11/20/2022] Open
Abstract
BACKGROUND Resistance training (RT) has been recommended as a non-pharmacological treatment for moderate hypertension. In spite of the important role of exercise intensity on training prescription, there is still no data regarding the effects of RT intensity on severe hypertension (SH). OBJECTIVE This study examined the effects of two RT protocols (vertical ladder climbing), performed at different overloads of maximal weight carried (MWC), on blood pressure (BP) and muscle strength of spontaneously hypertensive rats (SHR) with SH. METHODS Fifteen male SHR ENT#091;206 ± 10 mmHg of systolic BP (SBP)ENT#093; and five Wistar Kyoto rats (WKY; 119 ± 10 mmHg of SBP) were divided into 4 groups: sedentary (SED-WKY) and SHR (SED-SHR); RT1-SHR training relative to body weight (~40% of MWC); and RT2-SHR training relative to MWC test (~70% of MWC). Systolic BP and heart rate (HR) were measured weekly using the tail-cuff method. The progression of muscle strength was determined once every fifteen days. The RT consisted of 3 weekly sessions on non-consecutive days for 12-weeks. RESULTS Both RT protocols prevented the increase in SBP (delta - 5 and -7 mmHg, respectively; p > 0.05), whereas SBP of the SED-SHR group increased by 19 mmHg (p < 0.05). There was a decrease in HR only for the RT1 group (p < 0.05). There was a higher increase in strength in the RT2 (140%; p < 0.05) group as compared with RT1 (11%; p > 0.05). CONCLUSIONS Our data indicated that both RT protocols were effective in preventing chronic elevation of SBP in SH. Additionally, a higher RT overload induced a greater increase in muscle strength.
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Affiliation(s)
| | - Michel Kendy Souza
- Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brazil
| | - Clévia Santos Passos
- Divisão de Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
| | | | - Herbert Gustavo Simoes
- Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brazil
| | - Jonato Prestes
- Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brazil
| | - Mirian Aparecida Boim
- Divisão de Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
| | - Niels Olsen Saraiva Câmara
- Laboratório de Imunologia Clínica e Experimental, Divisão de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil
| | - Maria do Carmo Pinho Franco
- Programa de Pós-Graduação em Medicina Translacional, Universidade Federal de São Paulo, São Paulo, SP, Brazil
| | - Milton Rocha Moraes
- Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brazil
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Speretta GF, Silva AA, Vendramini RC, Zanesco A, Delbin MA, Menani JV, Bassi M, Colombari E, Colombari DSA. Resistance training prevents the cardiovascular changes caused by high-fat diet. Life Sci 2016; 146:154-62. [PMID: 26776833 DOI: 10.1016/j.lfs.2016.01.011] [Citation(s) in RCA: 38] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/05/2015] [Revised: 12/04/2015] [Accepted: 01/07/2016] [Indexed: 12/31/2022]
Abstract
AIMS Aerobic exercise is indicated for prevention and treatment of obesity-induced cardiovascular disorders. Although the resistance training (RT) may also produce effects similar to aerobic exercise, this is not completely clear yet. In the present study, we tested if RT in moderate intensity might prevent alterations in blood pressure (BP), sympathetic modulation of systolic blood pressure (SBP), baroreflex function and the changes in renin-angiotensin system (RAS) and cytokines mRNA expression within the nucleus of the tract solitary (NTS) in rats fed with high-fat diet (HFD). MAIN METHODS Male Holtzman rats (300-320 g) were divided into 4 groups: sedentary with standard chow diet (SED-SD); sedentary with high-fat diet (SED-HFD); RT with standard chow diet (RT-SD); and RT with high-fat diet (RT-HFD). The trained groups performed a total of 10 weeks of moderate intensity RT in a vertical ladder. In the first 3 weeks all experimental groups were fed with SD. In the next 7 weeks, the SED-HFD and RT-HFD groups were fed with HFD. KEY FINDINGS In SED-HFD, BP and sympathetic modulation of SBP increased, whereas baroreflex bradycardic responses were attenuated. RT prevented the cardiovascular and inflammatory responses (increases in tumoral necrosis factor-α and interleukin-1β) produced by HFD in SED rats. The anti-inflammatory interleukin-10, angiotensin type 2 receptor, Mas receptor and angiotensin converting enzyme 2 mRNA expressions in the NTS increased in the RT-HFD compared to SED-HFD. SIGNIFICANCE The data demonstrated that moderate intensity RT prevented obesity-induced cardiovascular disorders simultaneously with reduced inflammatory responses and modifications of RAS in the NTS.
