Cancer and age related colonic crypt deficiencies in cytochrome c oxidase I

Table 1 Tumor marginal cytochrome c oxidase I deficiency, cluster size and tumor cytochrome c oxidase I deficiency

Patient No.	Total No. of crypts at margin of tumor in tissue section	No. of CcOI deficient crypts at margin of tumor	% of crypts at margin of tumor that are CcOI deficient [100 × (CcOI-deficient crypts/total crypts)]	Maximum cluster size (No. of deficient crypts adjacent to each other) of deficient crypts	Approximate % of area in tumor with CcOI deficiency
1	772	765	99	410	40
2	194	189	97	189	3
3	38	37	97	37	80
4	61	58	95	39	2
5	145	137	94	81	30
6	131	115	88	94	N/A1
7	56	45	80	20	5
8	43	31	72	4	2
9	31	22	71	7	2
10	15	10	67	8	1
11	24	16	66	3	15
12	126	82	65	32	4
13	17	9	53	3	28
14	59	20	34	6	10
15	47	16	34	5	7
16	10	3	30	2	1
17	244	68	29	11	50
18	341	92	27	6	1
19	126	34	27	12	10
20	133	33	25	5	18
21	162	35	22	4	10
22	286	63	22	6	N/A
23	72	16	22	4	3
24	892	181	20	8	N/A
25	237	44	19	5	20
26	633	111	18	16	15
27	120	19	16	5	N/A
28	634	97	15	5	8
29	1813	262	14	14	N/A
30	61	7	11	2	2
31	239	26	11	4	35
32	396	40	10	4	N/A
33	79	7	9	1	5
34	80	6	8	3	N/A
35	140	10	7	3	50
Overall average percent			42.1		16.4

¹N/A indicates that no tumor area was available in the tissue section as cut, and only the margin of the tumor was present. CcOI: Cytochromecoxidase I.

Table 2 Distribution of cytochrome c oxidase I defective crypts according to cluster size

Gender	(a) size of cluster	(b) number of defective clusters of each cluster size	(a) × (b) number of defective crypts for each cluster size	% of total crypts in each size class
Female	1	309	309	43.5
	2	82	164	23.1
	3	38	114	16
	4	15	60	8.4
	5	9	45	6.3
	6	2	12	1.7
	7	1	7	1
		Total 456	Total 711	Total 100.0
Male	1	622	622	35.2
	2	223	446	25.3
	3	95	285	16.1
	4	31	124	7
	5	20	100	5.7
	6	7	42	2.4
	7	5	35	2
	8	5	40	2.3
	9	1	9	0.5
	10	2	20	1.1
	12	1	12	0.7
	13	1	13	0.7
	17	1	17	1
		Total 1014	Total 1765	Total 100.0

Table 3 Correlations among non-steroidal anti-inflammatory drugs, vitamins, minerals, meat, age, cytochrome c oxidase I deficiency

Gender			NSAIDs	Vitamins	Minerals	Meat	Age	Average CcOI deficiency
Female	NSAIDs	Pearson correlation	1	0.242b	0.160a	0.068	0.220b	0.13
		Sig. (2-tailed)		0.002	0.046	0.402	0.006	0.111
		N	155	155	155	155	155	151
	Vitamins	Pearson correlation	0.242b	1	0.682b	-0.1	0.223b	0.191a
		Sig. (2-tailed)	0.002		0	0.215	0.005	0.019
		N	155	155	155	155	155	151
	Minerals	Pearson correlation	0.160a	0.682b	1	-0.127	0.191a	0.111
		Sig. (2-tailed)	0.046	0		0.114	0.017	0.177
		N	155	155	155	155	155	151
	Meat	Pearson correlation	0.068	-0.1	-0.127	1	-0.036	-0.044
		Sig. (2-tailed)	0.402	0.215	0.114		0.656	0.588
		N	155	155	155	155	155	151
	Age	Pearson correlation	0.220b	0.223b	0.191a	-0.036	1	0.582b
		Sig. (2-tailed)	0.006	0.005	0.017	0.656		0
		N	155	155	155	155	158	153
	Average CcOI	Pearson correlation	0.13	0.191a	0.111	-0.044	0.582b	1
	deficiency	Sig. (2-tailed)	0.111	0.019	0.177	0.588	0
		N	151	151	151	151	153	153
Male	NSAIDs	Pearson correlation	1	0.173a	0.112	0.1	0.184	0.119
		Sig. (2-tailed)		0.014	0.117	0.16	0.009	0.099
		N	200	199	199	200	200	192
	Vitamins	Pearson correlation	0.173a	1	0.576b	-0.062	0.195b	0.184a
		Sig. (2-tailed)	0.014		0	0.384	0.006	0.01
		N	199	201	201	200	201	193
	Minerals	Pearson correlation	0.112	0.576b	1	-0.111	0.198b	0.166a
		Sig. (2-tailed)	0.117	0		0.118	0.005	0.021
		N	199	201	201	200	201	193
	Meat	Pearson correlation	0.1	-0.062	-0.111	1	-0.105	-0.087
		Sig. (2-tailed)	0.16	0.384	0.118		0.138	0.228
		N	200	200	200	201	201	193
	Age	Pearson correlation	0.184	0.195b	0.198b	-0.105	1	0.663b
		Sig. (2-tailed)	0.009	0.006	0.005	0.138		0
		N	200	201	201	201	203	195
	Average	Pearson correlation	0.119	0.184a	0.166a	-0.087	0.663b	1
	CcOI	Sig. (2-tailed)	0.099	0.01	0.021	0.228	0
	deficiency	N	192	193	193	193	195	195

^aCorrelation is significant at the 0.05 level (2-tailed);

^bCorrelation is significant at the 0.01 level (2-tailed). NSAIDs: Non-steroidal anti-inflammatory drugs; CcOI: Cytochrome c oxidase I.