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Affiliation(s)
- Guilherme F Speretta
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil
| | - André A Silva
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil
| | - Regina C Vendramini
- Department of Clinical Analysis, School of Pharmaceutical Sciences, UNESP, Araraquara, SP, Brazil
| | - Angelina Zanesco
- Department of Physical Education, Institute of Bioscience, UNESP, Rio Claro, SP, Brazil
| | - Maria A Delbin
- Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil
| | - José V Menani
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil
| | - Mirian Bassi
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil
| | - Eduardo Colombari
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil
| | - Débora S A Colombari
- Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
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15
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Mota MM, Mesquita TRR, Silva TLTBD, Fontes MT, Lauton Santos S, Capettini LDSA, Jesus ICGD, Quintans Júnior LJ, De Angelis K, Wichi RB, Santos MRV. Endothelium adjustments to acute resistance exercise are intensity-dependent in healthy animals. Life Sci 2015; 142:86-91. [DOI: 10.1016/j.lfs.2015.10.007] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/26/2015] [Revised: 09/15/2015] [Accepted: 10/06/2015] [Indexed: 10/22/2022]
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da Silva TLTB, Mota MM, Fontes MT, Araújo JEDS, Carvalho VO, Bonjardim LR, Santos MRV. Effects of one resistance exercise session on vascular smooth muscle of hypertensive rats. Arq Bras Cardiol 2015; 105:160-7. [PMID: 26107814 PMCID: PMC4559125 DOI: 10.5935/abc.20150070] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/16/2014] [Revised: 03/12/2015] [Accepted: 03/23/2015] [Indexed: 12/31/2022] Open
Abstract
BACKGROUND Hypertension is a public health problem and increases the incidence of cardiovascular diseases. OBJECTIVE To evaluate the effects of a resistance exercise session on the contractile and relaxing mechanisms of vascular smooth muscle in mesenteric arteries of NG-nitro L-arginine methyl ester (L-NAME)-induced hypertensive rats. METHODS Wistar rats were divided into three groups: control (C), hypertensive (H), and exercised hypertensive (EH). Hypertension was induced by administration of 20 mg/kg of L-NAME for 7 days prior to experimental protocols. The resistance exercise protocol consisted of 10 sets of 10 repetitions and intensity of 40% of one repetition maximum. The reactivity of vascular smooth muscle was evaluated by concentration‑response curves to phenylephrine (PHEN), potassium chloride (KCl) and sodium nitroprusside (SNP). RESULTS Rats treated with L-NAME showed an increase (p < 0.001) in systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) compared to the initial period of induction. No difference in PHEN sensitivity was observed between groups H and EH. Acute resistance exercise reduced (p < 0.001) the contractile response induced by KCl at concentrations of 40 and 60 mM in group EH. Greater (p < 0.01) smooth muscle sensitivity to NPS was observed in group EH as compared to group H. CONCLUSION One resistance exercise session reduces the contractile response induced by KCl in addition to increasing the sensitivity of smooth muscle to NO in mesenteric arteries of hypertensive rats.
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17
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Fernandes AA, Faria TDO, Ribeiro Júnior RF, Costa GP, Marchezini B, Silveira EA, Angeli JK, Stefanon I, Vassallo DV, Lizardo JH. A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats. ACTA ACUST UNITED AC 2015; 48:813-21. [PMID: 26176315 PMCID: PMC4568809 DOI: 10.1590/1414-431x20154355] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/13/2014] [Accepted: 03/20/2015] [Indexed: 01/03/2023]
Abstract
Resistance training evokes myocardial adaptation; however, the effects of a single
resistance exercise session on cardiac performance are poorly understood or
investigated. This study aimed to investigate the effects of a single resistance
exercise session on the myocardial contractility of spontaneously hypertensive rats
(SHRs). Male 3-month-old SHRs were divided into two groups: control (Ct) and exercise
(Ex). Control animals were submitted to sham exercise. Blood pressure was measured in
conscious rats before the exercise session to confirm the presence of arterial
hypertension. Ten minutes after the exercise session, the animals were anesthetized
and killed, and the hearts were removed. Cardiac contractility was evaluated in the
whole heart by the Langendorff technique and by isometric contractions of isolated
left ventricular papillary muscles. SERCA2a, phospholamban (PLB), and phosphorylated
PLB expression were investigated by Western blot. Exercise increased force
development of isolated papillary muscles (Ex=1.0±0.1 g/mg vs
Ct=0.63±0.2 g/mg, P<0.05). Post-rest contraction was greater in the exercised
animals (Ex=4.1±0.4% vs Ct=1.7±0.2%, P<0.05). Papillary muscles
of exercised animals developed greater force under increasing isoproterenol
concentrations (P<0.05). In the isolated heart, exercise increased left
ventricular isovolumetric systolic pressure (LVISP; Δ +39 mmHg; P<0.05) from
baseline conditions. Hearts from the exercised rats presented a greater response to
increasing diastolic pressure. Positive inotropic intervention to calcium and
isoproterenol resulted in greater LVISP in exercised animals (P<0.05). The results
demonstrated that a single resistance exercise session improved myocardial
contractility in SHRs.
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Affiliation(s)
- A A Fernandes
- Departamento de Morfologia, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - T de O Faria
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - R F Ribeiro Júnior
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - G P Costa
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - B Marchezini
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - E A Silveira
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - J K Angeli
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - I Stefanon
- Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, BR
| | - D V Vassallo
- Departamento de Ciências Fisiológicas, Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória, Vitória, ES, BR
| | - J H Lizardo
- Departamento de Morfologia, Universidade Federal do Espírito Santo, Vitória, ES, BR
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18
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Petriz BA, Almeida JA, Gomes CPC, Pereira RW, Murad AM, Franco OL. NanoUPLC/MS(E) proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training. J Proteomics 2014; 113:351-65. [PMID: 25451014 DOI: 10.1016/j.jprot.2014.10.010] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2014] [Revised: 10/03/2014] [Accepted: 10/17/2014] [Indexed: 10/24/2022]
Abstract
UNLABELLED NanoUPLC/MS(E) was used to verify the effects of 8weeks of low (SHR-LIT=4) and high (SHR-HIT=4) intensity training over the left ventricle proteome of hypertensive rats (SHR-C=4). Training enhanced the aerobic capacity and reduced the systolic blood pressure in all exercised rats. NanoUPLC/MS(E) identified 250 proteins, with 233 in common to all groups and 16 exclusive to SHR-C, 2 to SHR-LIT, and 2 to the SHR-HIT. Cardiac hypertrophy related proteins appeared only in SHR-C. The SHR-LIT enhanced the abundance of 30 proteins and diminished 6, while SHR-HIT enhanced the abundance of 39 proteins and reduced other 7. The levels of metabolic (β and γ-enolase, adenine phosphoribosultransferase, and cytochrome b-c1), myofibril (myosin light chain 4, tropomyosin α and β-chain), and transporter proteins (hemoglobin, serum albumin, and hemopexin) were increased by both intensities. Transcription regulator and histone variants were enhanced by SHR-LIT and SHR-HIT respectively. SHR-LIT reduced the concentration of myosin binding protein C, while desmin and membrane voltage dependent anion selective channel protein-3 were reduced only by SHR-HIT. In addition, polyubiquitin B and C, and transcription regulators decreased in both intensities. Exercise also increased the concentration of anti-oxidant proteins, peroxiredozin-6 and glutathione peroxidase-1. BIOLOGICAL SIGNIFICANCE Pathologic left ventricle hypertrophy if one of the major outcomes of hypertension being a strong predictor of heart failure. Among the various risk factors for cardiovascular disorders, arterial hypertension is responsible for the highest rates of mortality worldwide. In this way, this present study contribute to the understanding of the molecular mechanisms involved in the attenuation of hypertension and the regression of pathological cardiac hypertrophy induced by exercise training.
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Affiliation(s)
- Bernardo A Petriz
- Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; UDF - Centro Universitário, Brasília, DF, Brazil
| | - Jeeser A Almeida
- Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; UDF - Centro Universitário, Brasília, DF, Brazil; Programa de Pós Graduação em Ciências e Tecnologias em Saúde, Universidade de Brasília - UnB, Ceilândia-DF, Brazil
| | - Clarissa P C Gomes
- Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil
| | - Rinaldo W Pereira
- Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília-DF, Brazil
| | - André M Murad
- Embrapa Recursos Genéticos e Biotecnologia - Laboratório de Biologia Sintética, Brasília-DF, Brazil
| | - Octavio L Franco
- Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; S-Inova, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande MS, Brazil; Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília-DF, Brazil.
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Asano RY, Sales MM, Browne RAV, Moraes JFVN, Coelho Júnior HJ, Moraes MR, Simões HG. Acute effects of physical exercise in type 2 diabetes: A review. World J Diabetes 2014; 5:659-665. [PMID: 25317243 PMCID: PMC4138589 DOI: 10.4239/wjd.v5.i5.659] [Citation(s) in RCA: 60] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/10/2014] [Revised: 07/09/2014] [Accepted: 07/29/2014] [Indexed: 02/05/2023] Open
Abstract
The literature has shown the efficiency of exercise in the control of type 2 diabetes (T2D), being suggested as one of the best kinds of non-pharmacological treatments for its population. Thus, the scientific production related to this phenomenon has growing exponentially. However, despite its advances, still there is a lack of studies that have carried out a review on the acute effects of physical exercise on metabolic and hemodynamic markers and possible control mechanisms of these indicators in individuals with T2D, not to mention that in a related way, these themes have been very little studied today. Therefore, the aim of this study was to organize and analyze the current scientific production about the acute effects of physical exercise on metabolic and hemodynamic markers and possible control mechanisms of these indicators in T2D individuals. For such, a research with the following keywords was performed: -exercise; diabetes and post-exercise hypotension; diabetes and excess post-exercise oxygen consumption; diabetes and acute effects in PUBMED, SCIELO and HIGHWIRE databases. From the analyzed studies, it is possible to conclude that, a single exercise session can promote an increase in the bioavailability of nitric oxide and elicit decreases in postexercise blood pressure. Furthermore, the metabolic stress from physical exercise can increase the oxidation of carbohydrate during the exercise and keep it, in high levels, the post exercise consumption of O², this phenomenon increases the rate of fat oxidation during recovery periods after exercise, improves glucose tolerance and insulin sensitivity and reduces glycemia between 2-72 h, which seems to be dependent on the exercise intensity and duration of the effort.
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20
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Mota MM, da Silva TLTB, Fontes MT, Barreto AS, Araújo JEDS, de Oliveira ACC, Wichi RB, Santos MRV. Resistance exercise restores endothelial function and reduces blood pressure in type 1 diabetic rats. Arq Bras Cardiol 2014; 103:25-32. [PMID: 25120082 PMCID: PMC4126758 DOI: 10.5935/abc.20140087] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/29/2013] [Revised: 11/19/2013] [Accepted: 11/28/2013] [Indexed: 12/12/2022] Open
Abstract
BACKGROUND Resistance exercise effects on cardiovascular parameters are not consistent. OBJECTIVES The effects of resistance exercise on changes in blood glucose, blood pressure and vascular reactivity were evaluated in diabetic rats. METHODS Wistar rats were divided into three groups: control group (n = 8); sedentary diabetic (n = 8); and trained diabetic (n = 8). Resistance exercise was carried out in a squat device for rats and consisted of three sets of ten repetitions with an intensity of 50%, three times per week, for eight weeks. Changes in vascular reactivity were evaluated in superior mesenteric artery rings. RESULTS A significant reduction in the maximum response of acetylcholine-induced relaxation was observed in the sedentary diabetic group (78.1 ± 2%) and an increase in the trained diabetic group (95 ± 3%) without changing potency. In the presence of NG-nitro-L-arginine methyl ester, the acetylcholine-induced relaxation was significantly reduced in the control and trained diabetic groups, but not in the sedentary diabetic group. Furthermore, a significant increase (p < 0.05) in mean arterial blood pressure was observed in the sedentary diabetic group (104.9 ± 5 to 126.7 ± 5 mmHg) as compared to that in the control group. However, the trained diabetic group showed a significant decrease (p < 0.05) in the mean arterial blood pressure levels (126.7 ± 5 to 105.1 ± 4 mmHg) as compared to the sedentary diabetic group. CONCLUSIONS Resistance exercise could restore endothelial function and prevent an increase in arterial blood pressure in type 1 diabetic rats.
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Affiliation(s)
- Marcelo Mendonça Mota
- Departamento de Fisiologia - Universidade Federal de Sergipe (UFS), São
Cristóvão, SE − Brazil
| | | | - Milene Tavares Fontes
- Departamento de Fisiologia - Universidade Federal de Sergipe (UFS), São
Cristóvão, SE − Brazil
| | - André Sales Barreto
- Departamento de Fisiologia - Universidade Federal de Sergipe (UFS), São
Cristóvão, SE − Brazil
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21
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Gavi MBRO, Vassalo DV, Amaral FT, Macedo DCF, Gava PL, Dantas EM, Valim V. Strengthening exercises improve symptoms and quality of life but do not change autonomic modulation in fibromyalgia: a randomized clinical trial. PLoS One 2014; 9:e90767. [PMID: 24651512 PMCID: PMC3961245 DOI: 10.1371/journal.pone.0090767] [Citation(s) in RCA: 72] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/11/2013] [Accepted: 02/01/2014] [Indexed: 12/03/2022] Open
Abstract
Objective Autonomic dysfunction is an important mechanism that could explain many symptoms observed in fibromyalgia (FM). Exercise is an effective treatment, with benefits potentially mediated through changes in autonomic modulation. Strengthening is one of the less studied exercises in FM, and the acute and chronic effects of strengthening on the autonomic system remain unknown. The objective of this study was to assess the chronic effects of strengthening exercises (STRE) on autonomic modulation, pain perception and the quality of life (QOL) of FM patients. Methods Eighty sedentary women with FM (ACR 1990) were randomly selected to participate in STRE or flexibility (FLEX) exercises in a blinded controlled trial. The intensity of STRE was set at 45% of the estimated load of 1 Repetition Maximum (RM) in 12 different exercises. Outcomes were Visual Analog Scale (VAS) for pain, Heart Rate Variability (HRV) analysis, treadmill test, the sit and reach test (Wells and Dillon’s Bench), maximal repetitions test and handgrip dynamometry; and quality of life by the Fibromyalgia Impact Questionnaire (FIQ), the Beck and Idate Trait-State Inventory (IDATE), a short-form health survey (SF-36). Results The STRE group was more effective to strength gain for all muscles and pain control after 4 and 16 weeks (p<0.05). The FLEX group showed higher improvements in anxiety (p<0.05). Both groups showed improvements in the QOL, and there was no significant difference observed between the groups. There was no change in the HRV of the STRE and FLEX groups. Conclusions Strengthening exercises show greater and more rapid improvements in pain and strength than flexibility exercises. Despite the benefits in fitness, pain, depression, anxiety and quality of life, no effect was observed on the autonomic modulation in both groups. This observation suggests that changes in autonomic modulation are not a target tobe clinically achieved in fibromyalgia. Trial Registration ClinicalTrials.gov NCT02004405
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Affiliation(s)
- Maria Bernadete Renoldi Oliveira Gavi
- Laboratory Assessment, Conditioning and Rehabilitation, Rheumatology Division, University Hospital of Federal University of Espirito Santo, Vitória-ES, Brazil
| | - Dalton Valentin Vassalo
- Post Graduation Program in Physiological Sciences, Federal University of Espírito Santo, Vitória-ES, Brazil
| | - Fabian Tadeu Amaral
- Laboratory Assessment, Conditioning and Rehabilitation, Physical Education and Federal University of Espirito Santo, Vitória-ES, Brazil
| | | | - Pablo Lúcio Gava
- Center of Health Sciences, Federal University of Espírito Santo, Vitória-ES, Brazil
| | - Eduardo Miranda Dantas
- Collegiate of Biological Sciences, Federal University of Vale do São Francisco, Petrolina-MG, Brazil
| | - Valéria Valim
- Rheumatology Division, University Hospital, Medicine Department, Federal University of Espírito Santo, Vitória-ES, Brazil
- * E-mail:
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22
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The inter-arm diastolic blood pressure difference induced by one arm ischemia: a new approach to assess vascular endothelia function. PLoS One 2014; 9:e84765. [PMID: 24454748 PMCID: PMC3890264 DOI: 10.1371/journal.pone.0084765] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/05/2013] [Accepted: 11/18/2013] [Indexed: 01/20/2023] Open
Abstract
OBJECTIVES To evaluate whether inter-arm diastolic blood pressure difference (DBPl-r) induced by one arm ischemia correlates with flow-mediated dilatation (FMD). METHODS Bilateral arm BPs were simultaneously measured with two automatic devices and right brachial artery diameter (D) was measured by ultrasound technique in 108 subjects (56 hypertensives and 52 normotensives). Following baseline diameter (D0) and BP measurement, right brachial artery was occluded for 5 minutes. The diameter was measured at 1, 1.5 and 2 min, and bilateral BPs measured at 3, 4 and 5 min after occlusion release. Their averages were recorded as post-D and post-BP, respectively. The difference between post-D and D0 (ΔD) was calculated as the percentage increase of artery diameter (ΔD/D0). The BP difference between left and right arms was calculated as BPl-r, and the difference of post- BPl-r and baseline BPl-r was recorded as the net change of BPl-r (ΔBPl-r). RESULTS At baseline, bilateral SBPs and DBPs were similar. Right arm ischemia induced significant DBP decline only in the right arm (68.8±12.7 vs 72.6±12.0 mmHg, P<0.05), which led to an increase of ΔDBPl-r (4.00±3.75 vs 0.78±4.47 mmHg, P<0.05). A positive correlation was seen between ΔD/D0 and ΔDBPl-r (r = 0.744, p<0.001). Furthermore, the correlation between age and ΔDBPl-r (r = -0.358, P<0.01) was similar to that between age and D/D0 (r = -0.398, P<0.01). Meanwhile, both ΔDBPl-r and ΔD/D0 were significantly lower in hypertensive patients than in normotensive patients. CONCLUSION The inter-arm DBP difference induced by one arm ischemia may be a potential index for clinical evaluation of vascular endothelial function.
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Roque FR, Hernanz R, Salaices M, Briones AM. Exercise training and cardiometabolic diseases: focus on the vascular system. Curr Hypertens Rep 2013; 15:204-14. [PMID: 23519745 DOI: 10.1007/s11906-013-0336-5] [Citation(s) in RCA: 45] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
The regular practice of physical activity is a well-recommended strategy for the prevention and treatment of several cardiovascular and metabolic diseases. Physical exercise prevents the progression of vascular diseases and reduces cardiovascular morbidity and mortality. Exercise training also ameliorates vascular changes including endothelial dysfunction and arterial remodeling and stiffness, usually present in type 2 diabetes, obesity, hypertension and metabolic syndrome. Common to these diseases is excessive oxidative stress, which plays an important role in the processes underlying vascular changes. At the vascular level, exercise training improves the redox state and consequently NO availability. Moreover, growing evidence indicates that other mediators such as prostanoids might be involved in the beneficial effects of exercise. The purpose of this review is to update recent findings describing the adaptation response induced by exercise in cardiovascular and metabolic diseases, focusing more specifically on the beneficial effects of exercise in the vasculature and the underlying mechanisms.
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Affiliation(s)
- Fernanda R Roque
- Departamento de Farmacología, Facultad de Medicina, Universidad Autónoma de Madrid, Instituto de Investigación Hospital Universitario La Paz (IdiPAZ), Madrid, Spain
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Fontes MT, Silva TLBT, Mota MM, Barreto AS, Rossoni LV, Santos MRV. Resistance exercise acutely enhances mesenteric artery insulin-induced relaxation in healthy rats. Life Sci 2013; 94:24-9. [PMID: 24316143 DOI: 10.1016/j.lfs.2013.11.017] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/03/2013] [Revised: 11/11/2013] [Accepted: 11/21/2013] [Indexed: 12/20/2022]
Abstract
AIMS We evaluated the mechanisms involved in insulin-induced vasodilatation after acute resistance exercise in healthy rats. MAIN METHODS Wistar rats were divided into 3 groups: control (CT), electrically stimulated (ES) and resistance exercise (RE). Immediately after acute RE (15 sets with 10 repetitions at 70% of maximal intensity), the animals were sacrificed and rings of mesenteric artery were mounted in an isometric system. After this, concentration-response curves to insulin were performed in control condition and in the presence of LY294002 (PI3K inhibitor), L-NAME (NOS inhibitor), L-NAME+TEA (K(+) channels inhibitor), LY294002+BQ123 (ET-A antagonist) or ouabain (Na(+)/K(+) ATPase inhibitor). KEY FINDINGS Acute RE increased insulin-induced vasorelaxation as compared to control (CT: Rmax=7.3 ± 0.4% and RE: Rmax=15.8 ± 0.8%; p<0.001). NOS inhibition reduced (p<0.001) this vasorelaxation from both groups (CT: Rmax=2.0 ± 0.3%, and RE: Rmax=-1.2 ± 0.1%), while PI3K inhibition abolished the vasorelaxation in CT (Rmax=-0.1±0.3%, p<0.001), and caused vasoconstriction in RE (Rmax=-6.5 ± 0.6%). That insulin-induced vasoconstriction on PI3K inhibition was abolished (p<0.001) by the ET-A antagonist (Rmax=2.9 ± 0.4%). Additionally, acute RE enhanced (p<0.001) the functional activity of the ouabain-sensitive Na(+)/K(+) ATPase activity (Rmax=10.7 ± 0.4%) and of the K(+) channels (Rmax=-6.1±0.5%; p<0.001) in the insulin-induced vasorelaxation as compared to CT. SIGNIFICANCE Such results suggest that acute RE promotes enhanced insulin-induced vasodilatation, which could act as a fine tuning to vascular tone.
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Affiliation(s)
- M T Fontes
- Department of Physiology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil
| | - T L B T Silva
- Department of Physiology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil
| | - M M Mota
- Department of Physiology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil
| | - A S Barreto
- Department of Physiology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil
| | - L V Rossoni
- Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900, São Paulo, SP, Brazil
| | - M R V Santos
- Department of Physiology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil.
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Campos JC, Gomes KMS, Ferreira JCB. Impact of exercise training on redox signaling in cardiovascular diseases. Food Chem Toxicol 2013; 62:107-19. [PMID: 23978413 DOI: 10.1016/j.fct.2013.08.035] [Citation(s) in RCA: 47] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/24/2013] [Revised: 08/05/2013] [Accepted: 08/18/2013] [Indexed: 02/07/2023]
Abstract
Reactive oxygen and nitrogen species regulate a wide array of signaling pathways that governs cardiovascular physiology. However, oxidant stress resulting from disrupted redox signaling has an adverse impact on the pathogenesis and progression of cardiovascular diseases. In this review, we address how redox signaling and oxidant stress affect the pathophysiology of cardiovascular diseases such as ischemia-reperfusion injury, hypertension and heart failure. We also summarize the benefits of exercise training in tackling the hyperactivation of cellular oxidases and mitochondrial dysfunction seen in cardiovascular diseases.
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Affiliation(s)
- Juliane C Campos
- Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
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Sousa N, Mendes R, Abrantes C, Sampaio J, Oliveira J. Long-term effects of aerobic training versus combined aerobic and resistance training in modifying cardiovascular disease risk factors in healthy elderly men. Geriatr Gerontol Int 2013; 13:928-35. [DOI: 10.1111/ggi.12033] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 12/11/2012] [Indexed: 01/07/2023]
Affiliation(s)
- Nelson Sousa
- Research Center in Sport Sciences, Health Sciences and Human Development; University of Trás-os-Montes e Alto Douro; Vila Real; Portugal
| | - Romeu Mendes
- Research Center in Sport Sciences, Health Sciences and Human Development; University of Trás-os-Montes e Alto Douro; Vila Real; Portugal
| | - Catarina Abrantes
- Research Center in Sport Sciences, Health Sciences and Human Development; University of Trás-os-Montes e Alto Douro; Vila Real; Portugal
| | - Jaime Sampaio
- Research Center in Sport Sciences, Health Sciences and Human Development; University of Trás-os-Montes e Alto Douro; Vila Real; Portugal
| | - José Oliveira
- Research Center in Physical Activity, Health and Leisure; Faculty of Sport; University of Porto; Porto; Portugal
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Abstract
Successful aging encompasses more than just the prevention of disease and disability; the truly well-lived life is demonstrated by a sense of vitality and independence, freedom from bodily pain, and the continued involvement in meaningful activities. While physical inactivity and sedentary behaviors accelerate the aging process, deliberate exercise and other forms of activity delay and/or prevent the onset of age-related pathologies such as frailty, osteoporosis, sarcopenia, and cardiovascular disease. This review surveys the evidence that supports the position that physical activity is a necessary component for the development and maintenance of the physiological resources that are foundational to physical and cognitive functioning and ‘living well’ in one's later years.
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Panveloski-Costa AC, Papoti M, Moreira RJ, Seraphim PM. Respostas lactacidêmicas de ratos ao treinamento intermitente de alta intensidade. REV BRAS MED ESPORTE 2012. [DOI: 10.1590/s1517-86922012000200012] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022] Open
Abstract
Durante contrações musculares de alta intensidade intervaladas por curtos períodos de tempo há importante participação do metabolismo glicolítico e, consequentemente, aumento das concentrações de lactato sanguíneo. O objetivo do estudo foi avaliar as respostas lactacidêmicas agudas e crônicas de ratos Wistar submetidos a um treinamento intermitente de alta intensidade (salto tipo jump squat) de três sessões semanais, a cada 24h, três séries de 12 repetições com intervalos de 60s entre cada uma. Houve aumento das concentrações de lactato sanguíneo durante a sessão aguda do treinamento (lactacidemia basal vs. lactacidemia após último esforço, P < 0,001). Contrariamente, após seis semanas de treinamento, ocorreu redução de 49% na resposta lactacidêmica ao exercício em relação à primeira sessão, P = 0,0002. O exercício intermitente de alta intensidade intervalado favorece a participação do sistema glicolítico; no entanto, o treinamento intermitente de alta intensidade promove redução das respostas lactacidêmicas, sugerindo melhora da capacidade de ressíntese de fosfocreatina e da biogênese mitocondrial.
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Guindani G, Umpierre D, Grigoletti SS, Vaz M, Stein R, Ribeiro JP. Blunted local but preserved remote vascular responses after resistance exercise in chronic heart failure. Eur J Prev Cardiol 2011; 19:972-82. [DOI: 10.1177/1741826711418931] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 01/27/2023]
Affiliation(s)
- Grace Guindani
- Exercise Pathophysiology Research Laboratory and Cardiology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
| | - Daniel Umpierre
- Exercise Pathophysiology Research Laboratory and Cardiology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
| | - Shana S Grigoletti
- Exercise Pathophysiology Research Laboratory and Cardiology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
| | - Marco Vaz
- Exercise Research Laboratory, School of Physical Education, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
| | - Ricardo Stein
- Exercise Pathophysiology Research Laboratory and Cardiology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
| | - Jorge P Ribeiro
- Exercise Pathophysiology Research Laboratory and Cardiology Division, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil
- Department of Medicine, Faculty of Medicine, Federal University of Rio Grande Sul, Porto Alegre, Brazil
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